LOCUS       sequence-405782-       11257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11257)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 11257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NOTCH1(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..11257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6833..6877
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6878..6895
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7035..7052)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7041..7152
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7270..7290)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7275..7604
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7455..7590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7517..7536
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7652..7699
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7718..8317
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7718..7737)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8214..8234
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8447..8568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8484..8503)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8538..8557
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8621..9412,9413..9481))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9244..9263
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9482..9573)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9615..9634
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9640..9659
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9745..10333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10234..10253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10487..10504
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     10602..10619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10603..10619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11075..11091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccgcaagagg acctagatgt tctcaacctg gcgagacatg
     1021 cctgaacggc ggaaagtgtg aagccgccaa tggaaccgaa gcctgtgtgt gtggcggagc
     1081 ctttgtgggc cctagatgtc aagaccccaa tccttgtctg agcacccctt gcaagaatgc
     1141 cggcacctgt cacgtggtgg atagaagagg cgtggccgat tacgcctgta gctgtgccct
     1201 tggctttagc ggccctctgt gtctgacacc tctggacaac gcctgcctga caaacccctg
     1261 tagaaacggc ggcacatgcg atctgctgac cctgaccgag tacaagtgca gatgtcctcc
     1321 tggatggtcc ggcaagagct gtcagcaagc cgatccttgt gccagcaatc cttgcgccaa
     1381 tggcggccag tgtctgcctt ttgaggccag ctacatctgc cactgtcctc caagctttca
     1441 cggccctacc tgcagacagg atgtgaacga gtgtggccag aaacctggcc tgtgtagaca
     1501 tggcggaacc tgccacaacg aagtgggcag ctacagatgc gtgtgcagag ccactcacac
     1561 cggacctaat tgcgagaggc cttacgtgcc atgcagccca tcaccttgtc agaacggcgg
     1621 gacttgtaga cctaccggcg acgtgacaca cgagtgtgct tgtctgcctg gcttcaccgg
     1681 ccagaactgc gaggaaaaca tcgacgactg ccccggcaac aactgcaaaa atggcggagc
     1741 atgtgtggac ggcgtgaaca cctacaactg tagatgccct ccagagtgga caggccagta
     1801 ctgcacagag gatgtggatg agtgccagct gatgcccaat gcctgccaga atggcggcac
     1861 ttgccataat acccacggcg gctacaactg cgtgtgtgtg aatggatgga ccggcgagga
     1921 ctgctccgag aacatcgatg attgtgcctc cgccgcctgt ttccacggcg ccacatgtca
     1981 tgatagagtg gccagcttct actgcgagtg ccctcacgga agaacaggcc tgctgtgtca
     2041 cctgaacgac gcctgcatca gcaatccctg caacgagggc agcaactgcg acaccaatcc
     2101 tgtgaacggc aaggccatct gtacatgccc tagcggctat acaggacctg cctgtagtca
     2161 ggacgtggac gaatgttctc tgggcgccaa tccatgcgag cacgccggca aatgtatcaa
     2221 caccctgggc agcttcgagt gccagtgcct gcaaggatat acaggcccca gatgcgagat
     2281 cgatgtgaat gaatgcgtca gcaacccttg ccagaacgat gccacctgtc tggaccagat
     2341 cggcgagttc cagtgcatct gtatgcctgg ctatgagggc gtgcactgcg aagtgaatac
     2401 cgacgaatgc gccagctctc cctgcctgca caatggcaga tgcctggaca agatcaatga
     2461 gtttcagtgc gagtgtccca ccggctttac aggccacctg tgccagtatg atgtcgacga
     2521 gtgcgcctcc acaccttgca aaaacggcgc caagtgcctg gacggcccca atacctacac
     2581 ttgtgtgtgc accgagggct acaccggaac acactgcgag gtggacattg atgagtgcga
     2641 ccccgatcca tgtcactacg gcagctgtaa agacggcgtg gcaaccttca catgcctgtg
     2701 cagacctggc tatactggcc accactgcga gactaacatc aacgagtgta gcagccagcc
     2761 atgcaggcac ggtggaacct gtcaggacag agacaatgcc tacctgtgct tctgcctgaa
     2821 gggcaccacc gggcctaact gtgaaatcaa cctggacgac tgcgcaagca gcccttgcga
     2881 ttctggcact tgtctggata agatcgacgg ctatgagtgt gcctgcgagc ctggatacac
     2941 cggcagcatg tgcaacatca atatcgatga atgcgctggc aacccatgtc acaatggcgg
     3001 gacctgtgaa gatggcatca acggcttcac ctgtcggtgc cctgagggct accatgatcc
     3061 tacctgtctg tccgaagtga acgaatgcaa tagcaacccc tgcgtgcacg gcgcctgcag
     3121 agatagcctg aatggctaca agtgcgactg cgatccaggc tggtccggga ccaactgcga
     3181 tatcaacaac aacgaatgcg agtctaaccc ttgcgtcaac ggtggcactt gcaaggacat
     3241 gaccagcggc tacgtgtgca cctgtagaga gggcttctct ggccccaact gccagaccaa
     3301 tattaacgaa tgtgcttcta acccctgcct gaaccagggc acctgtatcg atgatgtggc
     3361 cggctataag tgcaactgtc tgctgcctta caccggggcc acatgtgaag tggttctggc
     3421 cccttgtgca ccctctccat gtcgtaatgg cggcgaatgc agacagagcg aggactacga
     3481 gagcttcagc tgtgtgtgcc caacaggctg gcagggacag acatgcgaag tggatatcaa
     3541 tgaatgtgtg ctgagcccct gcaggcatgg cgccagctgt cagaatactc atggcggcta
     3601 tagatgccac tgccaggccg gctacagcgg cagaaattgc gaaaccgata tcgacgattg
     3661 ccggcctaat ccatgccata acggcggctc ttgcaccgac ggcatcaaca ccgccttttg
     3721 cgattgcctg cctggattca gaggcacctt ctgcgaagag gacattaacg agtgtgcctc
     3781 cgatccttgc cggaatggcg ccaattgcac cgattgcgtg gacagctaca cctgtacctg
     3841 tcctgccggc ttttccggca tccactgcga aaacaacacc cctgactgca ccgagtccag
     3901 ctgcttcaat ggtggcacct gtgtggatgg gattaactcc tttacatgtc tgtgcccacc
     3961 tgggtttacc ggcagctact gtcagcacga tgtcaatgag tgtgactccc agccttgtct
     4021 gcacggcggt acatgtcaag atggctgcgg ctcctaccgg tgtacatgtc ctcaaggcta
     4081 cacagggccc aactgtcaga acctggtgca ttggtgcgac agcagcccat gcaagaatgg
     4141 cggaaagtgc tggcagaccc acacacagta tagatgcgaa tgcccttctg gctggaccgg
     4201 cctgtattgt gatgtgcctt ctgtgtcttg cgaggtcgcc gctcaaagac agggtgtcga
     4261 tgtggctaga ctgtgtcaac acggtggact gtgcgtggac gctggcaata cccatcattg
     4321 cagatgccag gctgggtaca caggcagcta ttgcgaggat ctggtggacg agtgctcccc
     4381 atctccatgc caaaatggcg ctacctgtac cgactacctc ggcggataca gctgcaaatg
     4441 tgtggccgga taccatggcg tgaactgcag cgaagagatt gacgagtgcc tgtctcaccc
     4501 ctgccaaaac ggtggtacat gcctggatct gcccaacaca tacaagtgct cttgccctag
     4561 aggcacccag ggtgtccatt gcgaaatcaa tgttgacgac tgcaaccctc ctgtggaccc
     4621 cgtgtccaga tctcccaagt gcttcaacaa tgggacctgc gtggaccaag tcggcggcta
     4681 ttcttgtact tgccctcctg gcttcgtggg cgagagatgt gaaggcgacg tcaacgaatg
     4741 tttgtctaat ccctgtgacg ccaggggcac ccagaattgc gtgcagagag tgaacgactt
     4801 ccactgtgaa tgcagagccg gacacactgg gagaagatgc gagtccgtga tcaatggctg
     4861 caagggcaaa ccatgtaaaa acggcggaac ttgcgccgtg gcctctaata ccgctagagg
     4921 cttcatctgc aagtgtcccg ccggatttga gggcgccact tgcgaaaatg atgccagaac
     4981 ctgcggcagc ctgagatgcc ttaatggtgg aacatgcatc agcggcccta gaagccctac
     5041 ttgcctgtgt ctgggccctt tcacaggccc cgagtgtcag tttcctgcca gttctccttg
     5101 tctcggcgga aacccctgct acaatcaggg aacatgcgag cctaccagcg agagcccctt
     5161 ctacagatgt ctgtgtcctg ccaagttcaa cggactgctg tgccacatcc tggattacag
     5221 ctttggaggc ggcgctggca gagacatccc tccacctctt attgaggaag cctgcgaact
     5281 gcccgagtgc caagaggatg ctggcaacaa agtgtgcagc ctgcagtgca acaatcacgc
     5341 ctgtggctgg gatggcggag actgctccct gaacttcaac gacccctgga agaactgcac
     5401 ccagtctctg cagtgctgga agtactttag cgacggccac tgcgacagcc agtgtaattc
     5461 tgccggctgc ctgttcgacg gcttcgattg tcagcgagct gagggccagt gcaatcccct
     5521 gtacgaccag tattgcaagg atcacttctc cgatggccac tgtgaccagg gctgtaatag
     5581 cgccgaatgc gaatgggatg gcctggattg tgccgagcac gtgccagaaa gactggctgc
     5641 tggaacactg gtggtggtcg tgctgatgcc tcctgagcag ctgagaaaca gcagcttcca
     5701 cttcctgcgc gagctgtcca gagtgctgca caccaatgtg gtgttcaaga gagatgccca
     5761 cggccagcag atgatcttcc catattacgg cagagaagag gaactgcgga agcaccctat
     5821 caagagagct gctgaaggct gggctgctcc tgatgctctg ctgggacaag tgaaagcctc
     5881 tctgcttcct ggcggaagcg aaggcggaag gcggagaaga gaactcgatc ctatggatgt
     5941 gcggggcagc atcgtgtacc tggaaatcga caacagacag tgcgtgcagg cctccagcca
     6001 atgttttcag tctgctaccg acgtggccgc ctttctggga gcacttgctt ccctgggatc
     6061 tctgaacatc ccctacaaga tcgaggccgt gcagtccgag acagtggaac ctcctcctcc
     6121 tgctcaactg catggcgcgc cgggcggcga caagacccac acctgccccc cctgcccagc
     6181 tccagaactg ctgggagggc cttccgtgtt cctgttcccc cccaagccca aggacaccct
     6241 gatgatcagc aggacccccg aggtgacctg cgtggtggtg gccgtgtccc acgaggaccc
     6301 agaggtgaag ttcaactggt acgtggacgg cgtggaggtg cacaacgcca agaccaagcc
     6361 cagagaggag cagtacaaca gcacctacag ggtggtgtcc gtgctgaccg tgctgcacca
     6421 ggactggctg aacggcaaag aatacaagtg caaagtctcc aacaaggccc tggctgcccc
     6481 aatcgaaaag acaatcagca aggccaaggg ccagccacgg gagccccagg tgtacaccct
     6541 gccccccagc cgggaggaga tgaccaagaa ccaggtgtcc ctgacctgtc tggtgaaggg
     6601 cttctacccc agcgatatcg ccgtggagtg ggagagcaac ggccagcccg agaacaacta
     6661 caagaccacc cccccagtgc tggacagcga cggcagcttc ttcctgtaca gcaagctgac
     6721 cgtggacaag tccaggtggc agcagggcaa cgtgttcagc tgcagcgtga tgcacgaggc
     6781 cctgcacaac cactacaccc agaagtccct gagcctgagc cccggcggca gcggtcttaa
     6841 tgatattttc gaggctcaga agattgagtg gcatgaacac caccaccatc accactaagg
     6901 ccggcctgta gtcactggca caacgcgtta cccggtaagc caatcgggta tacacggtcg
     6961 tcatactgca gacagggttc ttctactttg caagatagtc ttgagtagta aaataaatag
     7021 atagagaaaa tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     7081 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     7141 ggaaattgca tcacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7201 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7261 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     7321 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7381 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7441 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7501 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     7561 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     7621 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     7681 cggcatagta taatacgaca aggtgaggaa ctaaaccatg accgagtaca agcccacggt
     7741 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     7801 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     7861 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     7921 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     7981 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     8041 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     8101 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     8161 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acatccgcgc cccgcaacct
     8221 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     8281 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacac gtgctacgag atttcgattc
     8341 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8401 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8461 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8521 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     8581 accgtcgacc tctagctaga gcttggcgta atcatggtca ttaccaatgc ttaatcagtg
     8641 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8701 tgtagataac tacgatacgg gagggcttac catctggccc cagcgctgcg atgataccgc
     8761 gagaaccacg ctcaccggct ccggatttat cagcaataaa ccagccagcc ggaagggccg
     8821 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8881 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc atcgctacag
     8941 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9001 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9061 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9121 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9181 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9241 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9301 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9361 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9421 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9481 tattcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9541 acatatttga atgtatttag aaaaataaac aaataggggt cagtgttaca accaattaac
     9601 caattctgaa cattatcgcg agcccattta tacctgaata tggctcataa caccccttgc
     9661 tcatgaccaa aatcccttaa cgtgagttac gcgcgcgtcg ttccactgag cgtcagaccc
     9721 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9781 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9841 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9901 gtagccgtag ttagcccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9961 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10021 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10081 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10141 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10201 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10261 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10321 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10381 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    10441 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    10501 cgaggaagcg gaaggcgaga gtagggaact gccaggcatc aaactaagca gaaggcccct
    10561 gacggatggc ctttttgcgt ttctacaaac tctttctgtg ttgtaaaacg acggccagtc
    10621 ttaagctcgg gccccctggg cggttctgat aacgagtaat cgttaatccg caaataacgt
    10681 aaaaacccgc ttcggcgggt ttttttatgg ggggagttta gggaaagagc atttgtcaga
    10741 atatttaagg gcgcctgtca ctttgcttga tatatgagaa ttatttaacc ttataaatga
    10801 gaaaaaagca acgcacttta aataagatac gttgcttttt cgattgatga acacctataa
    10861 ttaaactatt catctattat ttatgatttt ttgtatatac aatatttcta gtttgttaaa
    10921 gagaattaag aaaataaatc tcgaaaataa taaagggaaa atcagttttt gatatcaaaa
    10981 ttatacatgt caacgataat acaaaatata atacaaacta taagatgtta tcagtattta
    11041 ttatcattta gaataaattt tgtgtcgccc ttaattgtga gcggataaca attacgagct
    11101 tcatgcacag tggcgttgac tcaatattgg ccattagcca tattattcat tggttatata
    11161 gcataaatca atattggcta ttggccattg catacgttgt atctatatca taatatgtac
    11221 atttatattg gctcatgtcc aatatgaccg ccatgtt
//
