LOCUS       sequence-405775-        5970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing mutant TXNIP in retinal pigment epithelium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Xue Y, Wang SK, Rana P, West ER, Hong CM, Feng H, Wu DM, Cepko CL
  TITLE     AAV-Txnip prolongs cone survival and vision in mouse models of 
            retinitis pigmentosa.
  JOURNAL   Elife. 2021 Apr 13;10. pii: 66240. doi: 10.7554/eLife.66240.
  PUBMED    33847261
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-04-13"; volume: "10. pii: 66240. doi"; pages: " 
            10.7554/eLife.66240"
COMMENT     Sequence Label: AAV-Best1-Txnip(C247S_LL351-352AA)
FEATURES             Location/Qualifiers
     source          1..5970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   259..388
                     /label=AAV2 ITR
     intron          1084..1216
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1199..1218
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(2481..2501)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2899..2910)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2977..2994)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2983..3190
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3278..3407
                     /label=AAV2 ITR
     primer_bind     complement(3426..3443)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3426..3442)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3435..3457)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3584..4039
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3671..3690)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3881..3902
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4065..4169
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4170..4238,4239..5030)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4388..4407)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5201..5789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5690..5709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagatcgga attcgccctt aagctagctc cttttcagat
      481 aagggcactg aggctgagag aggagctgaa acctacccgg ggtcaccaca cacaggtggc
      541 aaggctggga ccagaaacca ggactgttga ctgcagcccg gtattcattc tttccatagc
      601 ccacagggct gtcaaagacc ccagggccta gtcagaggct cctccttcct ggagagttcc
      661 tggcacagaa gttgaagctc agcacagccc cctaaccccc aactctctct gcaaggcctc
      721 aggggtcaga acactggtgg agcagatcct ttagcctctg gattttaggg ccatggtaga
      781 gggggtgttg ccctaaattc cagccctggt ctcagcccaa caccctccaa gaagaaatta
      841 gaggggccat ggccaggctg tgctagccgt tgcttctgag cagattacaa gaagggacta
      901 agacaaggac tcctttgtgg aggtcctggc ttagggagtc aagtgacggc ggctcagcac
      961 tcacgtgggc agtgccagcc tctaagagtg ggcaggggca ctggccacag agtcccaggg
     1021 agtcccacca gcctagtcgc cagacccaga tcctgcagaa gttggtcgtg aggcactggg
     1081 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
     1141 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
     1201 gcctttctct ccacaggtgt ccaggcatgg tgatgttcaa gaagatcaag tcttttgagg
     1261 tggtcttcaa cgaccccgag aaagtgtacg gcagcgggga gaaggtggcc ggacgggtaa
     1321 ttgtggaagt gtgtgaagtt acccgagtca aagctgtcag gatcctggct tgcggcgtgg
     1381 ccaaggtcct gtggatgcaa gggtctcagc agtgcaaaca gactttggac tacttgcgct
     1441 atgaagacac acttctccta gaagagcagc ctacagcagg tgagaacgag atggtgatca
     1501 tgaggcctgg aaacaaatat gagtacaagt tcggcttcga gcttcctcaa gggcccctgg
     1561 gaacatcctt taaaggaaaa tatggttgcg tagactactg ggtgaaggct tttctcgatc
     1621 gccccagcca gccaactcaa gaggcaaaga aaaacttcga agtgatggat ctagtggatg
     1681 tcaatacccc tgacttaatg gcaccagtgt ctgccaaaaa ggagaagaaa gtttcctgca
     1741 tgttcattcc tgatggacgt gtgtcagtct ctgctcgaat tgaccgaaaa ggattctgtg
     1801 aaggtgatga catctccatc catgctgact ttgagaacac gtgttcccga atcgtggtcc
     1861 ccaaagcggc tattgtggcc cgacacactt accttgccaa tggccagacc aaagtgttca
     1921 ctcagaagct gtcctcggtc agaggcaatc acattatctc agggactagc gcatcgtggc
     1981 gtggcaagag cctcagagtg cagaagatca gaccatccat cctgggctgc aacatcctca
     2041 aagtcgaata ctccttgctg atctacgtca gtgtccctgg ctccaagaaa gtcatccttg
     2101 atctgcccct agtgattggc agcaggtctg gtctgagcag ccggacatcc agcatggcca
     2161 gccggacgag ctctgagatg agctggatag acctaaacat cccagatacc ccagaagctc
     2221 ctccttgcta tatggacatc attcctgaag atcacagact agagagcccc accacccctg
     2281 cagcagacga tgtggacgac tctcaagaca gccctatctt tatgtacgcc cctgagttcc
     2341 agttcatgcc cccacccact tacactgagg tggatccgtg cgtccttaac aacaacaaca
     2401 acaacaacaa cgtgcagtag tggatccaat caacctctgg attacaaaat ttgtgaaaga
     2461 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2521 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2581 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2641 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2701 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2761 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2821 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     2881 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     2941 ccggctctgc ggcctcttcc gcgtcttcga gatctgcctc gactgtgcct tctagttgcc
     3001 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     3061 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     3121 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     3181 atgctgggga ctcgagttaa gggcgaattc ccgataagga tcttcctaga gcatggctac
     3241 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg
     3301 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     3361 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagcct taattaacct
     3421 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     3481 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     3541 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     3601 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3661 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3721 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3781 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3841 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     3901 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     3961 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4021 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4081 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4141 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4201 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4261 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4321 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4381 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4441 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4501 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4561 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4621 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4681 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4741 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4801 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4861 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4921 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4981 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5041 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5101 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5161 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5221 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5281 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5341 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5401 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5461 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5521 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5581 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5641 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5701 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5761 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5821 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5881 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     5941 gcagcgagtc agtgagcgag gaagcggaag
//
