LOCUS       sequence-405844-        9454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For inserting a phiC31 attP site. HDR integrant is marked with 
            removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed 
            difficult to see in wild type eyes if using LeicaM165 FC 
            microscope..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT7
FEATURES             Location/Qualifiers
     source          1..9454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             127..918
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1164..1752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1653..1672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1906..1923
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1955..1973
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1955..1972
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        2050..2470
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    2477..2600
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2623..2628,2629..2646,2647..2653)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        2696..2800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2801..2869,2870..3661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3019..3038)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4182..4201
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4341..4464)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    4537..4636
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     regulatory      5058..5067
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5247..5267)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     polyA_signal    5852..5986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5889..5908)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5943..5962
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6090..6213
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6236..6241,6242..6259,6260..6266)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6320..6979
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6386..6405)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        6988..7433
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(7434..7520,7521..8855)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(8894..9017)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9109..9128)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9110..9128)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
       61 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      121 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
      181 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
      241 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
      301 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
      361 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
      421 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
      481 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
      541 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
      601 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
      661 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
      721 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
      781 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
      841 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
      901 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc
      961 gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     1021 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     1081 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1141 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1201 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1261 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     1321 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1381 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1441 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1501 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1561 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1621 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1681 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1741 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1801 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1861 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1921 gaggaaatcg tcacctgcag gaaggtttaa acgcatttag gtgacactat agaagtgtgt
     1981 atcgctcgag ggatccgaat tcgaagtctt ggtacggagg ggatccgatc gacaagccga
     2041 acatatgggt tttttgctca cctgtgattg ctcctactca aatacaaaaa catcaaattt
     2101 tctgtcaata aagcatattt atttatattt attttacagg aaagaattcc ttttaaagtg
     2161 tattttaacc tataatgaaa aacgattaaa aaaaatacat aaaataattc gaaaattttt
     2221 gaatagccca ggttgataaa aattcatttc atacgtttta taacttatgc ccctaagtat
     2281 tttttgacca tagtgtttca attctacatt aattttacag agtagaatga aacgccacct
     2341 actcagccaa gaggcgaaaa ggttagctcg ccaagcagag agggcgccag tgctcactac
     2401 tttttataat tctcaacttc tttttccaga ctcagttcgt atatatagac ctattttcaa
     2461 tttaacgtcg gggcctcaac tttgtataat aaagttgaac gagaaacgta aaatgatata
     2521 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
     2581 caacatatcc agtcactatg ggccgcatta ggcaccccag gctttacact ttatgcttcc
     2641 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctacgcgg
     2701 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2761 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2821 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2881 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2941 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3001 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3061 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3121 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3181 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3241 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3301 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3361 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3421 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3481 gtttattgct gataaatctg gagccggtga gcgtgggtcc cgcggtatca ttgcagcact
     3541 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3601 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3661 atggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     3721 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa
     3781 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     3841 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     3901 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     3961 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta
     4021 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta tcgatacacc
     4081 gggccgtcgc atggttattc cacttgcgtc tgcgcgcttg ctctcagata aagtctcccg
     4141 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     4201 ggccagtgtg ccggtttccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     4261 tgacatcaaa aacgccatta acctgatgtt ctggggaata taatgtcagg ctcccttata
     4321 cacagccagt ctgcacgtcg catagtgact ggatatgttg tgttttacag tattatgtag
     4381 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     4441 gttcaacttt tctatacaaa gttgtccagc tgtactgacg gacacaccga agccccggcg
     4501 gcaaccctca gcggatgccc cggggcttca cgttttccca ggtcagaagc ggttttcggg
     4561 agtagtgccc caactggggt aacctttgag ttctctcagt tgggggcgta gggtcgccga
     4621 catgacacaa ggggttgtga ccggggtgga cacgtacgcg ggtgcttacg accgtcagtc
     4681 gcgcgagcgc gaggttgctt aagaataagt aattcttaag caaccattac gccaagcttg
     4741 catgcaggcc tctgcagtcg acgggcccgg gatccgatcg acaagccgaa catatgggcg
     4801 cgccgggatc taattcaatt agagactaat tcaattagag ctaattcaat taggatccaa
     4861 gcttatcgat ttcgaaccct cgaccgccgg agtataaata gaggcgcttc gtctacggag
     4921 cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa
     4981 acaagcgcag ctgaacaagc taaacaatcg gggtaccgct agagtcgacg gtaccgcggg
     5041 cccgggatcc accggtcgcc accatggtgc gctcctccaa gaacgtcatc aaggagttca
     5101 tgcgcttcaa ggtgcgcatg gagggcaccg tgaacggcca cgagttcgag atcgagggcg
     5161 agggcgaggg ccgcccctac gagggccaca acaccgtgaa gctgaaggtg accaagggcg
     5221 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt
     5281 acgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca
     5341 agtgggagcg cgtgatgaat ttcgaagatg gtggagttgt cacagtcaca caagattctt
     5401 cgctccaaga tggttgtttt atttacaaag tgaaatttat tggagtcaat tttccgtcgg
     5461 atggaccggt tatgcaaaag aaaacaatgg gttgggaagc atcgacggaa cgtctgtatc
     5521 cacgagatgg agttttgaaa ggcgaaattc acaaagctct taaactcaaa gatggcggac
     5581 attaccttgt agaatttaaa agcatttata tggctaagaa accggtccaa ctccctggat
     5641 attattatgt cgattcgaaa ctggatatta cttcacataa cgaagattat acgatagttg
     5701 agcaatatga acgcactgaa ggtcgtcacc atctctttct ctaaagatca taatcagcca
     5761 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     5821 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     5881 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5941 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcgatg gcgcgcctcc
     6001 ggaacataat ggtgcatcta gatgcattcg cgaggtaccg agctcgaatt cactggggtt
     6061 gcttaagaat aagtaattct taagcaaccc aactttgtat acaaaagttg aacgagaaac
     6121 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
     6181 atactgtaaa acacaacata tccagtcact atgggccgca ttaggcaccc caggctttac
     6241 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc
     6301 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     6361 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     6421 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     6481 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
     6541 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     6601 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     6661 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     6721 ccctaaaggg tttattgaga atatgttttt cgtgtcagcc aatccctggg tgagtttcac
     6781 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     6841 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     6901 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     6961 gtggcagggc ggggcgtaaa cgcgtggcac atatacctgc cgttcactat tatttagtga
     7021 aatgagatat tatgatattt tctgaattgt gattaaaaag gcaactttat gcccatgcaa
     7081 cagaaactat aaaaaataca gagaatgaaa agaaacagat agatttttta gttctttagg
     7141 cccgtagtct gcaaatcctt ttatgatttt ctatcaaaca aaagaggaaa atagaccagt
     7201 tgcaatccaa acgagagtct aatagaatga ggtcgaaaag taaatcgcgc gggtttgtta
     7261 ctgataaagc aggcaagacc taaaatgtgt aaagggcaaa gtgtatactt tggcgtcacc
     7321 ccttacatat tttaggtctt tttttattgt gcgtaactaa cttgccatct tcaaacagga
     7381 gggctggaag aagcagaccg ctaacacagt acataaaaaa ggagacatga acgatgaaca
     7441 tcaaaaagtt tgcaaaacaa gcaacagtat taacctttac taccgcactg ctggcaggag
     7501 gcgcaactca agcgtttgcg aaagaaacga accaaaagcc atataaggaa acatacggca
     7561 tttcccatat tacacgccat gatatgctgc aaatccctga acagcaaaaa aatgaaaaat
     7621 atcaagttcc tgaattcgat tcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg
     7681 acgtttggga cagctggcca ttacaaaacg ctgacggcac tgtcgcaaac tatcacggct
     7741 accacatcgt ctttgcatta gccggagatc ctaaaaatgc ggatgacaca tcgatttaca
     7801 tgttctatca aaaagtcggc gaaacttcta ttgacagctg gaaaaacgct ggccgcgtct
     7861 ttaaagacag cgacaaattc gatgcaaatg attctatcct aaaagaccaa acacaagaat
     7921 ggtcaggttc agccacattt acatctgacg gaaaaatccg tttattctac actgatttct
     7981 ccggtaaaca ttacggcaaa caaacactga caactgcaca agttaacgta tcagcatcag
     8041 acagctcttt gaacatcaac ggtgtagagg attataaatc aatctttgac ggtgacggaa
     8101 aaacgtatca aaatgtacag cagttcatcg atgaaggcaa ctacagctca ggcgacaacc
     8161 atacgctgag agatcctcac tacgtagaag ataaaggcca caaatactta gtatttgaag
     8221 caaacactgg aactgaagat ggctaccaag gcgaagaatc tttatttaac aaagcatact
     8281 atggcaaaag cacatcattc ttccgtcaag aaagtcaaaa acttctgcaa agcgataaaa
     8341 aacgcacggc tgagttagca aacggcgctc tcggtatgat tgagctaaac gatgattaca
     8401 cactgaaaaa agtgatgaaa ccgctgattg catctaacac agtaacagat gaaattgaac
     8461 gcgcgaacgt ctttaaaatg aacggcaaat ggtacctgtt cactgactcc cgcggatcaa
     8521 aaatgacgat tgacggcatt acgtctaacg atatttacat gcttggttat gtttctaatt
     8581 ctttaactgg cccatacaag ccgctgaaca aaactggcct tgtgttaaaa atggatcttg
     8641 atcctaacga tgtaaccttt acttactcac acttcgctgt acctcaagcg aaaggaaaca
     8701 atgtcgtgat tacaagctat atgacaaaca gaggattcta cgcagacaaa caatcaacgt
     8761 ttgcgccaag cttcctgctg aacatcaaag gcaagaaaac atctgttgtc aaagacagca
     8821 tccttgaaca aggacaatta acagttaaca aataaatgtc aggctccctt atacacagcc
     8881 agtctgcacg tcgcatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
     8941 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac
     9001 tttttaatac aaagttgcct ccggaacata atggtgcatc tagatgccca ttcctgtaga
     9061 cttcttaatt aagacgtcag aattctcgag gcggccgcat gtgagtctcc ctatagtgag
     9121 tcgtattaat ttcgcgggcg cgtcagcggg agagccgttg taaggcggca gactttgctc
     9181 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     9241 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaat
     9301 tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     9361 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     9421 ggcggttttc atggcttgtt atgactgttt tttt
//
