LOCUS       sequence-405704-       11298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression PIE-PUM2 fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Ruan X, Hu K, Zhang X
  TITLE     PIE-seq: identifying RNA-binding protein targets by dual 
            RNA-deaminase editing and sequencing.
  JOURNAL   Nat Commun. 2023 Jun 6;14(1):3275. doi: 10.1038/s41467-023-39054-8.
  PUBMED    37280234
REFERENCE   2  (bases 1 to 11298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-06-6"; volume: "14(1):3275. doi"; pages: " 
            10.1038/s41467-023-39054-8"
COMMENT     Sequence Label: pR023_PIE-PUM2
FEATURES             Location/Qualifiers
     source          1..11298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1736..1762
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1763..1786
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1796..2479
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     misc_feature    join(6950..7524,7525..7527,7528..7536)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7117..7134)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7344..7363
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7537..7539,7540..7542,7543..8256)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7537..7539,7540..7542,7543..8253)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7582..7603)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7843..7862)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8190..8211
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8320..8339)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8385..8440
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8439..8458)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8801..8817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8801..8817)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8814..8836)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8825..8841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8849..8855,8856..8873,8874..8879))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8894..8915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8974..9169
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9020..9155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9175..9309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9212..9231)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9266..9285
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9377..9394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9548..10136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9628..9647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10307..11098,11099..11167))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10930..10949
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11168..11272)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggsgc gccrgggggg gggggggggg gggggggggg
      541 ggggggggsg rggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gkgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg rgggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcatgtaccc
     1741 atacgacgtc ccagactacg ctgactacaa agacgatgac gacaagggcg cgcctagctc
     1801 agagactggc ccagtggctg tggaccccac attgagacgg cggatcgagc cccatgagtt
     1861 tgaggtattc ttcgatccga gagagctccg caaggagacc tgcctgcttt acgaaattaa
     1921 ttgggggggc cggcactcca tttggcgaca tacatcacag aacactaaca agcacgtcga
     1981 agtcaacttc atcgagaagt tcacgacaga aagatatttc tgtccgaaca caaggtgcag
     2041 cattacctgg tttctcagct ggagcccatg cggcgaatgt agtagggcca tcactgaatt
     2101 cctgtcaagg tatccccacg tcactctgtt tatttacatc gcaaggctgt accaccacgc
     2161 tgacccccgc aatcgacaag gcctgcggga tttgatctct tcaggtgtga ctatccaaat
     2221 tatgactgag caggagtcag gatactgctg gagaaacttt gtgaattata gcccgagtaa
     2281 tgaagcccac tggcctaggt atccccatct gtgggtacga ctgtacgttc ttgaactgta
     2341 ctgcatcata ctgggcctgc ctccttgtct caacattctg agaaggaagc agccacagct
     2401 gacattcttt accatcgctc ttcagtcttg tcattaccag cgactgcccc cacacattct
     2461 ctgggccacc gggttgaaaa gcggcagcga gactcccggg acctcagagt ccgccacacc
     2521 cgaaagtatc gataatcatg attttcaagc tcttgcatta gaatctcggg gaatgggaga
     2581 gcttttgcct accaaaaagt tttgggaacc tgatgattca acaaaagatg gacaaaaagg
     2641 catatttctt ggggatgatg aatggagaga gactgcatgg ggagcttctc accattcaat
     2701 gtcccagcct attatggtac agagaagatc tggacagggt tttcatggaa acagtgaagt
     2761 aaatgcaata ctgtctccgc gatcagaaag tggaggcctt ggtgtgagca tggtagaata
     2821 tgtattaagt tcttctcctg ctgataaatt ggattctcga tttaggaagg gaaattttgg
     2881 cactagagat gctgaaacag atggacctga gaaaggagat caaaaaggca aggcttctcc
     2941 atttgaggag gaccaaaaca gagaccttaa acaaggagat gatgatgatt ctaaaataaa
     3001 tggcagaggt ttgccaaatg gaatggatgc cgattgcaaa gattttaatc gtactcctgg
     3061 aagtcgtcaa gcctctccaa ctgaagtagt tgagcgcttg ggccccaata ctaatccctc
     3121 agaaggactg gggcctcttc ctaatcctac agctaataaa ccacttgttg aagaattttc
     3181 aaatcctgaa actcagaatc tggatgccat ggaacaagtt ggtctggaat ccttacagtt
     3241 tgactatcct ggtaatcagg taccaatgga ctcttcagga gctactgtag gcctttttga
     3301 ctacaattcc cagcagcagc tctttcagag gactaatgca ctaacagttc aacagttaac
     3361 tgcagctcaa cagcagcaat atgcattagc agcagctcag cagccacata tagctggtgt
     3421 attctcagca ggccttgctc cagctgcatt tgtgccaaat ccatacatta ttagtgctgc
     3481 tcctccaggg accgatccgt atactgcagc aggattggct gcagcagcta cattagcagg
     3541 tccagcagtg gttccacctc agtattacgg cgttccatgg ggggtgtatc cagccaactt
     3601 atttcagcag caagctgcag ctgcggcaaa taacacagcc agtcagcaag cagcatcaca
     3661 agctcagcct ggacagcaac aggttctccg tgctggagca ggtcagcgtc ctcttactcc
     3721 caatcagggt cagcaagggc agcaagcaga atcacttgcg gcagctgcag cagcaaatcc
     3781 aacattggct tttggtcagg gtcttgctac tggcatgcca ggctatcaag tactagctcc
     3841 aactgcctat tatgatcaga ctggtgcctt agtggttggc cctggagcaa ggactggcct
     3901 tggagctcca gttcggttaa tggctccaac acctgtttta attagttcag cagcagcaca
     3961 agctgcagca gcagcagcag ctggaggaac tgcaagtagc cttacaggca gcacaaatgg
     4021 tctgtttcgg ccaattggca ctcagccacc acagcagcag caacagcagc caagcactaa
     4081 tctgcaatct aattcatttt atggaagcag ttctttgact aatagctccc agagtagttc
     4141 tttattttct catggacctg gtcaacctgg aagtacatct cttggctttg gaagtggtaa
     4201 ctctttgggt gctgctatag gctcagccct cagtggattt ggttcatcag ttggcagttc
     4261 tgcaagtagt agtgccacaa ggagagagtc tctatctact agctctgact tgtacaaaag
     4321 atctagtagc agcctagcac ccatagggca accattttac aatagtctgg gattttcctc
     4381 ctctccaagt ccaataggca tgcctctgcc aagccaaact ccaggacatt cacttacgcc
     4441 accgccatca ctttcatcac atggatcctc atccagtttg catttaggag gactgacaaa
     4501 tggtagtggt cgatatatct ctgcagcacc tggagcagaa gcaaaatatc gaagtgcttc
     4561 aagcacttcc agtctattta gctccagcag ccagctcttt cctccttccc ggcttcggta
     4621 taataggtct gatattatgc cttctggccg cagtagatta ttggaagatt tcagaaacaa
     4681 ccgcttccca aaccttcagc ttagagactt gattggacat atagttgagt tttctcaaga
     4741 ccagcatggt tctagattca tacagcaaaa actagagaga gctactccag ctgagcgaca
     4801 gatggtattt aatgaaattc tgcaagcagc ctatcaatta atgactgatg tttttggcaa
     4861 ctatgttata cagaagtttt ttgagtttgg gagtctggat caaaaattag ccctggctac
     4921 tcgtattcgt ggtcatgttc tacccttagc cttgcagatg tatggctgcc gcgttattca
     4981 gaaagcatta gaatctattt cttctgacca gcagagtgaa atggtaaagg agctggatgg
     5041 tcatgtgctc aaatgtgtga aagatcagaa tggaaaccat gttgtacaaa aatgtatcga
     5101 atgtgttcag ccacagtcac tacagttcat cattgatgct ttcaagggac aagtatttgt
     5161 gctttcaact catccttatg gctgcagagt aattcagcgc atcctagagc attgcactgc
     5221 agaacagacc ttacctatct tagaagaact ccaccaacat acagagcagt tggtacagga
     5281 tcagtatggc aattatgtta ttcagcatgt actggaacac ggtcgacctg aagacaagag
     5341 caaaattgtt tccgaaatca ggggaaaggt tttagccctg agtcaacaca aatttgccag
     5401 caatgtagta gaaaagtgtg ttactcatgc ctcccgtgct gagagagctt tactgattga
     5461 cgaggtttgc tgccagaatg atggtcctca cagtgcctta tacaccatga tgaaggacca
     5521 gtatgccaat tacgtggttc aaaagatgat tgatatggct gaacctgctc agagaaagat
     5581 aatcatgcac aagattcgac ctcacattac tactttgcgc aaatacacat acgggaagca
     5641 tatactggcc aagttggaaa agtattattt gaagaatagc ccggacctag gacctattgg
     5701 aggaccacca aatggaatgc tggatatcgg aggttccggc ggatctggtg gatcaggtgg
     5761 acagctgcat ttaccgcagg ttttagctga cgctgtctca cgcctggtcc tgggtaagtt
     5821 tggtgacctg accgacaact tctcctcccc tcacgctcgc agaaaagtgc tggctggagt
     5881 cgtcatgaca acaggcacag atgttaaaga tgccaaggtg ataagtgttt ctacaggagg
     5941 caaatgtatt aatggtgaat acatgagtga tcgtggcctt gcattaaatg actgccatgc
     6001 agaaataata tctcggagat ccttgctcag atttctttat acacaacttg agctttactt
     6061 aaataacaaa gatgatcaaa aaagatccat ctttcagaaa tcagagcgag gggggtttag
     6121 gctgaaggag aatgtccagt ttcatctgta catcagcacc tctccctgtg gagatgccag
     6181 aatcttctca ccacatgagc caatcctgga agaaccagca gatagacacc caaatcgtaa
     6241 agcaagagga cagctacgga ccaaaataga gtctggtcag gggacgattc cagtgcgctc
     6301 caatgcgagc atccaaacgt gggacggggt gctgcaaggg gagcggctgc tcaccatgtc
     6361 ctgcagtgac aagattgcac gctggaacgt ggtgggcatc cagggatcac tgctcagcat
     6421 tttcgtggag cccatttact tctcgagcat catcctgggc agcctttacc acggggacca
     6481 cctttccagg gccatgtacc agcggatctc caacatagag gacctgccac ctctctacac
     6541 cctcaacaag cctttgctca gtggcatcag caatgcagaa gcacggcagc cagggaaggc
     6601 ccccaacttc agtgtcaact ggacggtagg cgactccgct attgaggtca tcaacgccac
     6661 gactgggaag gatgagctgg gccgcgcgtc ccgcctgtgt aagcacgcgt tgtactgtcg
     6721 ctggatgcgt gtgcacggca aggttccctc ccacttacta cgctccaaga ttaccaagcc
     6781 caacgtgtac catgagtcca agctggcggc aaaggagtac caggccgcca aggcgcgtct
     6841 gttcacagcc ttcatcaagg cggggctggg ggcctgggtg gagaagccca ccgagcagga
     6901 ccagttctca ctcacgtaag cggccgctac gtaaattccg cccccccccc ccctctccct
     6961 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     7021 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     7081 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     7141 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     7201 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     7261 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     7321 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     7381 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     7441 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     7501 tcctttgaaa aacacgatga taatatggcc acaaccatgg tgagcaaggg cgaggagctg
     7561 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     7621 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     7681 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     7741 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     7801 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     7861 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     7921 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     7981 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     8041 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     8101 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     8161 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     8221 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccggccgcc agcacagtgg
     8281 tcgatcgacc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     8341 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     8401 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     8461 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     8521 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     8581 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     8641 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     8701 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     8761 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     8821 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8881 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8941 tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt
     9001 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9061 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg
     9181 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9241 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9301 gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9361 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9421 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9481 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9541 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9601 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9661 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9721 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9781 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9841 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9901 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9961 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    10021 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10081 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10141 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10201 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10261 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10321 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10381 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10441 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10501 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10561 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10621 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10681 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10741 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10801 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10861 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10921 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10981 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11041 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11101 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11161 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11221 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11281 cccgaaaagt gccacctg
//
