LOCUS       sequence-405786-        7275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing mutant TXNIP in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Xue Y, Wang SK, Rana P, West ER, Hong CM, Feng H, Wu DM, Cepko CL
  TITLE     AAV-Txnip prolongs cone survival and vision in mouse models of 
            retinitis pigmentosa.
  JOURNAL   Elife. 2021 Apr 13;10. pii: 66240. doi: 10.7554/eLife.66240.
  PUBMED    33847261
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-04-13"; volume: "10. pii: 66240. doi"; pages: " 
            10.7554/eLife.66240"
COMMENT     Sequence Label: AAV-RedO-Txnip(C247S)
FEATURES             Location/Qualifiers
     source          1..7275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   259..388
                     /label=AAV2 ITR
     primer_bind     complement(3786..3806)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4204..4215)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4282..4299)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4288..4495
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4583..4712
                     /label=AAV2 ITR
     primer_bind     complement(4731..4748)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4731..4747)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4740..4762)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4889..5344
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4976..4995)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5186..5207
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5370..5474
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5475..5543,5544..6335)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5693..5712)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6506..7094
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6995..7014
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagatcgga attcgccctt aagctagcct acagcagcca
      481 gggtgagatt atgaggctga gctgagaata tcaagactgt accgagtagg gggccttggc
      541 aagtgtggag agcccggcag ctggggcaga gggcggagta cggtgtgcgt ttacggacct
      601 cttcaaacga ggtaggaagg tcagaagtca aaaagggaac aaatgatgtt taaccacaca
      661 aaaatgaaaa tccaatggtt ggatatccat tccaaataca caaaggcaac ggataagtga
      721 tccgggccag gcacagaagg ccatgcaccc gtaggattgc actcagagct cccaaatgca
      781 taggaataga agggtgggtg caggaggctg aggggtgggg aaagggcatg ggtgtttcat
      841 gaggacagag cttccgtttc atgcaatgaa aagagtttgg agacggatgg tggtgactgg
      901 actatacact tacacacggt agcgatggta cactttgtat tatgtatatt ttaccacgat
      961 ctttttaaag tgtcaaaggc aaatggccaa atggttcctt gtcctatagc tgtagcagcc
     1021 atcggctgtt agtgacaaag cccctgagtc aagatgacag cagcccccat aactcctaat
     1081 cggctctccc gcgtggagtc atttaggagt agtcgcatta gagacaagtc caacatctaa
     1141 tcttccaccc tggccagggc cccagctggc agcgagggtg ggagactccg ggcagagcag
     1201 agggcgctga cattggggcc cggcctggct tgggtccctc tggcctttcc ccaggggccc
     1261 tctttccttg gggctttctt gggccgccac tgctcccgct cctctccccc catcccaccc
     1321 cctcaccccc tcgttcttca tatccttctc tagtgctccc tccactttca tccacccttc
     1381 tgcaagagtg tgggaccaca aatgagtttt cacctggcct ggggacacac gtgcccccac
     1441 aggtgctgag tgactttcta ggacagtaat ctgctttagg ctaaaatggg acttgatctt
     1501 ctgttagccc taatcatcaa ttagcagagc cggtgaaggt gcagaaccta ccgcctttcc
     1561 aggcctcctc ccacctctgc cacctccact ctccttcctg ggatgtgggg gctggcacac
     1621 gtgtggccca gggcattggt gggattgcac tgagctgggt cattagcgta atcctggaca
     1681 agggcagaca gggcgagcgg agggccagct ccggggctca ggcaaggctg ggggcttccc
     1741 ccagacaccc cactcctcct ctgctggacc cccacttcat agggcacttc gtgttctcaa
     1801 agggcttcca aatagcatgg tggccttgga tgcccaggga agcctcagag ttgcttatct
     1861 ccctctagac agaaggggaa tctcggtcaa gagggagagg tcgccctgtt caaggccacc
     1921 cagccagctc atggcggtaa tgggacaagg ctggccagcc atcccaccct cagaagggac
     1981 ccggtggggc aggtgatctc agaggaggct cacttctggg tctcacattc ttggatccgg
     2041 ttccaggcct cggccctaaa tagtctccct gggctttcaa gagaaccaca tgagaaagga
     2101 ggattcgggc tctgagcagt ttcaccaccc accccccagt ctgcaaatcc tgacccgtgg
     2161 gtccacctgc cccaaaggcg gacgcaggac agtagaaggg aacagagaac acataaacac
     2221 agagagggcc acagcggctc ccacagtcac cgccaccttc ctggcgggga tgggtggggc
     2281 gtctgagttt ggttcccagc aaatccctct gagccgccct tgcgggctcg cctcaggagc
     2341 aggggagcaa gaggtgggag gaggaggtct aagtcccagg cccaattaag agatcaggta
     2401 gtgtagggtt tgggagcttt taaggtgaag aggcccgggc tgatcccaca ggccagtata
     2461 aagcgccgtg accctcaggt gatgcgccag ggccggctgc cgtcggggac agggctttcc
     2521 atagcggtac catggtgatg ttcaagaaga tcaagtcttt tgaggtggtc ttcaacgacc
     2581 ccgagaaagt gtacggcagc ggggagaagg tggccggacg ggtaattgtg gaagtgtgtg
     2641 aagttacccg agtcaaagct gtcaggatcc tggcttgcgg cgtggccaag gtcctgtgga
     2701 tgcaagggtc tcagcagtgc aaacagactt tggactactt gcgctatgaa gacacacttc
     2761 tcctagaaga gcagcctaca gcaggtgaga acgagatggt gatcatgagg cctggaaaca
     2821 aatatgagta caagttcggc ttcgagcttc ctcaagggcc cctgggaaca tcctttaaag
     2881 gaaaatatgg ttgcgtagac tactgggtga aggcttttct cgatcgcccc agccagccaa
     2941 ctcaagaggc aaagaaaaac ttcgaagtga tggatctagt ggatgtcaat acccctgact
     3001 taatggcacc agtgtctgcc aaaaaggaga agaaagtttc ctgcatgttc attcctgatg
     3061 gacgtgtgtc agtctctgct cgaattgacc gaaaaggatt ctgtgaaggt gatgacatct
     3121 ccatccatgc tgactttgag aacacgtgtt cccgaatcgt ggtccccaaa gcggctattg
     3181 tggcccgaca cacttacctt gccaatggcc agaccaaagt gttcactcag aagctgtcct
     3241 cggtcagagg caatcacatt atctcaggga ctagcgcatc gtggcgtggc aagagcctca
     3301 gagtgcagaa gatcagacca tccatcctgg gctgcaacat cctcaaagtc gaatactcct
     3361 tgctgatcta cgtcagtgtc cctggctcca agaaagtcat ccttgatctg cccctagtga
     3421 ttggcagcag gtctggtctg agcagccgga catccagcat ggccagccgg acgagctctg
     3481 agatgagctg gatagaccta aacatcccag ataccccaga agctcctcct tgctatatgg
     3541 acatcattcc tgaagatcac agactagaga gccccaccac ccctctgctg gacgatgtgg
     3601 acgactctca agacagccct atctttatgt acgcccctga gttccagttc atgcccccac
     3661 ccacttacac tgaggtggat ccgtgcgtcc ttaacaacaa caacaacaac aacaacgtgc
     3721 agtagtggat ccaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3781 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3841 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3901 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     3961 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4021 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4081 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     4141 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4201 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4261 cttccgcgtc ttcgagatct gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4321 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4381 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4441 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggactcga
     4501 gttaagggcg aattcccgat aaggatcttc ctagagcatg gctacgtaga taagtagcat
     4561 ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac tccctctctg
     4621 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     4681 cgggcggcct cagtgagcga gcgagcgcgc agccttaatt aacctaattc actggccgtc
     4741 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4801 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4861 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     4921 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4981 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5041 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5101 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5161 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5221 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5281 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5341 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5401 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5461 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5521 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5581 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5641 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5701 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5761 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5821 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5881 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5941 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6001 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6061 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6121 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6181 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6241 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6301 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6361 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6421 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6481 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6541 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6601 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6661 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6721 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6781 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6841 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6901 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6961 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7021 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7081 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7141 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7201 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7261 gcgaggaagc ggaag
//
