LOCUS       sequence-405728-        9794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) 
            overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Lim K, Donovan APA, Tang W, Sun D, He P, Pett JP, Teichmann SA, 
            Marioni JC, Meyer KB, Brand AH, Rawlins EL
  TITLE     Organoid modeling of human fetal lung alveolar development reveals 
            mechanisms of cell fate patterning and neonatal respiratory disease.
  JOURNAL   Cell Stem Cell. 2023 Jan 5;30(1):20-37.e9. doi: 
            10.1016/j.stem.2022.11.013. Epub 2022 Dec 8.
  PUBMED    36493780
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2022.11.013"; journalName: "Cell Stem Cell"; date: 
            "2023-01-5- 5"; volume: "30"; issue: "1"; pages: "20-37.e9"
COMMENT     Sequence Label: pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G
FEATURES             Location/Qualifiers
     source          1..9794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(74..93)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      887..1475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1376..1395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1629..1646
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1886..1907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1922..1927,1928..1945,1946..1952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1960..1976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1965..1987
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1984..2000
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1984..2000
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2050..2069
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2261..2894
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2941..3066
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3563..3796
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3981..4025
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4318..4435
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4506..4524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4542..4560
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4578..4596
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4614..4632
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4650..4668
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4686..4704
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4722..4740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4785..4809
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(6057..6076)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6122..6177
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6176..6195)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        6381..6592
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6605..6873
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      6908..6917
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6914..7624
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             7631..7684
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7685..8431
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8444..9032
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8497..8517)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8915..8926)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9107..9340
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9459..9477)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9545..9649
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9650..9718,9719..9794,1..716)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
       61 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
      121 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      181 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      241 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
      301 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
      361 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
      421 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
      481 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
      541 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
      601 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
      661 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
      721 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
      781 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
      841 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
      901 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
      961 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1021 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1081 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1141 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1201 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1261 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1321 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     1381 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     1441 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     1501 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     1561 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     1621 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     1681 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat
     1741 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     1801 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga
     1861 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     1921 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     1981 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc
     2041 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     2101 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     2161 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
     2221 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
     2281 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     2341 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     2401 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     2461 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     2521 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     2581 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     2641 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
     2701 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     2761 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     2821 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     2881 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     2941 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     3001 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     3061 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     3121 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     3181 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     3241 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     3301 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     3361 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct
     3421 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     3481 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     3541 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     3601 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     3661 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     3721 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     3781 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     3841 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     3901 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     3961 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     4021 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     4081 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     4141 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     4201 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     4261 tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg gcagtattca tccacaattt
     4321 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4381 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     4441 ttattacagg gacagcagag atccagtttg gactagaccg gtggcctcca cggccactag
     4501 attactccct atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga
     4561 acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat
     4621 cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag
     4681 tttactccct atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga
     4741 acgtataagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg tttagtgaac
     4801 cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
     4861 ttcctaccct cgtaaagaag ttcctattcc gaagttccta ttcttcaaaa ggtataggaa
     4921 cttcgctcga atgtcgatga gtccaaagca cacgactccg ttctcagtgt ctgacatctt
     4981 gagtcccctg gaggaaagct acaagaaagt gggcatggag ggcggcggcc tcggggctcc
     5041 gctggcggcg tacaggcagg gccaggcggc accgccaaca gcggccatgc agcagcacgc
     5101 cgtggggcac cacggcgccg tcaccgccgc ctaccacatg acggcggcgg gggtgcccca
     5161 gctctcgcac tccgccgtgg ggggctactg caacggcaac ctgggcaaca tgagcgagct
     5221 gccgccgtac caggacacca tgaggaacag cgcctctggc cccggatggt acggcgccaa
     5281 cccagacccg cgcttccccg ccatctcccg cttcatgggc ccggcgagcg gcatgaacat
     5341 gagcggcatg ggcggcctgg gctcgctggg ggacgtgagc aagaacatgg ccccgctgcc
     5401 aagcgcgccg cgcaggaagc gccgggtgct cttctcgcag gcgcaggtgt actagctgga
     5461 gcgacgcttc aagcaacaga agtacctgtc ggcgccggag cgcgagcacc tggccagcat
     5521 gatccacctg acgcccacgc aggtcaagat ctggttccag aaccaccgct acaaaatgaa
     5581 gcgccaggcc aaggacaagg cggcgcagca gcaactgcag caggacagcg gcggcggcgg
     5641 gggcggcggg ggcaccgggt gcccgcagca gcaacaggct cagcagcagt cgccgcgacg
     5701 cgtggcggtg ccggtcctgg tgaaagacgg caaaccgtgc caggcgggtg cccccgcgcc
     5761 gggcgccgcc agcctacaag gccacgcgca gcagcaggcg cagcaccagg cgcaggccgc
     5821 gcaggcggcg gcagcggcca tctccgtggg cagcggtggc gccggccttg gcgcacaccc
     5881 gggccaccag ccaggcagcg caggccagtc tccggacctg gcgcaccacg ccgccagccc
     5941 cgcggcgctg cagggccagg tatccagcct gtcccacctg aactcctcgg gctcggacta
     6001 cggcaccatg tcctgctcca ccttgctata cggtcggacc tggtgaggcc gcctaatctt
     6061 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     6121 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     6181 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     6241 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     6301 cagtatatga aacagccccc tgctgtccat tccttacata tgactagtaa ggatctgcga
     6361 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     6421 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     6481 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     6541 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     6601 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     6661 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     6721 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     6781 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     6841 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacacctgc
     6901 agccaacgcc accatggtgt ctaagggcga agagctgatt aaggagaaca tgcacatgaa
     6961 gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg
     7021 caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc
     7081 cttcgccttc gacatcctgg ctaccagctt catgtacggc agcagaacct tcatcaacca
     7141 cacccagggc atccccgact tctttaagca gtccttccct gagggcttca catgggagag
     7201 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga
     7261 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt
     7321 gatgcagaag aaaacactcg gctgggaggc caacaccgag atgctgtacc ccgctgacgg
     7381 cggcctggaa ggcagaagcg acatggccct gaagctcgtg ggcgggggcc acctgatctg
     7441 caacttcaag accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt
     7501 ctactatgtg gaccacagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga
     7561 gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacaaact
     7621 taatgctagc gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg
     7681 cccaatgtct agactggaca agagcaaagt cataaactct gctctggaat tactcaatgg
     7741 agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     7801 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     7861 gctggacagg catcataccc actcctgccc cctggaaggc gagtcatggc aagactttct
     7921 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     7981 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     8041 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     8101 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     8161 gacacctacc accgattcta tgcccccact tctgaaacaa gcaattgagc tgttcgaccg
     8221 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     8281 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     8341 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     8401 cgattttgac cttgacatgc tccccgggta agtcgacgat cctaatcaac ctctggatta
     8461 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     8521 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     8581 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     8641 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     8701 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     8761 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     8821 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     8881 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     8941 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     9001 gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac
     9061 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     9121 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     9181 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     9241 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9301 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     9361 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta
     9421 attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt
     9481 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     9541 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     9601 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     9661 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     9721 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     9781 catcgaactg gatc
//
