LOCUS       sequence-405798-        5211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing deltion TXNIP in retinal pigment epithelium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Xue Y, Zhou Y, Cepko CL
  TITLE     Txnip deletions and missense alleles prolong the survival of cones 
            in a retinitis pigmentosa mouse model.
  JOURNAL   Elife. 2024 May 10;12:RP90749. doi: 10.7554/eLife.90749.
  PUBMED    38727583
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-05-10"; volume: "12:RP90749. doi"; pages: " 
            10.7554/eLife.90749"
COMMENT     Sequence Label: AAV-Best1-sC.Txnip
FEATURES             Location/Qualifiers
     source          1..5211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   259..388
                     /label=AAV2 ITR
     intron          1084..1216
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1199..1218
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(1722..1742)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2140..2151)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2218..2235)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2224..2431
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2519..2648
                     /label=AAV2 ITR
     primer_bind     complement(2667..2684)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2667..2683)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2676..2698)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2825..3280
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2912..2931)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3122..3143
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3306..3410
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3411..3479,3480..4271)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3629..3648)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4442..5030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4931..4950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagatcgga attcgccctt aagctagctc cttttcagat
      481 aagggcactg aggctgagag aggagctgaa acctacccgg ggtcaccaca cacaggtggc
      541 aaggctggga ccagaaacca ggactgttga ctgcagcccg gtattcattc tttccatagc
      601 ccacagggct gtcaaagacc ccagggccta gtcagaggct cctccttcct ggagagttcc
      661 tggcacagaa gttgaagctc agcacagccc cctaaccccc aactctctct gcaaggcctc
      721 aggggtcaga acactggtgg agcagatcct ttagcctctg gattttaggg ccatggtaga
      781 gggggtgttg ccctaaattc cagccctggt ctcagcccaa caccctccaa gaagaaatta
      841 gaggggccat ggccaggctg tgctagccgt tgcttctgag cagattacaa gaagggacta
      901 agacaaggac tcctttgtgg aggtcctggc ttagggagtc aagtgacggc ggctcagcac
      961 tcacgtgggc agtgccagcc tctaagagtg ggcaggggca ctggccacag agtcccaggg
     1021 agtcccacca gcctagtcgc cagacccaga tcctgcagaa gttggtcgtg aggcactggg
     1081 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
     1141 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
     1201 gcctttctct ccacaggtgt ccaggcatga gcctcagagt gcagaagatc agaccatcca
     1261 tcctgggctg caacatcctc aaagtcgaat actccttgct gatctacgtc agtgtccctg
     1321 gctccaagaa agtcatcctt gatctgcccc tagtgattgg cagcaggtct ggtctgagca
     1381 gccggacatc cagcatggcc agccggacga gctctgagat gagctggata gacctaaaca
     1441 tcccagatac cccagaagct cctccttgct atatggacat cattcctgaa gatcacagac
     1501 tagagagccc caccacccct ctgctggacg atgtggacga ctctcaagac agccctatct
     1561 ttatgtacgc ccctgagttc cagttcatgc ccccacccac ttacactgag gtggatccgt
     1621 gcgtccttaa caacaacaac aacaacaaca acgtgcagta gtggatccaa tcaacctctg
     1681 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     1741 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     1801 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     1861 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     1921 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     1981 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     2041 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     2101 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     2161 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg agatctgcct
     2221 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     2281 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     2341 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     2401 attgggaaga caatagcagg catgctgggg actcgagtta agggcgaatt cccgataagg
     2461 atcttcctag agcatggcta cgtagataag tagcatggcg ggttaatcat taactacaag
     2521 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     2581 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     2641 gcgcgcagcc ttaattaacc taattcactg gccgtcgttt tacaacgtcg tgactgggaa
     2701 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2761 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2821 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2881 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2941 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3001 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3061 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3121 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3181 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3241 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     3301 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3361 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3421 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3481 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3541 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3601 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3661 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3721 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3781 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3841 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3901 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3961 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4021 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4081 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4141 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4201 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4261 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4321 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4381 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4441 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4501 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4561 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4621 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4681 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4741 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4801 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4861 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4921 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4981 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5041 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5101 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5161 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa g
//
