LOCUS       sequence-405745-        6152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing non-targeting control shRNA in 
            photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Xue Y, Zhou Y, Cepko CL
  TITLE     Txnip deletions and missense alleles prolong the survival of cones 
            in a retinitis pigmentosa mouse model.
  JOURNAL   Elife. 2024 May 10;12:RP90749. doi: 10.7554/eLife.90749.
  PUBMED    38727583
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-05-10"; volume: "12:RP90749. doi"; pages: " 
            10.7554/eLife.90749"
COMMENT     Sequence Label: AAV-RedO-shNC
FEATURES             Location/Qualifiers
     source          1..6152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   259..388
                     /label=AAV2 ITR
     primer_bind     complement(2663..2683)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3081..3092)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3159..3176)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3165..3372
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3460..3589
                     /label=AAV2 ITR
     primer_bind     complement(3608..3625)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3608..3624)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3617..3639)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3766..4221
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3853..3872)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4063..4084
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4247..4351
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4352..4420,4421..5212)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4570..4589)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5383..5971
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5872..5891
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagatcgga attcgccctt aagctagcct acagcagcca
      481 gggtgagatt atgaggctga gctgagaata tcaagactgt accgagtagg gggccttggc
      541 aagtgtggag agcccggcag ctggggcaga gggcggagta cggtgtgcgt ttacggacct
      601 cttcaaacga ggtaggaagg tcagaagtca aaaagggaac aaatgatgtt taaccacaca
      661 aaaatgaaaa tccaatggtt ggatatccat tccaaataca caaaggcaac ggataagtga
      721 tccgggccag gcacagaagg ccatgcaccc gtaggattgc actcagagct cccaaatgca
      781 taggaataga agggtgggtg caggaggctg aggggtgggg aaagggcatg ggtgtttcat
      841 gaggacagag cttccgtttc atgcaatgaa aagagtttgg agacggatgg tggtgactgg
      901 actatacact tacacacggt agcgatggta cactttgtat tatgtatatt ttaccacgat
      961 ctttttaaag tgtcaaaggc aaatggccaa atggttcctt gtcctatagc tgtagcagcc
     1021 atcggctgtt agtgacaaag cccctgagtc aagatgacag cagcccccat aactcctaat
     1081 cggctctccc gcgtggagtc atttaggagt agtcgcatta gagacaagtc caacatctaa
     1141 tcttccaccc tggccagggc cccagctggc agcgagggtg ggagactccg ggcagagcag
     1201 agggcgctga cattggggcc cggcctggct tgggtccctc tggcctttcc ccaggggccc
     1261 tctttccttg gggctttctt gggccgccac tgctcccgct cctctccccc catcccaccc
     1321 cctcaccccc tcgttcttca tatccttctc tagtgctccc tccactttca tccacccttc
     1381 tgcaagagtg tgggaccaca aatgagtttt cacctggcct ggggacacac gtgcccccac
     1441 aggtgctgag tgactttcta ggacagtaat ctgctttagg ctaaaatggg acttgatctt
     1501 ctgttagccc taatcatcaa ttagcagagc cggtgaaggt gcagaaccta ccgcctttcc
     1561 aggcctcctc ccacctctgc cacctccact ctccttcctg ggatgtgggg gctggcacac
     1621 gtgtggccca gggcattggt gggattgcac tgagctgggt cattagcgta atcctggaca
     1681 agggcagaca gggcgagcgg agggccagct ccggggctca ggcaaggctg ggggcttccc
     1741 ccagacaccc cactcctcct ctgctggacc cccacttcat agggcacttc gtgttctcaa
     1801 agggcttcca aatagcatgg tggccttgga tgcccaggga agcctcagag ttgcttatct
     1861 ccctctagac agaaggggaa tctcggtcaa gagggagagg tcgccctgtt caaggccacc
     1921 cagccagctc atggcggtaa tgggacaagg ctggccagcc atcccaccct cagaagggac
     1981 ccggtggggc aggtgatctc agaggaggct cacttctggg tctcacattc ttggatccgg
     2041 ttccaggcct cggccctaaa tagtctccct gggctttcaa gagaaccaca tgagaaagga
     2101 ggattcgggc tctgagcagt ttcaccaccc accccccagt ctgcaaatcc tgacccgtgg
     2161 gtccacctgc cccaaaggcg gacgcaggac agtagaaggg aacagagaac acataaacac
     2221 agagagggcc acagcggctc ccacagtcac cgccaccttc ctggcgggga tgggtggggc
     2281 gtctgagttt ggttcccagc aaatccctct gagccgccct tgcgggctcg cctcaggagc
     2341 aggggagcaa gaggtgggag gaggaggtct aagtcccagg cccaattaag agatcaggta
     2401 gtgtagggtt tgggagcttt taaggtgaag aggcccgggc tgatcccaca ggccagtata
     2461 aagcgccgtg accctcaggt gatgcgccag ggccggctgc cgtcggggac agggctttcc
     2521 atagcgatcc ggcttcgcgc cgtagtctta tcaagagtaa gactacggcg cgaagctttt
     2581 ttggaattcg cggccctagc tttggatcca atcaacctct ggattacaaa atttgtgaaa
     2641 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     2701 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     2761 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     2821 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     2881 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     2941 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3001 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3061 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3121 tgccggctct gcggcctctt ccgcgtcttc gagatctgcc tcgactgtgc cttctagttg
     3181 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     3241 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     3301 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     3361 gcatgctggg gactcgagtt aagggcgaat tcccgataag gatcttccta gagcatggct
     3421 acgtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt
     3481 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     3541 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc cttaattaac
     3601 ctaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     3661 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     3721 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     3781 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3841 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3901 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3961 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4021 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4081 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4141 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4201 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4261 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4321 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4381 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4441 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4501 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4561 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4621 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4681 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4741 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4801 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4861 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4921 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4981 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5041 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5101 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5161 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5221 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5281 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5341 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5401 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5461 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5521 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5581 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5641 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5701 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5761 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5821 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5881 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5941 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6001 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6061 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6121 gcgcagcgag tcagtgagcg aggaagcgga ag
//
