LOCUS       sequence-405777-        6667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing RPE65 in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Xue Y, Sun X, Wang SK, Collin GB, Kefalov VJ, Cepko CL
  TITLE     Chromophore supply modulates cone function and survival in retinitis
            pigmentosa mouse models.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jun 6;120(23):e2217885120. doi: 
            10.1073/pnas.2217885120. Epub 2023 May 30.
  PUBMED    37252956
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-06-6"; volume: "120(23):e2217885120. 
            doi"; pages: " 10.1073/pnas.2217885120. Epub 2023 May 30"
COMMENT     Sequence Label: AAV-RO1.7-RPE65
FEATURES             Location/Qualifiers
     source          1..6667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     complement(3550..3570)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   3930..4070
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3930..4059
                     /label=AAV2 ITR
     rep_origin      4145..4600
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4232..4251)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4442..4463
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4617..4636)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4736..4758
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4796..4814)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4882..4986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4987..5055,5056..5847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5205..5224)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6018..6606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6507..6526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcaa atagaagggt gggtgcagga ggctgagggg tggggaaagg
      181 gcatgggtgt ttcatgagga cagagcttcc gtttcatgca atgaaaagag tttggagacg
      241 gatggtggtg actggactat acacttacac acggtagcga tggtacactt tgtattatgt
      301 atattttacc acgatctttt taaagtgtca aaggcaaatg gccaaatggt tccttgtcct
      361 atagctgtag cagccatcgg ctgttagtga caaagcccct gagtcaagat gacagcagcc
      421 cccataactc ctaatcggct ctcccgcgtg gagtcattta ggagtagtcg cattagagac
      481 aagtccaaca tctaatcttc caccctggcc agggccccag ctggcagcga gggtgggaga
      541 ctccgggcag agcagagggc gctgacattg gggcccggcc tggcttgggt ccctctggcc
      601 tttccccagg ggccctcttt ccttggggct ttcttgggcc gccactgctc ccgctcctct
      661 ccccccatcc caccccctca ccccctcgtt cttcatatcc ttctctagtg ctccctccac
      721 tttcatccac ccttctgcaa gagtgtggga ccacaaatga gttttcacct ggcctgggga
      781 cacacgtgcc cccacaggtg ctgagtgact ttctaggaca gtaatctgct ttaggctaaa
      841 atgggacttg atcttctgtt agccctaatc atcaattagc agagccggtg aaggtgcaga
      901 acctaccgcc tttccaggcc tcctcccacc tctgccacct ccactctcct tcctgggatg
      961 tgggggctgg cacacgtgtg gcccagggca ttggtgggat tgcactgagc tgggtcatta
     1021 gcgtaatcct ggacaagggc agacagggcg agcggagggc cagctccggg gctcaggcaa
     1081 ggctgggggc ttcccccaga caccccactc ctcctctgct ggacccccac ttcatagggc
     1141 acttcgtgtt ctcaaagggc ttccaaatag catggtggcc ttggatgccc agggaagcct
     1201 cagagttgct tatctccctc tagacagaag gggaatctcg gtcaagaggg agaggtcgcc
     1261 ctgttcaagg ccacccagcc agctcatggc ggtaatggga caaggctggc cagccatccc
     1321 accctcagaa gggacccggt ggggcaggtg atctcagagg aggctcactt ctgggtctca
     1381 cattcttgga tccggttcca ggcctcggcc ctaaatagtc tccctgggct ttcaagagaa
     1441 ccacatgaga aaggaggatt cgggctctga gcagtttcac cacccacccc ccagtctgca
     1501 aatcctgacc cgtgggtcca cctgccccaa aggcggacgc aggacagtag aagggaacag
     1561 agaacacata aacacagaga gggccacagc ggctcccaca gtcaccgcca ccttcctggc
     1621 ggggatgggt ggggcgtctg agtttggttc ccagcaaatc cctctgagcc gcccttgcgg
     1681 gctcgcctca ggagcagggg agcaagaggt gggaggagga ggtctaagtc ccaggcccaa
     1741 ttaagagatc aggtagtgta gggtttggga gcttttaagg tgaagaggcc cgggctgatc
     1801 ccacaggcca gtataaagcg ccgtgaccct caggtgatgc gccagggccg gctgccgtcg
     1861 gggacagggc tttccatagc acatgtctat ccaaattgaa caccctgctg gtggctacaa
     1921 gaaactattt gaaactgtgg aggaactgtc ctcaccacta acagctcatg tcacaggcag
     1981 gattcccctc tggctcactg gcagtctcct ccgatgtggg ccagggctct ttgaagttgg
     2041 atctgagcct ttctatcacc tgtttgatgg acaagccctt ttgcacaagt ttgacttcaa
     2101 ggagggccat gtcacatacc acagaagatt catccgcact gatgcttatg ttcgagcaat
     2161 gactgagaag aggattgtca taacagaatt tggcacctgt gctttcccag acccctgcaa
     2221 gaatatattt tccaggtttt tttcttactt taaaggagta gaggttactg acaatgccct
     2281 tgtaaatatc tacccagtgg gagaagatta ctatgcatgc acagagacca actttatcac
     2341 aaagattaac ccagagacct tggagacaat taagcaggtt gatctttgca actatatttc
     2401 tgtcaatggt gccactgctc atccacatat tgaaagtgat ggaacagttt acaacattgg
     2461 gaattgcttt ggaaaaaatt ttacagttgc ctacaacatt attaagatcc ctccactgaa
     2521 agcagacaag gaagatccaa taaacaagtc agaagttgtt gtgcagttcc cctgcagtga
     2581 tcgtttcaag ccatcttatg tacacagttt tggtctgact cccaactata tcgtttttgt
     2641 ggagactcca gtcaaaatta accttttcaa gtttctttct tcgtggagtc tttggggagc
     2701 caactacatg gactgtttcg agtccaatga aagcatgggg gtttggcttc atgttgctga
     2761 taaaaaaaga agaaaatact tcaataacaa atacaggact tcccctttca atctcttcca
     2821 tcatatcaat acttatgaag acaatggatt tctgattgtg gatctctgtt gctggaaagg
     2881 gtttgaattt gtttataatt acttatattt agccaattta cgtgagaatt gggaagaagt
     2941 taaaagaaat gctatgaagg ctcctcagcc tgaagtcagg agatatgtac ttcctttgac
     3001 aattgacaag gtcgacacag gcagaaattt agtcacactg ccccatacaa ctgccacagc
     3061 cactctgcgc agtgatgaga ccatatggct ggaacctgag gttctctttt cagggcctcg
     3121 tcaagccttt gaatttcctc aaatcaatta ccagaaattt ggagggaaac cttatactta
     3181 tgcatacgga cttgggttga atcactttgt tcctgacaag ctctgtaaga tgaacgtcaa
     3241 aactaaagaa atctggatgt ggcaagagcc agattcttac ccatctgaac ccatctttgt
     3301 ttctcaacca gatgctctgg aagaagatga tggtgtggtt ctgagtgtgg tggtgagccc
     3361 tggggcaggg caaaagcctg catatctcct ggttctgaat gccaaagact tgagtgaaat
     3421 tgccagggct gaagtggaga ctaatatccc tgtgaccttc catggactgt tcaaaagatc
     3481 ctagcccgct cgagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3541 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3601 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct
     3661 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3721 gggcactgac aattccgtgg tgtttatttg tgaaatttgt gatgctattg ctttatttgt
     3781 aaccatctag ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     3841 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3901 agatgtggga ggttttttaa agcggccgca ggaaccccta gtgatggagt tggccactcc
     3961 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4021 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     4081 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     4141 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4201 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
     4261 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4321 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     4381 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4441 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
     4501 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
     4561 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     4621 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4681 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4741 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4801 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4861 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4921 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4981 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5041 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5101 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5161 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5221 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5281 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5341 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5401 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5461 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5521 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5581 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5641 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5701 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5761 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5821 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5881 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5941 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6001 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6061 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6121 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6181 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6241 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6301 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6361 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6421 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6481 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6541 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6601 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6661 cacatgt
//
