LOCUS       sequence-405764-        6987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing deltion and mutant TXNIP in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Xue Y, Zhou Y, Cepko CL
  TITLE     Txnip deletions and missense alleles prolong the survival of cones 
            in a retinitis pigmentosa mouse model.
  JOURNAL   Elife. 2024 May 10;12:RP90749. doi: 10.7554/eLife.90749.
  PUBMED    38727583
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-05-10"; volume: "12:RP90749. doi"; pages: " 
            10.7554/eLife.90749"
COMMENT     Sequence Label: AAV-RedO-nt.Txnip(C247S)(1-301aa)
FEATURES             Location/Qualifiers
     source          1..6987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   259..388
                     /label=AAV2 ITR
     primer_bind     complement(3498..3518)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3916..3927)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3994..4011)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4000..4207
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4295..4424
                     /label=AAV2 ITR
     primer_bind     complement(4443..4460)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4443..4459)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4452..4474)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4601..5056
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4688..4707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4898..4919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5082..5186
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5187..5255,5256..6047)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5405..5424)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6218..6806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6707..6726
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagatcgga attcgccctt aagctagcct acagcagcca
      481 gggtgagatt atgaggctga gctgagaata tcaagactgt accgagtagg gggccttggc
      541 aagtgtggag agcccggcag ctggggcaga gggcggagta cggtgtgcgt ttacggacct
      601 cttcaaacga ggtaggaagg tcagaagtca aaaagggaac aaatgatgtt taaccacaca
      661 aaaatgaaaa tccaatggtt ggatatccat tccaaataca caaaggcaac ggataagtga
      721 tccgggccag gcacagaagg ccatgcaccc gtaggattgc actcagagct cccaaatgca
      781 taggaataga agggtgggtg caggaggctg aggggtgggg aaagggcatg ggtgtttcat
      841 gaggacagag cttccgtttc atgcaatgaa aagagtttgg agacggatgg tggtgactgg
      901 actatacact tacacacggt agcgatggta cactttgtat tatgtatatt ttaccacgat
      961 ctttttaaag tgtcaaaggc aaatggccaa atggttcctt gtcctatagc tgtagcagcc
     1021 atcggctgtt agtgacaaag cccctgagtc aagatgacag cagcccccat aactcctaat
     1081 cggctctccc gcgtggagtc atttaggagt agtcgcatta gagacaagtc caacatctaa
     1141 tcttccaccc tggccagggc cccagctggc agcgagggtg ggagactccg ggcagagcag
     1201 agggcgctga cattggggcc cggcctggct tgggtccctc tggcctttcc ccaggggccc
     1261 tctttccttg gggctttctt gggccgccac tgctcccgct cctctccccc catcccaccc
     1321 cctcaccccc tcgttcttca tatccttctc tagtgctccc tccactttca tccacccttc
     1381 tgcaagagtg tgggaccaca aatgagtttt cacctggcct ggggacacac gtgcccccac
     1441 aggtgctgag tgactttcta ggacagtaat ctgctttagg ctaaaatggg acttgatctt
     1501 ctgttagccc taatcatcaa ttagcagagc cggtgaaggt gcagaaccta ccgcctttcc
     1561 aggcctcctc ccacctctgc cacctccact ctccttcctg ggatgtgggg gctggcacac
     1621 gtgtggccca gggcattggt gggattgcac tgagctgggt cattagcgta atcctggaca
     1681 agggcagaca gggcgagcgg agggccagct ccggggctca ggcaaggctg ggggcttccc
     1741 ccagacaccc cactcctcct ctgctggacc cccacttcat agggcacttc gtgttctcaa
     1801 agggcttcca aatagcatgg tggccttgga tgcccaggga agcctcagag ttgcttatct
     1861 ccctctagac agaaggggaa tctcggtcaa gagggagagg tcgccctgtt caaggccacc
     1921 cagccagctc atggcggtaa tgggacaagg ctggccagcc atcccaccct cagaagggac
     1981 ccggtggggc aggtgatctc agaggaggct cacttctggg tctcacattc ttggatccgg
     2041 ttccaggcct cggccctaaa tagtctccct gggctttcaa gagaaccaca tgagaaagga
     2101 ggattcgggc tctgagcagt ttcaccaccc accccccagt ctgcaaatcc tgacccgtgg
     2161 gtccacctgc cccaaaggcg gacgcaggac agtagaaggg aacagagaac acataaacac
     2221 agagagggcc acagcggctc ccacagtcac cgccaccttc ctggcgggga tgggtggggc
     2281 gtctgagttt ggttcccagc aaatccctct gagccgccct tgcgggctcg cctcaggagc
     2341 aggggagcaa gaggtgggag gaggaggtct aagtcccagg cccaattaag agatcaggta
     2401 gtgtagggtt tgggagcttt taaggtgaag aggcccgggc tgatcccaca ggccagtata
     2461 aagcgccgtg accctcaggt gatgcgccag ggccggctgc cgtcggggac agggctttcc
     2521 atagcggtac catggtgatg ttcaagaaga tcaagtcttt tgaggtggtc ttcaacgacc
     2581 ccgagaaagt gtacggcagc ggggagaagg tggccggacg ggtaattgtg gaagtgtgtg
     2641 aagttacccg agtcaaagct gtcaggatcc tggcttgcgg cgtggccaag gtcctgtgga
     2701 tgcaagggtc tcagcagtgc aaacagactt tggactactt gcgctatgaa gacacacttc
     2761 tcctagaaga gcagcctaca gcaggtgaga acgagatggt gatcatgagg cctggaaaca
     2821 aatatgagta caagttcggc ttcgagcttc ctcaagggcc cctgggaaca tcctttaaag
     2881 gaaaatatgg ttgcgtagac tactgggtga aggcttttct cgatcgcccc agccagccaa
     2941 ctcaagaggc aaagaaaaac ttcgaagtga tggatctagt ggatgtcaat acccctgact
     3001 taatggcacc agtgtctgcc aaaaaggaga agaaagtttc ctgcatgttc attcctgatg
     3061 gacgtgtgtc agtctctgct cgaattgacc gaaaaggatt ctgtgaaggt gatgacatct
     3121 ccatccatgc tgactttgag aacacgtgtt cccgaatcgt ggtccccaaa gcggctattg
     3181 tggcccgaca cacttacctt gccaatggcc agaccaaagt gttcactcag aagctgtcct
     3241 cggtcagagg caatcacatt atctcaggga ctagcgcatc gtggcgtggc aagagcctca
     3301 gagtgcagaa gatcagacca tccatcctgg gctgcaacat cctcaaagtc gaatactcct
     3361 tgctgatcta cgtcagtgtc cctggctcca agaaagtcat ccttgatctg cccctagtga
     3421 ttggcagcag gtcttagtgg atccaatcaa cctctggatt acaaaatttg tgaaagattg
     3481 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3541 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3601 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3661 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3721 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3781 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3841 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3901 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3961 gctctgcggc ctcttccgcg tcttcgagat ctgcctcgac tgtgccttct agttgccagc
     4021 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4081 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4141 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4201 ctggggactc gagttaaggg cgaattcccg ataaggatct tcctagagca tggctacgta
     4261 gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc
     4321 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     4381 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagccttaa ttaacctaat
     4441 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4501 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4561 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     4621 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4681 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4741 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4801 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4861 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4921 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4981 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5041 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5101 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5161 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5221 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5281 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5341 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5401 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5461 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5521 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5581 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5641 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5701 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5761 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5821 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5881 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5941 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6001 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6061 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6121 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6181 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6241 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6301 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6361 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6421 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6481 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6541 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6601 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6661 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6721 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6781 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6841 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6901 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6961 gcgagtcagt gagcgaggaa gcggaag
//
