LOCUS       sequence-405841-       10941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For inserting a phiC31 attP site. HDR integrant is marked with 
            ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 
            promoter allows use of two different gRNAs in two synthesized arms..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 10941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT31
FEATURES             Location/Qualifiers
     source          1..10941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(445..531,532..1866)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(1905..2028)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2081..2877)
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     primer_bind     complement(2926..2945)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2927..2945)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3398..4189
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4435..5023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4924..4943
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5177..5194
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        5226..5244
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5226..5243
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        5321..5741
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    5748..5871
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5894..5899,5900..5917,5918..5924)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        5967..6071
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6072..6140,6141..6932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6290..6309)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     7453..7472
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7612..7735)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    7808..7907
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     promoter        8236..8827
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     primer_bind     complement(9199..9219)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     polyA_signal    9804..9938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9841..9860)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9895..9914
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    10042..10165
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(10188..10193,10194..10211,10212..10218)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             10272..10931
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10338..10357)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        join(10940..10941,1..444)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 catatacctg ccgttcacta ttatttagtg aaatgagata ttatgatatt ttctgaattg
       61 tgattaaaaa ggcaacttta tgcccatgca acagaaacta taaaaaatac agagaatgaa
      121 aagaaacaga tagatttttt agttctttag gcccgtagtc tgcaaatcct tttatgattt
      181 tctatcaaac aaaagaggaa aatagaccag ttgcaatcca aacgagagtc taatagaatg
      241 aggtcgaaaa gtaaatcgcg cgggtttgtt actgataaag caggcaagac ctaaaatgtg
      301 taaagggcaa agtgtatact ttggcgtcac cccttacata ttttaggtct ttttttattg
      361 tgcgtaacta acttgccatc ttcaaacagg agggctggaa gaagcagacc gctaacacag
      421 tacataaaaa aggagacatg aacgatgaac atcaaaaagt ttgcaaaaca agcaacagta
      481 ttaaccttta ctaccgcact gctggcagga ggcgcaactc aagcgtttgc gaaagaaacg
      541 aaccaaaagc catataagga aacatacggc atttcccata ttacacgcca tgatatgctg
      601 caaatccctg aacagcaaaa aaatgaaaaa tatcaagttc ctgaattcga ttcgtccaca
      661 attaaaaata tctcttctgc aaaaggcctg gacgtttggg acagctggcc attacaaaac
      721 gctgacggca ctgtcgcaaa ctatcacggc taccacatcg tctttgcatt agccggagat
      781 cctaaaaatg cggatgacac atcgatttac atgttctatc aaaaagtcgg cgaaacttct
      841 attgacagct ggaaaaacgc tggccgcgtc tttaaagaca gcgacaaatt cgatgcaaat
      901 gattctatcc taaaagacca aacacaagaa tggtcaggtt cagccacatt tacatctgac
      961 ggaaaaatcc gtttattcta cactgatttc tccggtaaac attacggcaa acaaacactg
     1021 acaactgcac aagttaacgt atcagcatca gacagctctt tgaacatcaa cggtgtagag
     1081 gattataaat caatctttga cggtgacgga aaaacgtatc aaaatgtaca gcagttcatc
     1141 gatgaaggca actacagctc aggcgacaac catacgctga gagatcctca ctacgtagaa
     1201 gataaaggcc acaaatactt agtatttgaa gcaaacactg gaactgaaga tggctaccaa
     1261 ggcgaagaat ctttatttaa caaagcatac tatggcaaaa gcacatcatt cttccgtcaa
     1321 gaaagtcaaa aacttctgca aagcgataaa aaacgcacgg ctgagttagc aaacggcgct
     1381 ctcggtatga ttgagctaaa cgatgattac acactgaaaa aagtgatgaa accgctgatt
     1441 gcatctaaca cagtaacaga tgaaattgaa cgcgcgaacg tctttaaaat gaacggcaaa
     1501 tggtacctgt tcactgactc ccgcggatca aaaatgacga ttgacggcat tacgtctaac
     1561 gatatttaca tgcttggtta tgtttctaat tctttaactg gcccatacaa gccgctgaac
     1621 aaaactggcc ttgtgttaaa aatggatctt gatcctaacg atgtaacctt tacttactca
     1681 cacttcgctg tacctcaagc gaaaggaaac aatgtcgtga ttacaagcta tatgacaaac
     1741 agaggattct acgcagacaa acaatcaacg tttgcgccaa gcttcctgct gaacatcaaa
     1801 ggcaagaaaa catctgttgt caaagacagc atccttgaac aaggacaatt aacagttaac
     1861 aaataaatgt caggctccct tatacacagc cagtctgcac gtcgcatagt gactggatat
     1921 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     1981 tatttatatc attttacgtt tctcgttcaa ctttttaata caaagttgcc tccggaacat
     2041 aatggtgcat ctagatgccc attcctgtag acttcttaat cgaagttcac ccggatatct
     2101 ttcctatata tactgtactc ttgcagaata tgtacttggg aatcataagg atgcgaagga
     2161 cgtacgcgct gtgtctgtaa ctaaggggtt gctttcgggt acctaccatg ccaatgatgg
     2221 gactaccgcc ggataggaat cccttttggg tgcacaccgt cacctccagt tccgtctgcc
     2281 tgagttcggc aatggaaatc aggtactcaa aggatgcatc gtagacgggg ttgcagttgt
     2341 ccttgatcac gctcgtcttg cgtttcgact ccttggtgcg tccaggcaac agatacagct
     2401 taacatacgg atcggggata ttgctgggat cgcgaagtgg tatcttctgg attttgtgta
     2461 tggtcacgtc tagtttttga cgctgggcgc tgtagcggat cgacaactgc attcgtccaa
     2521 ggccatgttc gccgggtgag gaggtggagt ctggaaagcg gtgggtcagc acagaggtgg
     2581 ccagctcgct gatgggtttt tcgctggagg tggaggccga catggccggg gagattttcg
     2641 tagccgccac aggatcttct tcactgatgg gcgattcggc ggacaaacgc ttgctggatg
     2701 cctgagattc ctaaaagcaa tggcaaatta gattcaatta aaacagaaat tgtttatata
     2761 acagagtaaa agcaaaaaaa aaggaatcac agattctccc cgacggggaa ttgaaccccg
     2821 ttctcccgcg tgacaggcgg ggatactaac cactatacta tcgaggacgt tgaaaatgaa
     2881 ttcaattaag acgtcagaat tctcgaggcg gccgcatgtg agtctcccta tagtgagtcg
     2941 tattaatttc gcgggcgcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
     3001 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
     3061 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg
     3121 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     3181 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     3241 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     3301 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     3361 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
     3421 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     3481 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     3541 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     3601 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     3661 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     3721 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     3781 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     3841 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     3901 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     3961 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     4021 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     4081 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     4141 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     4201 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     4261 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     4321 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     4381 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4441 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4501 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4561 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4621 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4681 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4741 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4801 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4861 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4921 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4981 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5041 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5101 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5161 ccgaacgacc gagcgcagcg agtcagtgag cgaggaaatc gtcacctgca ggaaggttta
     5221 aacgcattta ggtgacacta tagaagtgtg tatcgctcga gggatccgaa ttcgaagtct
     5281 tggtacggag gggatccgat cgacaagccg aacatatggg ttttttgctc acctgtgatt
     5341 gctcctactc aaatacaaaa acatcaaatt ttctgtcaat aaagcatatt tatttatatt
     5401 tattttacag gaaagaattc cttttaaagt gtattttaac ctataatgaa aaacgattaa
     5461 aaaaaataca taaaataatt cgaaaatttt tgaatagccc aggttgataa aaattcattt
     5521 catacgtttt ataacttatg cccctaagta ttttttgacc atagtgtttc aattctacat
     5581 taattttaca gagtagaatg aaacgccacc tactcagcca agaggcgaaa aggttagctc
     5641 gccaagcaga gagggcgcca gtgctcacta ctttttataa ttctcaactt ctttttccag
     5701 actcagttcg tatatataga cctattttca atttaacgtc ggggcctcaa ctttgtataa
     5761 taaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
     5821 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat gggccgcatt
     5881 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
     5941 ggatccgtcg agattttcag gagctacgcg gaacccctat ttgtttattt ttctaaatac
     6001 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6061 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6121 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6181 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6241 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6301 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6361 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6421 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6481 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6541 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6601 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6661 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6721 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6781 agcgtgggtc ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6841 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6901 agataggtgc ctcactgatt aagcattggt aatggatccg gcttactaaa agccagataa
     6961 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     7021 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     7081 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     7141 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     7201 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     7261 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     7321 tggatgtaca gagtgatatt atcgatacac cgggccgtcg catggttatt ccacttgcgt
     7381 ctgcgcgctt gctctcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     7441 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtttcc gttatcgggg
     7501 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     7561 tctggggaat ataatgtcag gctcccttat acacagccag tctgcacgtc gcatagtgac
     7621 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     7681 atattgatat ttatatcatt ttacgtttct cgttcaactt ttctatacaa agttgtccag
     7741 ctgtactgac ggacacaccg aagccccggc ggcaaccctc agcggatgcc ccggggcttc
     7801 acgttttccc aggtcagaag cggttttcgg gagtagtgcc ccaactgggg taacctttga
     7861 gttctctcag ttgggggcgt agggtcgccg acatgacaca aggggttgtg accggggtgg
     7921 acacgtacgc gggtgcttac gaccgtcagt cgcgcgagcg cgaggttgct taagaataag
     7981 taattcttaa gcaaccatta cgccaagctt gcatgccgag ctcttacgcg cccgcgtaaa
     8041 acacaatcaa gtatgagtca taagctgatg tcatgttttg cacacggctc ataaccgaac
     8101 tggctttacg agtagaattc tacttgtaac gcacgatcag tggatgatgt catttgtttt
     8161 tcaaatcgag atgatgtcat gttttgcaca tggctcataa ctaaactcgc tttacgggta
     8221 gaattctacg cgcgtcgatg tctttgtgat gcgcgcgaca tttttgtagg ttattgataa
     8281 aatgaacgga tacgttgccc gacattatca ttaaatcctt ggcgtagaat ttgtcgggtc
     8341 cattgtccgt gtgcgctagc atgcccgtaa cggacctcgt acttttggct tcaaaggttt
     8401 tgcgcacaga caaaatgtgc cacacttgca gctctgcatg tgtgcgcgtt accacaaatc
     8461 ccaacggcgc agtgtacttg ttgtatgcaa ataaatctcg ataaaggcgc ggcgcgcgaa
     8521 tgcagctgat cacgtacgct cctcgtgttc cgttcaagga cggtgttatc gacctcagat
     8581 taatgtttat cggccgactg ttttcgtatc cgctcaccaa acgcgttttt gcattaacat
     8641 tgtatgtcgg cggatgttct atatctaatt tgaataaata aacgataacc gcgttggttt
     8701 tagagggcat aataaaagaa atattgttat cgtgttcgcc attagggcag tataaattga
     8761 cgttcatgtt ggatattgtt tcagttgcaa gttgacactg gcggcgacaa gatcgtgaac
     8821 aaccaagtga ccattaaccc tcgaccgccg gagtataaat agaggcgctt cgtctacgga
     8881 gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata
     8941 aacaagcgca gctgaacaag ctaaacaatc ggggtaccgc tagagtcgac ggtacgatcc
     9001 accggtcgcc accccatggt gcgctcctcc aagaacgtca tcaaggagtt catgcgcttc
     9061 aaggtgcgca tggagggcac cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     9121 ggccgcccct acgagggcca caacaccgtg aagctgaagg tgaccaaggg cggccccctg
     9181 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt gtacgtgaag
     9241 caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag
     9301 cgcgtgatga atttcgaaga tggtggagtt gtcacagtca cacaagattc ttcgctccaa
     9361 gatggttgtt ttatttacaa agtgaaattt attggagtca attttccgtc ggatggaccg
     9421 gttatgcaaa agaaaacaat gggttgggaa gcatcgacgg aacgtctgta tccacgagat
     9481 ggagttttga aaggcgaaat tcacaaagct cttaaactca aagatggcgg acattacctt
     9541 gtagaattta aaagcattta tatggctaag aaaccggtcc aactccctgg atattattat
     9601 gtcgattcga aactggatat tacttcacat aacgaagatt atacgatagt tgagcaatat
     9661 gaacgcactg aaggtcgtca ccatctcttt ctctaaagat cataatcagc cataccacat
     9721 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     9781 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     9841 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9901 tgtccaaact catcaatgta tcttatcatg tctggatcga tggcgcgcct ccggaacata
     9961 atggtgcatc tagatgcatt cgcgaggtac cgagctcgaa ttcactgggg ttgcttaaga
    10021 ataagtaatt cttaagcaac ccaactttgt atacaaaagt tgaacgagaa acgtaaaatg
    10081 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
    10141 aaacacaaca tatccagtca ctatgggccg cattaggcac cccaggcttt acactttatg
    10201 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
    10261 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
    10321 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
    10381 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
    10441 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
    10501 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
    10561 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
    10621 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
    10681 ggtttattga gaatatgttt ttcgtgtcag ccaatccctg ggtgagtttc accagttttg
    10741 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
    10801 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
    10861 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
    10921 gcggggcgta aacgcgtggc a
//
