LOCUS       sequence-405732-        9794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) 
            overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Lim K, Donovan APA, Tang W, Sun D, He P, Pett JP, Teichmann SA, 
            Marioni JC, Meyer KB, Brand AH, Rawlins EL
  TITLE     Organoid modeling of human fetal lung alveolar development reveals 
            mechanisms of cell fate patterning and neonatal respiratory disease.
  JOURNAL   Cell Stem Cell. 2023 Jan 5;30(1):20-37.e9. doi: 
            10.1016/j.stem.2022.11.013. Epub 2022 Dec 8.
  PUBMED    36493780
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2022.11.013"; journalName: "Cell Stem Cell"; date: 
            "2023-01-5- 5"; volume: "30"; issue: "1"; pages: "20-37.e9"
COMMENT     Sequence Label: pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G
FEATURES             Location/Qualifiers
     source          1..9794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(682..701)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    747..802
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(801..820)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        1006..1217
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1230..1498
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      1533..1542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1539..2249
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             2256..2309
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2310..3056
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3069..3657
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3122..3142)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3540..3551)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3732..3965
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4084..4102)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4170..4274
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4275..4343,4344..5135)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4493..4512)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5306..5894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5795..5814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6048..6065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6305..6326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6341..6346,6347..6364,6365..6371)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6379..6395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6384..6406
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6403..6419
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6403..6419
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6469..6488
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6680..7313
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7360..7485
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7982..8215
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8400..8444
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8737..8854
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    8925..8943
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8961..8979
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8997..9015
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9033..9051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9069..9087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9105..9123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9141..9159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     9204..9228
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tgagcaagaa catggccccg ctgccaagcg cgccgcgcag gaagcgccgg gtgctcttct
       61 cgcaggcgca ggtgtacgag ctggagcgac gcttcaagca acagaagtac ctgtcggcgc
      121 cggagcgcga gcacctggcc agcatgatcc acctgacgcc cacgcaggtc aagatgtggt
      181 tccagaacca ccgctacaaa atgaagcgcc aggccaagga caaggcggcg cagcagcaac
      241 tgcagcagga cagcggcggc ggcgggggcg gcgggggcac cgggtgcccg cagcagcaac
      301 aggctcagca gcagtcgccg cgacgcgtgg cggtgccggt cctggtgaaa gacggcaaac
      361 cgtgccaggc gggtgccccc gcgccgggcg ccgccagcct acaaggccac gcgcagcagc
      421 aggcgcagca ccaggcgcag gccgcgcagg cggcggcagc ggccatctcc gtgggcagcg
      481 gtggcgccgg ccttggcgca cacccgggcc accagccagg cagcgcaggc cagtctccgg
      541 acctggcgca ccacgccgcc agccccgcgg cgctgcaggg ccaggtatcc agcctgtccc
      601 acctgaactc ctcgggctcg gactacggca ccatgtcctg ctccaccttg ctatacggtc
      661 ggacctggtg aggccgccta atctttttcc ctctgccaaa aattatgggg acatcatgaa
      721 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
      781 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
      841 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
      901 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
      961 acatatgact agtaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat
     1021 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa
     1081 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     1141 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     1201 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc
     1261 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     1321 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc
     1381 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     1441 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca
     1501 agctgtgacc ggcgcctaca cctgcagcca acgccaccat ggtgtctaag ggcgaagagc
     1561 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
     1621 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
     1681 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
     1741 acggcagcag aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct
     1801 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     1861 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     1921 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccaaca
     1981 ccgagatgct gtaccccgct gacggcggcc tggaaggcag aagcgacatg gccctgaagc
     2041 tcgtgggcgg gggccacctg atctgcaact tcaagaccac atacagatcc aagaaacccg
     2101 ctaagaacct caagatgccc ggcgtctact atgtggacca cagactggaa agaatcaagg
     2161 aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
     2221 tccctagcaa actggggcac aaacttaatg ctagcgaggg cagaggaagt ctgctaacat
     2281 gcggtgacgt cgaggagaat cctggcccaa tgtctagact ggacaagagc aaagtcataa
     2341 actctgctct ggaattactc aatggagtcg gtatcgaagg cctgacgaca aggaaactcg
     2401 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
     2461 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccactcc tgccccctgg
     2521 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc
     2581 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
     2641 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
     2701 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
     2761 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
     2821 aacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
     2881 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg
     2941 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
     3001 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaagtcg
     3061 acgatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3121 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3181 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3241 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3301 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3361 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3421 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     3481 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3541 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3601 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     3661 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     3721 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     3781 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     3841 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     3901 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     3961 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     4021 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa
     4081 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4141 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4201 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4261 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4321 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4381 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4441 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4501 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4561 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4621 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4681 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4741 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4801 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4861 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4921 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4981 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5041 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5101 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5161 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5221 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5281 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5341 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5401 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5461 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5521 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5581 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5641 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5701 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5761 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5821 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5881 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5941 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6001 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6061 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6121 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     6241 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6301 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6361 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
     6421 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6481 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
     6541 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
     6601 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
     6661 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
     6721 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     6781 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     6841 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     6901 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     6961 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     7021 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     7081 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     7141 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7201 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7261 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     7321 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     7381 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     7441 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     7501 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     7561 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     7621 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     7681 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     7741 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     7801 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
     7861 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     7921 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     7981 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     8041 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     8101 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     8161 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     8221 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     8281 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     8341 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     8401 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     8461 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     8521 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     8581 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     8641 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
     8701 atcgccgaat tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     8761 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     8821 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     8881 tccagtttgg actagaccgg tggcctccac ggccactaga ttactcccta tcagtgatag
     8941 agaacgtatg aagagtttac tccctatcag tgatagagaa cgtatgcaga ctttactccc
     9001 tatcagtgat agagaacgta taaggagttt actccctatc agtgatagag aacgtatgac
     9061 cagtttactc cctatcagtg atagagaacg tatctacagt ttactcccta tcagtgatag
     9121 agaacgtata tccagtttac tccctatcag tgatagagaa cgtataagct ttaggcgtgt
     9181 acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc ctggagcaat
     9241 tccacaacac ttttgtctta taccaacttt ccgtaccact tcctaccctc gtaaagaagt
     9301 tcctattccg aagttcctat tcttcaaaag gtataggaac ttcgctcgaa tgtcgatgag
     9361 tccaaagcac acgactccgt tctcagtgtc tgacatcttg agtcccctgg aggaaagcta
     9421 caagaaagtg ggcatggagg gcggcggcct cggggctccg ctggcggcgt acaggcaggg
     9481 ccaggcggca ccgccaacag cggccatgca gcagcacgcc gtggggcacc acggcgccgt
     9541 caccgccgcc taccacatga cggcggcggg ggtgccccag ctctcgcact ccgccgtggg
     9601 gggctactgc aacggcaacc tgggcaacat gagcgagctg ccgccgtacc aggacaccat
     9661 gaggaacagc gcctctggcc ccggatggta cggcgccaac ccagacccgc gcttccccgc
     9721 catctcccgc ttcatgggcc cggcgagcgg catgaacatg agcggcatgg gcggcctggg
     9781 ctcgctgggg gacg
//
