LOCUS       sequence-405835-        9690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For marking CRISPR HDR integrant with DsRed expressed in flight 
            muscles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT10
FEATURES             Location/Qualifiers
     source          1..9690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             127..918
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1164..1752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1653..1672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1906..1923
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1955..1973
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1955..1972
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        2050..2470
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    2477..2600
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2623..2628,2629..2646,2647..2653)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        2696..2800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2801..2869,2870..3661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3019..3038)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4182..4201
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4341..4464)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    4533..4667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4557..4576)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4611..4630
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(4775..5449,5450..5452,5453..5455))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(4837..4860)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5035..5054)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5252..5272
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     regulatory      5452..5461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6326..6449
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6472..6477,6478..6495,6496..6502)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6556..7215
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6622..6641)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        7224..7669
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(7670..7756,7757..9091)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(9130..9253)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9345..9364)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9346..9364)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
       61 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      121 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
      181 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
      241 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
      301 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
      361 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
      421 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
      481 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
      541 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
      601 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
      661 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
      721 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
      781 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
      841 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
      901 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc
      961 gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     1021 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     1081 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1141 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1201 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1261 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     1321 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1381 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1441 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1501 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1561 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1621 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1681 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1741 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1801 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1861 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1921 gaggaaatcg tcacctgcag gaaggtttaa acgcatttag gtgacactat agaagtgtgt
     1981 atcgctcgag ggatccgaat tcgaagtctt ggtacggagg ggatccgatc gacaagccga
     2041 acatatgggt tttttgctca cctgtgattg ctcctactca aatacaaaaa catcaaattt
     2101 tctgtcaata aagcatattt atttatattt attttacagg aaagaattcc ttttaaagtg
     2161 tattttaacc tataatgaaa aacgattaaa aaaaatacat aaaataattc gaaaattttt
     2221 gaatagccca ggttgataaa aattcatttc atacgtttta taacttatgc ccctaagtat
     2281 tttttgacca tagtgtttca attctacatt aattttacag agtagaatga aacgccacct
     2341 actcagccaa gaggcgaaaa ggttagctcg ccaagcagag agggcgccag tgctcactac
     2401 tttttataat tctcaacttc tttttccaga ctcagttcgt atatatagac ctattttcaa
     2461 tttaacgtcg gggcctcaac tttgtataat aaagttgaac gagaaacgta aaatgatata
     2521 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
     2581 caacatatcc agtcactatg ggccgcatta ggcaccccag gctttacact ttatgcttcc
     2641 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctacgcgg
     2701 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2761 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2821 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2881 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2941 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3001 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3061 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3121 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3181 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3241 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3301 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3361 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3421 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3481 gtttattgct gataaatctg gagccggtga gcgtgggtcc cgcggtatca ttgcagcact
     3541 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3601 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3661 atggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     3721 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa
     3781 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     3841 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     3901 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     3961 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta
     4021 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta tcgatacacc
     4081 gggccgtcgc atggttattc cacttgcgtc tgcgcgcttg ctctcagata aagtctcccg
     4141 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     4201 ggccagtgtg ccggtttccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     4261 tgacatcaaa aacgccatta acctgatgtt ctggggaata taatgtcagg ctcccttata
     4321 cacagccagt ctgcacgtcg catagtgact ggatatgttg tgttttacag tattatgtag
     4381 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     4441 gttcaacttt tctatacaaa gttgtccagc tccgagctcg gtacctcgcg aatgcatcta
     4501 gatgcaccat tatgttccgg aggcgcgcca tcgatccaga catgataaga tacattgatg
     4561 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     4621 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     4681 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     4741 acctctacaa atgtggtatg gctgattatg atctctacag gaacaggtgg tggcggccct
     4801 cggtgcgctc gtactgctcc acgatggtgt agtcctcgtt gtgggaggtg atgtccagct
     4861 tggagtccac gtagtagtag ccgggcagct gcacgggctt cttggccatg tagatggact
     4921 tgaactccac caggtagtgg ccgccgtcct tcagcttcag ggccttgtgg atctcgccct
     4981 tcagcacgcc gtcgcggggg tacaggcgct cggtggaggc ctcccagccc atggtcttct
     5041 tctgcattac ggggccgtcg gaggggaagt tcacgccgat gaacttcacc ttgtagatga
     5101 agcagccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg tcctcgaagt
     5161 tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcttgtag tcggggatgt
     5221 cggcggggtg cttcacgtac accttggagc cgtactggaa ctggggggac aggatgtccc
     5281 aggcgaaggg cagggggccg cccttggtca ccttcagctt cacggtgttg tggccctcgt
     5341 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gtgccctcca
     5401 tgcgcacctt gaagcgcatg aactccttga tgacgttctt ggaggagcgc accatggtgg
     5461 cgaccggtgg atcccgggcc cgcggtaccg tcgactctag cggtaccccg attgtttagc
     5521 ttgttcagct gcgcttgttt atttgcttag ctttcgctta gcgacgtgtt cactttgctt
     5581 gtttgaattg aattgtcgct ccgtagacga agcgcctcta tttatactcc ggcggtcgag
     5641 ggttcctaaa tatagatcac ggatccgata cgaatatgta tatgtatacc acttatatat
     5701 atatatatca gaatgctttg catatatacg gattttcggg gcgttggtat ttgttacgta
     5761 acgccaattt tcatttagat atttgtgtac atttctccag gtttctttgt gaaaaaatct
     5821 acagactatt gatacgccaa aacaatgctt attattacaa atgtagagag gttaaactgt
     5881 caccacaact cactcaactg gtaactacta ccgtttggaa cgcgatttcg atattttgaa
     5941 caggtatctc gatatggtgg cgtgttctca tacaagcaat tcacgagatc gcaatgattc
     6001 ctttcagtaa acttcgaagg cggaacgatt ttcgacaatc cacactaaat cgacaatcga
     6061 aaattcgatt cgaattgacg cgaactttta acgaatgcgt tatttttaaa attctcgaaa
     6121 ttatttcgaa actaaagtca catacatatt cgtctatatg taattcgtaa tttcgagaag
     6181 ctttttggct catgctacca catggcacac atagacataa tgtataagta ttggcgatct
     6241 ctttctaatg tctttggtta tttttaaaac atattcgaaa ttatttcgcc catgtatatg
     6301 agccgacaac gaattcgagt ataatcaact ttgtatacaa aagttgaacg agaaacgtaa
     6361 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     6421 tgtaaaacac aacatatcca gtcactatgg gccgcattag gcaccccagg ctttacactt
     6481 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
     6541 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     6601 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     6661 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     6721 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     6781 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     6841 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     6901 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     6961 aaagggttta ttgagaatat gtttttcgtg tcagccaatc cctgggtgag tttcaccagt
     7021 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     7081 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     7141 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     7201 cagggcgggg cgtaaacgcg tggcacatat acctgccgtt cactattatt tagtgaaatg
     7261 agatattatg atattttctg aattgtgatt aaaaaggcaa ctttatgccc atgcaacaga
     7321 aactataaaa aatacagaga atgaaaagaa acagatagat tttttagttc tttaggcccg
     7381 tagtctgcaa atccttttat gattttctat caaacaaaag aggaaaatag accagttgca
     7441 atccaaacga gagtctaata gaatgaggtc gaaaagtaaa tcgcgcgggt ttgttactga
     7501 taaagcaggc aagacctaaa atgtgtaaag ggcaaagtgt atactttggc gtcacccctt
     7561 acatatttta ggtctttttt tattgtgcgt aactaacttg ccatcttcaa acaggagggc
     7621 tggaagaagc agaccgctaa cacagtacat aaaaaaggag acatgaacga tgaacatcaa
     7681 aaagtttgca aaacaagcaa cagtattaac ctttactacc gcactgctgg caggaggcgc
     7741 aactcaagcg tttgcgaaag aaacgaacca aaagccatat aaggaaacat acggcatttc
     7801 ccatattaca cgccatgata tgctgcaaat ccctgaacag caaaaaaatg aaaaatatca
     7861 agttcctgaa ttcgattcgt ccacaattaa aaatatctct tctgcaaaag gcctggacgt
     7921 ttgggacagc tggccattac aaaacgctga cggcactgtc gcaaactatc acggctacca
     7981 catcgtcttt gcattagccg gagatcctaa aaatgcggat gacacatcga tttacatgtt
     8041 ctatcaaaaa gtcggcgaaa cttctattga cagctggaaa aacgctggcc gcgtctttaa
     8101 agacagcgac aaattcgatg caaatgattc tatcctaaaa gaccaaacac aagaatggtc
     8161 aggttcagcc acatttacat ctgacggaaa aatccgttta ttctacactg atttctccgg
     8221 taaacattac ggcaaacaaa cactgacaac tgcacaagtt aacgtatcag catcagacag
     8281 ctctttgaac atcaacggtg tagaggatta taaatcaatc tttgacggtg acggaaaaac
     8341 gtatcaaaat gtacagcagt tcatcgatga aggcaactac agctcaggcg acaaccatac
     8401 gctgagagat cctcactacg tagaagataa aggccacaaa tacttagtat ttgaagcaaa
     8461 cactggaact gaagatggct accaaggcga agaatcttta tttaacaaag catactatgg
     8521 caaaagcaca tcattcttcc gtcaagaaag tcaaaaactt ctgcaaagcg ataaaaaacg
     8581 cacggctgag ttagcaaacg gcgctctcgg tatgattgag ctaaacgatg attacacact
     8641 gaaaaaagtg atgaaaccgc tgattgcatc taacacagta acagatgaaa ttgaacgcgc
     8701 gaacgtcttt aaaatgaacg gcaaatggta cctgttcact gactcccgcg gatcaaaaat
     8761 gacgattgac ggcattacgt ctaacgatat ttacatgctt ggttatgttt ctaattcttt
     8821 aactggccca tacaagccgc tgaacaaaac tggccttgtg ttaaaaatgg atcttgatcc
     8881 taacgatgta acctttactt actcacactt cgctgtacct caagcgaaag gaaacaatgt
     8941 cgtgattaca agctatatga caaacagagg attctacgca gacaaacaat caacgtttgc
     9001 gccaagcttc ctgctgaaca tcaaaggcaa gaaaacatct gttgtcaaag acagcatcct
     9061 tgaacaagga caattaacag ttaacaaata aatgtcaggc tcccttatac acagccagtc
     9121 tgcacgtcgc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     9181 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcaactttt
     9241 taatacaaag ttgcctccgg aacataatgg tgcatctaga tgcccattcc tgtagacttc
     9301 ttaattaaga cgtcagaatt ctcgaggcgg ccgcatgtga gtctccctat agtgagtcgt
     9361 attaatttcg cgggcgcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt
     9421 taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc
     9481 gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg
     9541 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     9601 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     9661 gttttcatgg cttgttatga ctgttttttt
//
