LOCUS       sequence-405699-        2513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 22357 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-glna
FEATURES             Location/Qualifiers
     source          1..2513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(844..1432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(924..943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1523..2314)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa
       61 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc
      121 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt
      181 gattttgtgc cgagctcatt actcgcatcc attctcaggc tgtctcgtct cgtctcaggt
      241 ctcagcttaa aaagagacaa ccgtattcga acaaggaatg acagtgaaat aattttggaa
      301 ggttctccgt gacaaccggt gtgttggttc gaagactgct tattttttcg cgagagcgcc
      361 agttacgtcc gattttcgta tatttttgaa ttacaagcat tgtactgcaa caaagcattt
      421 gctgcctgac gtttagttaa ttcgttagga ttagaaggca acggccgttt gagctatcca
      481 aacacagcaa acacggctgt cgtgatcgta agaaccattg tagaccattc cttcgccctg
      541 tcctctgagc tggatgtgag gccgttcaaa atttcgtcaa cgtcgacact aatatcggca
      601 ttgggaaagc taccgttgcc atcggaatag ctcacagttc gagccgccac aaactcgacc
      661 gattctgcga cgacggtaga ccgcgtcaat aggttgacgg acgctcgaga ccaccaggat
      721 acatagatta ccacaactcc gagcccttcc acccacagaa tcaggggata acgcaggaaa
      781 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1021 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1261 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1381 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1441 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1501 ggtcatgcat tctaggtgat tattatttgc cgactacctt ggtgatctcg cctttcacgt
     1561 agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa
     1621 gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg
     1681 cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc
     1741 gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata
     1801 gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt
     1861 cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag
     1921 ccagatcaat gtcgatcgtg gctggctcga agataccagc aagaatgtca ttgcgctgcc
     1981 attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca
     2041 caacaatggt gacttctaca gcgcggagaa tctcactctc tccaggggaa gccgaagttt
     2101 ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa
     2161 ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat
     2221 gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt
     2281 gagtcgatac ttcggcgatc accgcttccc tcatgcgaaa cgatcctcat cctgtctctt
     2341 gatcagatat tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
     2401 ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg
     2461 ctaagcttgc atgcctgcaa gtcgactcta gaggagcggt gatcacaggc agc
//
