LOCUS       sequence-405779-       10378 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10378)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NOTCH4(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..10378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5954..5998
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             5999..6016
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6156..6173)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6162..6273
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6391..6411)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6396..6725
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6576..6711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6638..6657
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6773..6820
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6839..7438
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6839..6858)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7335..7355
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7568..7689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7605..7624)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7659..7678
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(7742..8533,8534..8602))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8365..8384
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8603..8694)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8736..8755
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8761..8780
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8866..9454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9355..9374
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9608..9625
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9724..9740
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10196..10212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccagaggact gctgtgtgga tcttttccag agccttgcgc
     1021 caatggcggc acatgtctgt ctctgtctct cggccaggga acctgtcagt gtgctcctgg
     1081 atttctgggc gagacttgtc agttccccga tccttgtcag aacgcccagc tgtgtcagaa
     1141 tggcggctct tgtcaagctc tgctgcctgc tcctctggga ctgccttctt ctccatctcc
     1201 actgacaccc agctttctgt gcacctgtct gcctggcttt accggcgaaa gatgccaggc
     1261 caagctggaa gatccctgtc ctcctagctt ctgcagcaag aggggcagat gtcacatcca
     1321 ggcaagcggc agacctcagt gttcttgtat gcctggctgg acaggcgagc agtgccagct
     1381 gagagatttc tgcagcgcca atccttgtgt gaacggcggc gtgtgtctgg ccacatatcc
     1441 tcagatccag tgccactgtc ctccaggctt tgagggacac gcctgcgaga gggatgtgaa
     1501 cgagtgtttt caggaccccg gaccttgtcc taagggcacc tcttgtcaca acaccctggg
     1561 cagcttccag tgtctgtgtc ctgtgggaca agagggaccc agatgtgaac tgagagccgg
     1621 gccttgtcca cctagaggct gttctaatgg cgggacctgt cagctgatgc ccgagaagga
     1681 tagcaccttc cacctgtgtc tgtgcccacc tggcttcatc ggccccgatt gcgaagtgaa
     1741 ccccgacaat tgtgtgtccc accagtgcca gaacggcgga acttgtcagg acggcctgga
     1801 cacctatacc tgcctgtgtc cagagacatg gaccggctgg gattgcagcg aggatgtgga
     1861 tgagtgtgaa acacagggcc ctccacactg cagaaacggc gggacatgcc agaatagcgc
     1921 cggctctttt cactgcgtgt gtgtgtcagg ctggggcggc acaagctgcg aggaaaatct
     1981 ggacgattgc attgccgcca catgcgcccc tggcagcaca tgtattgata gagtgggctc
     2041 cttcagctgt ctctgccctc ctggaagaac aggcctgctg tgtcacctgg aagatatgtg
     2101 cctgagccag ccttgtcatg gcgacgccca gtgtagcaca aatcctctga ccggcagcac
     2161 cctgtgtctt tgtcagcctg gctactctgg ccctacctgc caccaggatc tggatgaatg
     2221 tctgatggcc cagcagggcc cttctccttg tgaacatggc ggaagctgtc tgaacacacc
     2281 cggcagcttc aactgcttgt gtcctcctgg ctacaccgga agcagatgcg aggccgatca
     2341 caacgaatgc ctgtctcagc cttgccatcc tggctctacc tgtctggatc tgctggccac
     2401 ctttcactgc ctctgtccgc ctggactgga aggacagctg tgcgaggtgg aaacaaatga
     2461 gtgcgccagc gctccttgcc tgaatcacgc cgattgccac gacctgctga acggctttca
     2521 gtgtatctgc ctgcctggat tcagcggcac cagatgcgaa gaggacatcg acgagtgtag
     2581 aagcagccct tgtgccaacg gtggccagtg tcaagatcag ccaggcgcct tccactgcaa
     2641 gtgtctgcca ggatttgaag gccccagatg ccagaccgaa gtggacgagt gtctgagcga
     2701 tccatgtcca gtgggagcct cttgtcttga cctgcctggc gccttctttt gtctttgccc
     2761 tagcggcttt acagggcagc tgtgtgaagt gcccctgtgc gctcctaatc tgtgccagcc
     2821 taaacagatc tgcaaggacc agaaggataa ggccaactgc ctttgtcctg atggctctcc
     2881 aggatgcgcc ccacctgagg ataattgcac atgtcaccac ggccactgcc agagaagctc
     2941 ttgtgtgtgt gatgtcggat ggacaggacc cgagtgtgaa gccgaactcg gcggatgtat
     3001 tagcgcccca tgtgctcacg gtggcacctg ttatccacag ccaagcggct acaactgcac
     3061 atgccctaca ggctacacag gcccaacctg ctctgaggaa atgacagcct gtcacagcgg
     3121 cccatgcctt aacggcgggt cttgtaatcc ttctcctggc ggctactact gtacctgtcc
     3181 accatctcac accgggcctc agtgtcagac aagcaccgac tactgtgtgt ctgccccttg
     3241 ctttaacggt ggaacctgcg tgaacagacc tggcacattc tcttgcctgt gcgccatggg
     3301 ctttcagggg cctagatgtg aaggcaagct gaggccttcc tgcgccgact ctccctgtag
     3361 aaatagagcc acctgtcagg acagccctca gggcccaaga tgtttgtgtc caaccggcta
     3421 taccggcggc agctgtcaga cactgatgga cctgtgtgcc cagaagcctt gtcctcggaa
     3481 tagccactgt ctgcaaacag gcccctcctt ccattgcctc tgcctgcaag gatggaccgg
     3541 gccactgtgt aatctgcctc tcagctcctg tcagaaggcc gcactgtctc agggcatcga
     3601 tgtgtccagt ctctgccaca atggcggact gtgtgttgat agcggcccca gctacttctg
     3661 tcactgccca ccaggttttc agggctccct gtgtcaggat cacgtgaacc cttgcgagag
     3721 cagaccttgc cagaatgggg ccacttgtat ggctcagcct tctggctatc tgtgtcagtg
     3781 cgccccaggc tacgatggcc agaattgcag caaagagctg gacgcctgtc agagccagcc
     3841 atgccacaat cacggcacat gcacacctaa gcctggcgga tttcactgtg catgccctcc
     3901 aggattcgtg ggacttcgct gtgaaggcga cgttgacgaa tgtctggacc agccatgtca
     3961 ccctactgga acagccgcct gtcattctct ggccaacgcc ttctattgcc agtgtctccc
     4021 tggacacaca ggccagtggt gcgaggtcga gattgatcct tgccactctc agccctgctt
     4081 tcacggcggt acatgtgaag ctacagccgg ctctcccctg ggcttcattt gtcactgtcc
     4141 caagggattc gagggcccca catgttctca cagagcccct agctgtggct tccaccactg
     4201 tcatcacggc ggactctgtc tgccatctcc taagccaggc ttcccaccta gatgcgcctg
     4261 tctctctgga tatggcggcc ctgattgtct gacccctcct gctcctaaag gctgtggccc
     4321 tccttctcca tgcctgtaca atggcagctg tagcgagaca acaggcctcg gcggaccagg
     4381 ctttagatgt agctgtcctc acagcagccc tggaccaagg tgtcagaaac ctggcgctaa
     4441 gggctgcgaa ggcagatctg gcgacggtgc atgtgatgct ggatgttctg gacctggcgg
     4501 aaactgggat ggcggagatt gctctctggg agtccctgat ccttggaagg gatgccctag
     4561 ccacagcaga tgctggctgc tgttcagaga tggccagtgc catcctcagt gcgacagcga
     4621 agagtgcctg ttcgatggct acgactgcga gacaccacca gcctgtacac ccgcctacga
     4681 tcagtactgc cacgatcact tccacaacgg acactgcgag aagggctgca ataccgccga
     4741 atgtggatgg gacggtggcg attgcagacc agaggatggc gatcctgaat ggggaccttc
     4801 tctcgctctg ctggttgtgc ttagccctcc agctctggat cagcagctgt ttgctctggc
     4861 tagagtgctg agcctgactc tgagagttgg cctgtgggtc cgaaaggacc gcgacggcag
     4921 agatatggtg tacccttatc caggcgccag agccgaggaa aagctcggcg gaacaagaga
     4981 tcccacctac caagaaagag ccgctcctca gacacagccc ctgggcaaag agacagattc
     5041 cctgagcgcc ggatttgtgg tggtcatggg agtcgatctg tccagatgtg gacctgatca
     5101 ccccgccagt agatgtcctt gggaccctgg actgctgctg agattccttg ctgctatggc
     5161 tgccgtggga gcacttgaac cattgctgcc tggaccactg ctggctgtgc atcctcatgc
     5221 tggaaccgct cctccagcta accagcttcc ttggggcgcg ccgggcggcg acaagaccca
     5281 cacctgcccc ccctgcccag ctccagaact gctgggaggg ccttccgtgt tcctgttccc
     5341 ccccaagccc aaggacaccc tgatgatcag caggaccccc gaggtgacct gcgtggtggt
     5401 ggccgtgtcc cacgaggacc cagaggtgaa gttcaactgg tacgtggacg gcgtggaggt
     5461 gcacaacgcc aagaccaagc ccagagagga gcagtacaac agcacctaca gggtggtgtc
     5521 cgtgctgacc gtgctgcacc aggactggct gaacggcaaa gaatacaagt gcaaagtctc
     5581 caacaaggcc ctggctgccc caatcgaaaa gacaatcagc aaggccaagg gccagccacg
     5641 ggagccccag gtgtacaccc tgccccccag ccgggaggag atgaccaaga accaggtgtc
     5701 cctgacctgt ctggtgaagg gcttctaccc cagcgatatc gccgtggagt gggagagcaa
     5761 cggccagccc gagaacaact acaagaccac ccccccagtg ctggacagcg acggcagctt
     5821 cttcctgtac agcaagctga ccgtggacaa gtccaggtgg cagcagggca acgtgttcag
     5881 ctgcagcgtg atgcacgagg ccctgcacaa ccactacacc cagaagtccc tgagcctgag
     5941 ccccggcggc agcggtctta atgatatttt cgaggctcag aagattgagt ggcatgaaca
     6001 ccaccaccat caccactaag gccggcctgt agtcactggc acaacgcgtt acccggtaag
     6061 ccaatcgggt atacacggtc gtcatactgc agacagggtt cttctacttt gcaagatagt
     6121 cttgagtagt aaaataaata gatagagaaa atcagcctcg actgtgcctt ctagttgcca
     6181 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6241 tgtcctttcc taataaaatg aggaaattgc atcacaacac tcaaccctat ctcggtctat
     6301 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6361 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     6421 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6481 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6541 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6601 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6661 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     6721 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     6781 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     6841 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     6901 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     6961 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     7021 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     7081 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     7141 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     7201 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     7261 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     7321 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     7381 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaca
     7441 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7501 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7561 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7621 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7681 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7741 attaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7801 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7861 ccagcgctgc gatgataccg cgagaaccac gctcaccggc tccggattta tcagcaataa
     7921 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7981 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8041 acgttgttgc catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8101 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8161 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8221 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8281 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8341 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8401 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8461 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8521 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8581 cacggaaatg ttgaatactc atattcttcc tttttcaata ttattgaagc atttatcagg
     8641 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8701 tcagtgttac aaccaattaa ccaattctga acattatcgc gagcccattt atacctgaat
     8761 atggctcata acaccccttg ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc
     8821 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8881 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8941 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     9001 accaaatact gttcttctag tgtagccgta gttagcccac cacttcaaga actctgtagc
     9061 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9121 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9181 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9241 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9301 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9361 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9421 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9481 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9541 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9601 cgagcgcagc gagtcagtga gcgaggaagc ggaaggcgag agtagggaac tgccaggcat
     9661 caaactaagc agaaggcccc tgacggatgg cctttttgcg tttctacaaa ctctttctgt
     9721 gttgtaaaac gacggccagt cttaagctcg ggccccctgg gcggttctga taacgagtaa
     9781 tcgttaatcc gcaaataacg taaaaacccg cttcggcggg tttttttatg gggggagttt
     9841 agggaaagag catttgtcag aatatttaag ggcgcctgtc actttgcttg atatatgaga
     9901 attatttaac cttataaatg agaaaaaagc aacgcacttt aaataagata cgttgctttt
     9961 tcgattgatg aacacctata attaaactat tcatctatta tttatgattt tttgtatata
    10021 caatatttct agtttgttaa agagaattaa gaaaataaat ctcgaaaata ataaagggaa
    10081 aatcagtttt tgatatcaaa attatacatg tcaacgataa tacaaaatat aatacaaact
    10141 ataagatgtt atcagtattt attatcattt agaataaatt ttgtgtcgcc cttaattgtg
    10201 agcggataac aattacgagc ttcatgcaca gtggcgttga ctcaatattg gccattagcc
    10261 atattattca ttggttatat agcataaatc aatattggct attggccatt gcatacgttg
    10321 tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc gccatgtt
//
