LOCUS       sequence-405725-        9794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) 
            overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Lim K, Donovan APA, Tang W, Sun D, He P, Pett JP, Teichmann SA, 
            Marioni JC, Meyer KB, Brand AH, Rawlins EL
  TITLE     Organoid modeling of human fetal lung alveolar development reveals 
            mechanisms of cell fate patterning and neonatal respiratory disease.
  JOURNAL   Cell Stem Cell. 2023 Jan 5;30(1):20-37.e9. doi: 
            10.1016/j.stem.2022.11.013. Epub 2022 Dec 8.
  PUBMED    36493780
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2022.11.013"; journalName: "Cell Stem Cell"; date: 
            "2023-01-5- 5"; volume: "30"; issue: "1"; pages: "20-37.e9"
COMMENT     Sequence Label: pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G
FEATURES             Location/Qualifiers
     source          1..9794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             52..685
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    732..857
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1354..1587
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1772..1816
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2109..2226
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2297..2315
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2333..2351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2369..2387
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2405..2423
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2441..2459
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2477..2495
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2513..2531
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2576..2600
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(3848..3867)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3913..3968
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3967..3986)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        4172..4383
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4396..4664
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4699..4708
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4705..5415
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5422..5475
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5476..6222
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6235..6823
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6288..6308)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6706..6717)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6898..7131
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7250..7268)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7336..7440
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7441..7509,7510..8301)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7659..7678)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8472..9060
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8961..8980
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9214..9231
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9471..9492
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9507..9512,9513..9530,9531..9537)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9545..9561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9550..9572
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9569..9585
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9569..9585
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9635..9654
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
       61 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
      121 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
      181 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
      241 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
      301 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
      361 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
      421 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
      481 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
      541 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
      601 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
      661 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
      721 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
      781 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
      841 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
      901 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
      961 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1021 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1081 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1141 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1201 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     1261 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     1321 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1381 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1441 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1501 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1561 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     1621 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     1681 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     1741 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     1801 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     1861 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     1921 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     1981 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2041 atccattcga ttagtgaacg gatctcgacg gtatcgccga attcacaaat ggcagtattc
     2101 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2161 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2221 aattttcggg tttattacag ggacagcaga gatccagttt ggactagacc ggtggcctcc
     2281 acggccacta gattactccc tatcagtgat agagaacgta tgaagagttt actccctatc
     2341 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
     2401 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
     2461 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
     2521 agtgatagag aacgtataag ctttaggcgt gtacggtggg cgcctataaa agcagagctc
     2581 gtttagtgaa ccgtcagatc gcctggagca attccacaac acttttgtct tataccaact
     2641 ttccgtacca cttcctaccc tcgtaaagaa gttcctattc cgaagttcct attcttcaaa
     2701 aggtatagga acttcgctcg aatgtcgatg agtccaaagc acacgactcc gttctcagtg
     2761 tctgacatct tgagtcccct ggaggaaagc tacaagaaag tgggcatgga gggcggcggc
     2821 ctcggggctc cgctggcggc gtacaggcag ggccaggcgg caccgccaac agcggccatg
     2881 cagcagcacg ccgtggggca ccacggcgcc gtcaccgccg cctaccacat gacggcggcg
     2941 ggggtgcccc agctctcgca ctccgccgtg gggggctact gcaacggcaa cctgggcaac
     3001 atgagcgagc tgccgccgta ccaggacacc atgaggaaca gcgcctctgg ccccggatgg
     3061 tacggcgcca acccagaccc gcgcttcccc gccatctccc gcttcatggg cccggcgagc
     3121 ggcatgaaca tgagcggcat gggcggcctg ggctcgctgg gggacgtgag caagaacatg
     3181 gccccgctgc caagcgcgcc gcgcaggaag cgccgggtgc tcttctcgca ggcgcaggtg
     3241 tacgagctgg agcgacgctt caagcaacag aagtacctgt cggcgccgga gcgcgagcac
     3301 ctggccagca tgatccacct gacgcccacg caggtcaagt tctggttcca gaaccaccgc
     3361 tacaaaatga agcgccaggc caaggacaag gcggcgcagc agcaactgca gcaggacagc
     3421 ggcggcggcg ggggcggcgg gggcaccggg tgcccgcagc agcaacaggc tcagcagcag
     3481 tcgccgcgac gcgtggcggt gccggtcctg gtgaaagacg gcaaaccgtg ccaggcgggt
     3541 gcccccgcgc cgggcgccgc cagcctacaa ggccacgcgc agcagcaggc gcagcaccag
     3601 gcgcaggccg cgcaggcggc ggcagcggcc atctccgtgg gcagcggtgg cgccggcctt
     3661 ggcgcacacc cgggccacca gccaggcagc gcaggccagt ctccggacct ggcgcaccac
     3721 gccgccagcc ccgcggcgct gcagggccag gtatccagcc tgtcccacct gaactcctcg
     3781 ggctcggact acggcaccat gtcctgctcc accttgctat acggtcggac ctggtgaggc
     3841 cgcctaatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     3901 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     3961 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     4021 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     4081 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttacat atgactagta
     4141 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4201 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     4261 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4321 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4381 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     4441 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     4501 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     4561 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     4621 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     4681 cctacacctg cagccaacgc caccatggtg tctaagggcg aagagctgat taaggagaac
     4741 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc
     4801 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     4861 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc
     4921 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc
     4981 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     5041 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc
     5101 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga gatgctgtac
     5161 cccgctgacg gcggcctgga aggcagaagc gacatggccc tgaagctcgt gggcgggggc
     5221 cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag
     5281 atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag
     5341 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
     5401 gggcacaaac ttaatgctag cgagggcaga ggaagtctgc taacatgcgg tgacgtcgag
     5461 gagaatcctg gcccaatgtc tagactggac aagagcaaag tcataaactc tgctctggaa
     5521 ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga
     5581 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg
     5641 ccaatcgaga tgctggacag gcatcatacc cactcctgcc ccctggaagg cgagtcatgg
     5701 caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac
     5761 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
     5821 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
     5881 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa
     5941 gaggaaagag agacacctac caccgattct atgcccccac ttctgaaaca agcaattgag
     6001 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
     6061 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt
     6121 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
     6181 gacgctcttg acgattttga ccttgacatg ctccccgggt aagtcgacga tcctaatcaa
     6241 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6301 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6361 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6421 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6481 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6541 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6601 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6661 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6721 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6781 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac
     6841 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6901 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     6961 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7021 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7081 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7141 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7201 ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     7261 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     7321 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7381 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7441 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7501 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7561 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7621 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7681 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7741 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7801 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7861 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7921 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7981 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8041 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8101 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8161 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8221 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8281 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8341 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8401 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8461 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8521 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8581 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     8641 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8701 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8761 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8821 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8881 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8941 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9001 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9061 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     9121 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     9181 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     9241 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
     9301 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     9361 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
     9421 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     9481 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9541 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
     9601 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9661 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     9721 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     9781 tcctctgaat tatt
//
