LOCUS       sequence-405827-        9719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Weiss WA, Aldape K, Mohapatra G, Feuerstein BG, Bishop JM
  TITLE     Targeted expression of MYCN causes neuroblastoma in transgenic mice.
  JOURNAL   EMBO J. 1997 Jun 2;16(11):2985-95.
  PUBMED    9214616
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "1997-06-2"; volume: "16(11)"; pages: "2985-95"
COMMENT     Sequence Label: pTH-MYCN
FEATURES             Location/Qualifiers
     source          1..9719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(18..36)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(19..36)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(54..70)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(54..70)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(67..89)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    78..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(102..108,109..126,127..132))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    147..168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(285..302)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(456..1044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(536..555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1215..2006,2007..2075))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1838..1857
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2076..2180)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2258..2713)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2395..2416)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2607..2626
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     intron          7402..7974
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag
       61 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      121 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      181 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      241 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      301 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      361 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      421 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      481 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      541 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      601 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      661 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      721 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      781 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      841 gtaggcggtg ctacagagtt cttgacgtgg tggcctaact acggctacac tagaagaaca
      901 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      961 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1021 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1081 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1141 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1201 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1261 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1321 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1381 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1441 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1501 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1561 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1621 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1681 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1741 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1801 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1861 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1921 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1981 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2041 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2101 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2161 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgatgc ggtgtgaaat
     2221 accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg
     2281 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     2341 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     2401 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     2461 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     2521 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     2581 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     2641 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     2701 ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     2761 gggcctcttc gctattacgc cagctggcga aaggggatcg aattcggcct agagcctagg
     2821 ttgagacggg ggctgatcag cagctcttgc ctctggtctg actttcaact gcccaattat
     2881 ccctaagtgt ctcctatcga ccgaccgcag ctggtgctgt ccggtacgtg ttctgagcct
     2941 acagccccga ggctgctgct ccatccgatg gcctcgttag ggctaattgc tctggcattt
     3001 gggcctgatg aggacaagaa tggctggacc ctaccagatg tcagggagct tcattctctt
     3061 tccagtcaga ttggtgagct gtgtcaggga caaagggccc tgcggtctca ctgagcatca
     3121 ggtccctgct ttacaaccca tctcagtctc ctggtggcag ggaaaagcaa cctcactctc
     3181 ccttctgtct tgggctcacc ctgcacagca cttaggcaac tggtcatgat gtcttgacca
     3241 acttaaatcc aagagaagct cccactgcct ttcagggtat gtcagttgat gatttcatgg
     3301 acctgcatct ctgtgagcag gaacactgac cccagagccc atctggtgac aggattcttg
     3361 agaccctggc atcatgtgtt tctatagaaa gcgatttggg tggacttgtc aaagtgggtg
     3421 gggtatgtgt ttgcgtgtgt gtgtgtgggg gtctataggt atagcatgtt tgtgaatgca
     3481 tgaacatatt ataatacatg tatgcctatg cgattctgtt tgtatgtgta tatctgtgtt
     3541 cgcatatgtt tgtgtgtgca tgtggctgct cctatgtata cttgtgtatg tatttgtgtg
     3601 tctgtgtctc catgtataag cactgtgaat ctgtatatct gtatgtgcat acgtttgtgt
     3661 gtctatgtgt ctatgtatat accattgtgt atatacattt gtgtgtgcca atctctctct
     3721 gaatgtatct gtatatggcc atatgtgtac atgtatgtct ttatacatgt gtgtatgcct
     3781 atgtatgtcc tcatatatgt gtggtcatgt gtctctgtgt gtacatgctt ctgcgtgtct
     3841 atggtagaag gtcatgatgc tggttgaaag tggcctttga gcctaggttg tagaggcatg
     3901 tgttccaaaa tgcacatagg tttccatagt gcccaaagct atggggtaga tctggggtcc
     3961 ttgctccaag accaaggatc aggacacccc ctagtcctct gcctccttag ttccatgatg
     4021 tcctgggcag ggaggtccat atcagcaggg tgcatacagg atagcacagc agcctccaca
     4081 ccagcaacac tgatgccagg gagtgggtga gctgtcatta cggacaatta agcaccgtgc
     4141 tgagagagaa aggccccgtg tgctccccgt ctctgcaggc ctaatcgctg tggtgacaga
     4201 ggagggtaat tatcaggacg gctgtgaatg gcacaggcat tacaggggtg taaagggctc
     4261 aggaaggagg gagggcctgc tatgccccca gcacgcatct gagcccagag caggcaatgg
     4321 cccactgcac tggccttgcg cttcagatca gccccctttc ttaactgcta ggggatgctt
     4381 cccaatcact cctctaggct ctgcggcttg gcttccagcc tgtacgctgt ccagagagcc
     4441 ttcaaagcct cacttcgacc aaccagaagc ctctcgtcag ccctgccctg acctcgtgtg
     4501 cctcttcaaa gtgagattta gcagctgcag ctgggggtgc ctgagcccca ctcatgctgt
     4561 cttccttgaa gacagaagtg ttgggagctg aggacctggg ccgtatgatc cagagaagta
     4621 gtgtgcttct gggtctcagc tctcccttct gcagaatggg tctgtctgaa atggaaaggc
     4681 aggtgcccct ctgcagggcc taatctgagt cgccatgagt ggttaaaaga tccagcttgt
     4741 ctgtgggtga gctttgagag gaggcaggga cctctagcat ggaacagggc tgagtcctgg
     4801 aaagctgacc aagggcaggc ctaagaggcc tcttgggatt cttctcatca aaaagggcat
     4861 gggacacagc taaagcgtcc agggctcctc tgtgcccaca gatgccttag atcttggcac
     4921 aatgtagtca gccagctccg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     4981 tgtgtgtgtg tgtgtgtgtt tgcatgtatc tcacagacag tgcacaatgg cctggatgtg
     5041 aacagaggca agagtctggg ccagcagttg tctcccagga gggtccaaag acatcgtatt
     5101 ttcaagttta ggccaggtgc tcacttgggt gagctcagac acagacaaag gtctggagag
     5161 cacacattcc ccacccccac ccagcttcta tgcaagcacc tccagctgag acaagaaaac
     5221 gaattaaaaa gcaatatttg tgtcagcgta agacatttgc cgaaaggtta aatccacact
     5281 cgtggtgctg cacagcagcc ccctgtgcag gatttgttag gcacagctcc ctcctacccc
     5341 gtgccacctg agcaaatgcc aggctgggtg ggctggaacc agcctgggct tgcctcaccc
     5401 tggaatcccc agcaccctcc aaaggaggac cctgggagtg ggcatagacg cccttcaggt
     5461 gtgggcaaca gcccccagtc ctcaggatga aaggctaagg tgcagccagc tctgccttca
     5521 cggtgggaat gtctctatgt gagccctttc tgggctgtga agaacgctct gagaagggtc
     5581 ctgggaccct ggataggcca gagctgtgct gggcatgtag agacaggagt gggctaaagc
     5641 agcaaaggca ctgaccaagg aagagttcag agaggagcgt ggaatatggg gaggggttca
     5701 tagtaagaga gagcaggcag tggagagtaa atagtcactg agccggggtt tatggggttt
     5761 gtaggagctt actcagagaa agtagatgag agatgccatg ccagtctgag tatcacagag
     5821 ccccaggctc tcctgggaac ggaactgtga gggccagaag gtcagcaagg gaggttaggg
     5881 agagttcctt ttgtactgac tcagcattta tcctgctccc agggggcaat gggggccagt
     5941 gagggatgca gagcaaggca gtgatgtggc aggcagttcc tgttgtgaaa gagctgggaa
     6001 gggagcgggc tgggcctggt acgtacagca ggccatttct gagggtccga gtgctgtcta
     6061 ggaggtgcag tgagacttca gtgatcagcc agaacagaag ctaagcgggg tggggactgc
     6121 gagttcaggc ttctgggtct tgcaaatatc cagaatgcta aatcctcaga accccagggt
     6181 ggccattttc agagtgggtt ttgtcctttg ggcacttgtg cagactccaa tatccagagg
     6241 gataaggatg gtactcttca gtacccttag tgagaggaca cttttctctg aagggcttga
     6301 atgtgccgag ccattacctg aaggaaggaa atgactccag ggacatagga tgggcccagc
     6361 acaactcacc tgctacagag aaaggtcccc tccctggtct ccttagagat cctgtttccc
     6421 tggctgagga agctagggtg gatctttgtg taagtgggtg tggatgctaa ctggaaaaca
     6481 aaaggtcact tactgttaga cctcggggta ccatggaaga gatgatcact gagtgtgccc
     6541 ttacatgggg accagctgag aatggggcta ccactagctc gagaccatga tacagggaat
     6601 aagtgtgcat ttgggggtag ggagtggctc agaatactct taaccaaagc agaggtttgc
     6661 tcccacagga aggtgaggtc agaaggcctt agggagctgc caggggctag ggttggcacc
     6721 atctcccagg ctgtgtcttt aaggagatga taatcagagg gatagaacct tgcaaaagtg
     6781 ggccagtctt gggaatacta tagaggaata gccttctgga acattctgtg tctcatagga
     6841 cctgcctggg gatccagccc cagtgccagc acatataccg actggggcag tgaatagata
     6901 gtacactttg ttacatgggc tggggggaac atggcccatg tcctggaggg gactttatga
     6961 cagacatcca aaaatccagt gagagggctt ctagatttgt ctccaaaggt tatagttcta
     7021 acatgagccc ttaggaaatc cagcatggtt ctccctgtgt gccctggttt ggttagagag
     7081 ctctagcggt ctcctgtccc acagaatacc agccagcccc tgccctacgt cgtgcctcgg
     7141 gctgagggtg attcagaggc aggtgcctgt gacagtggat gcaattagat ctaatgggac
     7201 ggaggccttt ctcgtcgccc tcgctccatg cccacccccg cctccctcag gcacagcagg
     7261 cgtggagagg atgcgcagga ggtaggaggt gggggaccca gaggggcttt gacgtcagcc
     7321 tggcctttaa agagggcgcc tgcctggcga gggctgtgga gacccccggg tcgcgagtcg
     7381 accgatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct
     7441 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga
     7501 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc
     7561 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac
     7621 tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag
     7681 tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca
     7741 cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat
     7801 tctggctggc gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg
     7861 cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag
     7921 tccaaaccgg gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg
     7981 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattccg actctagaag
     8041 atctgtctgt gtttgagctg tcggagagcc gtgttggagg tcggcgccgg cccccgcctt
     8101 ccgcgccccc cacgggaagg aagcaccccc ggtattaaaa cgaacggggc ggaaagaagc
     8161 cctcagtcgc cggccgggag gcgagccgat gccgagctgc tccacgtcca ccatgccggg
     8221 catgatctgc aagaacccag acctcgagtt tgactcgcta cagccctgct tctacccgga
     8281 cgaagatgac ttctacttcg gcggccccga ctcgaccccc ccgggggagg acatctggaa
     8341 gaagtttgag ctgctgccca cgcccccgct gtcgcccagc cgtggcttcg cggagcacag
     8401 ctccgagccc ccgagctggg tcacggagat gctgcttgag aacgagctgt ggggcagccc
     8461 ggccgaggag gacgcgttcg gcctgggggg actgggtggc ctcaccccca acccggtcat
     8521 cctccaggac tgcatgtgga gcggcttctc cgcccgcgag aagctggagc gcgccgtgag
     8581 cgagaagctg cagcacggcc gcgggccgcc aaccgccggt tccaccgccc agtccccggg
     8641 agccggcgcc gccagccctg cgggtcgcgg gcacggcggg gctgcgggag ccggccgcgc
     8701 cggggccgcc ctgcccgccg agctcgccca cccggccgcc gagtgcgtgg atcccgccgt
     8761 ggtcttcccc tttcccgtga acaagcgcga gccagcgccc gtgcccgcag ccccggccag
     8821 tgccccggcg gcgggccctg cggtcgcctc gggggcgggt attgccgccc cagccggggc
     8881 cccgggggtc gcccctccgc gcccaggcgg ccgccagacc agcggcggcg accacaaggc
     8941 cctcagtacc tccggagagg acaccctgag cgattcagat gatgaagatg atgaagagga
     9001 agatgaagag gaagaaatcg acgtggtcac tgtggagaag cggcgttcct cctccaacac
     9061 caaggctgtc accacattca ccatcactgt gcgtcccaag aacgcagccc tgggtcccgg
     9121 gagggctcag tccagcgagc tgatcctcaa acgatgcctt cccatccacc agcagcacaa
     9181 ctatgccgcc ccctctccct acgtggagag tgaggatgca cccccacaga agaagataaa
     9241 gagcgaggcg tccccacgtc cgctcaagag tgtcatcccc ccaaaggcta agagcttgag
     9301 cccccgaaac tctgactcgg aggacagtga gcgtcgcaga aaccacaaca tcctggagcg
     9361 ccagcgccgc aacgaccttc ggtccagctt tctcacgctc agggaccacg tgccggagtt
     9421 ggtaaagaat gagaaggccg ccaaggtggt cattttgaaa aaggccactg agtatgtcca
     9481 ctccctccag gccgaggagc accagctttt gctggaaaag gaaaaattgc aggcaagaca
     9541 gcagcagttg ctaaagaaaa ttgaacacgc tcggacttgc tagacgcttc tcaaaactgg
     9601 acagtcactg ccactttgca cattttgatt ttttttttaa acggaattcc tcgacggatc
     9661 cgtgacaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cgcggccgc
//
