LOCUS       sequence-405743-        5411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prokaryotic expressing of AtVIP1 fused with a sequence encoding a 
            T4SS translocation tag in A. tumefaciens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Wang L, Zhao F, Liu H, Chen H, Zhang F, Li S, Sun T, Nekrasov V, 
            Huang S, Dong S
  TITLE     A modified Agrobacterium-mediated transformation for two oomycete 
            pathogens.
  JOURNAL   PLoS Pathog. 2023 Apr 21;19(4):e1011346. doi: 
            10.1371/journal.ppat.1011346. eCollection 2023 Apr.
  PUBMED    37083862
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2023-04-21"; volume: "19(4):e1011346. doi"; pages: 
            " 10.1371/journal.ppat.1011346. eCollection 2023 Apr"
COMMENT     Sequence Label: pV1F
FEATURES             Location/Qualifiers
     source          1..5411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1369..2517)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2816..3610)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3819..4350
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      3827..4437
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 atcaccaccc atacccatat tcttcttccc actatcttta ccactcacag aagctaaaat
       61 cgattcgata ttaaacgacg ccgaactcat ctctccatcc atagaattac tcctgctatg
      121 atgatgattc cctttcttct tctctcctga acttgtagct ataaacttct cctcagtaac
      181 acccaaatca tcgaagaaat cggaatcaac cgagaagcta cgaacatggc gaccgaatct
      241 agcttcgggt tttggaggaa gagaattagg aggaacaaca ccgttcgatg aggtttcttc
      301 cgaatcaacg gacatgggag aagcttgcgg ttgttgttgt gattgttgtg gtggtggtgg
      361 agcgttgagg aagtctagag aagagaaatc gatatcggaa ggatcgaata agaggagatc
      421 gtcgattgat tcgccggaga agaaggtttc agagcgagct cgacggtgat gagagatacg
      481 ttgacgtgga gcttcaggca tatgttctat ttcactgaga atcgtttgat ttggtcctct
      541 tcctcctcct tccatgtcga cactagtaga tctaagctta ccttatctcc ttagctcgca
      601 actaacaccg cctctcccgt tggaagaagt gcgttgtttt atgttgacga ttatcgggag
      661 ggtcggttac tcgaaaattt tcaattgctt ctttatgatt tcaattgaag cgaaaacctt
      721 cgctcggcgt cttggaacaa aacatggacc gagaaccgcg catccctgac aaagcaaccg
      781 gattgacaga ccgcagttgg tcaggtcggc ctcagaacga aaatgctcgc gatcaaactt
      841 tttggctgtc cgctggatgc tttttaacat tcgatgcgtc accgtggaat ctcccccggt
      901 cagcaggctc cacggtacgg tttgacacgc ggcggttcaa gcgaaattgg ttcttctagg
      961 ccatagataa tgatctgatc caaattcttg ctgctgccaa tttgactgcg gcgagaaagt
     1021 tcatcgggtt tctgtcatag cgcgtagcta cccctgttaa ctgtctgatt tcgcagaaaa
     1081 acgctcgacg agattacgct gtctgtaaac ccattcgctg aacgggaagc tttccttccg
     1141 attactcttg gcaggaatat tggcccatgc ctgcttgcgc tttgcaaacg ctcttatcgt
     1201 gttggtatca tatgccttgt ccgccagcag gaacgcactc ttagcgattg tttgtaaaaa
     1261 tgtttcgcca tgcggcggtc atgggcttga cacgctgtca gcgcaagacg gatcgccatg
     1321 gagtaaagcg ctggctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca
     1381 atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc
     1441 gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat
     1501 gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa
     1561 catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg
     1621 gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt
     1681 cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac
     1741 gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt
     1801 cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa
     1861 ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag
     1921 ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac
     1981 gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa
     2041 cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat
     2101 atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc
     2161 ctgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt
     2221 cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc
     2281 gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg
     2341 cacgctgtcg cgctcgatct tggccgtagc ttgctgggcc atcgagccga cggactggaa
     2401 ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga
     2461 aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca
     2521 ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga
     2581 cccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta
     2641 gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag
     2701 cgaccccttg cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg
     2761 gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa
     2821 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
     2881 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
     2941 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
     3001 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
     3061 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
     3121 gggtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
     3181 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
     3241 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
     3301 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
     3361 catcatgccg ttcaaggtgc aggacctttg gaacaggcag ctttccttcc agccatagca
     3421 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
     3481 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
     3541 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
     3601 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
     3661 taattataac aagacgaact ccaattcact gttccttgca ttctaaaact ttaaatacca
     3721 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
     3781 gattttgaaa ccacaattat gggtgatgct gcaactcgag cgggccggga gggttcgaga
     3841 agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa
     3901 aacaaggttt ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa
     3961 aaacgggcgg aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt
     4021 caataggtgc gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc
     4081 tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct
     4141 tgtccacatc atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc
     4201 tggccagctc cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc
     4261 gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt
     4321 ttccgcgagg tatccacaac gccggcggcc gcggtgtctc gcacacggct tcgacggcgt
     4381 ttctggcgcg tttgcagggc catagacggc cgccagccca gcggcgaggg caaccagccc
     4441 ggtgagcgga attctcatag accgcgcgtt gatcgaggtc tgtccgccga cattaattcc
     4501 gctcgcgcat cgtcgtgagc cgttttaatg gatcgggcta tgggccgaac ttctgccata
     4561 acctcgagag cggagatttc attgaacata atcggttccg caacgttgaa gaccaagtta
     4621 gggatattgt tgattttatg gtccaagtgg ttccctgaca acctctcttc tggttgaccg
     4681 tcttgatggg tatacgctac gcccaggact gggcgagtag cgacttcact tcgcatttgc
     4741 gacagttgcg cttcacttaa ttgttctcca tctggaagtg ttgctgcgtg ccgcacggca
     4801 tgagacaaca atttcgcgtt tcgcgagagg attttgaggc gtgaagagaa tttttctaaa
     4861 ggttctgttc gaaccgcgtc ccttgtgaaa cgatgggatt tgctaaaatt agctatattc
     4921 tcagccgact cgacaggggt accgcctctc ttggtgaaat ccatgtagct tgggagcgat
     4981 ggctgcccgt ttgtactcat ctgttggttc gttttgttcc caaactgatt cattgccgat
     5041 tgctgagatg agaattgagc acggttgtaa gaatttccat tcccctgagg aatttctcca
     5101 gctaccacct taagtcggtt cagttcatcc cgcagcgctt cattcaaagc atccctaagt
     5161 tcagcttgtt gctctaaagc ttgaagccgc attttgaggt gtttattttc agtgttcagc
     5221 tctgatgttc ctctctgtaa catagtgact tgagcagaca atgtagtagc ttcattctga
     5281 agtgtctgaa ccttcctctc taactcacca gtatacctaa tcttcctctc tttcgacctc
     5341 gccgcagatt gtctattcgc taaaatcctt ttagctcttt taggatcaag caaagcaagc
     5401 tcagcaagtc t
//
