LOCUS       sequence-405780-       10918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NOTCH3(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..10918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      2526..2535
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3781..3790
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6494..6538
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6539..6556
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6696..6713)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6702..6813
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6931..6951)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6936..7265
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7116..7251
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7178..7197
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7313..7360
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7379..7978
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7379..7398)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7875..7895
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8108..8229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8145..8164)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8199..8218
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8282..9073,9074..9142))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8905..8924
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9143..9234)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9276..9295
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9301..9320
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9406..9994
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9895..9914
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10148..10165
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     10263..10280
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10264..10280
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10736..10752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccgctcctcc ttgtcttgat ggatctcctt gtgccaacgg
     1021 cggcagatgt acacagctgc ctagcagaga agccgcctgt ctttgtcctc ctggctgggt
     1081 tggagaacgg tgccagctgg aagatccttg tcactctgga ccttgtgccg gcagaggcgt
     1141 gtgtcaatct tctgtggtgg ccggcacagc ccggttcagc tgtagatgtc ctagaggctt
     1201 cagaggcccc gactgtagcc tgcctgatcc atgtctcagc agcccttgtg ctcatggcgc
     1261 cagatgttct gtgggccctg atggcagatt cctgtgtagc tgtcctccag gctaccaggg
     1321 cagatcctgc agatccgatg tggatgagtg tagagtgggc gagccttgta gacacggcgg
     1381 cacttgtctg aatacccctg gcagcttcag atgccagtgt cctgccggat atacaggccc
     1441 tctgtgcgaa aatcctgccg tgccatgtgc accctctcca tgcagaaatg gcggcacctg
     1501 tagacagagc ggcgacctga catacgattg tgcctgtctg cctggcttcg agggccagaa
     1561 ctgcgaagtg aatgtggacg attgccctgg ccacagatgc ctgaatggcg gaacatgtgt
     1621 ggacggcgtg aacacctaca actgtcagtg tccacctgag tggaccggcc agttctgtac
     1681 tgaggatgtc gacgagtgtc agctgcagcc caatgcctgt cataacggcg gaacctgctt
     1741 taacacactc ggcggccata gctgcgtgtg tgtgaatgga tggacaggcg agagctgctc
     1801 ccagaacatc gatgattgtg ccaccgccgt gtgctttcac ggcgccacat gtcacgatag
     1861 agtggccagc ttttactgcg cctgtcctat gggcaagacc ggcctgctgt gtcacctgga
     1921 tgacgcctgt gtgtccaatc cttgccacga ggacgccatc tgcgacacaa accctgtgaa
     1981 cggcagagcc atctgtacat gcccacctgg ctttaccggc ggagcctgtg atcaggacgt
     2041 ggacgagtgt tctatcggcg ccaatccatg cgagcacctg ggcagatgtg tgaacacaca
     2101 gggcagcttt ctgtgtcagt gcggcagagg atacaccgga cctagatgcg agacagatgt
     2161 gaacgagtgc ctgagcggcc cctgcagaaa tcaggctacc tgtctggacc ggatcggcca
     2221 gtttacctgt atctgcatgg ccggcttcac cggcacctat tgcgaggtgg acattgatga
     2281 gtgccagagc agtccttgcg tgaacggcgg agtgtgcaag gacagagtga atggcttcag
     2341 ctgcacatgc ccaagcggct tttctggcag cacctgtcag ctggacgtcg acgaatgtgc
     2401 cagcacacca tgtagaaacg gcgctaagtg cgtggaccag cctgatggct acgagtgtag
     2461 atgtgccgag ggctttgagg gcaccctgtg cgacagaaat gtcgatgact gcagccccga
     2521 tccatgccac catggtagat gtgtggatgg aatcgccagc ttctcctgtg cttgtgcccc
     2581 tggctataca ggcaccagat gcgaatccca ggtggacgaa tgcagaagcc agccttgcag
     2641 gcatggcggc aaatgcctgg acctggtgga caagtacctg tgtagatgcc cttctggcac
     2701 cacaggcgtg aactgtgaag tcaacatcga cgactgcgcc tctaatccct gcaccttcgg
     2761 cgtgtgcaga gatggcatca acagatacga ctgcgtgtgc cagccaggct tcacaggacc
     2821 cctgtgcaac gtggaaatca acgagtgcgc ttctagccca tgtggcgaag gcggatcttg
     2881 tgtcgatggc gagaacggct ttcggtgtct gtgccctcct ggatctctgc ctcctctgtg
     2941 tctgcctcca tctcacccat gtgctcacga gccatgcagc cacggcatct gttatgatgc
     3001 cccaggcgga ttcagatgcg tgtgcgaacc tggatggtcc gggcctagat gttctcagtc
     3061 tctggctaga gatgcctgcg agtctcagcc atgtagagct ggcggcacat gcagctctga
     3121 tggcatgggc tttcactgca cgtgccctcc aggtgttcag ggcagacaat gcgagctgct
     3181 gagcccctgt acacctaatc catgtgaaca cggcggaaga tgcgagtctg ctcctggaca
     3241 actgccagtc tgttcttgcc ctcaaggatg gcagggacca agatgccagc aggatgttga
     3301 tgaatgcgct ggccctgctc cttgtggccc tcacggaatc tgtacaaatc tggccggctc
     3361 ctttagctgt acctgccacg gtggctatac cggaccaagc tgcgaccagg acatcaacga
     3421 ttgcgaccca aatccatgcc tcaacggcgg gtcttgtcag gatggcgtgg gcagcttctc
     3481 ttgtagctgc ctgcctggat tcgctggccc cagatgtgcc cgagatgtcg atgagtgcct
     3541 gtctaatcct tgcggccctg gcacctgtac agaccacgtg gcaagcttca catgcacctg
     3601 tccgcctgga tacggcggat ttcactgtga acaggacctg ccagattgca gccccagcag
     3661 ctgttttaat ggcgggactt gcgtcgacgg ggtcaacagc ttttcctgtc tgtgcagacc
     3721 aggctacact ggcgcccact gtcagcatga agctgaccct tgtctgagca ggccatgtct
     3781 ccatggcggc gtgtgttctg ccgctcatcc aggctttaga tgcacttgcc tggaatcctt
     3841 cacaggccca cagtgccaga cactggtgga ttggtgtagc agacagccct gccaaaatgg
     3901 tggcagatgc gttcagacag gcgcctactg tttgtgtcct cctggatggt ctggccggct
     3961 gtgcgatatc agaagcctgc cttgtagaga ggccgctgct cagatcggcg ttagactgga
     4021 acaactgtgt caggctggcg gccagtgtgt tgatgaggat agcagccact actgtgtgtg
     4081 ccctgaggga agaaccggct ctcactgcga acaagaggtg gacccatgtc tggcccagcc
     4141 gtgtcagcac ggtggaacat gtagaggcta catgggcggc tacatgtgcg agtgtctgcc
     4201 cggctacaac ggcgacaatt gcgaggatga tgttgacgag tgcgccagcc aaccttgtca
     4261 acatggcggc tcttgcatcg atctggtggc cagatacctg tgcagttgcc caccaggcac
     4321 actcggagtg ctgtgcgaga ttaacgagga cgattgtggc cccggacctc ctctggatag
     4381 tggccctaga tgtctgcaca acgggacctg tgtggatctc gtcggaggct tccgatgcac
     4441 atgtccaccg ggctatactg gactgagatg cgaggccgat atcaatgagt gcagaagtgg
     4501 cgcttgtcac gccgctcaca ccagagattg tctgcaagat cccggcggag gattccgctg
     4561 tctctgtcat gctggattca gcggccctag gtgtcagaca gtgctgtccc cttgtgaaag
     4621 ccagccatgc caacacggtg gccagtgcag accttctcca ggacctggcg gaggcctgac
     4681 ctttacatgt cactgtgccc agcctttctg gggcccaaga tgcgaaagag tggcccggtc
     4741 ctgtagagaa ctccagtgtc cagtgggagt gccttgccag caaacaccta gaggacccag
     4801 atgtgcttgc cctcctggcc tctctggacc tagctgtaga agctttccag gatctccacc
     4861 aggcgccagc aatgcctctt gtgctgctgc tccatgcctt catggtggaa gctgtagacc
     4921 tgctcctctg gcacccttct tcagatgtgc atgcgcccaa ggctggactg ggccaagatg
     4981 tgaagcacca gctgctgccc ctgaggtgtc cgaagaacct cgatgtccaa gagctgcctg
     5041 tcaggccaaa aggggcgacc agagatgcga cagagagtgc aattctcccg gctgtggatg
     5101 ggatggcggc gattgcagtc tgtctgtggg agatccttgg agacagtgcg aagccctgca
     5161 gtgctggcgg ctgttcaaca acagcagatg cgatcctgcc tgcagctccc cagcctgtct
     5221 gtacgacaat ttcgattgcc acgcaggcgg cagagagcgg acctgtaatc ctgtgtacga
     5281 gaagtactgc gccgaccact tcgccgacgg aagatgtgac cagggctgca acaccgagga
     5341 atgcggctgg gatggactgg attgtgcttc cgaagttccc gctctgcttg ctagaggcgt
     5401 gctggttctg acagttctgc tgccaccaga ggaactgctg agaagcagcg ccgatttcct
     5461 gcagagactg tccgctatcc tgagaaccag cctgcggttc agactggatg ctcacggaca
     5521 ggccatggtg ttcccttacc ataggccatc tccaggcagc gagcctagag ccagaagaga
     5581 actggctcct gaagtgatcg gcagcgtcgt gatgctggaa atcgacaaca gactgtgcct
     5641 gcagagcccc gagaacgacc actgtttccc tgatgctcag agcgccgctg attatctggg
     5701 agccctgtct gctgtggaac ggctggactt tccctatcct ctgagagatg tgcggggcga
     5761 gccactggaa cctccagaac cttctgtgcc ccttggcgcg ccgggcggcg acaagaccca
     5821 cacctgcccc ccctgcccag ctccagaact gctgggaggg ccttccgtgt tcctgttccc
     5881 ccccaagccc aaggacaccc tgatgatcag caggaccccc gaggtgacct gcgtggtggt
     5941 ggccgtgtcc cacgaggacc cagaggtgaa gttcaactgg tacgtggacg gcgtggaggt
     6001 gcacaacgcc aagaccaagc ccagagagga gcagtacaac agcacctaca gggtggtgtc
     6061 cgtgctgacc gtgctgcacc aggactggct gaacggcaaa gaatacaagt gcaaagtctc
     6121 caacaaggcc ctggctgccc caatcgaaaa gacaatcagc aaggccaagg gccagccacg
     6181 ggagccccag gtgtacaccc tgccccccag ccgggaggag atgaccaaga accaggtgtc
     6241 cctgacctgt ctggtgaagg gcttctaccc cagcgatatc gccgtggagt gggagagcaa
     6301 cggccagccc gagaacaact acaagaccac ccccccagtg ctggacagcg acggcagctt
     6361 cttcctgtac agcaagctga ccgtggacaa gtccaggtgg cagcagggca acgtgttcag
     6421 ctgcagcgtg atgcacgagg ccctgcacaa ccactacacc cagaagtccc tgagcctgag
     6481 ccccggcggc agcggtctta atgatatttt cgaggctcag aagattgagt ggcatgaaca
     6541 ccaccaccat caccactaag gccggcctgt agtcactggc acaacgcgtt acccggtaag
     6601 ccaatcgggt atacacggtc gtcatactgc agacagggtt cttctacttt gcaagatagt
     6661 cttgagtagt aaaataaata gatagagaaa atcagcctcg actgtgcctt ctagttgcca
     6721 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6781 tgtcctttcc taataaaatg aggaaattgc atcacaacac tcaaccctat ctcggtctat
     6841 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6901 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     6961 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7021 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7081 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     7141 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7201 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     7261 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     7321 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     7381 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     7441 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     7501 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     7561 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     7621 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     7681 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     7741 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     7801 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     7861 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     7921 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaca
     7981 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     8041 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     8101 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8161 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8221 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8281 attaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8341 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8401 ccagcgctgc gatgataccg cgagaaccac gctcaccggc tccggattta tcagcaataa
     8461 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8521 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8581 acgttgttgc catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8641 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8701 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8761 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8821 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8881 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8941 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9001 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9061 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9121 cacggaaatg ttgaatactc atattcttcc tttttcaata ttattgaagc atttatcagg
     9181 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9241 tcagtgttac aaccaattaa ccaattctga acattatcgc gagcccattt atacctgaat
     9301 atggctcata acaccccttg ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc
     9361 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     9421 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9481 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     9541 accaaatact gttcttctag tgtagccgta gttagcccac cacttcaaga actctgtagc
     9601 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9661 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9721 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9781 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9841 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9901 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9961 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    10021 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    10081 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    10141 cgagcgcagc gagtcagtga gcgaggaagc ggaaggcgag agtagggaac tgccaggcat
    10201 caaactaagc agaaggcccc tgacggatgg cctttttgcg tttctacaaa ctctttctgt
    10261 gttgtaaaac gacggccagt cttaagctcg ggccccctgg gcggttctga taacgagtaa
    10321 tcgttaatcc gcaaataacg taaaaacccg cttcggcggg tttttttatg gggggagttt
    10381 agggaaagag catttgtcag aatatttaag ggcgcctgtc actttgcttg atatatgaga
    10441 attatttaac cttataaatg agaaaaaagc aacgcacttt aaataagata cgttgctttt
    10501 tcgattgatg aacacctata attaaactat tcatctatta tttatgattt tttgtatata
    10561 caatatttct agtttgttaa agagaattaa gaaaataaat ctcgaaaata ataaagggaa
    10621 aatcagtttt tgatatcaaa attatacatg tcaacgataa tacaaaatat aatacaaact
    10681 ataagatgtt atcagtattt attatcattt agaataaatt ttgtgtcgcc cttaattgtg
    10741 agcggataac aattacgagc ttcatgcaca gtggcgttga ctcaatattg gccattagcc
    10801 atattattca ttggttatat agcataaatc aatattggct attggccatt gcatacgttg
    10861 tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc gccatgtt
//
