LOCUS       sequence-405846-       14671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked 
            with removable ie1XP3-DsRed cassette expressed in abdomen and 
            mouthparts..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14671)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 14671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT71
FEATURES             Location/Qualifiers
     source          1..14671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    109..203
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    237..331
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        356..594
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     541..562
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          609..675
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             714..731
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(771..779,780..794)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             2055..2108
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(4255..4273)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    4512..4646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4536..4555)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4590..4609
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(5623..6214)
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     protein_bind    6538..6661
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6684..6689,6690..6707,6708..6714)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6768..7427
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6834..6853)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        7436..7881
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(7882..7968,7969..9303)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(9342..9465)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9557..9576)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9558..9576)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             10029..10820
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      11066..11654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11555..11574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11808..11825
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        11857..11875
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     11857..11874
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        11952..12372
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    12379..12502
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(12525..12530,12531..12548,12549..12555)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        12598..12702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12703..12771,12772..13563)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12921..12940)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     14084..14103
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(14243..14366)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    14505..14599
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    14524..14618
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    join(14652..14671,1..75)
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
       61 gtactgtcct ccgagcggag actctagcgc tagcgcatgc ctgcaggtcg gagtactgtc
      121 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      181 cgagcggagt actgtcctcc gagcggagac tctagcacta gtgcatgcct gcaggtcgga
      241 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
      301 ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgacgtc gagcgccgga
      361 gtataaatag aggcgcttcg tctacggagc gacaattcaa ttcaaacaag caaagtgaac
      421 acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc tgaacaagct aaacaatctg
      481 cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc agcaaccaag taaatcaact
      541 gcaactactg aaatctgcca agaagtaatt attgaataca agaagagaac tctgaataga
      601 tctaaaaggt aggttcaacc actgatgcct aggcacaccg aaacgactaa ccctaattct
      661 tatcctttac ttcaggcggc cgcaacttaa aaaaaaaaat caaaatgggc tcacatcatc
      721 accaccacca cggaatggct agcatgacgg gcggacaaca gatgggccgc gatctgtacg
      781 atgatgatga taaggacctg gcgacaatgg ttgacagcag ccggcgcaag tggaacaaaa
      841 caggacatgc agtcagagtc attggccgcc tttcctcact ggaaaacgtc tacataaagg
      901 cggataagca gaagaacggt atcaaagcca atttccatat ccgccacaac atcgaagatg
      961 gcggagtgca gctcgcatac cactaccaac agaatacgcc aatcggagac ggccccgtgc
     1021 tgctgcccga caaccattac ctgagtgttc agtcgaagct tagcaaggac ccaaacgaaa
     1081 aacgagatca tatggttctg ctggaatttg tgactgctgc tggtattacc ctgggcatgg
     1141 atgaactcta taagggtgga acgggaggct ccatggttag caaaggcgag gagctgttca
     1201 ctggcgtcgt gccgatcctg gtcgagttgg acggagatgt taatggacac aaattcagtg
     1261 tgagtggcga gggcgagggc gatgcgacat acggcaagtt gaccttgaag ttcatttgca
     1321 ccaccggtaa actgccagta ccctggccca cgctggtcac cacgctcacc tatggcgtcc
     1381 agtgcttctc tcgttatccg gatcacatga agcagcatga tttcttcaag tccgccatgc
     1441 ctgaggggta cattcaagag cgaacaatct tcttcaagga cgacggcaat tacaagacca
     1501 gagccgaagt gaagtttgaa ggcgatacac tagtgaaccg catcgagcta aagggtattg
     1561 attttaagga ggatggcaac atcttgggcc acaagctgga gtacaacctt cccgatcagc
     1621 taaccgagga gcagatcgcg gagttcaagg aactgttttc gctgttcgac aaagatggtg
     1681 atggtacgat taccacgaag gaattgggaa cagtgatgcg gtccctgggg cagaacccca
     1741 ccgaggccga gctgcaggat atgataaacg aggtggatgc tgatggtgac ggcacgattg
     1801 attttcccga gttcttgacc atgatggccc gaaagatgaa gtaccgcgat acagaggagg
     1861 agattaggga ggcattcggt gtgttcgaca aggacggcaa tggatacatt agtgcagcag
     1921 agttgcgcca cgttatgact aacctgggtg agaagttgac tgacgaagag gtcgatgaga
     1981 tgattaggga agccgatatt gacggcgatg gccaggtgaa ctatgaggaa ttcgtacaaa
     2041 tgatgaccgc caaagagggt cgcggatcgc tgctgacctg cggcgacgtg gaggaaaacc
     2101 ccggccccgt tagcaagggt gaagaggtga ttaaagaatt catgcgtttt aaagtacgca
     2161 tggagggtag catgaacggt catgagtttg agatcgaggg cgaaggcgaa ggtcgtccgt
     2221 acgagggtac gcagaccgct aaactgaaag taaccaaagg cggtcctctg ccgttcgcgt
     2281 gggacatcct gtccccgcag tttatgtatg gttccaaggc ctacgtcaaa cacccagccg
     2341 atatcccgga ctacaagaag ctgtctttcc cggagggttt caagtgggag cgtgtaatga
     2401 actttgagga cggtggcctg gttaccgtca cccaggatag ctctctgcaa gatggcaccc
     2461 tgatctacaa ggtgaagatg cgtggtacga actttccgcc ggatggcccg gtgatgcaga
     2521 aaaagacgat gggatgggaa gcatctaccg agcgcctgta tccgcgtgac ggtgttctga
     2581 aaggtgagat ccaccaggcg ctgaaactga aggacggcgg tcactacctg gttgaattta
     2641 agacgatcta tatggctaaa aagccggttc aactgccagg ttactactac gtggacacca
     2701 aactggatat cacgtctcac aacgaagatt ataccatcgt cgaacagtat gaacgtagcg
     2761 agggccgtca ccacctgttc ctgggccacg gtactggttc tacgggttct ggttcctctg
     2821 gcactgcgtc ttccgaagac aacaacatgg cggtgatcaa agagttcatg aggttcaaag
     2881 tacgtatgga aggttctatg aacggccatg agttcgagat tgaaggtgag ggcgagggtc
     2941 gcccatacga gggcactcag accgcaaagc tgaaagttac caaaggtggc ccactgcctt
     3001 ttgcgtggga tatcctgtct ccgcagttca tgtacggttc caaagcctac gtaaaacacc
     3061 cggcagacat cccggattac aaaaagctgt ccttcccaga gggcttcaaa tgggaacgtg
     3121 ttatgaattt cgaagatggc ggcctggtca ctgttacgca ggacagcagc ctgcaagacg
     3181 gtactctgat ttacaaagtg aaaatgcgtg gcactaactt cccgcctgac ggtccagtaa
     3241 tgcagaagaa aactatgggc tgggaagcta gcaccgaacg tctgtacccg cgcgacggtg
     3301 tgctgaaagg cgagattcac caggccctga agctgaagga tggtggtcac tatctggttg
     3361 agttcaaaac tatctacatg gcaaaaaagc ctgtacaact gccgggctat tactacgttg
     3421 acactaaact ggacatcacc tcccacaacg aggattatac tattgttgaa cagtacgagc
     3481 gttctgaagg tcgccatcat ctgtttctgt atggtatgga cgaactgtat aaataaggtg
     3541 tacaaatcta gaatgaatcg tttttaaaat aacaaatcaa ttgttttata atattcgtac
     3601 gattctttga ttatgtaata aaatgtgatc attaggaaga ttacgaaaaa tataaaaaat
     3661 atgagttctg tgtgtataac aaatgctgta aacgccacaa ttgtgtttgt tgcaaataaa
     3721 cccatgatta tttgattaaa attgttgttt tctttgttca tagacaatag tgtgttttgc
     3781 ctaaacgtgt actgcataaa ctccatgcga gtgtatagcg agctagtggc taacgcttgc
     3841 cccaccaaag tagattcgtc aaaatcctca atttcatcac cctcctccaa gtttaacatt
     3901 tggccgtcgg aattaacttc taaagatgcc acataatcta ataaatgaaa tagagattca
     3961 aacgtggcgt catcgtccgt ttcgaccatt tccgaaaaga actcgggcat aaactctatg
     4021 atttctctgg acgtggtgtt gtcgaaactc tcaaagtacg cagtcaggaa cgtgcgcgac
     4081 atgtcgtcgg gaaactcgcg cggaaacatg ttgttgtaac cgaacgggtc ccatagcgcc
     4141 aaaaccaaat ctgccagcgt caatagaatg agcacgatgc cgacaatgga gctggcttgg
     4201 atagcgattc gagttaacgg ccggccgttt aaacgaattc ttgaagacga aagggcctcg
     4261 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag actcaggtgg
     4321 cacttttcgg ggaaaggttg cttaagaata agtaattctt aagcaaccat tacgccaagc
     4381 ttgcatgcag gcctctgcag tcgacgggcc cgggatccga tcgacaagcc gaacatatgg
     4441 gccagtgaat tcgagctcgg tacctcgcga atgcatctag atgcaccatt atgttccgga
     4501 ggcgcgccat cgatccagac atgataagat acattgatga gtttggacaa accacaacta
     4561 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     4621 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     4681 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     4741 ctgattatga tctttagaga aagagatggt gacgaccttc agtgcgttca tattgctcaa
     4801 ctatcgtata atcttcgtta tgtgaagtaa tatccagttt cgaatcgaca taataatatc
     4861 cagggagttg gaccggtttc ttagccatat aaatgctttt aaattctaca aggtaatgtc
     4921 cgccatcttt gagtttaaga gctttgtgaa tttcgccttt caaaactcca tctcgtggat
     4981 acagacgttc cgtcgatgct tcccaaccca ttgttttctt ttgcataacc ggtccatccg
     5041 acggaaaatt gactccaata aatttcactt tgtaaataaa acaaccatct tggagcgaag
     5101 aatcttgtgt gactgtgaca actccaccat cttcgaaatt catcacgcgc tcccacttga
     5161 agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc ttcacgtaca
     5221 ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc agggggccgc
     5281 ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc tcgccctcgc
     5341 cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg aagcgcatga
     5401 actccttgat gacgttcttg gaggagcgca ccatggggtg gcgaccggtg gatcgtaccg
     5461 tcgactctag cggtaccccg attgtttagc ttgttcagct gcgcttgttt atttgcttag
     5521 ctttcgctta gcgacgtgtt cactttgctt gtttgaattg aattgtcgct ccgtagacga
     5581 agcgcctcta tttatactcc ggcggtcgag ggttaatggt cacttggttg ttcacgatct
     5641 tgtcgccgcc agtgtcaact tgcaactgaa acaatatcca acatgaacgt caatttatac
     5701 tgccctaatg gcgaacacga taacaatatt tcttttatta tgccctctaa aaccaacgcg
     5761 gttatcgttt atttattcaa attagatata gaacatccgc cgacatacaa tgttaatgca
     5821 aaaacgcgtt tggtgagcgg atacgaaaac agtcggccga taaacattaa tctgaggtcg
     5881 ataacaccgt ccttgaacgg aacacgagga gcgtacgtga tcagctgcat tcgcgcgccg
     5941 cgcctttatc gagatttatt tgcatacaac aagtacactg cgccgttggg atttgtggta
     6001 acgcgcacac atgcagagct gcaagtgtgg cacattttgt ctgtgcgcaa aacctttgaa
     6061 gccaaaagta cgaggtccgt tacgggcatg ctagcgcaca cggacaatgg acccgacaaa
     6121 ttctacgcca aggatttaat gataatgtcg ggcaacgtat ccgttcattt tatcaataac
     6181 ctacaaaaat gtcgcgcgca tcacaaagac atcgacgcgc gtagaattct acccgtaaag
     6241 cgagtttagt tatgagccat gtgcaaaaca tgacatcatc tcgatttgaa aaacaaatga
     6301 catcatccac tgatcgtgcg ttacaagtag aattctactc gtaaagccag ttcggttatg
     6361 agccgtgtgc aaaacatgac atcagcttat gactcatact tgattgtgtt ttacgcgggc
     6421 gcgtaagagc tcggcctccg gaacataatg gtgcatctag atgcattcgc gaggtaccga
     6481 gctcgaattc actggggttg cttaagaata agtaattctt aagcaaccca accccatcaa
     6541 ctttgtatac aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     6601 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     6661 gggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
     6721 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     6781 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     6841 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     6901 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     6961 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
     7021 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     7081 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     7141 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     7201 tgtcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     7261 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     7321 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     7381 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggcacat
     7441 atacctgccg ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga
     7501 ttaaaaaggc aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag
     7561 aaacagatag attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct
     7621 atcaaacaaa agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg
     7681 tcgaaaagta aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa
     7741 agggcaaagt gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc
     7801 gtaactaact tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac
     7861 ataaaaaagg agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta
     7921 acctttacta ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac
     7981 caaaagccat ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa
     8041 atccctgaac agcaaaaaaa tgaaaaatat caagttcctg aattcgattc gtccacaatt
     8101 aaaaatatct cttctgcaaa aggcctggac gtttgggaca gctggccatt acaaaacgct
     8161 gacggcactg tcgcaaacta tcacggctac cacatcgtct ttgcattagc cggagatcct
     8221 aaaaatgcgg atgacacatc gatttacatg ttctatcaaa aagtcggcga aacttctatt
     8281 gacagctgga aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat
     8341 tctatcctaa aagaccaaac acaagaatgg tcaggttcag ccacatttac atctgacgga
     8401 aaaatccgtt tattctacac tgatttctcc ggtaaacatt acggcaaaca aacactgaca
     8461 actgcacaag ttaacgtatc agcatcagac agctctttga acatcaacgg tgtagaggat
     8521 tataaatcaa tctttgacgg tgacggaaaa acgtatcaaa atgtacagca gttcatcgat
     8581 gaaggcaact acagctcagg cgacaaccat acgctgagag atcctcacta cgtagaagat
     8641 aaaggccaca aatacttagt atttgaagca aacactggaa ctgaagatgg ctaccaaggc
     8701 gaagaatctt tatttaacaa agcatactat ggcaaaagca catcattctt ccgtcaagaa
     8761 agtcaaaaac ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa cggcgctctc
     8821 ggtatgattg agctaaacga tgattacaca ctgaaaaaag tgatgaaacc gctgattgca
     8881 tctaacacag taacagatga aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg
     8941 tacctgttca ctgactcccg cggatcaaaa atgacgattg acggcattac gtctaacgat
     9001 atttacatgc ttggttatgt ttctaattct ttaactggcc catacaagcc gctgaacaaa
     9061 actggccttg tgttaaaaat ggatcttgat cctaacgatg taacctttac ttactcacac
     9121 ttcgctgtac ctcaagcgaa aggaaacaat gtcgtgatta caagctatat gacaaacaga
     9181 ggattctacg cagacaaaca atcaacgttt gcgccaagct tcctgctgaa catcaaaggc
     9241 aagaaaacat ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa
     9301 taaatgtcag gctcccttat acacagccag tctgcacgtc gcatagtgac tggatatgtt
     9361 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     9421 ttatatcatt ttacgtttct cgttcaactt tttaatacaa agttgcctcc ggaacataat
     9481 ggtgcatcta gatgcccatt cctgtagact tcttaattaa gacgtcagaa ttctcgaggc
     9541 ggccgcatgt gagtctccct atagtgagtc gtattaattt cgcgggcgcg tcagcgggag
     9601 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
     9661 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
     9721 tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt
     9781 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     9841 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     9901 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     9961 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
    10021 taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
    10081 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
    10141 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
    10201 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
    10261 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
    10321 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
    10381 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
    10441 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
    10501 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
    10561 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
    10621 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
    10681 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
    10741 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
    10801 ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg
    10861 gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc
    10921 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag
    10981 atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    11041 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    11101 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    11161 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
    11221 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    11281 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    11341 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11401 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11461 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    11521 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    11581 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11641 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11701 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11761 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    11821 gcgaggaaat cgtcacctgc aggaaggttt aaacgcattt aggtgacact atagaagtgt
    11881 gtatcgctcg agggatccga attcgaagtc ttggtacgga ggggatccga tcgacaagcc
    11941 gaacatatgg gttttttgct cacctgtgat tgctcctact caaatacaaa aacatcaaat
    12001 tttctgtcaa taaagcatat ttatttatat ttattttaca ggaaagaatt ccttttaaag
    12061 tgtattttaa cctataatga aaaacgatta aaaaaaatac ataaaataat tcgaaaattt
    12121 ttgaatagcc caggttgata aaaattcatt tcatacgttt tataacttat gcccctaagt
    12181 attttttgac catagtgttt caattctaca ttaattttac agagtagaat gaaacgccac
    12241 ctactcagcc aagaggcgaa aaggttagct cgccaagcag agagggcgcc agtgctcact
    12301 actttttata attctcaact tctttttcca gactcagttc gtatatatag acctattttc
    12361 aatttaacgt cggggcctca actttgtata ataaagttga acgagaaacg taaaatgata
    12421 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
    12481 cacaacatat ccagtcacta tgggccgcat taggcacccc aggctttaca ctttatgctt
    12541 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctacgc
    12601 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
    12661 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
    12721 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
    12781 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
    12841 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
    12901 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
    12961 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
    13021 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
    13081 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
    13141 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
    13201 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
    13261 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
    13321 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
    13381 tggtttattg ctgataaatc tggagccggt gagcgtgggt cccgcggtat cattgcagca
    13441 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    13501 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    13561 taatggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
    13621 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg
    13681 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
    13741 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
    13801 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
    13861 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc
    13921 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tatcgataca
    13981 ccgggccgtc gcatggttat tccacttgcg tctgcgcgct tgctctcaga taaagtctcc
    14041 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
    14101 atggccagtg tgccggtttc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
    14161 aatgacatca aaaacgccat taacctgatg ttctggggaa tataatgtca ggctccctta
    14221 tacacagcca gtctgcacgt cgcatagtga ctggatatgt tgtgttttac agtattatgt
    14281 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
    14341 tcgttcaact tttctataca aagttgtcca gctggaggct cgcgtatcgg tgattcattc
    14401 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
    14461 atgcgcaccc gtggccaggg ccgcaagctt gcatgcctgc aggtcggagt actgtcctcc
    14521 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
    14581 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagactctag ccctagggca
    14641 tgcctgcagg tcggagtact gtcctccgag c
//
