LOCUS       sequence-405737-        9615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) 
            overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Lim K, Donovan APA, Tang W, Sun D, He P, Pett JP, Teichmann SA, 
            Marioni JC, Meyer KB, Brand AH, Rawlins EL
  TITLE     Organoid modeling of human fetal lung alveolar development reveals 
            mechanisms of cell fate patterning and neonatal respiratory disease.
  JOURNAL   Cell Stem Cell. 2023 Jan 5;30(1):20-37.e9. doi: 
            10.1016/j.stem.2022.11.013. Epub 2022 Dec 8.
  PUBMED    36493780
REFERENCE   2  (bases 1 to 9615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2022.11.013"; journalName: "Cell Stem Cell"; date: 
            "2023-01-5- 5"; volume: "30"; issue: "1"; pages: "20-37.e9"
COMMENT     Sequence Label: pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G
FEATURES             Location/Qualifiers
     source          1..9615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      125..713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     614..633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     867..884
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1124..1145
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1160..1165,1166..1183,1184..1190)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1198..1214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1203..1225
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1222..1238
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1222..1238
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1288..1307
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1499..2132
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2179..2304
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2801..3034
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3219..3263
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3556..3673
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3744..3762
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3780..3798
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3816..3834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3852..3870
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3888..3906
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3924..3942
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3960..3978
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4023..4047
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(5116..5135)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5181..5236
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5235..5254)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        5440..5651
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5664..5932
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5967..5976
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5973..6683
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             6690..6743
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6744..7490
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7503..8091
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7556..7576)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7974..7985)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8166..8399
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8518..8536)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8604..8708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8709..8777,8778..9569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8927..8946)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
       61 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      121 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      181 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      241 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      481 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      721 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      781 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      841 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      901 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg
      961 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     1021 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg
     1081 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     1141 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     1201 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata
     1261 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     1321 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
     1381 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
     1441 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
     1501 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
     1561 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     1621 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     1681 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     1741 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     1801 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     1861 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     1921 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     1981 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     2041 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     2101 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     2161 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     2221 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     2281 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     2341 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     2401 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     2461 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     2521 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     2581 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     2641 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     2701 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     2761 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     2821 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     2881 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     2941 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     3001 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     3061 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     3121 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     3181 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     3241 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     3301 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     3361 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     3421 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     3481 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt cacaaatggc
     3541 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     3601 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     3661 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga ctagaccggt
     3721 ggcctccacg gccactagat tactccctat cagtgataga gaacgtatga agagtttact
     3781 ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat
     3841 aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga
     3901 tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact
     3961 ccctatcagt gatagagaac gtataagctt taggcgtgta cggtgggcgc ctataaaagc
     4021 agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt ccacaacact tttgtcttat
     4081 accaactttc cgtaccactt cctaccctcg taaagaagtt cctattccga agttcctatt
     4141 cttcaaaagg tataggaact tcgctcgaat gtcgatgagt ccaaagcaca cgactccgtt
     4201 ctcagtgtct gacatcttga gtcccctgga ggaaagctac aagaaagtgg gcatggaggg
     4261 cggcggcctc ggggctccgc tggcggcgta caggcagggc caggcggcac cgccaacagc
     4321 ggccatgcag cagcacgccg tggggcacca cggcgccgtc accgccgcct accacatgac
     4381 ggcggcgggg gtgccccagc tctcgcactc cgccgtgggg ggctactgca acggcaacct
     4441 gggcaacatg agcgagctgc cgccgtacca ggacaccatg aggaacagcg cctctggccc
     4501 cggatggtac ggcgccaacc cagacccgcg cttccccgcc atctcccgct tcatgggccc
     4561 ggcgagcggc atgaacatga gcggcatggg cggcctgggc tcgctggggg acgtgagcaa
     4621 gaacatggcc ccgctgccaa gcgcgccgca aggacaaggc ggcgcagcag caactgcagc
     4681 aggacagcgg cggcggcggg ggcggcgggg gcaccgggtg cccgcagcag caacaggctc
     4741 agcagcagtc gccgcgacgc gtggcggtgc cggtcctggt gaaagacggc aaaccgtgcc
     4801 aggcgggtgc ccccgcgccg ggcgccgcca gcctacaagg ccacgcgcag cagcaggcgc
     4861 agcaccaggc gcaggccgcg caggcggcgg cagcggccat ctccgtgggc agcggtggcg
     4921 ccggccttgg cgcacacccg ggccaccagc caggcagcgc aggccagtct ccggacctgg
     4981 cgcaccacgc cgccagcccc gcggcgctgc agggccaggt atccagcctg tcccacctga
     5041 actcctcggg ctcggactac ggcaccatgt cctgctccac cttgctatac ggtcggacct
     5101 ggtgaggccg cctaatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     5161 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     5221 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     5281 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     5341 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttacatat
     5401 gactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     5461 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     5521 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     5581 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     5641 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     5701 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     5761 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     5821 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     5881 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     5941 gaccggcgcc tacacctgca gccaacgcca ccatggtgtc taagggcgaa gagctgatta
     6001 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
     6061 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
     6121 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca
     6181 gcagaacctt catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg
     6241 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
     6301 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
     6361 tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc aacaccgaga
     6421 tgctgtaccc cgctgacggc ggcctggaag gcagaagcga catggccctg aagctcgtgg
     6481 gcgggggcca cctgatctgc aacttcaaga ccacatacag atccaagaaa cccgctaaga
     6541 acctcaagat gcccggcgtc tactatgtgg accacagact ggaaagaatc aaggaggccg
     6601 acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta
     6661 gcaaactggg gcacaaactt aatgctagcg agggcagagg aagtctgcta acatgcggtg
     6721 acgtcgagga gaatcctggc ccaatgtcta gactggacaa gagcaaagtc ataaactctg
     6781 ctctggaatt actcaatgga gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     6841 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     6901 atgccctgcc aatcgagatg ctggacaggc atcataccca ctcctgcccc ctggaaggcg
     6961 agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac
     7021 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     7081 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     7141 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
     7201 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgaaacaag
     7261 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     7321 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg
     7381 acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc
     7441 tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa gtcgacgatc
     7501 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7561 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7621 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7681 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7741 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7801 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7861 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     7921 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7981 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     8041 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     8101 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8161 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8221 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8281 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8341 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8401 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8461 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     8521 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8581 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8641 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8701 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8761 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8821 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8881 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8941 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9001 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9061 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9121 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9181 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9241 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9301 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9361 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9421 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9481 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9541 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9601 agattgattt aaaac
//
