LOCUS       sequence-405778-        5761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing LRAT in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Xue Y, Sun X, Wang SK, Collin GB, Kefalov VJ, Cepko CL
  TITLE     Chromophore supply modulates cone function and survival in retinitis
            pigmentosa mouse models.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jun 6;120(23):e2217885120. doi: 
            10.1073/pnas.2217885120. Epub 2023 May 30.
  PUBMED    37252956
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-06-6"; volume: "120(23):e2217885120. 
            doi"; pages: " 10.1073/pnas.2217885120. Epub 2023 May 30"
COMMENT     Sequence Label: AAV-RO1.7-Lrat
FEATURES             Location/Qualifiers
     source          1..5761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     complement(2644..2664)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   3024..3164
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3024..3153
                     /label=AAV2 ITR
     rep_origin      3239..3694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3326..3345)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3536..3557
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3711..3730)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3830..3852
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3890..3908)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3976..4080
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4081..4149,4150..4941)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4299..4318)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5112..5700
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5601..5620
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcaa atagaagggt gggtgcagga ggctgagggg tggggaaagg
      181 gcatgggtgt ttcatgagga cagagcttcc gtttcatgca atgaaaagag tttggagacg
      241 gatggtggtg actggactat acacttacac acggtagcga tggtacactt tgtattatgt
      301 atattttacc acgatctttt taaagtgtca aaggcaaatg gccaaatggt tccttgtcct
      361 atagctgtag cagccatcgg ctgttagtga caaagcccct gagtcaagat gacagcagcc
      421 cccataactc ctaatcggct ctcccgcgtg gagtcattta ggagtagtcg cattagagac
      481 aagtccaaca tctaatcttc caccctggcc agggccccag ctggcagcga gggtgggaga
      541 ctccgggcag agcagagggc gctgacattg gggcccggcc tggcttgggt ccctctggcc
      601 tttccccagg ggccctcttt ccttggggct ttcttgggcc gccactgctc ccgctcctct
      661 ccccccatcc caccccctca ccccctcgtt cttcatatcc ttctctagtg ctccctccac
      721 tttcatccac ccttctgcaa gagtgtggga ccacaaatga gttttcacct ggcctgggga
      781 cacacgtgcc cccacaggtg ctgagtgact ttctaggaca gtaatctgct ttaggctaaa
      841 atgggacttg atcttctgtt agccctaatc atcaattagc agagccggtg aaggtgcaga
      901 acctaccgcc tttccaggcc tcctcccacc tctgccacct ccactctcct tcctgggatg
      961 tgggggctgg cacacgtgtg gcccagggca ttggtgggat tgcactgagc tgggtcatta
     1021 gcgtaatcct ggacaagggc agacagggcg agcggagggc cagctccggg gctcaggcaa
     1081 ggctgggggc ttcccccaga caccccactc ctcctctgct ggacccccac ttcatagggc
     1141 acttcgtgtt ctcaaagggc ttccaaatag catggtggcc ttggatgccc agggaagcct
     1201 cagagttgct tatctccctc tagacagaag gggaatctcg gtcaagaggg agaggtcgcc
     1261 ctgttcaagg ccacccagcc agctcatggc ggtaatggga caaggctggc cagccatccc
     1321 accctcagaa gggacccggt ggggcaggtg atctcagagg aggctcactt ctgggtctca
     1381 cattcttgga tccggttcca ggcctcggcc ctaaatagtc tccctgggct ttcaagagaa
     1441 ccacatgaga aaggaggatt cgggctctga gcagtttcac cacccacccc ccagtctgca
     1501 aatcctgacc cgtgggtcca cctgccccaa aggcggacgc aggacagtag aagggaacag
     1561 agaacacata aacacagaga gggccacagc ggctcccaca gtcaccgcca ccttcctggc
     1621 ggggatgggt ggggcgtctg agtttggttc ccagcaaatc cctctgagcc gcccttgcgg
     1681 gctcgcctca ggagcagggg agcaagaggt gggaggagga ggtctaagtc ccaggcccaa
     1741 ttaagagatc aggtagtgta gggtttggga gcttttaagg tgaagaggcc cgggctgatc
     1801 ccacaggcca gtataaagcg ccgtgaccct caggtgatgc gccagggccg gctgccgtcg
     1861 gggacagggc tttccatagc acatgaagaa cccaatgctg gaagctgcgt ccctcctcct
     1921 ggagaaactg ctccttattt ccaacttcaa gctctttagc gtgagcgtcc cgggaggagg
     1981 cacagggaag aaccgtccct atgaaatcag ctctttcgtc cggggcgacg tgttggaggt
     2041 gtcacggacc cattttatcc actatgggat ctacctgggg gaaaaccgtg tcgcccatct
     2101 aatgcctgac atcctgttgg ccctgaccaa tgacaaggaa cgcactcaga aggtggtctc
     2161 caacaagcgt ctcctcctgg gagtcatttg caaggtggct agcatccgtg tggacacagt
     2221 agaggacttt gcctacggag cagacatcct agtcaatcac ctagacggga ctctcaagaa
     2281 gaagtcctta ctcaatgagg aggtggcacg cagagctgag cagcagttgg gactgactcc
     2341 atacagccta ctgtggaaca actgcgaaca ctttgtgact tactgcagat atggctctcg
     2401 gatcagtcca caggctgaga agttctatga caccgtgaag ataatcattc gtgatcagag
     2461 aagcagtctt gcttcggctg tcttgggatt agcatctatt gtctacacag gcctggcatc
     2521 atatatgacc cttcctgcaa tctgtatccc attctgcttg tggatgatgt ctggctagcc
     2581 cgctcgagat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     2641 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     2701 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac
     2761 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     2821 tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     2881 ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     2941 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
     3001 gggaggtttt ttaaagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     3061 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3121 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     3181 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     3241 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3301 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3361 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3421 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     3481 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3541 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     3601 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3661 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     3721 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     3781 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     3841 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     3901 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     3961 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4021 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4081 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4141 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4201 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4261 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4321 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4381 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4441 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4501 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4561 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4621 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4681 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4741 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4801 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4861 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4921 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4981 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5041 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5101 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5161 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5221 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5281 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5341 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5401 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5461 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5521 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5581 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5641 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5701 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5761 t
//
