LOCUS       sequence-405726-       10031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for human TFAP2C 
            overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Lim K, Donovan APA, Tang W, Sun D, He P, Pett JP, Teichmann SA, 
            Marioni JC, Meyer KB, Brand AH, Rawlins EL
  TITLE     Organoid modeling of human fetal lung alveolar development reveals 
            mechanisms of cell fate patterning and neonatal respiratory disease.
  JOURNAL   Cell Stem Cell. 2023 Jan 5;30(1):20-37.e9. doi: 
            10.1016/j.stem.2022.11.013. Epub 2022 Dec 8.
  PUBMED    36493780
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2022.11.013"; journalName: "Cell Stem Cell"; date: 
            "2023-01-5- 5"; volume: "30"; issue: "1"; pages: "20-37.e9"
COMMENT     Sequence Label: pLenti-tetON-TFAP2C-EF1a-TagRFP-2A-tet3G
FEATURES             Location/Qualifiers
     source          1..10031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    357..378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(393..398,399..416,417..423)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    431..447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     436..458
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     455..471
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     455..471
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     521..540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             732..1365
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1412..1537
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2034..2267
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2452..2496
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2789..2906
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2977..2995
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3013..3031
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3049..3067
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3085..3103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3121..3139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3157..3175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3193..3211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3256..3280
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(4765..4784)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4830..4885
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4884..4903)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        5089..5300
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5313..5581
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5616..5625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5622..6332
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             6339..6392
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6393..7139
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7152..7740
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7205..7225)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7623..7634)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7815..8048
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8167..8185)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8253..8357
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8358..8426,8427..9218)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8576..8595)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9389..9977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9878..9897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
       61 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      181 agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
      241 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
      301 tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      361 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
      421 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg
      481 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
      541 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
      601 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
      661 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
      721 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
      781 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
      841 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
      901 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
      961 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     1021 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     1081 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     1141 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     1201 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     1261 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     1321 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     1381 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     1441 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     1501 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     1561 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     1621 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     1681 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     1741 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1801 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1861 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1921 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1981 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     2041 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     2101 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     2161 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     2221 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     2281 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     2341 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2401 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2461 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2521 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2581 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2641 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2701 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccga
     2761 attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     2821 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     2881 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
     2941 ggactagacc ggtggcctcc acggccacta gattactccc tatcagtgat agagaacgta
     3001 tgaagagttt actccctatc agtgatagag aacgtatgca gactttactc cctatcagtg
     3061 atagagaacg tataaggagt ttactcccta tcagtgatag agaacgtatg accagtttac
     3121 tccctatcag tgatagagaa cgtatctaca gtttactccc tatcagtgat agagaacgta
     3181 tatccagttt actccctatc agtgatagag aacgtataag ctttaggcgt gtacggtggg
     3241 cgcctataaa agcagagctc gtttagtgaa ccgtcagatc gcctggagca attccacaac
     3301 acttttgtct tataccaact ttccgtacca cttcctaccc tcgtaaagaa gttcctattc
     3361 cgaagttcct attcttcaaa aggtatagga acttcgctcg aatgttgtgg aaaataaccg
     3421 ataatgtcaa gtacgaagag gactgcgagg atcgccacga cgggagcagc aatgggaatc
     3481 cgcgggtccc ccacctctcc tccgccgggc agcacctcta cagccccgcg ccacccctct
     3541 cccacactgg agtcgccgaa tatcagccgc caccctactt tccccctccc taccagcagc
     3601 tggcctactc ccagtcggcc gacccctact cgcatctggg ggaagcgtac gccgccgcca
     3661 tcaaccccct gcaccagccg gcgcccacag gcagccagca gcaggcctgg cccggccgcc
     3721 agagccagga gggagcgggg ctgccctcgc accacgggcg cccggccggc ctactgcccc
     3781 acctctccgg gctggaggcg ggcgcggtga gcgcccgcag ggatgcctac cgccgctccg
     3841 acctgctgct gccccacgca cacgccctgg atgccgcggg cctggccgag aacctggggc
     3901 tccacgacat gcctcaccag atggacgagg tgcagaatgt cgacgaccag cacctgttgc
     3961 tgcacgatca gacagtcatt cgcaaaggtc ccatttccat gaccaagaac cctctgaacc
     4021 tcccctgtca gaaggagctg gtgggggccg taatgaaccc cactgaggtc ttctgctcag
     4081 tccctggaag attgtcgctc ctcagctcta cgtctaaata caaagtgaca gtggctgaag
     4141 tacagaggcg actgtcccca cctgaatgct taaatgcctc gttactggga ggtgttctca
     4201 gaagagccaa atcgaaaaat ggaggccggt ccttgcggga gaagttggac aagattgggt
     4261 tgaatcttcc ggccgggagg cggaaagccg ctcatgtgac tctcctgaca tccttagtag
     4321 aaggtgaagc tgttcatttg gctagggact ttgcctatgt ctgtgaagcc gaatttccta
     4381 gtaaaccagt ggcagaatat ttaaccagac ctcatcttgg aggacgaaat gagatggcag
     4441 ctaggaagaa catgctattg gcggcccagc aactgtgtaa agaattcaca gaacttctca
     4501 gccaagaccg gacaccccat gggaccagca ggctcgcccc agtcttggag acgaacatac
     4561 agaactgctt gtctcatttc agcctgatta cccacgggtt tggcagccag gccatctgtg
     4621 ccgcggtgtc tgccctgcag aactacatca aagaagccct gattgtcata gacaaatcct
     4681 acatgaaccc tggagaccag agtccagctg attctaacaa aaccctggag aaaatggaga
     4741 aacacaggaa ataaggccgc ctaatctttt tccctctgcc aaaaattatg gggacatcat
     4801 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     4861 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     4921 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     4981 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     5041 cttacatatg actagtaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
     5101 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
     5161 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     5221 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     5281 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
     5341 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
     5401 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
     5461 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
     5521 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
     5581 ccaagctgtg accggcgcct acacctgcag ccaacgccac catggtgtct aagggcgaag
     5641 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc
     5701 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
     5761 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca
     5821 tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt
     5881 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
     5941 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     6001 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcca
     6061 acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac atggccctga
     6121 agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga tccaagaaac
     6181 ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg gaaagaatca
     6241 aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg
     6301 acctccctag caaactgggg cacaaactta atgctagcga gggcagagga agtctgctaa
     6361 catgcggtga cgtcgaggag aatcctggcc caatgtctag actggacaag agcaaagtca
     6421 taaactctgc tctggaatta ctcaatggag tcggtatcga aggcctgacg acaaggaaac
     6481 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
     6541 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac tcctgccccc
     6601 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc
     6661 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
     6721 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
     6781 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
     6841 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
     6901 tgaaacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
     6961 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg
     7021 acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc
     7081 ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaag
     7141 tcgacgatcc taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7201 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7261 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7321 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7381 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7441 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7501 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7561 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7621 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7681 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7741 ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7801 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     7861 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7921 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7981 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8041 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     8101 aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac
     8161 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     8221 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8281 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8341 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8401 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8461 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8521 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8581 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     8641 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8701 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8761 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8821 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8881 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8941 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9001 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9061 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9121 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9181 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9241 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9301 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9361 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9421 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9481 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9541 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9601 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9661 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9721 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9781 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9841 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9901 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9961 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10021 ggccttttgc t
//
