LOCUS       sequence-405784-        6163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing dominant negative HIF1A in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Xue Y, Wang SK, Rana P, West ER, Hong CM, Feng H, Wu DM, Cepko CL
  TITLE     AAV-Txnip prolongs cone survival and vision in mouse models of 
            retinitis pigmentosa.
  JOURNAL   Elife. 2021 Apr 13;10. pii: 66240. doi: 10.7554/eLife.66240.
  PUBMED    33847261
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-04-13"; volume: "10. pii: 66240. doi"; pages: " 
            10.7554/eLife.66240"
COMMENT     Sequence Label: AAV-RO1.7-dnHIF
FEATURES             Location/Qualifiers
     source          1..6163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     complement(3046..3066)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   3426..3566
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3426..3555
                     /label=AAV2 ITR
     rep_origin      3641..4096
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3728..3747)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3938..3959
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4113..4132)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4232..4254
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4292..4310)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4378..4482
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4483..4551,4552..5343)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4701..4720)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5514..6102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6003..6022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcaa atagaagggt gggtgcagga ggctgagggg tggggaaagg
      181 gcatgggtgt ttcatgagga cagagcttcc gtttcatgca atgaaaagag tttggagacg
      241 gatggtggtg actggactat acacttacac acggtagcga tggtacactt tgtattatgt
      301 atattttacc acgatctttt taaagtgtca aaggcaaatg gccaaatggt tccttgtcct
      361 atagctgtag cagccatcgg ctgttagtga caaagcccct gagtcaagat gacagcagcc
      421 cccataactc ctaatcggct ctcccgcgtg gagtcattta ggagtagtcg cattagagac
      481 aagtccaaca tctaatcttc caccctggcc agggccccag ctggcagcga gggtgggaga
      541 ctccgggcag agcagagggc gctgacattg gggcccggcc tggcttgggt ccctctggcc
      601 tttccccagg ggccctcttt ccttggggct ttcttgggcc gccactgctc ccgctcctct
      661 ccccccatcc caccccctca ccccctcgtt cttcatatcc ttctctagtg ctccctccac
      721 tttcatccac ccttctgcaa gagtgtggga ccacaaatga gttttcacct ggcctgggga
      781 cacacgtgcc cccacaggtg ctgagtgact ttctaggaca gtaatctgct ttaggctaaa
      841 atgggacttg atcttctgtt agccctaatc atcaattagc agagccggtg aaggtgcaga
      901 acctaccgcc tttccaggcc tcctcccacc tctgccacct ccactctcct tcctgggatg
      961 tgggggctgg cacacgtgtg gcccagggca ttggtgggat tgcactgagc tgggtcatta
     1021 gcgtaatcct ggacaagggc agacagggcg agcggagggc cagctccggg gctcaggcaa
     1081 ggctgggggc ttcccccaga caccccactc ctcctctgct ggacccccac ttcatagggc
     1141 acttcgtgtt ctcaaagggc ttccaaatag catggtggcc ttggatgccc agggaagcct
     1201 cagagttgct tatctccctc tagacagaag gggaatctcg gtcaagaggg agaggtcgcc
     1261 ctgttcaagg ccacccagcc agctcatggc ggtaatggga caaggctggc cagccatccc
     1321 accctcagaa gggacccggt ggggcaggtg atctcagagg aggctcactt ctgggtctca
     1381 cattcttgga tccggttcca ggcctcggcc ctaaatagtc tccctgggct ttcaagagaa
     1441 ccacatgaga aaggaggatt cgggctctga gcagtttcac cacccacccc ccagtctgca
     1501 aatcctgacc cgtgggtcca cctgccccaa aggcggacgc aggacagtag aagggaacag
     1561 agaacacata aacacagaga gggccacagc ggctcccaca gtcaccgcca ccttcctggc
     1621 ggggatgggt ggggcgtctg agtttggttc ccagcaaatc cctctgagcc gcccttgcgg
     1681 gctcgcctca ggagcagggg agcaagaggt gggaggagga ggtctaagtc ccaggcccaa
     1741 ttaagagatc aggtagtgta gggtttggga gcttttaagg tgaagaggcc cgggctgatc
     1801 ccacaggcca gtataaagcg ccgtgaccct caggtgatgc gccagggccg gctgccgtcg
     1861 gggacagggc tttccatagc atatgtctcg gcgaagcaaa gagtctgaag ttttttatga
     1921 gcttgctcat cagttgccac ttccccacaa tgtgagctca catcttgata aagcttctgt
     1981 tatgaggctc accatcagtt atttacgtgt gagaaaactt ctggatgccg gtggtctaga
     2041 cagtgaagat gagatgaagg cacagatgga ctgtttttat ctgaaagccc tagatggctt
     2101 tgtgatggtg ctaacagatg acggcgacat ggtttacatt tctgataacg tgaacaaata
     2161 catggggtta actcagtttg aactaactgg acacagtgtg tttgatttta ctcatccatg
     2221 tgaccatgag gaaatgagag aaatgcttac acacagaaat ggcccagtga gaaaagggaa
     2281 agaactaaac acacagcgga gcttttttct cagaatgaag tgcaccctaa caagccgggg
     2341 gaggacgatg aacatcaagt cagcaacgtg gaaggtgctt cactgcacgg gccatattca
     2401 tgtctatgat accaacagta accaacctca gtgtgggtac aagaaaccac ccatgacgtg
     2461 cttggtgctg atttgtgaac ccattcctca tccgtcaaat attgaaattc ctttagacag
     2521 caagacattt ctcagtcgac acagcctcga tatgaaattt tcttactgtg atgaaagaat
     2581 tactgagttg atgggttatg agccggaaga acttttgggc cgctcaattt atgaatatta
     2641 tcatgctttg gattctgatc atctgaccaa aactcaccat gatatgttta ctaaaggaca
     2701 agtcaccaca ggacagtaca ggatgcttgc caaaagaggt ggatatgtct gggttgaaac
     2761 tcaagcaact gtcatatata atacgaagaa ctcccagcca cagtgcattg tgtgtgtgaa
     2821 ttatgttgta agtggtatta ttcagcacga cttgattttc tcccttcaac aaacagaatc
     2881 tgtgctcaaa ccagttgaat cttcagatat gaagatgact cagctgttca ccaaagttga
     2941 atcagaggat acaagctgcc tttttgataa gcttaagtga cccgctcgag ataatcaacc
     3001 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3061 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3121 cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc
     3181 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3241 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atctagcttt atttgtgaaa
     3301 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     3361 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagcg
     3421 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     3481 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     3541 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
     3601 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
     3661 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
     3721 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3781 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3841 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3901 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3961 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     4021 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4081 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     4141 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     4201 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     4261 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     4321 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     4381 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4441 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4501 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4561 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4621 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4681 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4741 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4801 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4861 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4921 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4981 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5041 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5101 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5161 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5221 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5281 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5341 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5401 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5461 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5521 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5581 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5641 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5701 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5761 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5821 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5881 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5941 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6001 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6061 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6121 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
