LOCUS       sequence-405742-        7348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing mutant TXNIP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Xue Y, Zhou Y, Cepko CL
  TITLE     Txnip deletions and missense alleles prolong the survival of cones 
            in a retinitis pigmentosa mouse model.
  JOURNAL   Elife. 2024 May 10;12:RP90749. doi: 10.7554/eLife.90749.
  PUBMED    38727583
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-05-10"; volume: "12:RP90749. doi"; pages: " 
            10.7554/eLife.90749"
COMMENT     Sequence Label: AAV-CMV-Txnip(C247S)
FEATURES             Location/Qualifiers
     source          1..7348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..134
                     /label=AAV2 ITR
     primer_bind     complement(190..209)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        311..690
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        691..894
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     845..865
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     891..915
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1008..1483
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     complement(2773..2789)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2774..2790)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    2883..3277
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     repeat_region   3632..3761
                     /label=AAV2 ITR
     rep_origin      4132..4645
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4219..4238)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4429..4450
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5127..5146)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5246..5268
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5306..5324)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5392..5496
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5497..5565,5566..6357)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5715..5734)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6528..7116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7017..7036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcagctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac
       61 ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat
      121 cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg
      181 ctctagcggc ctcggcctct gcataaataa aaaaaattag tcagccatga gcttggccca
      241 ttgcatacgt tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta
      301 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      361 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      421 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      481 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      541 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      601 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      661 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      721 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      781 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      841 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      901 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      961 cgggaccgat ccagcctccc ctcgaagctg atcctgagaa cttcagggtg agtctatggg
     1021 acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga
     1081 gaagtaacag ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa
     1141 aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc
     1201 tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa
     1261 taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc
     1321 atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta
     1381 ccattctgct tttattttat ggttgggata aggctggatt attctgagtc caagctaggc
     1441 ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct
     1501 ggtctgtgtg ctggcccatc actttggcaa agaattccgc gggcccggga tccaatatgg
     1561 tgatgttcaa gaagatcaag tcttttgagg tggtcttcaa cgaccccgag aaagtgtacg
     1621 gcagcgggga gaaggtggcc ggacgggtaa ttgtggaagt gtgtgaagtt acccgagtca
     1681 aagctgtcag gatcctggct tgcggcgtgg ccaaggtcct gtggatgcaa gggtctcagc
     1741 agtgcaaaca gactttggac tacttgcgct atgaagacac acttctccta gaagagcagc
     1801 ctacagcagg tgagaacgag atggtgatca tgaggcctgg aaacaaatat gagtacaagt
     1861 tcggcttcga gcttcctcaa gggcccctgg gaacatcctt taaaggaaaa tatggttgcg
     1921 tagactactg ggtgaaggct tttctcgatc gccccagcca gccaactcaa gaggcaaaga
     1981 aaaacttcga agtgatggat ctagtggatg tcaatacccc tgacttaatg gcaccagtgt
     2041 ctgccaaaaa ggagaagaaa gtttcctgca tgttcattcc tgatggacgt gtgtcagtct
     2101 ctgctcgaat tgaccgaaaa ggattctgtg aaggtgatga catctccatc catgctgact
     2161 ttgagaacac gtgttcccga atcgtggtcc ccaaagcggc tattgtggcc cgacacactt
     2221 accttgccaa tggccagacc aaagtgttca ctcagaagct gtcctcggtc agaggcaatc
     2281 acattatctc agggactagc gcatcgtggc gtggcaagag cctcagagtg cagaagatca
     2341 gaccatccat cctgggctgc aacatcctca aagtcgaata ctccttgctg atctacgtca
     2401 gtgtccctgg ctccaagaaa gtcatccttg atctgcccct agtgattggc agcaggtctg
     2461 gtctgagcag ccggacatcc agcatggcca gccggacgag ctctgagatg agctggatag
     2521 acctaaacat cccagatacc ccagaagctc ctccttgcta tatggacatc attcctgaag
     2581 atcacagact agagagcccc accacccctc tgctggacga tgtggacgac tctcaagaca
     2641 gccctatctt tatgtacgcc cctgagttcc agttcatgcc cccacccact tacactgagg
     2701 tggatccgtg cgtccttaac aacaacaaca acaacaacaa cgtgcagtag gctctagagg
     2761 atccaagctt atcgataccg tcgacctcga gggcccagat ctaattcacc ccaccagtgc
     2821 aggctgccta tcagaaagtg gtggctggtg tggctaatgc cctggcccac aagtatcact
     2881 aagctcgctt tcttgctgtc caatttctat taaaggttcc tttgttccct aagtccaact
     2941 actaaactgg gggatattat gaagggcctt gagcatctgg attctgccta ataaaaaaca
     3001 tttattttca ttgcaatgat gtatttaaat tatttctgaa tattttacta aaaagggaat
     3061 gtgggaggtc agtgcattta aaacataaag aaatgaagag ctagttcaaa ccttgggaaa
     3121 atacactata tcttaaactc catgaaagaa ggtgaggctg caaacagcta atgcacattg
     3181 gcaacagccc ctgatgccta tgccttattc atccctcaga aaaggattca agtagaggct
     3241 tgatttggag gttaaagttt tgctatgctg tattttacat tacttattgt tttagctgtc
     3301 ctcatgaatg tcttttcact acccatttgc ttatcctgca tctctcagcc ttgactccac
     3361 tcagttctct tgcttagaga taccaccttt cccctgaagt gttccttcca tgttttacgg
     3421 cgagatggtt tctcctcgcc tggccactca gccttagttg tctctgttgt cttatagagg
     3481 tctacttgaa gaaggaaaaa cagggggcat ggtttgactg tcctgtgagc ccttcttccc
     3541 tgcctccccc actcacagtg acccggaatc cctcgacatc tagagcatgg ctacgtagat
     3601 aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact
     3661 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     3721 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctggcgtaa tagcgaagag
     3781 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgattccgt
     3841 tgcaatggct ggcggtaata ttgttctgga tattaccagc aaggccgata gtttgagttc
     3901 ttctactcag gcaagtgatg ttattactaa tcaaagaagt attgcgacaa cggttaattt
     3961 gcgtgatgga cagactcttt tactcggtgg cctcactgat tataaaaaca cttctcagga
     4021 ttctggcgta ccgttcctgt ctaaaatccc tttaatcggc ctcctgttta gctcccgctc
     4081 tgattctaac gaggaaagca cgttatacgt gctcgtcaaa gcaaccatag tacgcgccct
     4141 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4201 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4261 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4321 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4381 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4441 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4501 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4561 ttaacaaaat attaacgctt acaatttaaa tatttgctta tacaatcttc ctgtttttgg
     4621 ggcttttctg attatcaacc ggggtacata tgattgacat gctagtttta cgattaccgt
     4681 tcatcgattc tcttgtttgc tccagactct caggcaatga cctgatagcc tttgtagaga
     4741 cctctcaaaa atagctaccc tctccggcat gaatttatca gctagaacgg ttgaatatca
     4801 tattgatggt gatttgactg tctccggcct ttctcacccg tttgaatctt tacctacaca
     4861 ttactcaggc attgcattta aaatatatga gggttctaaa aatttttatc cttgcgttga
     4921 aataaaggct tctcccgcaa aagtattaca gggtcataat gtttttggta caaccgattt
     4981 agctttatgc tctgaggctt tattgcttaa ttttgctaat tctttgcctt gcctgtatga
     5041 tttattggat gttggaatcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     5101 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     5161 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     5221 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     5281 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5341 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5401 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5461 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5521 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5581 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5641 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5701 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5761 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5821 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5881 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5941 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6001 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6061 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6121 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6181 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6241 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6301 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6361 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6421 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6481 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6541 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6601 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6661 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6721 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6781 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6841 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6901 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6961 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7021 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7081 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7141 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7201 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7261 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7321 ctccccgcgc gttggccgat tcattaat
//
