LOCUS       sequence-405723-        9950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An integrating plasmid containing both the inducible enotetSW3 
            promoter and the coding sequence of the TetR repressor under the 
            control of the constitutive CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Lyu XH, Yang YS, Pan ZQ, Ning SK, Suo F, Du LL
  TITLE     An improved tetracycline-inducible expression system for fission 
            yeast.
  JOURNAL   J Cell Sci. 2024 Nov 1;137(21):jcs263404. doi: 10.1242/jcs.263404. 
            Epub 2024 Nov 1.
  PUBMED    39318285
REFERENCE   2  (bases 1 to 9950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2024-11-1"; volume: "137(21):jcs263404. doi"; pages: "
            10.1242/jcs.263404. Epub 2024 Nov 1"
COMMENT     Sequence Label: pDB5321
FEATURES             Location/Qualifiers
     source          1..9950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2707..3162)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2844..2865)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3056..3075
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3288..3310
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3302..3319
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3303..3319
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3329..3348
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3329..3347
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(3357..3980)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     3888..3907
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4061..4085)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4082..4285)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4111..4131)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4286..4665
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    4973..4991
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(5086..5114)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5709..5729
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      5797..5984
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5852..5876)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            6053..7628
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        6053..6396
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6397..7425
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMRLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVGASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      7431..7628
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7690..7710)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7690..7708)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7729..7745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7729..7745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7742..7764)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7753..7769
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7777..7783,7784..7801,7802..7807))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7822..7843
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7960..7977)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8131..8719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8211..8230)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8890..9681,9682..9750))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9513..9532
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9751..9855)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9923..9941
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttagc ggccgctaaa cgttgcttta tatatggata
      601 acgacatata catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct
      661 gctttaaagt tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc
      721 aaaactcaaa taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat
      781 tcgttttttc aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca
      841 tcgaatatga gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg
      901 atggtagagg cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct
      961 cctgccaaac aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat
     1021 gcaattgttg aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac
     1081 actgatgcct tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg
     1141 cgatgcattc aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt
     1201 cctgaatttt gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt
     1261 tatccttttg tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt
     1321 catcaaccat ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt
     1381 gaaaagttcg ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga
     1441 aaagtttatg cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta
     1501 tatgcccctg ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat
     1561 gcggtgcgca cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat
     1621 ggtgagacca ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc
     1681 attgaagctt gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct
     1741 ttcagcgaaa tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt
     1801 ttaggtactg atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc
     1861 ggtgcaactt tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac
     1921 gttaccatca tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac
     1981 gtcaaagatc ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta
     2041 agcaagatga aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact
     2101 attgtttcag ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca
     2161 agagggttgc gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt
     2221 gctgcaatga cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga
     2281 gttgactctc ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct
     2341 atcaataata gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc
     2401 tcccttttgg ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag
     2461 gctgataaat tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata
     2521 actgttaaat gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga
     2581 ttgtaaaatg tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt
     2641 gctgcgacgc agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2701 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2761 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2821 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2881 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2941 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3001 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3061 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3121 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     3181 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     3241 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     3301 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattggttaa
     3361 gatccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc aaggccgaat
     3421 aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg taataatggc
     3481 ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg ctcttgatct
     3541 tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa tgcattctct
     3601 agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc atactgtttt
     3661 tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag taaagcacat
     3721 ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc taaagggcaa
     3781 aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa agcccgctta
     3841 ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc gagtttacgg
     3901 gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt aatcacttta
     3961 cttttatcta atctagacat atgaattcgg ggccgcggag gctggatcgg tcccggtgtc
     4021 ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac
     4081 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     4141 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     4201 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     4261 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     4321 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     4381 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     4441 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     4501 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     4561 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     4621 accccgtaat tgattactat taataactag tcaataatca atgtcaacat ggcggtaatg
     4681 ttggacatga gccaatataa atgtacatat tatgatatgg atacaacgta tgcaatgggc
     4741 caagctcctc gaccccccgg gccccatctc ttgccccttc taagctcgct ggactcgcac
     4801 attggctacg gtggctcgcg tcgctcgctt ggtcgctcct tcaacgtacc ctaacgttcc
     4861 ggttaacgat tcttaaagaa gcgaccaatt tgttccaccg gcttctgtgg gctcaggggc
     4921 atgtcatgga agattgagag gatcggcgct tcctccaggt atatatagag tgtccctatc
     4981 agtgatagag acttgtttca gtaagaatca attagtattc tacagtaaac atcggggccg
     5041 ctcgggcccg ctagcatgag taaaggagaa gaacttttca ctggagttgt cccaattctt
     5101 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     5161 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     5221 ccatggccaa cacttgtcac tactttgggt tatggtctaa tgtgctttgc tagataccca
     5281 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     5341 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     5401 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     5461 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     5521 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
     5581 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     5641 ccagacaacc attacctgtc ctatcaatct aagctttcga aagatcccaa cgaaaagaga
     5701 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     5761 ctatacaaaa gatcttaacg cgccacttct aaataagcga atttcttatg atttatgatt
     5821 tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag
     5881 gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct
     5941 caggtatagt atgaggtcgc tcttattgac cacacctcta ccggcagatc cgctagggat
     6001 aacagggtaa tatgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga
     6061 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
     6121 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
     6181 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cggacctgcg agcagggaaa
     6241 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
     6301 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
     6361 atacagttct cacatcacat ccgaacataa acaaccatgg gtaaaaagcc tgaactcacc
     6421 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcgg
     6481 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     6541 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     6601 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
     6661 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     6721 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     6781 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     6841 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     6901 gacaccgtcg gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     6961 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     7021 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     7081 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     7141 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     7201 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     7261 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     7321 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     7381 agtggaaacc gacgccccag cactcgtccg agggcaaagg aataatcagt actgacaata
     7441 aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc
     7501 tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgcccag
     7561 atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg
     7621 ctatactgct gtcgattcga tactaacgcc gccatccacc tgcaggtaat cacgtgccag
     7681 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     7741 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7801 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7861 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7921 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7981 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8041 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8101 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8161 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8221 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8281 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8341 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8401 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8461 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8521 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8581 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8641 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8701 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8761 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8821 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8881 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8941 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9001 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9061 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9121 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9181 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9241 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9301 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9361 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9421 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9481 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9541 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9601 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9661 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9721 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9781 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9841 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     9901 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
