LOCUS       sequence-405736-       10169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for human NOTUM 
            overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Lim K, Donovan APA, Tang W, Sun D, He P, Pett JP, Teichmann SA, 
            Marioni JC, Meyer KB, Brand AH, Rawlins EL
  TITLE     Organoid modeling of human fetal lung alveolar development reveals 
            mechanisms of cell fate patterning and neonatal respiratory disease.
  JOURNAL   Cell Stem Cell. 2023 Jan 5;30(1):20-37.e9. doi: 
            10.1016/j.stem.2022.11.013. Epub 2022 Dec 8.
  PUBMED    36493780
REFERENCE   2  (bases 1 to 10169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2022.11.013"; journalName: "Cell Stem Cell"; date: 
            "2023-01-5- 5"; volume: "30"; issue: "1"; pages: "20-37.e9"
COMMENT     Sequence Label: pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G
FEATURES             Location/Qualifiers
     source          1..10169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     212..229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    469..490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(505..510,511..528,529..535)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    543..559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     548..570
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     567..583
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     567..583
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     633..652
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             844..1477
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1524..1649
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2146..2379
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2564..2608
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2901..3018
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3089..3107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3125..3143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3161..3179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3197..3215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3233..3251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3269..3287
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3305..3323
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3368..3392
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(5015..5034)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5080..5135
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5134..5153)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        5339..5550
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5563..5831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5866..5875
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5872..6582
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             6589..6642
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6643..7389
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7402..7990
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7455..7475)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7873..7884)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8065..8298
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8417..8435)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8503..8607
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8608..8676,8677..9468)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8826..8845)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9639..10169,1..58)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10128..10147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
       61 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      121 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      181 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      241 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct
      301 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
      361 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg
      421 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      481 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      541 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac
      601 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
      661 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
      721 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
      781 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
      841 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
      901 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
      961 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     1021 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     1081 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     1141 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     1201 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     1261 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     1321 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     1381 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     1441 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     1501 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     1561 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     1621 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1681 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1741 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1801 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1861 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1921 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1981 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     2041 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     2101 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     2161 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     2221 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     2281 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     2341 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     2401 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     2461 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     2521 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     2581 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     2641 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     2701 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     2761 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2821 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc gaattcacaa
     2881 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2941 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     3001 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggactaga
     3061 ccggtggcct ccacggccac tagattactc cctatcagtg atagagaacg tatgaagagt
     3121 ttactcccta tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa
     3181 cgtataagga gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc
     3241 agtgatagag aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt
     3301 ttactcccta tcagtgatag agaacgtata agctttaggc gtgtacggtg ggcgcctata
     3361 aaagcagagc tcgtttagtg aaccgtcaga tcgcctggag caattccaca acacttttgt
     3421 cttataccaa ctttccgtac cacttcctac cctcgtaaag aagttcctat tccgaagttc
     3481 ctattcttca aaaggtatag gaacttcgct cgaatgggcc gaggggtgcg cgtgctgctg
     3541 ctgctgagcc tgctgcactg cgccgggggc agcgagggca ggaagacctg gcggcgccgg
     3601 ggtcagcagc cgcctcctcc cccgcggacc gaggcggcgc cggcggccgg acagcccgtg
     3661 gagagcttcc cgctggactt cacggccgtg gagggtaaca tggacagctt catggcgcaa
     3721 gtcaagagcc tggcgcagtc cctgtacccc tgctccgcgc agcagctcaa cgaggacctg
     3781 cgcctgcacc tcctactcaa cacctcggtg acctgcaacg acggcagccc cgccggctac
     3841 tacctgaagg agtccagggg cagccggcgg tggctcctct tcctggaagg cggctggtac
     3901 tgcttcaacc gcgagaactg cgactccaga tacgacacca tgcggcgcct catgagctcc
     3961 cgggactggc cgcgcactcg cacaggcaca gggatcctgt cctcacagcc ggaggagaac
     4021 ccctactggt ggaacgcaaa catggtcttc atcccctact gctccagtga tgtttggagc
     4081 ggggcttcat ccaagtctga gaagaacgag tacgccttca tgggcgccct catcatccag
     4141 gaggtggtgc gggagcttct gggcagaggg ctgagcgggg ccaaggtgct gctgctggcc
     4201 gggagcagcg cggggggcac cggggtgctc ctgaatgtgg accgtgtggc tgagcagctg
     4261 gagaagctgg gctacccagc catccaggtg cgaggcctgg ctgactccgg ctggttcctg
     4321 gacaacaagc agtatcgcca cacagactgc gtcgacacga tcacgtgcgc gcccacggag
     4381 gccatccgcc gtggcatcag gtactggaac ggggtggtcc cggagcgctg ccgacgccag
     4441 ttccaggagg gcgaggagtg gaactgcttc tttggctaca aggtctaccc gaccctgcgc
     4501 tgccctgtgt tcgtggtgca gtggctgttt gacgaggcac agctgacggt ggacaacgtg
     4561 cacctgacgg ggcagccggt gcaggagggc ctgcggctgt acatccagaa cctcggccgc
     4621 gagctgcgcc acacactcaa ggacgtgccg gccagctttg cccccgcctg cctctcccat
     4681 gagatcatca tccggagcca ctggacggat gtccaggtga aggggacgtc gctgccccga
     4741 gcactgcact gctgggacag gagcctccat gacagccaca aggccagcaa gacccccctc
     4801 aagggctgcc ccgtccacct ggtggacagc tgcccctggc cccactgcaa cccctcatgc
     4861 cccaccgtcc gagaccagtt cacggggcaa gagatgaacg tggcccagtt cctcatgcac
     4921 atgggcttcg acatgcagac ggtggcccag ccgcagggac tggagcccag tgagctgctg
     4981 gggatgctga gcaacggaag ctagggccgc ctaatctttt tccctctgcc aaaaattatg
     5041 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     5101 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     5161 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
     5221 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
     5281 ctgtccattc cttacatatg actagtaagg atctgcgatc gctccggtgc ccgtcagtgg
     5341 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     5401 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     5461 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     5521 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     5581 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     5641 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     5701 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     5761 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     5821 cgttacagat ccaagctgtg accggcgcct acacctgcag ccaacgccac catggtgtct
     5881 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     5941 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     6001 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     6061 accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgacttc
     6121 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     6181 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     6241 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
     6301 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac
     6361 atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga
     6421 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg
     6481 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
     6541 agatactgcg acctccctag caaactgggg cacaaactta atgctagcga gggcagagga
     6601 agtctgctaa catgcggtga cgtcgaggag aatcctggcc caatgtctag actggacaag
     6661 agcaaagtca taaactctgc tctggaatta ctcaatggag tcggtatcga aggcctgacg
     6721 acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag
     6781 aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac
     6841 tcctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac
     6901 cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca
     6961 gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc
     7021 ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg
     7081 gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg
     7141 cccccacttc tgaaacaagc aattgagctg ttcgaccggc agggagccga acctgccttc
     7201 cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc
     7261 gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac
     7321 gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc
     7381 cccgggtaag tcgacgatcc taatcaacct ctggattaca aaatttgtga aagattgact
     7441 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7501 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7561 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7621 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7681 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7741 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7801 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7861 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7921 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7981 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8041 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     8101 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8161 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8221 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8281 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     8341 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
     8401 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     8461 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8521 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8581 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8641 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8701 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8761 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8821 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     8881 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8941 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     9001 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     9061 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     9121 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9181 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     9241 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9301 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9361 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9421 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9481 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9541 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9601 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9661 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9721 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9781 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9841 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9901 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9961 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10021 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    10081 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10141 tatctttata gtcctgtcgg gtttcgcca
//
