LOCUS       sequence-405842-       13716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses RGG-BcLOV-EGFR for optogenetic activation of EGFR 
            signaling. Includes an mCherry tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13716)
  AUTHORS   Pal AA, Benman W, Mumford TR, Huang Z, Chow BY, Bugaj LJ
  TITLE     Optogenetic clustering and membrane translocation of the BcLOV4 
            photoreceptor.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Aug 8;120(32):e2221615120. doi: 
            10.1073/pnas.2221615120. Epub 2023 Aug 1.
  PUBMED    37527339
REFERENCE   2  (bases 1 to 13716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-08-8"; volume: "120(32):e2221615120. 
            doi"; pages: " 10.1073/pnas.2221615120. Epub 2023 Aug 1"
COMMENT     Sequence Label: RGG-BcLOV-mCh-EGFR
FEATURES             Location/Qualifiers
     source          1..13716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    231..348
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        438..741
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        742..945
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     896..916
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      983..992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3323..4030
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3468..3486)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3736..3755
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5813..6401
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5866..5886)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6284..6295)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6464..6697
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6722..6740)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6723..6740)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7030..7049)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     7383..7405
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7443..7461)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7529..7633
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7634..7702,7703..8494)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8665..9253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9154..9173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9407..9424
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9494..9514)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9499..9828
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9679..9814
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10962..11027
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11157..11177
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11602..11736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11639..11658)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11693..11712
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11905..12538
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12585..12710
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13202..13435
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13620..13664
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
       61 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
      121 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
      181 gattagtgaa cggatctcga cggtcgccaa atggcagtat tcatccacaa ttttaaaaga
      241 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
      301 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
      361 agggacagca gagatccagt ttggatcgat aagcttgata tcgaattctt agttcatagc
      421 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      481 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      541 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      601 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      661 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      721 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      781 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      841 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      901 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
      961 cagatccgct agcgctacgc gtgccaccat ggaatcgaat caatctaaca acggtggatc
     1021 gggaaacgca gcattgaatc gtgggggccg ttacgtacca ccacacttga gaggcggaga
     1081 tggtggtgcc gccgctgctg cctcagccgg aggcgacgac agacgcggtg gggccggggg
     1141 tggtggctat cgtagaggtg gtggaaattc agggggcggc ggcggaggag gctacgatcg
     1201 tggatacaac gacaaccggg atgaccgtga caatcggggc ggctccggcg gttatggccg
     1261 ggacagaaat tacgaggatc gcggttataa cgggggtggt gggggaggag gcaatcgcgg
     1321 ctacaataat aaccgcggtg ggggtggagg cggctataat cggcaggacc gtggagacgg
     1381 cggttctagt aatttctcgc ggggagggta taacaacaga gatgagggct cagacaacag
     1441 aggctcaggc agatcataca acaacgatcg gcgcgacaat ggtggtgacg ggggtagcgg
     1501 cagcggtagc atggccacag acgcaatcga ccattactac cagaacagag acggcccccc
     1561 ttccaggagc tttagcgacg aatacgactc atacaataag acagcccctc tgccacctct
     1621 gagcgcctgc ccagagcgcg cccagacccc taacagcaag agaatcccca tcacacccaa
     1681 ttccgccaag agcacctccg agtctcctcg gagaatcaca ttctctcgcg acgcaggcag
     1741 ctccaccaag ggcagcaccc agacaagccc atccaaccag ggctccgtgt ctagccggaa
     1801 tggctctaac ggaaatggcc tgggcctgtc ctctgccggc aacttcgccg atttctttag
     1861 ctccgaggtg ttttatatcg tgctgcacaa tcccaccaca gcccacagat tcctgaagtt
     1921 ttgccagagc agggcctgtt ccgagaacct ggagtttctg cagaagatcg acgagtacaa
     1981 taggctgatc gatgaggcca cacgcctgct gtctaccatc tacagcacat acgtgagcag
     2041 cgaggcccct aagcagatca acatctctaa tagccacgcc aggcgcctga gccacgacat
     2101 caagggagca acccacaacg tgctgcccgg cctggaggat atcttcaatg gctcccagga
     2161 gcagatcgag aagctgctgg cctctgacct gtatcctaga tttgtgaagc accaggtgac
     2221 cgcatccgcc acaatggccc tggcaaacga caaggagagg tacccaggcc tgggcgattg
     2281 cttctgtctg acagacccac ggagagccga taatcccatc gtgttcgcaa gcgacggatt
     2341 cgtgagcgtg accggctatt cccgctctga tatcatccca aggaactgcc gcttcctgca
     2401 gggcagcttt accgaccggc aggccacaaa gaggctgcgc acctccatcg agaatcgcga
     2461 ggagacagtg gagctgctgc tgaactatcg gaagaatggc gatccctttt ggaacctgct
     2521 gtacgtgtcc cctctgctgg acggcaatgg cgatgtgaga ttctttctgg gcggccagat
     2581 caactgttct accacaatcc acagccggac cgacgtgctg agaatcctga gcctgaacga
     2641 cgaggataca ggctccttca tcgagggctc taataagacc cccagcgtgc ggtccaagga
     2701 gtctaacaat gcctccaact atggcaagac atccttcttt aagtctttta agaagtacaa
     2761 ggccaatacc gtggagatca gagacgaggc cggcatggag ggcgagctga tcgataggat
     2821 cggcaagctg cagttcaaga cacaggtgga ggagttttac accgcctatt ctaagtacct
     2881 ggtgctgagc tatcagccac agtcccagaa cctgatcgtg aagtacttca gccccggcat
     2941 cgtggatatg ctgaacctga atatgcctaa tggcctggtg gccccaatcg tggacaagga
     3001 tatcttcaag gtgctgacag agcactcccc ttcctctgtg ccacggacct ttaagaacgt
     3061 ggtgagggaa ggcatcaaag caggcagggc cgtgtctgtg gagacaggcc tgctgacagg
     3121 catcgaggag atcaagaagt ctcagaacat cttcagcggc accacagcca atcgggagag
     3181 aggctggaag aaggccgagg agaagtacgt gtgccacttt accccctgta aggatgagat
     3241 gggcagggtc ggctgggtgg tgctgactgt cgctcctaaa ctggaaagat ttgaattcgg
     3301 tggtggatca ggtggaggat ctatggtgag caagggcgag gaggataaca tggccatcat
     3361 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     3421 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     3481 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     3541 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     3601 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     3661 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     3721 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     3781 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     3841 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     3901 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     3961 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     4021 gctgtacaag ggctcttccg ggacaggctc ctccggcact agtcgaaggc gccacatcgt
     4081 tcggaagcgc acgctgcgga ggctgctgca ggagagggag cttgtggagc ctcttacacc
     4141 cagtggagaa gctcccaacc aagctctctt gaggatcttg aaggaaactg aattcaaaaa
     4201 gatcaaagtg ctgggctccg gtgcgttcgg cacggtgtat aagggactct ggatcccaga
     4261 aggtgagaaa gttaaaattc ccgtcgctat caaggaatta agagaagcaa catctccgaa
     4321 agccaacaag gaaatcctcg atgaagccta cgtgatggcc agcgtggaca acccccacgt
     4381 gtgccgcctg ctgggcatct gcctcacctc caccgtgcaa ctcatcacgc agctcatgcc
     4441 cttcggctgc ctcctggact atgtccggga acacaaagac aatattggct cccagtacct
     4501 gctcaactgg tgtgtgcaga tcgcaaaggg catgaactac ttggaggacc gtcgcttggt
     4561 gcaccgcgac ctggcagcca ggaacgtact ggtgaaaaca ccgcagcatg tcaagatcac
     4621 agattttggg ctggccaaac tgctgggtgc ggaagagaaa gaataccatg cagaaggagg
     4681 caaagtgcct atcaagtgga tggcattgga atcaatttta cacagaatct atacccacca
     4741 gagtgatgtc tggagctacg gggtgaccgt ttgggagttg atgacctttg gatccaagcc
     4801 atatgacgga atccctgcca gcgagatctc ctccatcctg gagaaaggag aacgcctccc
     4861 tcagccaccc atatgtacca tcgatgtcta catgatcatg gtcaagtgct ggatgataga
     4921 cgcagatagt cgcccaaagt tccgtgagtt gatcatcgaa ttctccaaaa tggcccgaga
     4981 cccccagcgc taccttgtca ttcaggggga tgaaagaatg catttgccaa gtcctacaga
     5041 ctccaacttc taccgtgccc tgatggatga agaagacatg gacgacgtgg tggatgccga
     5101 cgagtacctc atcccacagc agggcttctt cagcagcccc tccacgtcac ggactcccct
     5161 cctgagctct ctgagtgcaa ccagcaacaa ttccaccgtg gcttgcattg atagaaatgg
     5221 gctgcaaagc tgtcccatca aggaagacag cttcttgcag cgatacagct cagaccccac
     5281 aggcgccttg actgaggaca gcatagacga caccttcctc ccagtgcctg aatacataaa
     5341 ccagtccgtt cccaaaaggc ccgctggctc tgtgcagaat cctgtctatc acaatcagcc
     5401 tctgaacccc gcgcccagca gagacccaca ctaccaggac ccccacagca ctgcagtggg
     5461 caaccccgag tatctcaaca ctgtccagcc cacctgtgtc aacagcacat tcgacagccc
     5521 tgcccactgg gcccagaaag gcagccacca aattagcctg gacaaccctg actaccagca
     5581 ggacttcttt cccaaggaag ccaagccaaa tggcatcttt aagggctcca cagctgaaaa
     5641 tgcagaatac ctaagggtcg cgccacaaag cagtgaattt attggagcat aatctagagg
     5701 gcccgtttaa acccggcggc cgcaactccc acctgcaaca tgcgtgactg actgaggccg
     5761 cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc
     5821 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5881 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5941 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6001 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6061 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6121 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6181 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     6241 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6301 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6361 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgaccaag
     6421 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6481 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6541 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6601 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6661 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta
     6721 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     6781 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
     6841 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
     6901 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
     6961 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     7021 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     7081 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     7141 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     7201 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     7261 agtacaatct agctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     7321 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     7381 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     7441 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     7501 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7561 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7621 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7681 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7741 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7801 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7861 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     7921 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7981 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8041 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8101 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8161 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8221 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8281 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8341 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8401 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8461 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8521 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8581 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8641 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8701 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8761 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     8821 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8881 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8941 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9001 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9061 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9121 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9181 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9241 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9301 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9361 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9421 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9481 ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     9541 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9601 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9661 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9721 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9781 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac
     9841 aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag
     9901 tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa
     9961 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
    10021 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
    10081 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
    10141 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
    10201 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
    10261 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
    10321 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
    10381 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
    10441 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
    10501 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
    10561 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
    10621 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
    10681 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
    10741 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
    10801 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
    10861 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
    10921 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
    10981 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
    11041 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
    11101 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
    11161 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
    11221 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
    11281 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
    11341 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
    11401 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
    11461 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
    11521 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    11581 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    11641 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    11701 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
    11761 aatcatccca aacttcccac cccataccct attaccactg ccaattacct agtggtttca
    11821 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    11881 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
    11941 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
    12001 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
    12061 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
    12121 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
    12181 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
    12241 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
    12301 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
    12361 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    12421 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    12481 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
    12541 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
    12601 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
    12661 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
    12721 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
    12781 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
    12841 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
    12901 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
    12961 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
    13021 acaaaagtaa gaccaccgca cagcaagcgg ccggtgatct tcagacctgg acgatatata
    13081 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
    13141 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
    13201 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
    13261 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
    13321 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
    13381 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
    13441 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
    13501 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
    13561 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
    13621 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
    13681 taacaaattg gctgtggtat ataaaattat tcataa
//
