LOCUS       sequence-405776-        9403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of secreted protein fused to 
            COMP5AP-AviTag-9xHis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SEMA3E-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3242..3277
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             3287..3310
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4970..5014
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             5015..5041
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(5181..5198)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5187..5298
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5416..5436)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5421..5750
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5601..5736
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5663..5682
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5798..5845
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5864..6463
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5864..5883)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6360..6380
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6593..6714
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6630..6649)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6684..6703
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(6767..7558,7559..7627))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7390..7409
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7628..7719)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7761..7780
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7786..7805
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7891..8479
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8380..8399
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8633..8650
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8748..8765
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8749..8765
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9221..9237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccgctgatac aacacatcct agactgcggc tgagccacaa
     1021 agagctgctg aacctgaacc ggaccagcat ctttcacagc cccttcggct ttctggacct
     1081 gcacacaatg ctgctggacg agtaccaaga gagactgttc gtcggcggca gagatctggt
     1141 gtacagcctg agcctggaac ggatcagcga cggctacaaa gagatccact ggcctagcac
     1201 agccctgaag atggaagagt gcatcatgaa gggcaaagac gccggcgagt gtgccaatta
     1261 tgtgcgggtg ctgcaccact acaacagaac ccatctgctg acctgcggca ccggcgcttt
     1321 tgatcctgtg tgcgccttta tcagagtggg ctaccacctg gaagatcctc tgtttcacct
     1381 ggaaagcccc agaagcgagc gcggcagagg cagatgtcca tttgatccca gcagcagctt
     1441 catcagcacc ctgatcggat ctgagctgtt cgccggcctg tacagcgact attggagcag
     1501 agatgccgcc atcttccggt ctatgggcag actggcccac atcagaaccg agcacgatga
     1561 cgagagactg ctgaaagaac ccaagttcgt gggcagctac atgatccccg acaacgagga
     1621 cagggacgac aacaaggtgt acttcttctt caccgagaag gccctggaag ccgagaacaa
     1681 tgcccacgcc atctacacca gagtggggag actgtgtgtg aacgacgttg gcggccagag
     1741 aatcctggtc aacaagtggt ccaccttcct gaaggccaga ctcgtgtgta gcgtgcccgg
     1801 catgaatggc atcgacacct acttcgacga gctggaagat gtgttcctgc tgcctaccag
     1861 ggaccacaag aaccctgtga tcttcggcct gttcaacacc accagcaaca tcttcagagg
     1921 ccacgccatt tgcgtgtacc acatgtctag catcagagcc gccttcaacg gcccctatgc
     1981 tcacaaagag ggccccgagt atcattggag cgtgtacgag ggcaaagtgc cctatcctag
     2041 acctggcagc tgcgcctcta aagtgaatgg cggcagatac ggcaccacca aggactaccc
     2101 tgacgacgcc atcagattcg ccagatctca ccctctgatg taccaggcca tcaagcccgc
     2161 tcacaagaag cccatcctgg ttaagaccga cggcaagtac aacctgaagc agatcgccgt
     2221 ggacagagtg gaagctgagg atggccagta cgacgtgctg ttcatcggca ccgacaacgg
     2281 catcgtgctg aaagtgatca ccatctacaa tcaagagatg gaatccatgg aagaagtgat
     2341 cctggaagaa ctccagatct tcaaggaccc cgtgcctatc atcagcatgg aaatcagcag
     2401 caagcggcag cagctgtaca tcggctctgc atctgctgtg gcccaagtgc ggttccacca
     2461 ctgtgatatg tatggcagcg cctgcgccga ttgctgcctg gccagagatc catattgtgc
     2521 ctgggatggc atcagctgca gccggtacta tcctacaggc acccacgcca agagaagatt
     2581 cagacggcag gatgtgcggc acggaaatgc cgctcagcag tgctttggac agcagtttgt
     2641 gggcgacgcc ctggacaaga cagaggaaca cctggcctac ggcatcgaga acaacagcac
     2701 actgctggaa tgcacccctc ggagtctgca ggccaaagtg atttggttcg tgcagaaggg
     2761 ccgcgagaca cggaaagagg aagtcaagac agacgaccgc gtggtcaaga tggatctggg
     2821 cctgctgttc ctgagactgc acaagtctga cgccggcacc tacttttgcc agaccgtgga
     2881 acacagcttc gtgcacacag tgcggaagat caccctcgag gtggtggaag aagagaaggt
     2941 cgaggacatg ttcaacaagg acgacgaaga ggaccggcac cacagaatgc cttgtcctgc
     3001 tcagagcagc atctctcagg gcgccaagcc ttggtacaaa gagttcctgc agctcatcgg
     3061 ctacagcaac ttccagcgag tggaagagta ctgcgagaaa gtgtggtgca ccgaccggaa
     3121 gcggaagaaa ctgaagatga gcccctccaa gtggaagtac gccaatcctc aagagaaaaa
     3181 gctgcggagc aagcccgagc actacagact gcctagacac accctggact ctggcgcgcc
     3241 ggaagatcaa gttgatccaa gattgattga tggcaagggc ggatctctgg aggtgctgtt
     3301 ccagggacca ggcggaagcg gcaatcccgg cggcgatctg gccccccaga tgctgcgcga
     3361 gctgcaggag accaatgccg ccctgcagga cgtgcgcgag ctgctgcgcc accgcgtgaa
     3421 ggagatcacc ttcctgaaga acaccgtgat ggagtgcgac gcctgcggta tgcagccctc
     3481 tagaggctcc ggctccatca tcccagttga ggaggagaac ccggacttct ggaaccgcga
     3541 ggcagccgag gccctgggtg ccgccaagaa gctgcagcct gcacagacag ccgccaagaa
     3601 cctcatcatc ttcctgggcg atgggatggg ggtgtctacg gtgacagctg ccaggatcct
     3661 aaaagggcag aagaaggaca aactggggcc tgagataccc ctggccatgg accgcttccc
     3721 atatgtggct ctgtccaaga catacaatgt agacaaacat gtgccagaca gtggagccac
     3781 agccacggcc tacctgtgcg gggtcaaggg caacttccag accattggct tgagtgcagc
     3841 cgcccgcttt aaccagtgca acacgacacg cggcaacgag gtcatctccg tgatgaatcg
     3901 ggccaagaaa gcagggaagt cagtgggagt ggtaaccacc acacgagtgc agcacgcctc
     3961 gccagccggc acctacgccc acacggtgaa ccgcaactgg tactcggacg ccgacgtgcc
     4021 tgcctcggcc cgccaggagg ggtgccagga catcgctacg cagctcatct ccaacatgga
     4081 cattgacgtg atcctaggtg gaggccgaaa gtacatgttt cgcatgggaa ccccagaccc
     4141 tgagtaccca gatgactaca gccaaggtgg gaccaggctg gacgggaaga atctggtgca
     4201 ggaatggctg gcgaagcgcc agggtgcccg gtatgtgtgg aaccgcactg agctcatgca
     4261 ggcttccctg gacccgtctg tgacccatct catgggtctc tttgagcctg gagacatgaa
     4321 atacgagatc caccgagact ccacactgga cccctccctg atggagatga cagaggctgc
     4381 cctgcgcctg ctgagcagga acccccgcgg cttcttcctc ttcgtggagg gtggtcgcat
     4441 cgaccatggt catcatgaaa gcagggctta ccgggcactg actgagacga tcatgttcga
     4501 cgacgccatt gagagggcgg gccagctcac cagcgaggag gacacgctga gcctcgtcac
     4561 tgccgaccac tcccacgtct tctccttcgg aggctacccc ctgcgaggga gctccatctt
     4621 cgggctggcc cctggcaagg cccgggacag gaaggcctac acggtcctcc tatacggaaa
     4681 cggtccaggc tatgtgctca aggacggcgc ccggccggat gttaccgaga gcgagagcgg
     4741 gagccccgag tatcggcagc agtcagcagt gcccctggac gaagagaccc acgcaggcga
     4801 ggacgtggcg gtgttcgcgc gcggcccgca ggcgcacctg gttcacggcg tgcaggagca
     4861 gaccttcata gcgcacgtca tggccttcgc cgcctgcctg gagccctaca ccgcctgcga
     4921 cctggcgccc cccgccggca ccaccgacgc cgcgcacccg ggtggcggag gactgaacga
     4981 catcttcgag gctcagaaaa tcgaatggca cgaacatcac catcaccatc accatcacca
     5041 ttaaggccgg cctgtagtca ctggcacaac gcgttacccg gtaagccaat cgggtataca
     5101 cggtcgtcat actgcagaca gggttcttct actttgcaag atagtcttga gtagtaaaat
     5161 aaatagatag agaaaatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5221 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5281 aaatgaggaa attgcatcac aacactcaac cctatctcgg tctattcttt tgatttataa
     5341 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5401 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5461 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5521 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5581 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5641 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     5701 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     5761 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     5821 gtatatcggc atagtataat acgacaaggt gaggaactaa accatgaccg agtacaagcc
     5881 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     5941 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     6001 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6061 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6121 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6181 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6241 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6301 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacat ccgcgccccg
     6361 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6421 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacacgtgc tacgagattt
     6481 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6541 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     6601 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6661 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6721 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcattac caatgcttaa
     6781 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6841 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagc gctgcgatga
     6901 taccgcgaga accacgctca ccggctccgg atttatcagc aataaaccag ccagccggaa
     6961 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7021 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccatcg
     7081 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7141 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7201 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7261 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7321 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7381 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7441 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7501 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7561 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7621 tactcatatt cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7681 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggtcagt gttacaacca
     7741 attaaccaat tctgaacatt atcgcgagcc catttatacc tgaatatggc tcataacacc
     7801 ccttgctcat gaccaaaatc ccttaacgtg agttacgcgc gcgtcgttcc actgagcgtc
     7861 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7921 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7981 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8041 tctagtgtag ccgtagttag cccaccactt caagaactct gtagcaccgc ctacatacct
     8101 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8161 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8221 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8281 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8341 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8401 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8461 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8521 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8581 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8641 agtgagcgag gaagcggaag gcgagagtag ggaactgcca ggcatcaaac taagcagaag
     8701 gcccctgacg gatggccttt ttgcgtttct acaaactctt tctgtgttgt aaaacgacgg
     8761 ccagtcttaa gctcgggccc cctgggcggt tctgataacg agtaatcgtt aatccgcaaa
     8821 taacgtaaaa acccgcttcg gcgggttttt ttatgggggg agtttaggga aagagcattt
     8881 gtcagaatat ttaagggcgc ctgtcacttt gcttgatata tgagaattat ttaaccttat
     8941 aaatgagaaa aaagcaacgc actttaaata agatacgttg ctttttcgat tgatgaacac
     9001 ctataattaa actattcatc tattatttat gattttttgt atatacaata tttctagttt
     9061 gttaaagaga attaagaaaa taaatctcga aaataataaa gggaaaatca gtttttgata
     9121 tcaaaattat acatgtcaac gataatacaa aatataatac aaactataag atgttatcag
     9181 tatttattat catttagaat aaattttgtg tcgcccttaa ttgtgagcgg ataacaatta
     9241 cgagcttcat gcacagtggc gttgactcaa tattggccat tagccatatt attcattggt
     9301 tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat
     9361 atgtacattt atattggctc atgtccaata tgaccgccat gtt
//
