LOCUS       sequence-405774-        6156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing HSP90AB1 shRNA in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Xue Y, Zhou Y, Cepko CL
  TITLE     Txnip deletions and missense alleles prolong the survival of cones 
            in a retinitis pigmentosa mouse model.
  JOURNAL   Elife. 2024 May 10;12:RP90749. doi: 10.7554/eLife.90749.
  PUBMED    38727583
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-05-10"; volume: "12:RP90749. doi"; pages: " 
            10.7554/eLife.90749"
COMMENT     Sequence Label: AAV-RedO-shHsp90ab1(#c)
FEATURES             Location/Qualifiers
     source          1..6156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   259..388
                     /label=AAV2 ITR
     primer_bind     complement(2667..2687)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3085..3096)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3163..3180)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3169..3376
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3464..3593
                     /label=AAV2 ITR
     primer_bind     complement(3612..3629)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3612..3628)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3621..3643)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3770..4225
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3857..3876)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4067..4088
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4251..4355
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4356..4424,4425..5216)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4574..4593)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5387..5975
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5876..5895
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagatcgga attcgccctt aagctagcct acagcagcca
      481 gggtgagatt atgaggctga gctgagaata tcaagactgt accgagtagg gggccttggc
      541 aagtgtggag agcccggcag ctggggcaga gggcggagta cggtgtgcgt ttacggacct
      601 cttcaaacga ggtaggaagg tcagaagtca aaaagggaac aaatgatgtt taaccacaca
      661 aaaatgaaaa tccaatggtt ggatatccat tccaaataca caaaggcaac ggataagtga
      721 tccgggccag gcacagaagg ccatgcaccc gtaggattgc actcagagct cccaaatgca
      781 taggaataga agggtgggtg caggaggctg aggggtgggg aaagggcatg ggtgtttcat
      841 gaggacagag cttccgtttc atgcaatgaa aagagtttgg agacggatgg tggtgactgg
      901 actatacact tacacacggt agcgatggta cactttgtat tatgtatatt ttaccacgat
      961 ctttttaaag tgtcaaaggc aaatggccaa atggttcctt gtcctatagc tgtagcagcc
     1021 atcggctgtt agtgacaaag cccctgagtc aagatgacag cagcccccat aactcctaat
     1081 cggctctccc gcgtggagtc atttaggagt agtcgcatta gagacaagtc caacatctaa
     1141 tcttccaccc tggccagggc cccagctggc agcgagggtg ggagactccg ggcagagcag
     1201 agggcgctga cattggggcc cggcctggct tgggtccctc tggcctttcc ccaggggccc
     1261 tctttccttg gggctttctt gggccgccac tgctcccgct cctctccccc catcccaccc
     1321 cctcaccccc tcgttcttca tatccttctc tagtgctccc tccactttca tccacccttc
     1381 tgcaagagtg tgggaccaca aatgagtttt cacctggcct ggggacacac gtgcccccac
     1441 aggtgctgag tgactttcta ggacagtaat ctgctttagg ctaaaatggg acttgatctt
     1501 ctgttagccc taatcatcaa ttagcagagc cggtgaaggt gcagaaccta ccgcctttcc
     1561 aggcctcctc ccacctctgc cacctccact ctccttcctg ggatgtgggg gctggcacac
     1621 gtgtggccca gggcattggt gggattgcac tgagctgggt cattagcgta atcctggaca
     1681 agggcagaca gggcgagcgg agggccagct ccggggctca ggcaaggctg ggggcttccc
     1741 ccagacaccc cactcctcct ctgctggacc cccacttcat agggcacttc gtgttctcaa
     1801 agggcttcca aatagcatgg tggccttgga tgcccaggga agcctcagag ttgcttatct
     1861 ccctctagac agaaggggaa tctcggtcaa gagggagagg tcgccctgtt caaggccacc
     1921 cagccagctc atggcggtaa tgggacaagg ctggccagcc atcccaccct cagaagggac
     1981 ccggtggggc aggtgatctc agaggaggct cacttctggg tctcacattc ttggatccgg
     2041 ttccaggcct cggccctaaa tagtctccct gggctttcaa gagaaccaca tgagaaagga
     2101 ggattcgggc tctgagcagt ttcaccaccc accccccagt ctgcaaatcc tgacccgtgg
     2161 gtccacctgc cccaaaggcg gacgcaggac agtagaaggg aacagagaac acataaacac
     2221 agagagggcc acagcggctc ccacagtcac cgccaccttc ctggcgggga tgggtggggc
     2281 gtctgagttt ggttcccagc aaatccctct gagccgccct tgcgggctcg cctcaggagc
     2341 aggggagcaa gaggtgggag gaggaggtct aagtcccagg cccaattaag agatcaggta
     2401 gtgtagggtt tgggagcttt taaggtgaag aggcccgggc tgatcccaca ggccagtata
     2461 aagcgccgtg accctcaggt gatgcgccag ggccggctgc cgtcggggac agggctttcc
     2521 atagcgatcc gcctgagtac ctcaacttta tctcaagagg ataaagttga ggtactcagg
     2581 ttttttggaa ttcgcggccc tagctttgga tccaatcaac ctctggatta caaaatttgt
     2641 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2701 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2761 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2821 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2881 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2941 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3001 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     3061 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3121 ctgctgccgg ctctgcggcc tcttccgcgt cttcgagatc tgcctcgact gtgccttcta
     3181 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     3241 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     3301 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     3361 gcaggcatgc tggggactcg agttaagggc gaattcccga taaggatctt cctagagcat
     3421 ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg
     3481 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     3541 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagccttaat
     3601 taacctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     3661 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     3721 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     3781 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3841 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3901 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     3961 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4021 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4081 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4141 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4201 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4261 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4321 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4381 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4441 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4501 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4561 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4621 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4681 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4741 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4801 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4861 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4921 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4981 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5041 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5101 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5161 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5221 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5281 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5341 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5401 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5461 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5521 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5581 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5641 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5701 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5761 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5821 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5881 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5941 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6001 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6061 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6121 ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
