LOCUS       sequence-405727-        9794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) 
            overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Lim K, Donovan APA, Tang W, Sun D, He P, Pett JP, Teichmann SA, 
            Marioni JC, Meyer KB, Brand AH, Rawlins EL
  TITLE     Organoid modeling of human fetal lung alveolar development reveals 
            mechanisms of cell fate patterning and neonatal respiratory disease.
  JOURNAL   Cell Stem Cell. 2023 Jan 5;30(1):20-37.e9. doi: 
            10.1016/j.stem.2022.11.013. Epub 2022 Dec 8.
  PUBMED    36493780
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2022.11.013"; journalName: "Cell Stem Cell"; date: 
            "2023-01-5- 5"; volume: "30"; issue: "1"; pages: "20-37.e9"
COMMENT     Sequence Label: pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G
FEATURES             Location/Qualifiers
     source          1..9794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..137
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    634..867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1052..1096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1389..1506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1577..1595
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1613..1631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1649..1667
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1685..1703
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1721..1739
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1757..1775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1793..1811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1856..1880
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(3128..3147)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3193..3248
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3247..3266)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        3452..3663
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3676..3944
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3979..3988
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3985..4695
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             4702..4755
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4756..5502
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5515..6103
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5568..5588)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5986..5997)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6178..6411
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6530..6548)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6616..6720
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6721..6789,6790..7581)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6939..6958)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7752..8340
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8241..8260
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8494..8511
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8751..8772
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8787..8792,8793..8810,8811..8817)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8825..8841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8830..8852
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8849..8865
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8849..8865
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8915..8934
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9126..9759
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
       61 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
      121 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
      181 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
      241 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
      301 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
      361 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
      421 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
      481 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
      541 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
      601 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
      661 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
      721 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
      781 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
      841 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
      901 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
      961 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     1021 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     1081 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     1141 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     1201 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     1261 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     1321 atccattcga ttagtgaacg gatctcgacg gtatcgccga attcacaaat ggcagtattc
     1381 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1441 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1501 aattttcggg tttattacag ggacagcaga gatccagttt ggactagacc ggtggcctcc
     1561 acggccacta gattactccc tatcagtgat agagaacgta tgaagagttt actccctatc
     1621 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
     1681 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
     1741 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
     1801 agtgatagag aacgtataag ctttaggcgt gtacggtggg cgcctataaa agcagagctc
     1861 gtttagtgaa ccgtcagatc gcctggagca attccacaac acttttgtct tataccaact
     1921 ttccgtacca cttcctaccc tcgtaaagaa gttcctattc cgaagttcct attcttcaaa
     1981 aggtatagga acttcgctcg aatgtcgatg agtccaaagc acacgactcc gttctcagtg
     2041 tctgacatct tgagtcccct ggaggaaagc tacaagaaag tgggcatgga gggcggcggc
     2101 ctcggggctc cgctggcggc gtacaggcag ggccaggcgg caccgccaac agcggccatg
     2161 cagcagcacg ccgtggggca ccacggcgcc gtcaccgccg cctaccacat gacggcggcg
     2221 ggggtgcccc agctctcgca ctccgccgtg gggggctact gcaacggcaa cctgggcaac
     2281 atgagcgagc tgccgccgta ccaggacacc atgaggaaca gcgcctctgg ccccggatgg
     2341 tacggcgcca acccagaccc gcgcttcccc gccatctccc gcttcatggg cccggcgagc
     2401 ggcatgaaca tgagcggcat gggcggcctg ggctcgctgg gggacgtgag caagaacatg
     2461 gccccgctgc caagcgcgcc gcgcaggaag cgccgggtgc tcttctcgca ggcgcaggtg
     2521 tacgagctgg agtgacgctt caagcaacag aagtacctgt cggcgccgga gcgcgagcac
     2581 ctggccagca tgatccacct gacgcccacg caggtcaaga tctggttcca gaaccaccgc
     2641 tacaaaatga agcgccaggc caaggacaag gcggcgcagc agcaactgca gcaggacagc
     2701 ggcggcggcg ggggcggcgg gggcaccggg tgcccgcagc agcaacaggc tcagcagcag
     2761 tcgccgcgac gcgtggcggt gccggtcctg gtgaaagacg gcaaaccgtg ccaggcgggt
     2821 gcccccgcgc cgggcgccgc cagcctacaa ggccacgcgc agcagcaggc gcagcaccag
     2881 gcgcaggccg cgcaggcggc ggcagcggcc atctccgtgg gcagcggtgg cgccggcctt
     2941 ggcgcacacc cgggccacca gccaggcagc gcaggccagt ctccggacct ggcgcaccac
     3001 gccgccagcc ccgcggcgct gcagggccag gtatccagcc tgtcccacct gaactcctcg
     3061 ggctcggact acggcaccat gtcctgctcc accttgctat acggtcggac ctggtgaggc
     3121 cgcctaatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     3181 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     3241 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     3301 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     3361 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttacat atgactagta
     3421 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     3481 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     3541 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     3601 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     3661 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     3721 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     3781 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     3841 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     3901 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     3961 cctacacctg cagccaacgc caccatggtg tctaagggcg aagagctgat taaggagaac
     4021 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc
     4081 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     4141 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc
     4201 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc
     4261 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     4321 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc
     4381 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga gatgctgtac
     4441 cccgctgacg gcggcctgga aggcagaagc gacatggccc tgaagctcgt gggcgggggc
     4501 cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag
     4561 atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag
     4621 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
     4681 gggcacaaac ttaatgctag cgagggcaga ggaagtctgc taacatgcgg tgacgtcgag
     4741 gagaatcctg gcccaatgtc tagactggac aagagcaaag tcataaactc tgctctggaa
     4801 ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga
     4861 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg
     4921 ccaatcgaga tgctggacag gcatcatacc cactcctgcc ccctggaagg cgagtcatgg
     4981 caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac
     5041 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
     5101 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
     5161 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa
     5221 gaggaaagag agacacctac caccgattct atgcccccac ttctgaaaca agcaattgag
     5281 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
     5341 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt
     5401 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
     5461 gacgctcttg acgattttga ccttgacatg ctccccgggt aagtcgacga tcctaatcaa
     5521 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5581 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5641 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5701 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5761 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5821 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     5881 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     5941 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6001 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6061 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac
     6121 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6181 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     6241 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6301 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6361 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     6421 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6481 ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     6541 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     6601 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6661 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6721 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6781 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6841 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6901 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6961 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7021 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7081 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7141 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7201 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7261 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7321 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7381 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7441 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7501 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7561 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7621 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7681 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7741 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7801 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7861 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     7921 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7981 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8041 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8101 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8161 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8221 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8281 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8341 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8401 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8461 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8521 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
     8581 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8641 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
     8701 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8761 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     8821 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
     8881 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8941 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     9001 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     9061 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     9121 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     9181 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     9241 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     9301 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     9361 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     9421 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     9481 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     9541 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     9601 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     9661 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9721 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     9781 gaaagcgaaa ggga
//
