LOCUS       sequence-405848-        9620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating targeting inversions. N-terminal split GFP, expressed
            in flight muscles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 9620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT18
FEATURES             Location/Qualifiers
     source          1..9620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    627..748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(664..683)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     718..737
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    780..903
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(926..931,932..949,950..956)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1010..1669
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1076..1095)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        1678..2123
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(2124..2210,2211..3545)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(3584..3707)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3799..3818)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3800..3818)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4271..5062
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5308..5896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5797..5816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6050..6067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        6099..6117
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6099..6116
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        6194..6614
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    6621..6744
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6767..6772,6773..6790,6791..6797)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        6840..6944
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6945..7013,7014..7805)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7163..7182)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     8324..8343
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8483..8606)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctggtgacc
       61 accctgacct acggcgtgca gtgcttcagc cgctactccg atcacatgaa gcgccacgat
      121 ttcttcaaga gcgccatgcc cgagggctac gtgcaggagc gcaccatcag cttcaaggat
      181 gatggcaagt acaagacccg cgccgtggtg aagttcgagg gcgataccct ggtgaaccgc
      241 atcgagctga agggcaccga tttcaaggag gatggcaaca tcctgggcca caagctggag
      301 tacaacttca acagccacaa cgtgtacatc accgccgata agcagaagaa cggcatcaag
      361 gccaacttca ccgtgcgcca caacgtggag gatggcagcg tgcagctggc cgatcactac
      421 cagcagaaca cccccatcgg cgatggcccc gtgctgctgc ccgataacca ctacctgagc
      481 acccagaccg tgctgagcaa ggatcccaac gagaagtaaa gatcataatc agccatacca
      541 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
      601 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
      661 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
      721 gtttgtccaa actcatcaat gtatcttaaa gcttatcgat acgcccatgg tctcaccatc
      781 aactttgtat acaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa
      841 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
      901 atgggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
      961 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     1021 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     1081 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     1141 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     1201 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
     1261 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     1321 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     1381 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     1441 cgtgtcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     1501 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     1561 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     1621 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggcac
     1681 atatacctgc cgttcactat tatttagtga aatgagatat tatgatattt tctgaattgt
     1741 gattaaaaag gcaactttat gcccatgcaa cagaaactat aaaaaataca gagaatgaaa
     1801 agaaacagat agatttttta gttctttagg cccgtagtct gcaaatcctt ttatgatttt
     1861 ctatcaaaca aaagaggaaa atagaccagt tgcaatccaa acgagagtct aatagaatga
     1921 ggtcgaaaag taaatcgcgc gggtttgtta ctgataaagc aggcaagacc taaaatgtgt
     1981 aaagggcaaa gtgtatactt tggcgtcacc ccttacatat tttaggtctt tttttattgt
     2041 gcgtaactaa cttgccatct tcaaacagga gggctggaag aagcagaccg ctaacacagt
     2101 acataaaaaa ggagacatga acgatgaaca tcaaaaagtt tgcaaaacaa gcaacagtat
     2161 taacctttac taccgcactg ctggcaggag gcgcaactca agcgtttgcg aaagaaacga
     2221 accaaaagcc atataaggaa acatacggca tttcccatat tacacgccat gatatgctgc
     2281 aaatccctga acagcaaaaa aatgaaaaat atcaagttcc tgaattcgat tcgtccacaa
     2341 ttaaaaatat ctcttctgca aaaggcctgg acgtttggga cagctggcca ttacaaaacg
     2401 ctgacggcac tgtcgcaaac tatcacggct accacatcgt ctttgcatta gccggagatc
     2461 ctaaaaatgc ggatgacaca tcgatttaca tgttctatca aaaagtcggc gaaacttcta
     2521 ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag cgacaaattc gatgcaaatg
     2581 attctatcct aaaagaccaa acacaagaat ggtcaggttc agccacattt acatctgacg
     2641 gaaaaatccg tttattctac actgatttct ccggtaaaca ttacggcaaa caaacactga
     2701 caactgcaca agttaacgta tcagcatcag acagctcttt gaacatcaac ggtgtagagg
     2761 attataaatc aatctttgac ggtgacggaa aaacgtatca aaatgtacag cagttcatcg
     2821 atgaaggcaa ctacagctca ggcgacaacc atacgctgag agatcctcac tacgtagaag
     2881 ataaaggcca caaatactta gtatttgaag caaacactgg aactgaagat ggctaccaag
     2941 gcgaagaatc tttatttaac aaagcatact atggcaaaag cacatcattc ttccgtcaag
     3001 aaagtcaaaa acttctgcaa agcgataaaa aacgcacggc tgagttagca aacggcgctc
     3061 tcggtatgat tgagctaaac gatgattaca cactgaaaaa agtgatgaaa ccgctgattg
     3121 catctaacac agtaacagat gaaattgaac gcgcgaacgt ctttaaaatg aacggcaaat
     3181 ggtacctgtt cactgactcc cgcggatcaa aaatgacgat tgacggcatt acgtctaacg
     3241 atatttacat gcttggttat gtttctaatt ctttaactgg cccatacaag ccgctgaaca
     3301 aaactggcct tgtgttaaaa atggatcttg atcctaacga tgtaaccttt acttactcac
     3361 acttcgctgt acctcaagcg aaaggaaaca atgtcgtgat tacaagctat atgacaaaca
     3421 gaggattcta cgcagacaaa caatcaacgt ttgcgccaag cttcctgctg aacatcaaag
     3481 gcaagaaaac atctgttgtc aaagacagca tccttgaaca aggacaatta acagttaaca
     3541 aataaatgtc aggctccctt atacacagcc agtctgcacg tcgcatagtg actggatatg
     3601 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     3661 atttatatca ttttacgttt ctcgttcaac tttttaatac aaagttgcct ccggaacata
     3721 atggtgcatc tagatgccca ttcctgtaga cttcttaatt aagacgtcag aattctcgag
     3781 gcggccgcat gtgagtctcc ctatagtgag tcgtattaat ttcgcgggcg cgtcagcggg
     3841 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     3901 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     3961 gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg
     4021 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     4081 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     4141 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     4201 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     4261 gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     4321 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     4381 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     4441 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     4501 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     4561 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     4621 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     4681 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     4741 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     4801 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     4861 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     4921 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     4981 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     5041 caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg
     5101 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     5161 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     5221 agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     5461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6061 gagcgaggaa atcgtcacct gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt
     6121 gtgtatcgct cgagggatcc gaattcgaag tcttggtacg gaggggatcc gatcgacaag
     6181 ccgaacatat gggttttttg ctcacctgtg attgctccta ctcaaataca aaaacatcaa
     6241 attttctgtc aataaagcat atttatttat atttatttta caggaaagaa ttccttttaa
     6301 agtgtatttt aacctataat gaaaaacgat taaaaaaaat acataaaata attcgaaaat
     6361 ttttgaatag cccaggttga taaaaattca tttcatacgt tttataactt atgcccctaa
     6421 gtattttttg accatagtgt ttcaattcta cattaatttt acagagtaga atgaaacgcc
     6481 acctactcag ccaagaggcg aaaaggttag ctcgccaagc agagagggcg ccagtgctca
     6541 ctacttttta taattctcaa cttctttttc cagactcagt tcgtatatat agacctattt
     6601 tcaatttaac gtcggggcct caactttgta taataaagtt gaacgagaaa cgtaaaatga
     6661 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     6721 aacacaacat atccagtcac tatgggccgc attaggcacc ccaggcttta cactttatgc
     6781 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctac
     6841 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6901 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6961 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7021 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7081 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7141 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7201 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7261 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7321 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7381 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7441 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7501 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7561 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7621 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtcccgcggt atcattgcag
     7681 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7741 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7801 ggtaatggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt
     7861 tgcggtataa gaatatatac tgatatgtat ccgaagtatg tcaaaaagag gtatgctatg
     7921 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
     7981 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
     8041 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
     8101 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc
     8161 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tatcgataca
     8221 ccgggccgtc gcatggttat tccacttgcg tctgcgcgct tgctctcaga taaagtctcc
     8281 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     8341 atggccagtg tgccggtttc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     8401 aatgacatca aaaacgccat taacctgatg ttctggggaa tataatgtca ggctccctta
     8461 tacacagcca gtctgcacgt cgcatagtga ctggatatgt tgtgttttac agtattatgt
     8521 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     8581 tcgttcaact tttctataca aagttgtcca gctggagcga gaccggagat actcgaattc
     8641 gttgtcggct catatacatg ggcgaaataa tttcgaatat gttttaaaaa taaccaaaga
     8701 cattagaaag agatcgccaa tacttataca ttatgtctat gtgtgccatg tggtagcatg
     8761 agccaaaaag cttctcgaaa ttacgaatta catatagacg aatatgtatg tgactttagt
     8821 ttcgaaataa tttcgagaat tttaaaaata acgcattcgt taaaagttcg cgtcaattcg
     8881 aatcgaattt tcgattgtcg atttagtgtg gattgtcgaa aatcgttccg ccttcgaagt
     8941 ttactgaaag gaatcattgc gatctcgtga attgcttgta tgagaacacg ccaccatatc
     9001 gagatacctg ttcaaaatat cgaaatcgcg ttccaaacgg tagtagttac cagttgagtg
     9061 agttgtggtg acagtttaac ctctctacat ttgtaataat aagcattgtt ttggcgtatc
     9121 aatagtctgt agattttttc acaaagaaac ctggagaaat gtacacaaat atctaaatga
     9181 aaattggcgt tacgtaacaa ataccaacgc cccgaaaatc cgtatatatg caaagcattc
     9241 tgatatatat atatataagt ggtatacata tacatattcg tatcggatcc gtgatctata
     9301 tttaggaacc ctcgaccgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt
     9361 caattcaaac aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc
     9421 agctgaacaa gctaaacaat cggggtaccg ctagagtcga cggtaccgcg ggcccgggat
     9481 ccaccggtcg ccacatgagc aagggcgagg agctgttcac cggcgtggtg cccatcctgg
     9541 tggagctgga tggcgatgtg aacggccaca agttcagcgt gcgcggcgag ggcgagggcg
     9601 atgccaccat cggcaagctg
//
