LOCUS       sequence-405766-        7306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing FLAG-tagged HSP90AB1 in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Xue Y, Zhou Y, Cepko CL
  TITLE     Txnip deletions and missense alleles prolong the survival of cones 
            in a retinitis pigmentosa mouse model.
  JOURNAL   Elife. 2024 May 10;12:RP90749. doi: 10.7554/eLife.90749.
  PUBMED    38727583
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-05-10"; volume: "12:RP90749. doi"; pages: " 
            10.7554/eLife.90749"
COMMENT     Sequence Label: AAV-RO1.7-Hsp90ab1-FLAG
FEATURES             Location/Qualifiers
     source          1..7306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             4055..4120
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(4189..4209)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   4569..4709
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4569..4698
                     /label=AAV2 ITR
     rep_origin      4784..5239
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4871..4890)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5081..5102
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5256..5275)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5375..5397
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5435..5453)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5521..5625
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5626..5694,5695..6486)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5844..5863)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6657..7245
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7146..7165
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcaa atagaagggt gggtgcagga ggctgagggg tggggaaagg
      181 gcatgggtgt ttcatgagga cagagcttcc gtttcatgca atgaaaagag tttggagacg
      241 gatggtggtg actggactat acacttacac acggtagcga tggtacactt tgtattatgt
      301 atattttacc acgatctttt taaagtgtca aaggcaaatg gccaaatggt tccttgtcct
      361 atagctgtag cagccatcgg ctgttagtga caaagcccct gagtcaagat gacagcagcc
      421 cccataactc ctaatcggct ctcccgcgtg gagtcattta ggagtagtcg cattagagac
      481 aagtccaaca tctaatcttc caccctggcc agggccccag ctggcagcga gggtgggaga
      541 ctccgggcag agcagagggc gctgacattg gggcccggcc tggcttgggt ccctctggcc
      601 tttccccagg ggccctcttt ccttggggct ttcttgggcc gccactgctc ccgctcctct
      661 ccccccatcc caccccctca ccccctcgtt cttcatatcc ttctctagtg ctccctccac
      721 tttcatccac ccttctgcaa gagtgtggga ccacaaatga gttttcacct ggcctgggga
      781 cacacgtgcc cccacaggtg ctgagtgact ttctaggaca gtaatctgct ttaggctaaa
      841 atgggacttg atcttctgtt agccctaatc atcaattagc agagccggtg aaggtgcaga
      901 acctaccgcc tttccaggcc tcctcccacc tctgccacct ccactctcct tcctgggatg
      961 tgggggctgg cacacgtgtg gcccagggca ttggtgggat tgcactgagc tgggtcatta
     1021 gcgtaatcct ggacaagggc agacagggcg agcggagggc cagctccggg gctcaggcaa
     1081 ggctgggggc ttcccccaga caccccactc ctcctctgct ggacccccac ttcatagggc
     1141 acttcgtgtt ctcaaagggc ttccaaatag catggtggcc ttggatgccc agggaagcct
     1201 cagagttgct tatctccctc tagacagaag gggaatctcg gtcaagaggg agaggtcgcc
     1261 ctgttcaagg ccacccagcc agctcatggc ggtaatggga caaggctggc cagccatccc
     1321 accctcagaa gggacccggt ggggcaggtg atctcagagg aggctcactt ctgggtctca
     1381 cattcttgga tccggttcca ggcctcggcc ctaaatagtc tccctgggct ttcaagagaa
     1441 ccacatgaga aaggaggatt cgggctctga gcagtttcac cacccacccc ccagtctgca
     1501 aatcctgacc cgtgggtcca cctgccccaa aggcggacgc aggacagtag aagggaacag
     1561 agaacacata aacacagaga gggccacagc ggctcccaca gtcaccgcca ccttcctggc
     1621 ggggatgggt ggggcgtctg agtttggttc ccagcaaatc cctctgagcc gcccttgcgg
     1681 gctcgcctca ggagcagggg agcaagaggt gggaggagga ggtctaagtc ccaggcccaa
     1741 ttaagagatc aggtagtgta gggtttggga gcttttaagg tgaagaggcc cgggctgatc
     1801 ccacaggcca gtataaagcg ccgtgaccct caggtgatgc gccagggccg gctgccgtcg
     1861 gggacagggc tttccatagc atatgcctga ggaagtgcac catggagagg aggaggtgga
     1921 gacctttgcc tttcaggcag aaattgccca gctcatgtcc ctcatcatca acactttcta
     1981 ttcaaacaag gagattttcc tccgcgagtt gatctctaat gcttcagatg ccctggacaa
     2041 gattcgatat gagagcctga cggacccttc taagttggac agtgggaaag agctgaaaat
     2101 tgacatcatc cccaaccctc aggagcgcac gctgactttg gtggacacag gcattggcat
     2161 gaccaaggct gacctcatta ataacctggg aaccattgct aagtctggca cgaaggcgtt
     2221 catggaggct ctccaggctg gtgcagacat ctccatgatc gggcagtttg gtgtcggatt
     2281 ctactcggcc tatctagttg cagagaaagt ggttgtgatc acgaagcaca atgatgatga
     2341 gcagtatgcc tgggagtcgt ctgcgggtgg ctccttcacc gtccgggcag accatggtga
     2401 gcccattggc cggggtacca aagtgatcct tcacctcaaa gaagaccaga cagagtactt
     2461 ggaggagagg agggtcaagg aagtggtgaa gaaacattcg cagttcatag gctatcccat
     2521 caccctctat ttggagaagg aacgggagaa ggagatcagt gatgatgagg cagaggaaga
     2581 gaaaggtgag aaagaggagg aagataagga ggatgaggag aagcctaaga ttgaagatgt
     2641 gggatccgat gaggaagatg acagcggcaa agacaagaaa aagaaaacaa agaagatcaa
     2701 agagaagtac attgaccagg aggagctgaa caagacaaag cctatctgga ccagaaaccc
     2761 ggatgacatc acgcaggagg agtatggcga attctataag agcctcacca atgactggga
     2821 ggaccacttg gcagtcaagc acttctctgt agaaggtcag ttggaattca gggcattgct
     2881 cttcattccc cggcgggctc ccttcgacct ttttgagaac aagaagaaga agaacaacat
     2941 caaattgtat gtccgccgtg tgttcatcat ggacagctgt gacgagctga tacctgagta
     3001 cctcaacttt atccgcggtg tggttgactc cgaggacctg cccctgaaca tctcccggga
     3061 gatgctgcag cagagcaaga tcctgaaggt catccgcaag aacatcgtca agaagtgcct
     3121 ggagctcttc tccgagctgg ctgaggacaa ggagaactac aagaagttct atgaggcctt
     3181 ctccaaaaat ttaaagcttg gaattcatga agattccact aaccgacgcc gcctctctga
     3241 gctccttcgc tatcacacct ctcagtctgg agatgagatg acctccttgt cagagtatgt
     3301 gtctcgcatg aaggagaccc agaagtccat ctactatatc actggtgaga gcaaagagca
     3361 agtggccaac tctgcctttg tggagcgagt gcggaagcgg ggcttcgagg tggtgtatat
     3421 gactgagcct attgacgagt actgcgtgca gcagctcaag gagtttgatg ggaagagcct
     3481 ggtctcagtg actaaggagg gcctggagct accagaggac gaggaagaga agaagaaaat
     3541 ggaggagagc aaggcaaagt ttgagaatct ctgcaagctc atgaaggaga tcttggacaa
     3601 gaaggttgaa aaggtgacaa tctccaatag gcttgtgtct tcaccctgct gcattgtgac
     3661 aagcacctat ggctggacag ccaacatgga acggatcatg aaggcccagg cactgcgaga
     3721 caactctaca atgggctaca tgatggccaa aaaacacctg gagatcaacc ctgaccaccc
     3781 catcgtggag accctgcggc agaaggctga ggcagacaaa aacgacaaag ctgtcaagga
     3841 cctggtggtg ctgctgtttg aaactgctct gctctcctct ggtttctcac ttgaggatcc
     3901 ccaaacccac tccaaccgca tctaccgcat gattaaacta ggcctgggca tcgatgaaga
     3961 tgaggtcact gcagaggagc ccagtgctgc tgttcctgat gagatccccc ctctggaagg
     4021 cgatgaggat gcctcgcgca tggaagaggt ggatgattat aaggatcatg atggtgatta
     4081 taaggatcat gatatcgact acaaagacga tgacgacaag tagcccgctc gagataatca
     4141 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4201 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4261 tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc
     4321 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4381 gtttatttgt gaaatttgtg atgctattgc tttatttgta accatctagc tttatttgtg
     4441 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     4501 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
     4561 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     4621 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     4681 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     4741 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     4801 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4861 ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4921 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4981 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5041 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5101 ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     5161 tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5221 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5281 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5341 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5401 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     5461 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5521 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5581 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5641 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5701 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5761 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5821 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5881 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5941 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6001 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6061 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6121 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6181 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6241 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6301 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6361 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6421 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6481 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6541 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6601 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6661 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6721 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6781 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6841 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6901 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6961 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7021 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7081 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7141 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7201 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7261 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
