LOCUS       sequence-405791-        9409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-L1CAM-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..9409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4985..5029
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             5030..5047
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5187..5204)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5193..5304
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5422..5442)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5427..5756
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5607..5742
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5669..5688
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5804..5851
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5870..6469
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5870..5889)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6366..6386
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6599..6720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6636..6655)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6690..6709
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(6773..7564,7565..7633))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7396..7415
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7634..7725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7767..7786
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7792..7811
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7897..8485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8386..8405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8639..8656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8754..8771
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8755..8771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9227..9243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccattcagat ccctgaggaa tatgagggcc accacgtgat
     1021 ggaacctcca gtgattaccg agcagagccc tcggagactg gtggtgttcc ctaccgacga
     1081 tatcagcctg aagtgcgagg cctctggaaa gcccgaggtg cagttcagat ggaccagaga
     1141 tggcgtgcac ttcaagccca aagaggaact gggcgtcacc gtgtatcaga gcccacacag
     1201 cggcagcttt accatcaccg gcaacaacag caacttcgcc cagcggttcc agggcatcta
     1261 cagatgcttt gccagcaaca agctgggcac cgccatgagc cacgagatta gacttatggc
     1321 cgaaggcgcc cctaagtggc ccaaagaaac agtgaagccc gtggaagtgg aagagggcga
     1381 atctgtggtg ctgccctgta atcctcctcc ttccgccgaa cctctgcgga tctactggat
     1441 gaacagcaag atcctgcaca tcaagcagga cgagcgcgtg acaatgggcc agaacggcaa
     1501 cctgtacttc gccaacgtgc tgaccagcga caaccacagc gattacatct gccacgctca
     1561 cttccccggc accagaacca tcatccagaa agagcccatc gacctgagag tgaaggccac
     1621 caacagcatg atcgacagaa agcccagact gctgttcccc accaatagca gctctcacct
     1681 ggtggctctg cagggacaac ctctggtgct ggaatgtatc gccgagggct tccccacacc
     1741 taccattaag tggctgaggc cttctggccc catgcctgcc gatagagtga cctaccagaa
     1801 tcacaacaag accctgcagc tgctgaaagt gggcgaagag gacgatggcg agtacagatg
     1861 tctggccgag aatagcctgg gaagcgccag acacgcctac tacgtgacag tggaagccgc
     1921 tccttactgg ctgcacaagc ctcagtctca cctgtatgga cctggcgaga cagccagact
     1981 ggattgtcag gttcagggca gacctcagcc agaagtgact tggagaatca acggcatccc
     2041 tgtggaagaa ctggccaagg accagaagta cagaatccag agaggcgccc tgatcctgag
     2101 caacgtgcag cctagcgata ccatggtcac ccagtgcgaa gccagaaaca gacatggcct
     2161 gctgctggcc aacgcctaca tctatgttgt gcagctgccc gccaagattc tgaccgccga
     2221 caatcagacc tatatggccg tgcagggaag caccgcctac ctgctgtgta aagcttttgg
     2281 cgcccctgtg cctagcgtgc agtggctgga tgaagatggc accacagtgc tgcaggatga
     2341 gagattcttc ccctacgcca atggcaccct gggcattaga gatctgcagg ccaacgatac
     2401 cggcagatac ttctgcctgg ccgccaacga tcagaacaac gtgaccatca tggccaacct
     2461 gaaagtgaaa gacgccacgc agatcacaca gggccctaga agcaccatcg agaagaaagg
     2521 cagcagagtc accttcacct gtcaagccag cttcgaccct agcctgcagc cttctattac
     2581 ttggagaggc gacggcaggg acctgcaaga gctgggcgat agcgacaagt acttcatcga
     2641 ggacggccgg ctggtcatcc acagcctgga ttacagcgac cagggcaatt acagctgtgt
     2701 ggccagcacc gagctggacg ttgtggaaag tagagcacag ctgctggttg tgggaagccc
     2761 aggacctgtt cctagactgg tgctgagcga tctgcatctg ctgacacaga gccaagtgcg
     2821 agtgtcttgg agccctgccg aagatcacaa cgcccctatc gagaagtacg acatcgagtt
     2881 cgaggacaaa gagatggccc ctgagaagtg gtacagcctg ggcaaagtgc ccggcaacca
     2941 gacctctaca acactgaagc tgagccccta cgtgcactac accttcagag tgaccgccat
     3001 caacaaatac ggccctggcg agccttctcc agtgtccgaa acagtggtta cacctgaggc
     3061 cgctccagag aagaaccccg tggatgtgaa aggcgagggc aacgaaacca ccaacatggt
     3121 catcacctgg aagcccctgc ggtggatgga ttggaatgcc cctcaggtgc agtacagggt
     3181 tcagtggcga ccacagggaa caagaggacc ctggcaagag cagatcgtgt ccgatccttt
     3241 cctggtcgtg tccaacacca gcaccttcgt gccctacgag attaaggtgc aggccgtgaa
     3301 ttcccaaggc aagggacctg agcctcaagt gacaatcggc tacagcggcg aggactaccc
     3361 tcaggccatt cctgagctgg aaggcatcga gatcctgaac tctagcgccg tgcttgtgaa
     3421 gtggcggcct gtggatctgg cccaagtgaa aggacacctg agaggctaca atgtgaccta
     3481 ttggagagag ggcagccagc ggaagcacag caagagacac atccacaagg atcatgtggt
     3541 ggtgcctgcc aacaccacct ccgtgattct gagcggcctg aggccataca gcagctacca
     3601 tctggaagtg caggctttca acggcagagg ctctggccct gccagcgagt ttacattttc
     3661 tacccctgag ggcgtgccag gacaccctga agcactgcat ctggaatgcc agagcaacac
     3721 ctctctgctg ctgagatggc agcctcctct gtctcacaat ggcgtgctga caggctacgt
     3781 gctgagctat caccctctgg acgaaggcgg aaagggccag ctgagcttca atctgagaga
     3841 tcccgagctg cggacccaca acctgacaga tttgtctccc cacctgagat accggttcca
     3901 gctgcaagcc actaccaaag aaggaccagg cgaggccatc gtcagagaag gtggaacaat
     3961 ggccctgagc ggcatcagcg actttggcaa tatttctgcc actgccggcg agaactacag
     4021 cgtggtgtct tgggttccaa aagagggcca gtgcaacttc cggttccaca tcctgtttaa
     4081 ggccctggga gaagagaaag gcggagccag tctgagccct cagtacgtgt cctacaacca
     4141 gagcagctac acccagtggg atctgcagcc tgacaccgac tacgagatcc acctgttcaa
     4201 agaacggatg ttcagacacc agatggccgt caagaccaac ggaactggca gagttagact
     4261 gcctcctgcc ggctttgcca ccgaaggcgc gccgggcggc gacaagaccc acacctgccc
     4321 cccctgccca gctccagaac tgctgggagg gccttccgtg ttcctgttcc cccccaagcc
     4381 caaggacacc ctgatgatca gcaggacccc cgaggtgacc tgcgtggtgg tggccgtgtc
     4441 ccacgaggac ccagaggtga agttcaactg gtacgtggac ggcgtggagg tgcacaacgc
     4501 caagaccaag cccagagagg agcagtacaa cagcacctac agggtggtgt ccgtgctgac
     4561 cgtgctgcac caggactggc tgaacggcaa agaatacaag tgcaaagtct ccaacaaggc
     4621 cctggctgcc ccaatcgaaa agacaatcag caaggccaag ggccagccac gggagcccca
     4681 ggtgtacacc ctgcccccca gccgggagga gatgaccaag aaccaggtgt ccctgacctg
     4741 tctggtgaag ggcttctacc ccagcgatat cgccgtggag tgggagagca acggccagcc
     4801 cgagaacaac tacaagacca cccccccagt gctggacagc gacggcagct tcttcctgta
     4861 cagcaagctg accgtggaca agtccaggtg gcagcagggc aacgtgttca gctgcagcgt
     4921 gatgcacgag gccctgcaca accactacac ccagaagtcc ctgagcctga gccccggcgg
     4981 cagcggtctt aatgatattt tcgaggctca gaagattgag tggcatgaac accaccacca
     5041 tcaccactaa ggccggcctg tagtcactgg cacaacgcgt tacccggtaa gccaatcggg
     5101 tatacacggt cgtcatactg cagacagggt tcttctactt tgcaagatag tcttgagtag
     5161 taaaataaat agatagagaa aatcagcctc gactgtgcct tctagttgcc agccatctgt
     5221 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5281 ctaataaaat gaggaaattg catcacaaca ctcaacccta tctcggtcta ttcttttgat
     5341 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5401 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     5461 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     5521 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5581 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     5641 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     5701 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     5761 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     5821 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tgaccgagta
     5881 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     5941 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     6001 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6061 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6121 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6181 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6241 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6301 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacatccgc
     6361 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6421 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac acgtgctacg
     6481 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     6541 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     6601 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6661 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6721 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt cattaccaat
     6781 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6841 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagcgctg
     6901 cgatgatacc gcgagaacca cgctcaccgg ctccggattt atcagcaata aaccagccag
     6961 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7021 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7081 ccatcgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7141 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7201 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7261 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7321 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7381 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7441 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7501 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7561 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7621 gttgaatact catattcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7681 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gtcagtgtta
     7741 caaccaatta accaattctg aacattatcg cgagcccatt tatacctgaa tatggctcat
     7801 aacacccctt gctcatgacc aaaatccctt aacgtgagtt acgcgcgcgt cgttccactg
     7861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8041 tgttcttcta gtgtagccgt agttagccca ccacttcaag aactctgtag caccgcctac
     8101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8521 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8581 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8641 cgagtcagtg agcgaggaag cggaaggcga gagtagggaa ctgccaggca tcaaactaag
     8701 cagaaggccc ctgacggatg gcctttttgc gtttctacaa actctttctg tgttgtaaaa
     8761 cgacggccag tcttaagctc gggccccctg ggcggttctg ataacgagta atcgttaatc
     8821 cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt tagggaaaga
     8881 gcatttgtca gaatatttaa gggcgcctgt cactttgctt gatatatgag aattatttaa
     8941 ccttataaat gagaaaaaag caacgcactt taaataagat acgttgcttt ttcgattgat
     9001 gaacacctat aattaaacta ttcatctatt atttatgatt ttttgtatat acaatatttc
     9061 tagtttgtta aagagaatta agaaaataaa tctcgaaaat aataaaggga aaatcagttt
     9121 ttgatatcaa aattatacat gtcaacgata atacaaaata taatacaaac tataagatgt
     9181 tatcagtatt tattatcatt tagaataaat tttgtgtcgc ccttaattgt gagcggataa
     9241 caattacgag cttcatgcac agtggcgttg actcaatatt ggccattagc catattattc
     9301 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat
     9361 cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgtt
//
