LOCUS       sequence-405718-        9946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An integrating plasmid containing both the inducible enotetSW1 
            promoter and the coding sequence of the TetR repressor under the 
            control of the constitutive CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Lyu XH, Yang YS, Pan ZQ, Ning SK, Suo F, Du LL
  TITLE     An improved tetracycline-inducible expression system for fission 
            yeast.
  JOURNAL   J Cell Sci. 2024 Nov 1;137(21):jcs263404. doi: 10.1242/jcs.263404. 
            Epub 2024 Nov 1.
  PUBMED    39318285
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2024-11-1"; volume: "137(21):jcs263404. doi"; pages: "
            10.1242/jcs.263404. Epub 2024 Nov 1"
COMMENT     Sequence Label: pDB5319
FEATURES             Location/Qualifiers
     source          1..9946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2707..3162)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2844..2865)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3056..3075
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3288..3310
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3302..3319
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3303..3319
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3329..3348
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3329..3347
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(3357..3980)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     3888..3907
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4061..4085)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4082..4285)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4111..4131)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4286..4665
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    4973..4991
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(5082..5110)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5705..5725
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      5793..5980
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5848..5872)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            6049..7624
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        6049..6392
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6393..7421
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMRLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVGASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      7427..7624
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7686..7706)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7686..7704)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7725..7741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7725..7741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7738..7760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7749..7765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7773..7779,7780..7797,7798..7803))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7818..7839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7956..7973)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8127..8715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8207..8226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8886..9677,9678..9746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9509..9528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9747..9851)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9919..9937
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttagc ggccgctaaa cgttgcttta tatatggata
      601 acgacatata catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct
      661 gctttaaagt tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc
      721 aaaactcaaa taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat
      781 tcgttttttc aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca
      841 tcgaatatga gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg
      901 atggtagagg cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct
      961 cctgccaaac aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat
     1021 gcaattgttg aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac
     1081 actgatgcct tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg
     1141 cgatgcattc aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt
     1201 cctgaatttt gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt
     1261 tatccttttg tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt
     1321 catcaaccat ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt
     1381 gaaaagttcg ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga
     1441 aaagtttatg cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta
     1501 tatgcccctg ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat
     1561 gcggtgcgca cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat
     1621 ggtgagacca ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc
     1681 attgaagctt gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct
     1741 ttcagcgaaa tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt
     1801 ttaggtactg atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc
     1861 ggtgcaactt tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac
     1921 gttaccatca tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac
     1981 gtcaaagatc ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta
     2041 agcaagatga aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact
     2101 attgtttcag ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca
     2161 agagggttgc gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt
     2221 gctgcaatga cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga
     2281 gttgactctc ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct
     2341 atcaataata gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc
     2401 tcccttttgg ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag
     2461 gctgataaat tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata
     2521 actgttaaat gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga
     2581 ttgtaaaatg tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt
     2641 gctgcgacgc agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2701 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2761 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2821 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2881 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2941 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3001 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3061 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3121 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     3181 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     3241 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     3301 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattggttaa
     3361 gatccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc aaggccgaat
     3421 aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg taataatggc
     3481 ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg ctcttgatct
     3541 tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa tgcattctct
     3601 agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc atactgtttt
     3661 tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag taaagcacat
     3721 ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc taaagggcaa
     3781 aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa agcccgctta
     3841 ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc gagtttacgg
     3901 gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt aatcacttta
     3961 cttttatcta atctagacat atgaattcgg ggccgcggag gctggatcgg tcccggtgtc
     4021 ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac
     4081 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     4141 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     4201 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     4261 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     4321 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     4381 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     4441 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     4501 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     4561 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     4621 accccgtaat tgattactat taataactag tcaataatca atgtcaacat ggcggtaatg
     4681 ttggacatga gccaatataa atgtacatat tatgatatgg atacaacgta tgcaatgggc
     4741 caagctcctc gaccccccgg gccccatctc ttgccccttc taagctcgct ggactcgcac
     4801 attggctacg gtggctcgcg tcgctcgctt ggtcgctcct tcaacgtacc ctaacgttcc
     4861 ggttaacgat tcttaaagaa gcgaccaatt tgttccaccg gcttctgtgg gctcaggggc
     4921 atgtcatgga agattgagag gatcggcgct tcctccaggt atatatagag tgtccctatc
     4981 agtgatagag acttgtttca gtaagaatca attagtattc tacagtaaac atcggccgct
     5041 cgggcgctag catgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     5101 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     5161 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     5221 ggccaacact tgtcactact ttgggttatg gtctaatgtg ctttgctaga tacccagatc
     5281 atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     5341 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
     5401 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     5461 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
     5521 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     5581 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     5641 acaaccatta cctgtcctat caatctaagc tttcgaaaga tcccaacgaa aagagagacc
     5701 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     5761 acaaaagatc ttaacgcgcc acttctaaat aagcgaattt cttatgattt atgattttta
     5821 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     5881 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     5941 tatagtatga ggtcgctctt attgaccaca cctctaccgg cagatccgct agggataaca
     6001 gggtaatatg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
     6061 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
     6121 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc
     6181 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcgga cctgcgagca gggaaacgct
     6241 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt
     6301 taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac
     6361 agttctcaca tcacatccga acataaacaa ccatgggtaa aaagcctgaa ctcaccgcga
     6421 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcggctct
     6481 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     6541 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     6601 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     6661 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     6721 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     6781 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     6841 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     6901 ccgtcggtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     6961 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     7021 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     7081 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     7141 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     7201 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     7261 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     7321 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     7381 gaaaccgacg ccccagcact cgtccgaggg caaaggaata atcagtactg acaataaaaa
     7441 gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt
     7501 ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc
     7561 gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat
     7621 actgctgtcg attcgatact aacgccgcca tccacctgca ggtaatcacg tgccagcttt
     7681 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     7741 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7801 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7861 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7921 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7981 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8041 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8101 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8161 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8221 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8281 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8341 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8401 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8461 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8521 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8581 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8641 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8701 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8761 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9181 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9781 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9841 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9901 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
