LOCUS       sequence-405845-       14663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express phiC31 under control of D. melanoagaster vasa promoter, with
            Vasa 3'UTR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14663)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 14663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT62
FEATURES             Location/Qualifiers
     source          1..14663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             375..1166
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1412..2000
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1901..1920
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2154..2171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2203..2221
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2203..2220
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        2298..2718
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    2725..2848
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2871..2876,2877..2894,2895..2901)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        2944..3048
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3049..3117,3118..3909)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3267..3286)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4430..4449
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4589..4712)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        4737..5219
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        5223..5814
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     regulatory      5995..6004
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6001..6003,6004..6006,6007..6720)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6001..6003,6004..6006,6007..6717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6046..6067)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6307..6326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6654..6675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6843..6964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6880..6899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6934..6953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6991..7007
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11547..11670
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(11693..11698,11699..11716,11717..11723)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             11777..12436
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(11843..11862)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        12445..12890
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(12891..12977,12978..14312)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(14351..14474)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(14566..14585)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14567..14585)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
       61 taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag tggacataag
      121 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
      181 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
      241 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
      301 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
      361 caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
      421 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
      481 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
      541 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
      601 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
      661 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
      721 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
      781 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
      841 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
      901 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
      961 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     1021 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     1081 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     1141 agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc
     1201 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     1261 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     1321 tgggagatat atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1381 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1441 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1501 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1561 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1621 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1681 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1741 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1801 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1861 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1921 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1981 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2041 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2101 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2161 cagtgagcga ggaaatcgtc acctgcagga aggtttaaac gcatttaggt gacactatag
     2221 aagtgtgtat cgctcgaggg atccgaattc gaagtcttgg tacggagggg atccgatcga
     2281 caagccgaac atatgggttt tttgctcacc tgtgattgct cctactcaaa tacaaaaaca
     2341 tcaaattttc tgtcaataaa gcatatttat ttatatttat tttacaggaa agaattcctt
     2401 ttaaagtgta ttttaaccta taatgaaaaa cgattaaaaa aaatacataa aataattcga
     2461 aaatttttga atagcccagg ttgataaaaa ttcatttcat acgttttata acttatgccc
     2521 ctaagtattt tttgaccata gtgtttcaat tctacattaa ttttacagag tagaatgaaa
     2581 cgccacctac tcagccaaga ggcgaaaagg ttagctcgcc aagcagagag ggcgccagtg
     2641 ctcactactt tttataattc tcaacttctt tttccagact cagttcgtat atatagacct
     2701 attttcaatt taacgtcggg gcctcaactt tgtataataa agttgaacga gaaacgtaaa
     2761 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     2821 gtaaaacaca acatatccag tcactatggg ccgcattagg caccccaggc tttacacttt
     2881 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag
     2941 ctacgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3001 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3061 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3121 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3181 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3241 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     3301 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3361 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     3421 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3481 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3541 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     3601 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3661 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     3721 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtcccg cggtatcatt
     3781 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     3841 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3901 cattggtaat ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     3961 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat
     4021 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
     4081 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     4141 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     4201 ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt
     4261 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatc
     4321 gatacaccgg gccgtcgcat ggttattcca cttgcgtctg cgcgcttgct ctcagataaa
     4381 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     4441 accgatatgg ccagtgtgcc ggtttccgtt atcggggaag aagtggctga tctcagccac
     4501 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata atgtcaggct
     4561 cccttataca cagccagtct gcacgtcgca tagtgactgg atatgttgtg ttttacagta
     4621 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     4681 cgtttctcgt tcaacttttc tatacaaagt tgtccagctc cgagctctta cgcgcccgcg
     4741 taaaacacaa tcaagtatga gtcataagct gatgtcatgt tttgcacacg gctcataacc
     4801 gaactggctt tacgagtaga attctacttg taacgcacga tcagtggatg atgtcatttg
     4861 tttttcaaat cgagatgatg tcatgttttg cacacggctc ataaactcgc tttacgagta
     4921 gaattctacg tgtaacgcac gatcgattga tgagtcattt gttttgcaat atgatatcat
     4981 acaatatgac tcatttgttt ttcaaaaccg aacttgattt acgggtagaa ttctacttgt
     5041 aaagcacaat caaaaagatg atgtcatttg tttttcaaaa ctgaactcgc tttacgagta
     5101 gaattctacg tgtaaaacac aatcaagaaa tgatgtcatt tgttataaaa ataaaagctg
     5161 atgtcatgtt ttgcacatgg ctcataacta aactcgcttt acgggtagaa ttctacgcgc
     5221 gtcgatgtct ttgtgatgcg cgcgacattt ttgtaggtta ttgataaaat gaacggatac
     5281 gttgcccgac attatcatta aatccttggc gtagaatttg tcgggtccat tgtccgtgtg
     5341 cgctagcatg cccgtaacgg acctcgtact tttggcttca aaggttttgc gcacagacaa
     5401 aatgtgccac acttgcagct ctgcatgtgt gcgcgttacc acaaatccca acggcgcagt
     5461 gtacttgttg tatgcaaata aatctcgata aaggcgcggc gcgcgaatgc agctgatcac
     5521 gtacgctcct cgtgttccgt tcaaggacgg tgttatcgac ctcagattaa tgtttatcgg
     5581 ccgactgttt tcgtatccgc tcaccaaacg cgtttttgca ttaacattgt atgtcggcgg
     5641 atgttctata tctaatttga ataaataaac gataaccgcg ttggttttag agggcataat
     5701 aaaagaaata ttgttatcgt gttcgccatt agggcagtat aaattgacgt tcatgttgga
     5761 tattgtttca gttgcaagtt gacactggcg gcgacaagat cgtgaacaac caagtgacca
     5821 ttaaccctcg accgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat
     5881 tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct
     5941 gaacaagcta aacaatcggg gtaccgctag agtcgacggt acgatccacc ggtcgccacc
     6001 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     6061 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     6121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     6181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     6241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     6301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     6361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     6421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     6481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     6541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     6661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     6721 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     6781 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     6841 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6901 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6961 cttaaagctt atcgatacgc gtacggcgcg cgaggtcgac ggtatcgata agcttgatat
     7021 cgaattcctg cagctggttg taggtgcagt tgcgcttctt gcacttggcg cacttgagga
     7081 gatcggtctt ggtgccctgc acggtggcca gctgggcatc gttgatggcc tccttgacga
     7141 acttctcgcg cagcttcttc atctcgtcgc tggccatctc ctccggcgtc attttggcca
     7201 gctgcttggc agtgacggcg ccgcacataa agttgccgcg caatccagga ttcttggggt
     7261 ccttcagatt ggccacgcgt gacctaatgc gattcttgta cttcatatcc gtgttattga
     7321 actcggagta aatggcatcc tccagttcgg cggccatttc ctccggctca ccgcatccct
     7381 cgggcacttc gccaatcttc agtgcggtgg ccagcatttc gcggcatttg atgcggaccg
     7441 catctgtcat gccaccggag ggaaacgagg tctgtgacga ggatgtcgag cctttcttct
     7501 ctttatcctt ggatgagctg gatgaactgg acttatcctt gccggagatt gacgacgacg
     7561 acttggcggc actggtggac ttcgaggcac tgctgttgtt ggaggagccc tccttagcgg
     7621 agctgttgtt cggtgtggtt ggcgccgggc tggccaggaa gcgtttccag ttcttgatca
     7681 gcgtcttggc cagagcgatc acctcgtcgt ccttgctact cttgcgcagc tcgtttacgg
     7741 tcatgccgat gcgcgttttg gtcagaatgt cgagattgat gttaagcgtt tgcagggcct
     7801 tcagcaggtc cagagcctga tcctgtccct gtacggtgga tatatgtata tttgtgacat
     7861 gatgcgcggc acgttagcta atcccgatac ttactgtgcc gtcgctggcc atcttgctca
     7921 tcttcttttg gattcgaaac acttcctcct ccacgctcat tttggcccac ttaatttgat
     7981 cagaggttag gttaactggc gtcggctgtc ggctttaatc ggtgctgcgg gtctgcgaca
     8041 gttgtttatg cggaagccgg gctatttctt tttttccctc caataaatgt tgtttactat
     8101 ttttttcgtt tctgtattcg cagaacaagt tgacagaaca ccctaacgcg ttttagtgtt
     8161 accgggcggt ggcttgtcaa gaaatacctt cccatggcta atacaattcc caactggaag
     8221 tagtttgggt ttcagaagtc aacagtgtta ccgtatttta atgtacatct ggcttttaag
     8281 cgagtacaca cacatagcat tgcgatgttt tttaaacttg cactgcttgc aagcaggggt
     8341 ccactgtatt tcaatgaaag aacagcgtgt tgggcgtttt tgaattatac taattacctt
     8401 caataacaat tccctatata tcacttagtt ttaataaata aatcgtttga gtgtggtcta
     8461 gagatgattt attatgatga agtgcggttg ttggggacaa gcgggatacg tctgcgggtc
     8521 aaatcggcgg tgggacatag tgagagggca acggcgaaca atcaccaacg cgccctggta
     8581 cgcgagaaat gttgatatcg cacatgacct cagcatccac cttcagtttg agaacaaatt
     8641 aaatcaggca cataccagac aaccaggaga acactgagga ggcgtcatca aagatatctt
     8701 tggacacgtg gcataaacaa gccaaactat tatctaatca atatttttat acaaattttg
     8761 tatgtttcca tgttatcttc gttgctcctg ttagttacta cgtttcatag cccttaaatt
     8821 ggttgcttaa cgtaaaataa aattatattg tataaaaata aagaggaatt cacctataac
     8881 aaacgaaaaa gtaaataaat aagaacttcc ccatgttaaa aatgccacca ccatctttac
     8941 atataagtat gtacatatga atgaatcact taggttgctt gaatattaca atttcattaa
     9001 tagctaaatc acatttgatg tgttagtgga aaacggctat atatataaat tatcgaaatt
     9061 gtgaatatcg aattgcgata gcacaatggg aaattccacc actagatttt tggtactttt
     9121 aacagatcct tttcggtttt gcgttgcgcg aagtgatctg aacttatcaa agtttgtaag
     9181 gtaatacata aagtgaaaaa gaattaattt gctcttgaaa ggcaggccaa attaaaaaaa
     9241 aaatatcaat atggacacgt atgccggtgc ttacgaccgt cagagccgcg agcgggaaaa
     9301 ttccagcgca gcaagtccag ccacacagcg tagcgccaac gaggacaagg cggccgactt
     9361 gcagcgagaa gtcgagcgag atggtggcag gttcaggttc gtcgggcatt tctccgaagc
     9421 tccaggcact agcgcgttcg gaacagccga gcgcccggag ttcgaacgca tcctgaacga
     9481 gtgccgtgcc ggcaggctca acatgatcat agtctatgac gtgagtcgct tctcgcgcct
     9541 gaaggtcatg gacgcgattc cgattgtgag cgaattgctc gccctgggtg tgacgatagt
     9601 gtccacgcag gaaggcgtgt ttcggcaggg aaacgtcatg gacctgatac acctgattat
     9661 gcgcttggac gcttcgcaca aggagtccag cctgaagagt gcgaagattc tcgacaccaa
     9721 gaacctgcag cgcgaattgg gcggttatgt cggcggtaag gctccctacg gcttcgagct
     9781 tgtttcggag acgaaggaga tcacgcgtaa cggccgaatg gtcaatgtcg tgatcaacaa
     9841 gcttgcccac tcgaccacac cccttacccg acccttcgag ttcgagcccg acgttatccg
     9901 ttggtggtgg cgtgagatca agacccacaa gcaccttccc ttcaagccag gcagtcaagc
     9961 cgccattcac ccgggcagca ttacggggct gtgtaagcgc atggacgcag atgctgtgcc
    10021 aacaaggggt gagacgattg gcaagaagac cgcttcaagc gcctgggacc ccgcaaccgt
    10081 tatgcgaatc cttcgtgacc cgcgtattgc aggctttgcc gctgaggtga tctacaagaa
    10141 gaagccggat ggaacgccga ccacgaagat agagggttac cgcattcagc gcgatccgat
    10201 caccttgcgc cccgtggagc tcgattgcgg acccatcatc gagcccgctg agtggtatga
    10261 gctgcaggcg tggttggatg gcaggggtcg cggcaagggt ctttcccggg gacaagccat
    10321 actgagcgcc atggacaagc tgtactgcga atgtggcgcc gtgatgactt cgaagcgcgg
    10381 cgaggaatcg atcaaggact cctaccgctg ccgtcgacgg aaggtggttg acccaagcgc
    10441 acctggtcag catgagggca cgtgcaacgt gagcatggcg gcactcgaca agttcgttgc
    10501 ggaacgcatc ttcaacaaga tcaggcacgc tgaaggcgac gaagagacgt tggcccttct
    10561 gtgggaagcc gcgcgacgct tcggcaagct cactgaggcg ccagagaagt ccggcgaacg
    10621 ggcgaacctt gttgcggagc gagccgatgc cttgaacgcc ctcgaagagc tgtacgaaga
    10681 ccgcgctgca ggagcttacg acggacccgt tggcaggaag cacttccgga agcaacaggc
    10741 agcgctgaca ctccggcagc aaggagcaga agagcgactt gccgagcttg aagccgccga
    10801 agccccaaag ctccccttgg accaatggtt ccccgaagac gccgacgctg acccaactgg
    10861 accgaagtcg tggtggggac gcgcatcagt ggacgacaag cgcgtgtttg tgggcctctt
    10921 cgtggacaag atcgttgtga ccaagagcac taccggcagg ggacagggaa cacccatcga
    10981 gaagcgcgct tcgatcacgt gggcaaagcc cccgaccgat gacgatgaag atgacgccca
    11041 ggatggcacc gaagacgtag cggcctagct cgagaatgta tggacataga tttcaaataa
    11101 ttaaatgtaa tgcagtaatt gatgtaatta gttaaataag ttagatatta ataacatatt
    11161 aattatatgt attataacgc atataataat aaaatgcata tttaggatat gcaagccatt
    11221 cgaattttct attttaattt ccttttacaa agaaatgtat aacaaaatat aatttgaaaa
    11281 aatgttctgg ctctaattcg atttctttta agtattttgt gaactgcttt taataacgag
    11341 cggttgcaaa acttaacaga aacgtgcact ttgatcccaa ctaataccgt gtactatcac
    11401 gtgtttggtt ttaagtactc atccttcgtg tttcgttgtt tgttgtttgc cttgctgtac
    11461 acttggctct tgcgctctct cgctctccgt tggagccggc tttttgaatg catgcctcgc
    11521 cctgctcgcg cccagtcctc ctccatcaac tttgtataca aaagttgaac gagaaacgta
    11581 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
    11641 ctgtaaaaca caacatatcc agtcactatg ggccgcatta ggcaccccag gctttacact
    11701 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
    11761 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
    11821 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
    11881 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
    11941 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
    12001 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
    12061 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
    12121 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
    12181 taaagggttt attgagaata tgtttttcgt gtcagccaat ccctgggtga gtttcaccag
    12241 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
    12301 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
    12361 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
    12421 gcagggcggg gcgtaaacgc gtggcacata tacctgccgt tcactattat ttagtgaaat
    12481 gagatattat gatattttct gaattgtgat taaaaaggca actttatgcc catgcaacag
    12541 aaactataaa aaatacagag aatgaaaaga aacagataga ttttttagtt ctttaggccc
    12601 gtagtctgca aatcctttta tgattttcta tcaaacaaaa gaggaaaata gaccagttgc
    12661 aatccaaacg agagtctaat agaatgaggt cgaaaagtaa atcgcgcggg tttgttactg
    12721 ataaagcagg caagacctaa aatgtgtaaa gggcaaagtg tatactttgg cgtcacccct
    12781 tacatatttt aggtcttttt ttattgtgcg taactaactt gccatcttca aacaggaggg
    12841 ctggaagaag cagaccgcta acacagtaca taaaaaagga gacatgaacg atgaacatca
    12901 aaaagtttgc aaaacaagca acagtattaa cctttactac cgcactgctg gcaggaggcg
    12961 caactcaagc gtttgcgaaa gaaacgaacc aaaagccata taaggaaaca tacggcattt
    13021 cccatattac acgccatgat atgctgcaaa tccctgaaca gcaaaaaaat gaaaaatatc
    13081 aagttcctga attcgattcg tccacaatta aaaatatctc ttctgcaaaa ggcctggacg
    13141 tttgggacag ctggccatta caaaacgctg acggcactgt cgcaaactat cacggctacc
    13201 acatcgtctt tgcattagcc ggagatccta aaaatgcgga tgacacatcg atttacatgt
    13261 tctatcaaaa agtcggcgaa acttctattg acagctggaa aaacgctggc cgcgtcttta
    13321 aagacagcga caaattcgat gcaaatgatt ctatcctaaa agaccaaaca caagaatggt
    13381 caggttcagc cacatttaca tctgacggaa aaatccgttt attctacact gatttctccg
    13441 gtaaacatta cggcaaacaa acactgacaa ctgcacaagt taacgtatca gcatcagaca
    13501 gctctttgaa catcaacggt gtagaggatt ataaatcaat ctttgacggt gacggaaaaa
    13561 cgtatcaaaa tgtacagcag ttcatcgatg aaggcaacta cagctcaggc gacaaccata
    13621 cgctgagaga tcctcactac gtagaagata aaggccacaa atacttagta tttgaagcaa
    13681 acactggaac tgaagatggc taccaaggcg aagaatcttt atttaacaaa gcatactatg
    13741 gcaaaagcac atcattcttc cgtcaagaaa gtcaaaaact tctgcaaagc gataaaaaac
    13801 gcacggctga gttagcaaac ggcgctctcg gtatgattga gctaaacgat gattacacac
    13861 tgaaaaaagt gatgaaaccg ctgattgcat ctaacacagt aacagatgaa attgaacgcg
    13921 cgaacgtctt taaaatgaac ggcaaatggt acctgttcac tgactcccgc ggatcaaaaa
    13981 tgacgattga cggcattacg tctaacgata tttacatgct tggttatgtt tctaattctt
    14041 taactggccc atacaagccg ctgaacaaaa ctggccttgt gttaaaaatg gatcttgatc
    14101 ctaacgatgt aacctttact tactcacact tcgctgtacc tcaagcgaaa ggaaacaatg
    14161 tcgtgattac aagctatatg acaaacagag gattctacgc agacaaacaa tcaacgtttg
    14221 cgccaagctt cctgctgaac atcaaaggca agaaaacatc tgttgtcaaa gacagcatcc
    14281 ttgaacaagg acaattaaca gttaacaaat aaatgtcagg ctcccttata cacagccagt
    14341 ctgcacgtcg catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
    14401 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt
    14461 ttaatacaaa gttgcctccg gaacataatg gtgcatctag atgcccattc ctgtagactt
    14521 cttaattaag acgtcagaat tctcgaggcg gccgcatgtg agtctcccta tagtgagtcg
    14581 tattaatttc gcgggcgcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
    14641 ttaccgatgc tattcggaag aac
//
