LOCUS       sequence-405795-        5739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing deltion and mutant TXNIP in retinal pigment 
            epithelium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Xue Y, Zhou Y, Cepko CL
  TITLE     Txnip deletions and missense alleles prolong the survival of cones 
            in a retinitis pigmentosa mouse model.
  JOURNAL   Elife. 2024 May 10;12:RP90749. doi: 10.7554/eLife.90749.
  PUBMED    38727583
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-05-10"; volume: "12:RP90749. doi"; pages: " 
            10.7554/eLife.90749"
COMMENT     Sequence Label: AAV-Best1-nt.Txnip(C247S)(1-320aa)
FEATURES             Location/Qualifiers
     source          1..5739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   259..388
                     /label=AAV2 ITR
     intron          1084..1216
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1199..1218
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(2250..2270)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2668..2679)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2746..2763)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2752..2959
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3047..3176
                     /label=AAV2 ITR
     primer_bind     complement(3195..3212)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3195..3211)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3204..3226)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3353..3808
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3440..3459)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3650..3671
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3834..3938
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3939..4007,4008..4799)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4157..4176)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4970..5558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5459..5478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagatcgga attcgccctt aagctagctc cttttcagat
      481 aagggcactg aggctgagag aggagctgaa acctacccgg ggtcaccaca cacaggtggc
      541 aaggctggga ccagaaacca ggactgttga ctgcagcccg gtattcattc tttccatagc
      601 ccacagggct gtcaaagacc ccagggccta gtcagaggct cctccttcct ggagagttcc
      661 tggcacagaa gttgaagctc agcacagccc cctaaccccc aactctctct gcaaggcctc
      721 aggggtcaga acactggtgg agcagatcct ttagcctctg gattttaggg ccatggtaga
      781 gggggtgttg ccctaaattc cagccctggt ctcagcccaa caccctccaa gaagaaatta
      841 gaggggccat ggccaggctg tgctagccgt tgcttctgag cagattacaa gaagggacta
      901 agacaaggac tcctttgtgg aggtcctggc ttagggagtc aagtgacggc ggctcagcac
      961 tcacgtgggc agtgccagcc tctaagagtg ggcaggggca ctggccacag agtcccaggg
     1021 agtcccacca gcctagtcgc cagacccaga tcctgcagaa gttggtcgtg aggcactggg
     1081 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
     1141 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
     1201 gcctttctct ccacaggtgt ccaggcatgg tgatgttcaa gaagatcaag tcttttgagg
     1261 tggtcttcaa cgaccccgag aaagtgtacg gcagcgggga gaaggtggcc ggacgggtaa
     1321 ttgtggaagt gtgtgaagtt acccgagtca aagctgtcag gatcctggct tgcggcgtgg
     1381 ccaaggtcct gtggatgcaa gggtctcagc agtgcaaaca gactttggac tacttgcgct
     1441 atgaagacac acttctccta gaagagcagc ctacagcagg tgagaacgag atggtgatca
     1501 tgaggcctgg aaacaaatat gagtacaagt tcggcttcga gcttcctcaa gggcccctgg
     1561 gaacatcctt taaaggaaaa tatggttgcg tagactactg ggtgaaggct tttctcgatc
     1621 gccccagcca gccaactcaa gaggcaaaga aaaacttcga agtgatggat ctagtggatg
     1681 tcaatacccc tgacttaatg gcaccagtgt ctgccaaaaa ggagaagaaa gtttcctgca
     1741 tgttcattcc tgatggacgt gtgtcagtct ctgctcgaat tgaccgaaaa ggattctgtg
     1801 aaggtgatga catctccatc catgctgact ttgagaacac gtgttcccga atcgtggtcc
     1861 ccaaagcggc tattgtggcc cgacacactt accttgccaa tggccagacc aaagtgttca
     1921 ctcagaagct gtcctcggtc agaggcaatc acattatctc agggactagc gcatcgtggc
     1981 gtggcaagag cctcagagtg cagaagatca gaccatccat cctgggctgc aacatcctca
     2041 aagtcgaata ctccttgctg atctacgtca gtgtccctgg ctccaagaaa gtcatccttg
     2101 atctgcccct agtgattggc agcaggtctg gtctgagcag ccggacatcc agcatggcca
     2161 gccggacgag ctctgagatg agctggtagt ggatccaatc aacctctgga ttacaaaatt
     2221 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2281 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2341 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     2401 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     2461 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     2521 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     2581 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     2641 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     2701 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgag atctgcctcg actgtgcctt
     2761 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     2821 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     2881 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     2941 atagcaggca tgctggggac tcgagttaag ggcgaattcc cgataaggat cttcctagag
     3001 catggctacg tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg
     3061 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     3121 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt
     3181 aattaaccta attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3241 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3301 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     3361 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3421 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3481 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3541 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3601 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3661 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3721 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3781 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     3841 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3901 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3961 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4021 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4081 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4141 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4201 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4261 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4321 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4381 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4441 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4501 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4561 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4621 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4681 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4741 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4801 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4861 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4921 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4981 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5041 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5101 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5161 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5221 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5281 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5341 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5401 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5461 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5521 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5581 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5641 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5701 cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
