LOCUS       sequence-405701-        2560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 55209 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-epyC
FEATURES             Location/Qualifiers
     source          1..2560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(978..1566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1058..1077)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1657..2448)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gagggcgccc cagctggcaa ttccggttcg cttgctaagc ttgcatgcct gcaagtcgac
       61 tctagaggag cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
      121 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
      181 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
      241 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctcattact cgcatccatt
      301 ctcaggctgt ctcgtctcgt ctcaggtctc agcttgtagg cccatagttg ctaatttcga
      361 atattccttt cgaatcgacg cagccatggc acaatggtgg tgcttgaaga ctaacagtaa
      421 gcaaagatga ctctttagat tttaaaacca aaatggtgct tagctaactg taagaatgtt
      481 agatttacat ataagcaatt accattcttt gtaactacat atctgccgta ctccagcgat
      541 acgcctgctc attttgatgc tcatggttgt agcgctgtga caataactta tcttcccaat
      601 catttccacg gtccattcca tcccatcgga agccaggccg aatcccgtag cggtttggtt
      661 ttgctgatgg acccttgtac acggggcgta catgaagcat gccattggaa tttgcttccg
      721 tcttttgctg ttggttttga gctgtataag ctgccattgg atctccaact cgaatggcct
      781 ccttgcgcgc ggcttccaat tcggagtccc cggcgtgtcg tgcaaacgat gagttcgctc
      841 gagaccacca ggatacatag attaccacaa ctccgagccc ttccacccac agaatcaggg
      901 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      961 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1021 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1081 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1141 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1201 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1261 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1321 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1381 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1441 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1501 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1561 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1621 cgttaaggga ttttggtcat gcattctagg tgattattat ttgccgacta ccttggtgat
     1681 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1741 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1801 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1861 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1921 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1981 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     2041 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac cagcaagaat
     2101 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     2161 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcac tctctccagg
     2221 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     2281 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2341 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2401 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatgc gaaacgatcc
     2461 tcatcctgtc tcttgatcag atattgatcc cctgcgccat cagatccttg gcggcaagaa
     2521 agccatccag tttactttgc agggcttccc aaccttacca
//
