LOCUS       sequence-405724-       10466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for human GATA6 
            overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Lim K, Donovan APA, Tang W, Sun D, He P, Pett JP, Teichmann SA, 
            Marioni JC, Meyer KB, Brand AH, Rawlins EL
  TITLE     Organoid modeling of human fetal lung alveolar development reveals 
            mechanisms of cell fate patterning and neonatal respiratory disease.
  JOURNAL   Cell Stem Cell. 2023 Jan 5;30(1):20-37.e9. doi: 
            10.1016/j.stem.2022.11.013. Epub 2022 Dec 8.
  PUBMED    36493780
REFERENCE   2  (bases 1 to 10466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2022.11.013"; journalName: "Cell Stem Cell"; date: 
            "2023-01-5- 5"; volume: "30"; issue: "1"; pages: "20-37.e9"
COMMENT     Sequence Label: pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G
FEATURES             Location/Qualifiers
     source          1..10466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    52..70
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    88..106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    160..178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    268..286
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     331..355
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1446..1475
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(2275..2294)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2340..2395
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2394..2413)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        2599..2810
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2823..3091
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3126..3135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3132..3842
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             3849..3902
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3903..4649
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4662..5250
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4715..4735)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5133..5144)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5325..5558
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5677..5695)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5763..5867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5868..5936,5937..6728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6086..6105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6899..7487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7388..7407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7641..7658
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7898..7919
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7934..7939,7940..7957,7958..7964)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7972..7988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7977..7999
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7996..8012
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7996..8012
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8062..8081
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8273..8906
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8953..9078
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9575..9808
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9993..10037
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10330..10447
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcagagatcc agtttggact agaccggtgg cctccacggc cactagatta ctccctatca
       61 gtgatagaga acgtatgaag agtttactcc ctatcagtga tagagaacgt atgcagactt
      121 tactccctat cagtgataga gaacgtataa ggagtttact ccctatcagt gatagagaac
      181 gtatgaccag tttactccct atcagtgata gagaacgtat ctacagttta ctccctatca
      241 gtgatagaga acgtatatcc agtttactcc ctatcagtga tagagaacgt ataagcttta
      301 ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta gtgaaccgtc agatcgcctg
      361 gagcaattcc acaacacttt tgtcttatac caactttccg taccacttcc taccctcgta
      421 aagaagttcc tattccgaag ttcctattct tcaaaaggta taggaacttc gctcgaatgg
      481 ccttgactga cggcggctgg tgcttgccga agcgcttcgg ggccgcgggt gcggacgcca
      541 gcgactccag agcctttcca gcgcgggagc cctccacgcc gccttccccc atctcttcct
      601 cgtcctcctc ctgctcccgg ggcggagagc ggggccccgg cggcgccagc aactgcggga
      661 cgcctcagct cgacacggag gcggcggccg gacccccggc ccgctcgctg ctgctcagtt
      721 cctacgcttc gcatcccttc ggggctcccc acggaccttc ggcgcctggg gtcgcgggcc
      781 ccgggggcaa cctgtcgagc tgggaggact tgctgctgtt cactgacctc gaccaagccg
      841 cgaccgccag caagctgctg tggtccagcc gcggcgccaa gctgagcccc ttcgcacccg
      901 agcagccgga ggagatgtac cagaccctcg ccgctctctc cagccagggt ccggccgcct
      961 acgacggcgc gcccggcggc ttcgtgcact ctgcggccgc ggcggcagca gccgcggcgg
     1021 cggccagctc cccggtctac gtgcccacca cccgcgtggg ttccatgctg cccggcctac
     1081 cgtaccacct gcaggggtcg ggcagtgggc cagccaacca cgcgggcggc gcgggcgcgc
     1141 accccggctg gcctcaggcc tcggccgaca gccctccata cggcagcgga ggcggcgcgg
     1201 ctggcggcgg ggccgcgggg cctggcggcg ctggctcagc cgcggcgcac gtctcggcgc
     1261 gcttccccta ctctcccagc ccgcccatgg ccaacggcgc cgcgcgggag ccgggaggct
     1321 acgcggcggc gggcagtggg ggcgcgggag gcgtgagcgg cggcggcagt agcctggcgg
     1381 ccatgggcgg ccgcgagccc cagtacagct cgctgtcggc cgcgcggccg ctgaacggga
     1441 cgtaccacca ccaccaccac caccaccacc accatccgag cccctactcg ccctacgtgg
     1501 gggcgccact gacgcctgcc tggcccgccg gacccttcga gaccccggtg ctgcacagcc
     1561 tgcagagccg cgccggagcc ccgctcccgg tgccccgggg tcccagtgca gacctgctgg
     1621 aggacctgtc cgagagccgc gagtgcgtga actgcggctc catccagacg ccgctgtggc
     1681 ggcgggacgg caccggccac tacctgtgca acgcctgcgg gctctacagc aagatgaacg
     1741 gcctcagccg gcccctcatc aagccgcaga agcgcgtgcc ttcatcacgg cggcttggat
     1801 tgtcctgtgc caactgtcac accacaacta ccaccttatg gcgcagaaac gccgagggtg
     1861 aacccgtgtg caatgcttgt ggactctaca tgaaactcca tggggtgccc agaccacttg
     1921 ctatgaaaaa agagggaatt caaaccagga aacgaaaacc taagaacata aataaatcaa
     1981 agacttgctc tggtaatagc aataattcca ttcccatgac tccaacttcc acctcttcta
     2041 actcagatga ttgcagcaaa aatacttccc ccacaacaca acctacagcc tcaggggcgg
     2101 gtgccccggt gatgactggt gcgggagaga gcaccaatcc cgagaacagc gagctcaagt
     2161 attcgggtca agatgggctc tacataggcg tcagtctcgc ctcgccggcc gaagtcacgt
     2221 cctccgtgcg accggattcc tggtgcgccc tggccctggc ctgaggccgc ctaatctttt
     2281 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
     2341 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     2401 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
     2461 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca
     2521 gtatatgaaa cagccccctg ctgtccattc cttacatatg actagtaagg atctgcgatc
     2581 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2641 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     2701 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     2761 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc
     2821 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
     2881 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct
     2941 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac
     3001 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg
     3061 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acacctgcag
     3121 ccaacgccac catggtgtct aagggcgaag agctgattaa ggagaacatg cacatgaagc
     3181 tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca
     3241 agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct
     3301 tcgccttcga catcctggct accagcttca tgtacggcag cagaaccttc atcaaccaca
     3361 cccagggcat ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagag
     3421 tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg
     3481 gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga
     3541 tgcagaagaa aacactcggc tgggaggcca acaccgagat gctgtacccc gctgacggcg
     3601 gcctggaagg cagaagcgac atggccctga agctcgtggg cgggggccac ctgatctgca
     3661 acttcaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct
     3721 actatgtgga ccacagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc
     3781 agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta
     3841 atgctagcga gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc
     3901 caatgtctag actggacaag agcaaagtca taaactctgc tctggaatta ctcaatggag
     3961 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     4021 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     4081 tggacaggca tcatacccac tcctgccccc tggaaggcga gtcatggcaa gactttctgc
     4141 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     4201 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     4261 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     4321 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     4381 cacctaccac cgattctatg cccccacttc tgaaacaagc aattgagctg ttcgaccggc
     4441 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     4501 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     4561 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     4621 attttgacct tgacatgctc cccgggtaag tcgacgatcc taatcaacct ctggattaca
     4681 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4741 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4801 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4861 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4921 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4981 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5041 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5101 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5161 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5221 gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa
     5281 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5341 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5401 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5461 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5521 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     5581 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat
     5641 taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     5701 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5761 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5821 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5881 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5941 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6001 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6061 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     6121 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6181 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6241 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6301 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6361 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6421 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6481 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6541 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6601 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6661 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6721 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6781 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6841 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6901 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6961 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7021 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7081 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7141 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7201 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7261 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7321 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7381 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7441 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7501 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7561 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7621 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7681 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact
     7741 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7801 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt
     7861 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     7921 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7981 gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat
     8041 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8101 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     8161 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
     8221 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
     8281 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     8341 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     8401 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     8461 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     8521 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     8581 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     8641 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     8701 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     8761 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8821 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8881 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     8941 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     9001 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     9061 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     9121 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     9181 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     9241 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     9301 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     9361 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     9421 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     9481 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     9541 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     9601 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     9661 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     9721 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     9781 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     9841 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     9901 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     9961 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
    10021 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
    10081 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
    10141 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
    10201 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
    10261 gatccattcg attagtgaac ggatctcgac ggtatcgccg aattcacaaa tggcagtatt
    10321 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
    10381 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
    10441 aaattttcgg gtttattaca gggaca
//
