LOCUS       sequence-405712-        7711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Yellow Fluorescent Protein (YFP) in Listeria 
            monocytogenes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Andersen JB, Roldgaard BB, Lindner AB, Christensen BB, Licht TR
  TITLE     Construction of a multiple fluorescence labelling system for use in 
            co-invasion studies of Listeria monocytogenes.
  JOURNAL   BMC Microbiol. 2006 Oct 3;6:86. doi: 10.1186/1471-2180-6-86.
  PUBMED    17014739
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2006-10-3"; volume: "6:86. doi"; pages: " 
            10.1186/1471-2180-6-86"
COMMENT     Sequence Label: PJEBAN3
FEATURES             Location/Qualifiers
     source          1..7711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     197..216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     450..467
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    584..605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(620..625,626..643,644..650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    658..674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     663..685
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     682..698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     682..698
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(1174..1176,1177..1179,1180..1893)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITRGMDELYK"
     primer_bind     complement(1207..1235)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1830..1850
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1915..1932)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1915..1931)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1924..1946)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(2257..2628)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            2661..2770
                     /note="incP origin of transfer"
     rep_origin      join(7419..7711,1..296)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
       61 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      121 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      181 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      241 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
      301 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      361 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      421 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      481 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      541 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      601 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      661 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg
      721 taccccccgt tccaagaccc acaaccattc catctttaat ccattcacaa gccttttctc
      781 ctgcaatctt cttttgatta atcatagtcc aacctctttt ctagcattaa aacattgcta
      841 tatctattgt ttcattatac tcgtacctac tttcaagttc aaaatatgtt tatttttcta
      901 accttacaaa aatgtaatta gacatttaaa gacaaatgta ttacagtaag taagtattaa
      961 acgtttattt ttaatatttg taacactaat gaaacaatga agaggtggat ttatgaataa
     1021 aataaaacta attttgcatc atccagcaac tatttttaca atgaaaactt ttttctacct
     1081 agctattttg cttggattgc tttggtttta tggtttcaaa aaccctgaag gtggaaattc
     1141 atttacaacg gatccgaaag gaggtttatt aaaatggcta gcaaaggaga agaacttttc
     1201 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1261 gtcagtggag agggtgaagg tgatgctaca tacggaaagc ttacccttaa actgatttgc
     1321 actactggaa aactacctgt tccatggcca acacttgtca ctactttggg ctatggtctt
     1381 caatgctttg cccgttatcc ggatcatatg aaacggcatg actttttcaa gagtgccatg
     1441 cccgaaggtt atgtacagga acgcactata tctttcaaag atgacgggaa ctacaagacg
     1501 cgtgctgaag tcaagtttga aggtgatacc cttgttaatc gtatcgagtt aaaaggtatt
     1561 gattttaaag aagatggaaa cattctcgga cacaaactcg agtacaacta taactcacac
     1621 aatgtataca tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattcgc
     1681 cacaacattg aagatggatc cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     1741 ggcgatggcc ctgtcctttt accagacaac cattacctgt cgtatcaatc tgccctttcg
     1801 aaagatccca acgaaaagcg tgaccacatg gtccttcttg agtttgtaac tgctgctggg
     1861 attacacgtg gcatggatga gctctacaaa taactgcagg catgcaagct tggcactggc
     1921 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     1981 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2041 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     2101 tctgtgcggt atttcacacc gcatagggga tcggtcttgc cttgctcgtc ggtgatgtac
     2161 ttcaccagct ccgcgaagtc gctcttcttg atggagcgca tggggacgtg cttggcaatc
     2221 acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat ggctctgccc tcgggcggac
     2281 cacgcccatc atgaccttgc caagctcgtc ctgcttctct tcgatcttcg ccagcagggc
     2341 gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg tcggtgagcc agagtttcag
     2401 caggccgccc aggcggccca ggtcgccatt gatgcgggcc agctcgcgga cgtgctcata
     2461 gtccacgacg cccgtgattt tgtagccctg gccgacggcc agcaggtagg ccgacaggct
     2521 catgccggcc gccgccgcct tttcctcaat cgctcttcgt tcgtctggaa ggcagtacac
     2581 cttgataggt gggctgccct tcctggttgg cttggtttca tcagccatcc gcttgccctc
     2641 atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
     2701 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
     2761 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
     2821 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
     2881 gaagcagggt tatgcagcgg aaaagatccc ccgaagcaaa cttaagagtg tgttgatagt
     2941 gcagtatctt aaaattttgt gtataatagg aattgaagtt aaattagatg ctaaaaattt
     3001 gtaattaaga aggagggatt cgtcatgttg gtattccaaa tgcgtaatgt agataaaaca
     3061 tctactgttt tgaaacagac taaaaacagt gattacgcag ataaataaat acgttagatt
     3121 aattcctacc agtgactaat cttatgactt tttaaacaga taactaaaat tacaaacaaa
     3181 tcgtttaact tctgtattta tttacagatg taatcacttc aggagtaatt acatgaacaa
     3241 aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc aaataataaa
     3301 acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta aagggcattt
     3361 aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag acagtcatct
     3421 attcaactta tcgtcagaaa aattaaaact gaacattcgt gtcactttaa ttcaccaaga
     3481 tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga gtattcctta
     3541 ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta
     3601 tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa cactagggtt
     3661 gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg aatgctttca
     3721 tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca cagatgttcc
     3781 agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg
     3841 tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag taaacaattt
     3901 aagtaccatt acttatgagc aagtattgtc tatttttaat agttatctat tatttaacgg
     3961 gaggaaataa ttctatgagt cgctttttta aatttggaaa gttacacgtt actaaaggga
     4021 atggagataa attattagat atactactga cagcttccaa gaagctaaag aggtccctag
     4081 cgcctacggg gaatttgggc aaatcaaaag aaaaattctc ccaagaatta gaaatgagta
     4141 gatcaaatta ttcacgaata gaatcaggaa aatcagatcc aaccataaaa acactagaac
     4201 aaattgcaaa gttaactaac tcaacgctag tagtggattt aatcccaaat gagccaacag
     4261 aaccagaacc agaaacagaa tcagaacaag taacattgga tttagaaatg gaagaagaaa
     4321 aaagcaatga cttcgtgtga ataatgcacg aaatcgttgc ttattttttt taaaagcggt
     4381 atactagata taacgaaaca acgaactgaa tagaaacgaa aaaagagcca tgacacattt
     4441 ataaaatgtt tgacgacatt ttataaatgc atagcccgat aagattgcca aaccaacgct
     4501 tatcagttag tcagatgaac tcttccctcg taagaagtta tttaattaac tttgtttgaa
     4561 gacggtatat aaccgtacta tcattatata gggaaatcag agagttttca agtatctaag
     4621 ctactgaatt taagaattgt taagcaatca atcggaaatc gtttgattgc tttttttgta
     4681 ttcatttata gaaggtggag tttgtatgaa tcatgatgaa tgtaaaactt atataaaaaa
     4741 tagtttattg gagataagaa aattagcaaa tatctataca ctagaaacgt ttaagaaaga
     4801 gttagaaaag agaaatatct acttagaaac aaaatcagat aagtattttt cttcggaggg
     4861 ggaagattat atatataagt taatagaaaa taacaaaata atttattcga ttagtggaaa
     4921 aaaattgact tataaaggaa aaaaatcttt ttcaaaacat gcaatattga aacagttgaa
     4981 tgaaaaagca aaccaagtta attaaacaac ctattttata ggatttatag gaaaggagaa
     5041 cagctgaatg aatatccctt ttgttgtaga aactgtgctt catgacggct tgttaaagta
     5101 caaatttaaa aatagtaaaa ttcgctcaat cactaccaag ccaggtaaaa gcaaaggggc
     5161 tatttttgcg tatcgctcaa aatcaagcat gattggcggt cgtggtgttg ttctgacttc
     5221 cgaggaagcg attcaagaaa atcaagatac atttacacat tggacaccca acgtttatcg
     5281 ttatggaacg tatgcagacg aaaaccgttc atacacgaaa ggacattctg aaaacaattt
     5341 aagacaaatc aataccttct ttattgattt tgatattcac acggcaaaag aaactatttc
     5401 agcaagcgat attttaacaa ccgctattga tttaggtttt atgcctacta tgattatcaa
     5461 atctgataaa ggttatcaag catattttgt tttagaaacg ccagtctatg tgacttcaaa
     5521 atcagaattt aaatctgtca aagcagccaa aataatttcg caaaatatcc gagaatattt
     5581 tggaaagtct ttgccagttg atctaacgtg taatcatttt ggtattgctc gcataccaag
     5641 aacggacaat gtagaatttt ttgatcctaa ttaccgttat tctttcaaag aatggcaaga
     5701 ttggtctttc aaacaaacag ataataaggg ctttactcgt tcaagtctaa cggttttaag
     5761 cggtacagaa ggcaaaaaac aagtagatga accctggttt aatctcttat tgcacgaaac
     5821 gaaattttca ggagaaaagg gtttaatagg gcgtaataac gtcatgttta ccctctcttt
     5881 agcctacttt agttcaggct attcaatcga aacgtgcgaa tataatatgt ttgagtttaa
     5941 taatcgatta gatcaaccct tagaagaaaa agaagtaatc aaaattgtta gaagtgccta
     6001 ttcagaaaac tatcaagggg ctaataggga atacattacc attctttgca aagcttgggt
     6061 atcaagtgat ttaaccagta aagatttatt tgtccgtcaa gggtggttta aattcaagaa
     6121 aaaaagaagc gaacgtcaac gtgttcattt gtcagaatgg aaagaagatt taatggctta
     6181 tattagcgaa aaaagcgatg tatacaagcc ttatttagtg acgaccaaaa aagagattag
     6241 agaagtgcta ggcattcctg aacggacatt agataaattg ctgaaggtac tgaaggcgaa
     6301 tcaggaaatt ttctttaaga ttaaaccagg aagaaatggt ggcattcaac ttgctagtgt
     6361 taaatcattg ttgctatcga tcattaaagt aaaaaaagaa gaaaaagaaa gctatataaa
     6421 ggcgctgaca aattcttttg acttagagca tacattcatt caagagactt taaacaagct
     6481 agcagaacgc cctaaaacgg acacacaact cgatttgttt agctatgata caggctgaaa
     6541 ataaaacccg cactatgcca ttacatttat atctatgata cgtgtttgtt ttttctttgc
     6601 tgtttagcga atgattagca gaaatataca gagtaagatt ttaattaatt attaggggga
     6661 gaaggagaga gtagcccgaa aacttttagt tggcttggac tgaacgaagt gagggaaagg
     6721 ctactaaaac gtcgaggggc agtgagagcg aagcgaacac ttgatttttt aattttctat
     6781 cttttatagg tcattagagt atacttattt gtcctataaa ctatttagca gcataataga
     6841 tttattgaat aggtcattta agttgagcat attagaggag gaaaatcttg gagaaatatt
     6901 tgaagaaccc gattacatgg attggattag ttcttgtggt tacgtggttt ttaactaaaa
     6961 gtagtgaatt tttgattttt ggtgtgtgtg tcttgttgtt agtatttgct agtcaaagtg
     7021 attaaataga attgaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7081 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7141 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7201 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7261 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7321 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7381 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7441 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7501 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7561 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7621 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7681 cttaccgggt tggactcaag acgatagtta c
//
