LOCUS       sequence-405852-        9868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating targeting inversions. N-terminal split GFP, expressed
            in abdomen and mouthparts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT21
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    637..758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(674..693)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     728..747
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    790..913
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(936..941,942..959,960..966)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1020..1679
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1086..1105)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        1688..2133
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(2134..2220,2221..3555)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(3594..3717)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3809..3828)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3810..3828)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4281..5072
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5318..5906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5807..5826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6060..6077
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        6109..6127
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6109..6126
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        6204..6624
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    6631..6754
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6777..6782,6783..6800,6801..6807)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        6850..6954
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6955..7023,7024..7815)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7173..7192)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     8336..8355
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8495..8618)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        8670..9152
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        9156..9747
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
ORIGIN
        1 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
       61 cctggtgacc accctgacct acggcgtgca gtgcttcagc cgctactccg atcacatgaa
      121 gcgccacgat ttcttcaaga gcgccatgcc cgagggctac gtgcaggagc gcaccatcag
      181 cttcaaggat gatggcaagt acaagacccg cgccgtggtg aagttcgagg gcgataccct
      241 ggtgaaccgc atcgggctga agggcaccga tttcaaggag gatggcaaca tcctgggcca
      301 caagctggag tacaacttca acagccacaa cgtgtacatc accgccgata agcagaagaa
      361 cggcatcaag gccaacttca ccgtgcgcca caacgtggag gatggcagcg tgcagctggc
      421 cgatcactac cagcagaaca cccccatcgg cgatggcccc gtgctgctgc ccgataacca
      481 ctacctgagc acccagaccg tgctgagcaa ggatcccaac gagaagtaaa gatcataatc
      541 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
      601 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
      661 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
      721 tctagttgtg gtttgtccaa actcatcaat gtatcttaaa gcttatcgat acgcccatgg
      781 tctcaccatc aactttgtat acaaaagttg aacgagaaac gtaaaatgat ataaatatca
      841 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
      901 tccagtcact atgggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
      961 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     1021 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     1081 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     1141 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     1201 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     1261 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     1321 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     1381 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     1441 atatgttttt cgtgtcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     1501 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     1561 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     1621 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     1681 cgcgtggcac atatacctgc cgttcactat tatttagtga aatgagatat tatgatattt
     1741 tctgaattgt gattaaaaag gcaactttat gcccatgcaa cagaaactat aaaaaataca
     1801 gagaatgaaa agaaacagat agatttttta gttctttagg cccgtagtct gcaaatcctt
     1861 ttatgatttt ctatcaaaca aaagaggaaa atagaccagt tgcaatccaa acgagagtct
     1921 aatagaatga ggtcgaaaag taaatcgcgc gggtttgtta ctgataaagc aggcaagacc
     1981 taaaatgtgt aaagggcaaa gtgtatactt tggcgtcacc ccttacatat tttaggtctt
     2041 tttttattgt gcgtaactaa cttgccatct tcaaacagga gggctggaag aagcagaccg
     2101 ctaacacagt acataaaaaa ggagacatga acgatgaaca tcaaaaagtt tgcaaaacaa
     2161 gcaacagtat taacctttac taccgcactg ctggcaggag gcgcaactca agcgtttgcg
     2221 aaagaaacga accaaaagcc atataaggaa acatacggca tttcccatat tacacgccat
     2281 gatatgctgc aaatccctga acagcaaaaa aatgaaaaat atcaagttcc tgaattcgat
     2341 tcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg acgtttggga cagctggcca
     2401 ttacaaaacg ctgacggcac tgtcgcaaac tatcacggct accacatcgt ctttgcatta
     2461 gccggagatc ctaaaaatgc ggatgacaca tcgatttaca tgttctatca aaaagtcggc
     2521 gaaacttcta ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag cgacaaattc
     2581 gatgcaaatg attctatcct aaaagaccaa acacaagaat ggtcaggttc agccacattt
     2641 acatctgacg gaaaaatccg tttattctac actgatttct ccggtaaaca ttacggcaaa
     2701 caaacactga caactgcaca agttaacgta tcagcatcag acagctcttt gaacatcaac
     2761 ggtgtagagg attataaatc aatctttgac ggtgacggaa aaacgtatca aaatgtacag
     2821 cagttcatcg atgaaggcaa ctacagctca ggcgacaacc atacgctgag agatcctcac
     2881 tacgtagaag ataaaggcca caaatactta gtatttgaag caaacactgg aactgaagat
     2941 ggctaccaag gcgaagaatc tttatttaac aaagcatact atggcaaaag cacatcattc
     3001 ttccgtcaag aaagtcaaaa acttctgcaa agcgataaaa aacgcacggc tgagttagca
     3061 aacggcgctc tcggtatgat tgagctaaac gatgattaca cactgaaaaa agtgatgaaa
     3121 ccgctgattg catctaacac agtaacagat gaaattgaac gcgcgaacgt ctttaaaatg
     3181 aacggcaaat ggtacctgtt cactgactcc cgcggatcaa aaatgacgat tgacggcatt
     3241 acgtctaacg atatttacat gcttggttat gtttctaatt ctttaactgg cccatacaag
     3301 ccgctgaaca aaactggcct tgtgttaaaa atggatcttg atcctaacga tgtaaccttt
     3361 acttactcac acttcgctgt acctcaagcg aaaggaaaca atgtcgtgat tacaagctat
     3421 atgacaaaca gaggattcta cgcagacaaa caatcaacgt ttgcgccaag cttcctgctg
     3481 aacatcaaag gcaagaaaac atctgttgtc aaagacagca tccttgaaca aggacaatta
     3541 acagttaaca aataaatgtc aggctccctt atacacagcc agtctgcacg tcgcatagtg
     3601 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
     3661 atatattgat atttatatca ttttacgttt ctcgttcaac tttttaatac aaagttgcct
     3721 ccggaacata atggtgcatc tagatgccca ttcctgtaga cttcttaatt aagacgtcag
     3781 aattctcgag gcggccgcat gtgagtctcc ctatagtgag tcgtattaat ttcgcgggcg
     3841 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     3901 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     3961 tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga
     4021 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     4081 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     4141 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     4201 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     4261 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     4321 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     4381 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     4441 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     4501 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     4561 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     4621 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     4681 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     4741 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     4801 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     4861 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     4921 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     4981 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     5041 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     5101 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     5161 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac
     5221 acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact
     5281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5461 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5941 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6001 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6061 gcgagtcagt gagcgaggaa atcgtcacct gcaggaaggt ttaaacgcat ttaggtgaca
     6121 ctatagaagt gtgtatcgct cgagggatcc gaattcgaag tcttggtacg gaggggatcc
     6181 gatcgacaag ccgaacatat gggttttttg ctcacctgtg attgctccta ctcaaataca
     6241 aaaacatcaa attttctgtc aataaagcat atttatttat atttatttta caggaaagaa
     6301 ttccttttaa agtgtatttt aacctataat gaaaaacgat taaaaaaaat acataaaata
     6361 attcgaaaat ttttgaatag cccaggttga taaaaattca tttcatacgt tttataactt
     6421 atgcccctaa gtattttttg accatagtgt ttcaattcta cattaatttt acagagtaga
     6481 atgaaacgcc acctactcag ccaagaggcg aaaaggttag ctcgccaagc agagagggcg
     6541 ccagtgctca ctacttttta taattctcaa cttctttttc cagactcagt tcgtatatat
     6601 agacctattt tcaatttaac gtcggggcct caactttgta taataaagtt gaacgagaaa
     6661 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     6721 aatactgtaa aacacaacat atccagtcac tatgggccgc attaggcacc ccaggcttta
     6781 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt
     6841 caggagctac gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6901 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6961 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7021 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7081 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7141 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7201 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7261 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7321 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7381 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7441 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7501 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7561 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7621 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtcccgcggt
     7681 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7741 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7801 attaagcatt ggtaatggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     7861 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     7921 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     7981 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     8041 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     8101 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     8161 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     8221 attatcgata caccgggccg tcgcatggtt attccacttg cgtctgcgcg cttgctctca
     8281 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     8341 atgaccaccg atatggccag tgtgccggtt tccgttatcg gggaagaagt ggctgatctc
     8401 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataatgt
     8461 caggctccct tatacacagc cagtctgcac gtcgcatagt gactggatat gttgtgtttt
     8521 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     8581 attttacgtt tctcgttcaa cttttctata caaagttgtc cagctggagc gagaccggag
     8641 actagttgtt cgccgagctc ttacgcgccc gcgtaaaaca caatcaagta tgagtcataa
     8701 gctgatgtca tgttttgcac acggctcata accgaactgg ctttacgagt agaattctac
     8761 ttgtaacgca cgatcagtgg atgatgtcat ttgtttttca aatcgagatg atgtcatgtt
     8821 ttgcacacgg ctcataaact cgctttacga gtagaattct acgtgtaacg cacgatcgat
     8881 tgatgagtca tttgttttgc aatatgatat catacaatat gactcatttg tttttcaaaa
     8941 ccgaacttga tttacgggta gaattctact tgtaaagcac aatcaaaaag atgatgtcat
     9001 ttgtttttca aaactgaact cgctttacga gtagaattct acgtgtaaaa cacaatcaag
     9061 aaatgatgtc atttgttata aaaataaaag ctgatgtcat gttttgcaca tggctcataa
     9121 ctaaactcgc tttacgggta gaattctacg cgcgtcgatg tctttgtgat gcgcgcgaca
     9181 tttttgtagg ttattgataa aatgaacgga tacgttgccc gacattatca ttaaatcctt
     9241 ggcgtagaat ttgtcgggtc cattgtccgt gtgcgctagc atgcccgtaa cggacctcgt
     9301 acttttggct tcaaaggttt tgcgcacaga caaaatgtgc cacacttgca gctctgcatg
     9361 tgtgcgcgtt accacaaatc ccaacggcgc agtgtacttg ttgtatgcaa ataaatctcg
     9421 ataaaggcgc ggcgcgcgaa tgcagctgat cacgtacgct cctcgtgttc cgttcaagga
     9481 cggtgttatc gacctcagat taatgtttat cggccgactg ttttcgtatc cgctcaccaa
     9541 acgcgttttt gcattaacat tgtatgtcgg cggatgttct atatctaatt tgaataaata
     9601 aacgataacc gcgttggttt tagagggcat aataaaagaa atattgttat cgtgttcgcc
     9661 attagggcag tataaattga cgttcatgtt ggatattgtt tcagttgcaa gttgacactg
     9721 gcggcgacaa gatcgtgaac aaccaagtga ccatgagcaa gggcgaggag ctgttcaccg
     9781 gcgtggtgcc catcctggtg gagctggatg gcgatgtgaa cggccacaag ttcagcgtgc
     9841 gcggcgaggg cgagggcgat gccaccat
//
