LOCUS       sequence-405781-       11062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11062)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 11062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NOTCH2(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..11062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6638..6682
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6683..6700
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6840..6857)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6846..6957
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7075..7095)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7080..7409
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7260..7395
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7322..7341
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7457..7504
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7523..8122
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7523..7542)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8019..8039
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8252..8373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8289..8308)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8343..8362
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8426..9217,9218..9286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9049..9068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9287..9378)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9420..9439
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9445..9464
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9550..10138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10039..10058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10292..10309
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     10407..10424
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10408..10424
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10880..10896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccttgcagtg tagagatggc tacgagccct gcgtgaacga
     1021 agggatgtgc gtgacctatc acaacggcac cggctactgc aagtgccctg agggctttct
     1081 gggcgagtac tgccagcaca gagatccctg cgagaagaac agatgccaga acggcggaac
     1141 atgtgtggcc caggctatgc tgggcaaagc cacatgtaga tgcgccagcg gctttaccgg
     1201 cgaggattgc cagtacagca ccagccatcc ttgcttcgtg tccagacctt gcctgaatgg
     1261 cggcacctgt cacatgctga gcagagacac ctacgagtgt acctgccaag tgggcttcac
     1321 cggcaaagaa tgccagtgga ccgatgcctg cctgtctcac ccttgtgcca atggcagcac
     1381 atgcaccacc gtggccaatc agttcagctg caagtgtctg accggcttca caggccagaa
     1441 atgcgagaca gacgtgaacg agtgcgacat ccctggacac tgtcagcatg gcggaacctg
     1501 cctgaatctg cctggcagct atcagtgtca gtgcccacag ggattcaccg gccagtactg
     1561 tgatagcctg tacgtgccct gcgctccctc tccttgtgtt aacggtggca cctgtagaca
     1621 gaccggcgac ttcaccttcg agtgcaattg cctgccaggc ttcgagggct ctacctgcga
     1681 gagaaacatc gacgactgcc ccaaccacag gtgtcaaaat ggcggagtgt gtgtggacgg
     1741 cgtgaacacc tacaactgca gatgtcctcc tcagtggaca ggccagttct gcaccgagga
     1801 tgtggatgag tgcctgctgc agcctaacgc ttgtcagaat ggtggcacat gcgccaacag
     1861 aaatggcggc tatggctgcg tgtgtgtgaa tggatggtcc ggcgacgact gtagcgagaa
     1921 catcgatgat tgcgccttcg ccagctgtac ccctggcagc acctgtatcg atagagtggc
     1981 cagcttctcc tgcatgtgcc cagaaggcaa agccggactg ctgtgtcatc tggatgacgc
     2041 ctgcatcagc aacccatgtc acaaaggcgc cctgtgcgac accaatcctc tgaatggcca
     2101 gtacatctgc acctgtcctc agggctataa gggcgccgac tgtacagagg acgtggacga
     2161 atgtgccatg gccaacagca atccttgcga gcacgccggc aaatgtgtga acacagatgg
     2221 cgccttccac tgcgagtgcc tgaaaggata tgccggacct agatgcgaga tggacatcaa
     2281 cgagtgccac agcgacccct gccagaatga tgccacctgt ctggacaaga tcggcggctt
     2341 cacttgcctg tgcatgcctg gctttaaggg cgtgcactgc gagctggaaa tcaatgaatg
     2401 tcaaagcaac ccctgcgtca acaacggcca gtgcgtggac aaagtgaaca gattccagtg
     2461 cctgtgtcct cctggcttca ctggcccagt gtgccagatc gacattgacg attgcagcag
     2521 caccccttgt ctgaacggcg ccaagtgcat cgatcaccct aacggctatg agtgccagtg
     2581 cgctacaggc tttacaggcg tgctgtgcga agagaatatc gacaactgcg accccgatcc
     2641 ttgccaccac ggacagtgtc aggacggcat cgacagctac acatgcatct gcaatcctgg
     2701 ctacatggga gccatctgca gcgaccagat cgatgagtgc tacagcagcc catgcctgaa
     2761 cgacggcaga tgtatcgacc tggtcaacgg ctaccagtgc aactgtcagc ctggaaccag
     2821 cggagtgaac tgcgagatca acttcgacga ttgtgccagc aatccctgca tccacggcat
     2881 ctgcatggat ggcatcaaca gatacagctg tgtgtgcagc cccgggttta ccggccagag
     2941 atgcaacatt gatatcgacg agtgcgcctc caatccatgc cggaagggcg ctacatgcat
     3001 caatggcgtg aacggcttcc ggtgtatctg ccctgaagga cctcaccatc ctagctgcta
     3061 cagccaagtg aacgaatgcc tgagcaaccc ttgtatccac ggaaactgta caggcggcct
     3121 gagcggctac aaatgtctgt gtgatgccgg ctgggtcgga atcaactgcg aggtggacaa
     3181 gaatgagtgt ctgtctaatc cctgccaaaa cggtggaact tgcgacaacc tcgtgaatgg
     3241 ctacagatgt acctgcaaga agggattcaa ggggtacaac tgccaagtca acatagatga
     3301 gtgcgcttct aacccctgtc tcaaccaggg cacctgtttc gacgacatca gcggctatac
     3361 ctgccactgc gtgctgcctt acaccggcaa gaactgtcag acagtgctgg ccccttgcag
     3421 ccctaatcct tgtgaaaacg ccgccgtgtg caaagagagc cccaacttcg agtcttacac
     3481 atgcctgtgc gcccctggat ggcagggaca gagatgtacc atcgatattg atgagtgtat
     3541 cagcaagcct tgcatgaatc acggcctgtg ccacaacacc cagggctcct atatgtgcga
     3601 gtgtccacca ggctttagcg gcatggactg cgaggaagat attgacgact gtctggctaa
     3661 cccatgccag aatggcggct cttgtatgga cggggtcaac acttttagct gtctgtgtct
     3721 gcctgggttc accggggata agtgccagac cgatatgaat gaatgcctca gcgagccctg
     3781 taaaaacggc gggacctgca gcgattacgt gaactcttac acctgtaaat gccaggccgg
     3841 cttcgacggg gtgcactgtg aaaacaatat caatgagtgc accgagtcca gctgcttcaa
     3901 cggcggcact tgcgtggacg gcattaacag cttttcttgt ctgtgccccg tgggctttac
     3961 tggcagcttc tgtctgcacg agattaacga gtgcagctct cacccatgtc tgaatgaggg
     4021 aacctgtgtg gatggcctgg gcacctacag atgctcttgt cctctgggat acacagggaa
     4081 gaattgccag acactggtca acctgtgtag cagaagccct tgcaagaaca agggcacatg
     4141 tgtgcagaag aaagccgaga gccagtgtct ctgtccttct ggatgggctg gcgcctactg
     4201 tgatgtgccc aatgtgtctt gtgatatcgc cgccagtaga cggggagtgc tggtggaaca
     4261 tctgtgtcag cacagcggcg tgtgcatcaa cgccggaaat acccactact gccagtgtcc
     4321 actgggctat accggctcct attgcgagga acagctggac gagtgtgcaa gtaacccttg
     4381 tcagcacggg gccacctgtt ctgacttcat cggaggatac agatgcgagt gcgtgccagg
     4441 atatcagggc gtgaactgtg aatacgaagt tgacgagtgt cagaaccagc cttgccaaaa
     4501 tggcgggaca tgcatcgacc tcgtgaacca cttcaagtgc agctgccctc ctggcacaag
     4561 aggcctgctc tgtgaagaaa acattgatga ctgtgccaga ggaccccact gtctcaatgg
     4621 cggccagtgt atggatcgga ttggcggcta ctcctgtaga tgtctgccag gatttgccgg
     4681 ggagagatgc gagggcgaca ttaacgaatg tctcagtaac ccctgcagca gcgagggcag
     4741 cctggattgc atccagctga ccaacgacta cctgtgcgtg tgcagatccg cctttaccgg
     4801 aaggcactgc gaaaccttcg tggacgtgtg ccctcagatg ccttgtctta atggcggaac
     4861 ttgcgccgtg gccagcaaca tgcctgatgg ctttatctgt cgctgcccac ctggattttc
     4921 cggcgctaga tgtcaatcct cctgcggaca agtgaagtgc agaaagggcg agcagtgcgt
     4981 gcacacagcc agcggaccaa gatgcttctg cccatctcct agagattgcg agagcggctg
     5041 tgcttctagt ccctgtcaac acggcggaag ctgtcaccct cagagacagc caccttacta
     5101 cagctgtcag tgcgccccac cttttagcgg ctccagatgc gaactgtaca cagcccctcc
     5161 aagcacacct cctgccacat gtctgtccca gtattgcgcc gacaaggcaa gagatggcgt
     5221 gtgcgacgag gcctgtaatt ctcacgcatg tcaatgggat ggcggcgact gcagcctgac
     5281 catggaaaat ccttgggcca actgcagctc ccctctgcct tgctgggact acatcaacaa
     5341 ccagtgcgac gaactgtgca acaccgtgga atgcctgttc gacaatttcg agtgccaggg
     5401 caactccaag acctgcaaat acgacaagta ctgcgccgat cacttcaagg acaaccactg
     5461 cgatcagggc tgtaacagcg aggaatgtgg ctgggatgga ctggattgtg ctgccgacca
     5521 gcctgaaaat ctggccgagg gaacactcgt gatcgtggtg ctgatgcctc ctgaacagct
     5581 gctccaggac gccagatctt ttctgagagc actgggcacc ctgctgcaca ccaacctgag
     5641 aatcaagaga gactcccagg gcgagctgat ggtgtacccc tactatggcg agaagtccgc
     5701 cgccatgaag aaacagcgga tgaccagaag atctctgccc ggcgagcaag agcaagaggt
     5761 ggccggaagc aaggtgttcc tggaaatcga caaccggcag tgtgtccagg actccgacca
     5821 ctgcttcaag aacactgatg ccgctgccgc tctgctggcc tctcatgcta ttcagggcac
     5881 actgagctac cctctggtgt ctgtggtgtc cgagagcctg acacctgaga gaacccaagg
     5941 cgcgccgggc ggcgacaaga cccacacctg ccccccctgc ccagctccag aactgctggg
     6001 agggccttcc gtgttcctgt tcccccccaa gcccaaggac accctgatga tcagcaggac
     6061 ccccgaggtg acctgcgtgg tggtggccgt gtcccacgag gacccagagg tgaagttcaa
     6121 ctggtacgtg gacggcgtgg aggtgcacaa cgccaagacc aagcccagag aggagcagta
     6181 caacagcacc tacagggtgg tgtccgtgct gaccgtgctg caccaggact ggctgaacgg
     6241 caaagaatac aagtgcaaag tctccaacaa ggccctggct gccccaatcg aaaagacaat
     6301 cagcaaggcc aagggccagc cacgggagcc ccaggtgtac accctgcccc ccagccggga
     6361 ggagatgacc aagaaccagg tgtccctgac ctgtctggtg aagggcttct accccagcga
     6421 tatcgccgtg gagtgggaga gcaacggcca gcccgagaac aactacaaga ccaccccccc
     6481 agtgctggac agcgacggca gcttcttcct gtacagcaag ctgaccgtgg acaagtccag
     6541 gtggcagcag ggcaacgtgt tcagctgcag cgtgatgcac gaggccctgc acaaccacta
     6601 cacccagaag tccctgagcc tgagccccgg cggcagcggt cttaatgata ttttcgaggc
     6661 tcagaagatt gagtggcatg aacaccacca ccatcaccac taaggccggc ctgtagtcac
     6721 tggcacaacg cgttacccgg taagccaatc gggtatacac ggtcgtcata ctgcagacag
     6781 ggttcttcta ctttgcaaga tagtcttgag tagtaaaata aatagataga gaaaatcagc
     6841 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     6901 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcaca
     6961 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7021 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7081 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7141 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7201 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7261 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7321 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7381 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7441 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     7501 cgacaaggtg aggaactaaa ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     7561 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     7621 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     7681 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     7741 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     7801 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     7861 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     7921 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     7981 cgccggggtg cccgccttcc tggagacatc cgcgccccgc aacctcccct tctacgagcg
     8041 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     8101 gacccgcaag cccggtgcct gacacgtgct acgagatttc gattccaccg ccgccttcta
     8161 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     8221 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     8281 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8341 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8401 ctagagcttg gcgtaatcat ggtcattacc aatgcttaat cagtgaggca cctatctcag
     8461 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8521 tacgggaggg cttaccatct ggccccagcg ctgcgatgat accgcgagaa ccacgctcac
     8581 cggctccgga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8641 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8701 gttcgccagt taatagtttg cgcaacgttg ttgccatcgc tacaggcatc gtggtgtcac
     8761 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8821 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8881 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8941 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9001 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9061 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatattc ttcctttttc
     9301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9361 tttagaaaaa taaacaaata ggggtcagtg ttacaaccaa ttaaccaatt ctgaacatta
     9421 tcgcgagccc atttatacct gaatatggct cataacaccc cttgctcatg accaaaatcc
     9481 cttaacgtga gttacgcgcg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9541 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     9601 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9661 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagc
     9721 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9781 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     9841 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     9901 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     9961 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10021 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10081 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    10141 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    10201 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    10261 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaagg
    10321 cgagagtagg gaactgccag gcatcaaact aagcagaagg cccctgacgg atggcctttt
    10381 tgcgtttcta caaactcttt ctgtgttgta aaacgacggc cagtcttaag ctcgggcccc
    10441 ctgggcggtt ctgataacga gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg
    10501 cgggtttttt tatgggggga gtttagggaa agagcatttg tcagaatatt taagggcgcc
    10561 tgtcactttg cttgatatat gagaattatt taaccttata aatgagaaaa aagcaacgca
    10621 ctttaaataa gatacgttgc tttttcgatt gatgaacacc tataattaaa ctattcatct
    10681 attatttatg attttttgta tatacaatat ttctagtttg ttaaagagaa ttaagaaaat
    10741 aaatctcgaa aataataaag ggaaaatcag tttttgatat caaaattata catgtcaacg
    10801 ataatacaaa atataataca aactataaga tgttatcagt atttattatc atttagaata
    10861 aattttgtgt cgcccttaat tgtgagcgga taacaattac gagcttcatg cacagtggcg
    10921 ttgactcaat attggccatt agccatatta ttcattggtt atatagcata aatcaatatt
    10981 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
    11041 tgtccaatat gaccgccatg tt
//
