LOCUS       sequence-405735-        9791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) 
            overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Lim K, Donovan APA, Tang W, Sun D, He P, Pett JP, Teichmann SA, 
            Marioni JC, Meyer KB, Brand AH, Rawlins EL
  TITLE     Organoid modeling of human fetal lung alveolar development reveals 
            mechanisms of cell fate patterning and neonatal respiratory disease.
  JOURNAL   Cell Stem Cell. 2023 Jan 5;30(1):20-37.e9. doi: 
            10.1016/j.stem.2022.11.013. Epub 2022 Dec 8.
  PUBMED    36493780
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2022.11.013"; journalName: "Cell Stem Cell"; date: 
            "2023-01-5- 5"; volume: "30"; issue: "1"; pages: "20-37.e9"
COMMENT     Sequence Label: pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G
FEATURES             Location/Qualifiers
     source          1..9791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..156
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    263..281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    299..317
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    335..353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    371..389
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    407..425
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    443..461
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     506..530
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(1775..1794)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1840..1895
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1894..1913)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        2099..2310
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2323..2591
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2626..2635
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2632..3342
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             3349..3402
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3403..4149
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4162..4750
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4215..4235)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4633..4644)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4825..5058
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5177..5195)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5263..5367
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5368..5436,5437..6228)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5586..5605)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6399..6987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6888..6907
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7141..7158
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7398..7419
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7434..7439,7440..7457,7458..7464)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7472..7488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7477..7499
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7496..7512
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7496..7512
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7562..7581
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7773..8406
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8453..8578
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9075..9308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9493..9537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtatcgccga attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
       61 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
      121 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
      181 gatccagttt ggactagacc ggtggcctcc acggccacta gattactccc tatcagtgat
      241 agagaacgta tgaagagttt actccctatc agtgatagag aacgtatgca gactttactc
      301 cctatcagtg atagagaacg tataaggagt ttactcccta tcagtgatag agaacgtatg
      361 accagtttac tccctatcag tgatagagaa cgtatctaca gtttactccc tatcagtgat
      421 agagaacgta tatccagttt actccctatc agtgatagag aacgtataag ctttaggcgt
      481 gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc gcctggagca
      541 attccacaac acttttgtct tataccaact ttccgtacca cttcctaccc tcgtaaagaa
      601 gttcctattc cgaagttcct attcttcaaa aggtatagga acttcgctcg aatgtcgatg
      661 agtccaaagc acacgactcc gttctcagtg tctgacatct tgagtcccct ggaggaaagc
      721 tacaagaaag tgggcatgga gggcggcggc ctcggggctc cgctggcggc gtacaggcag
      781 ggccaggcgg caccgccaac agcggccatg cagcagcacg ccgtggggca ccacggcgcc
      841 gtcaccgccg cctaccacat gacggcggcg ggggtgcccc agctctcgca ctccgccgtg
      901 gggggctact gcaacggcaa cctgggcaac atgagcgagc tgccgccgta ccaggacacc
      961 atgaggaaca gcgcctctgg ccccggatgg tacggcgcca acccagaccc gcgcttcccc
     1021 gccatctccc gcttcatggg cccggcgagc ggcatgaaca tgagcggcat gggcggcctg
     1081 ggctcgctgg gggacgtgag caagaacatg gccccgctgc caagcgcgcc gcgcaagcgc
     1141 cgggtgctct tctcgcaggc gcaggtgtac gagctggagc gacgcttcaa gcaacagaag
     1201 tacctgtcgg cgccggagcg cgagcacctg gccagcatga tccacctgac gcccacgcag
     1261 gtcaagatct ggttccagaa ccaccgctac aaaatgaagc gccaggccaa ggacaaggcg
     1321 gcgcagcagc aactgcagca ggacagcggc ggcggcgggg gcggcggggg caccgggtgc
     1381 ccgcagcagc aacaggctca gcagcagtcg ccgcgacgcg tggcggtgcc ggtcctggtg
     1441 aaagacggca aaccgtgcca ggcgggtgcc cccgcgccgg gcgccgccag cctacaaggc
     1501 cacgcgcagc agcaggcgca gcaccaggcg caggccgcgc aggcggcggc agcggccatc
     1561 tccgtgggca gcggtggcgc cggccttggc gcacacccgg gccaccagcc aggcagcgca
     1621 ggccagtctc cggacctggc gcaccacgcc gccagccccg cggcgctgca gggccaggta
     1681 tccagcctgt cccacctgaa ctcctcgggc tcggactacg gcaccatgtc ctgctccacc
     1741 ttgctatacg gtcggacctg gtgaggccgc ctaatctttt tccctctgcc aaaaattatg
     1801 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     1861 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     1921 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
     1981 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
     2041 ctgtccattc cttacatatg actagtaagg atctgcgatc gctccggtgc ccgtcagtgg
     2101 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     2161 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     2221 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     2281 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     2341 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     2401 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     2461 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     2521 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     2581 cgttacagat ccaagctgtg accggcgcct acacctgcag ccaacgccac catggtgtct
     2641 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     2701 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     2761 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     2821 accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgacttc
     2881 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     2941 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     3001 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
     3061 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac
     3121 atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga
     3181 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg
     3241 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
     3301 agatactgcg acctccctag caaactgggg cacaaactta atgctagcga gggcagagga
     3361 agtctgctaa catgcggtga cgtcgaggag aatcctggcc caatgtctag actggacaag
     3421 agcaaagtca taaactctgc tctggaatta ctcaatggag tcggtatcga aggcctgacg
     3481 acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag
     3541 aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac
     3601 tcctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac
     3661 cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca
     3721 gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc
     3781 ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg
     3841 gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg
     3901 cccccacttc tgaaacaagc aattgagctg ttcgaccggc agggagccga acctgccttc
     3961 cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc
     4021 gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac
     4081 gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc
     4141 cccgggtaag tcgacgatcc taatcaacct ctggattaca aaatttgtga aagattgact
     4201 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4261 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4321 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4381 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4441 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4501 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     4561 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4621 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4681 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4741 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4801 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     4861 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4921 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4981 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5041 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     5101 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
     5161 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5221 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5281 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5341 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5401 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5461 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5521 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5581 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     5641 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5701 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5761 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5821 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5881 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5941 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6001 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6061 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6121 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6181 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6241 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6301 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6361 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6421 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6481 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6541 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6601 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6661 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6721 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6781 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6841 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6901 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6961 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7021 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7081 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7141 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7201 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag
     7261 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7321 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc
     7381 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7441 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7501 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt
     7561 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     7621 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     7681 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     7741 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
     7801 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     7861 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     7921 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     7981 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     8041 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     8101 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     8161 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     8221 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8281 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8341 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8401 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     8461 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     8521 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     8581 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     8641 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     8701 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     8761 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     8821 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     8881 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
     8941 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     9001 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     9061 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     9121 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     9181 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     9241 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     9301 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     9361 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     9421 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     9481 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     9541 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     9601 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     9661 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     9721 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     9781 ggatctcgac g
//
