LOCUS       sequence-405857-        9512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating targeting inversions. C-terminal split GFP, expressed
            in eyes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT23
FEATURES             Location/Qualifiers
     source          1..9512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             128..919
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1165..1753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1654..1673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1907..1924
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1956..1974
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1956..1973
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        2051..2471
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    2478..2601
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2624..2629,2630..2647,2648..2654)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        2697..2801
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2802..2870,2871..3662)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3020..3039)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4183..4202
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4342..4465)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5838..5885
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     polyA_signal    5996..6117
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6033..6052)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6087..6106
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6149..6272
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6295..6300,6301..6318,6319..6325)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6379..7038
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6445..6464)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        7047..7492
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(7493..7579,7580..8914)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(8953..9076)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9168..9187)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9169..9187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
       61 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
      121 aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
      181 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
      241 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
      301 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
      361 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
      421 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
      481 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
      541 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
      601 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
      661 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
      721 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
      781 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
      841 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
      901 caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg
      961 cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca
     1021 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga
     1081 tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1741 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1801 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1921 cgaggaaatc gtcacctgca ggaaggttta aacgcattta ggtgacacta tagaagtgtg
     1981 tatcgctcga gggatccgaa ttcgaagtct tggtacggag gggatccgat cgacaagccg
     2041 aacatatggg ttttttgctc acctgtgatt gctcctactc aaatacaaaa acatcaaatt
     2101 ttctgtcaat aaagcatatt tatttatatt tattttacag gaaagaattc cttttaaagt
     2161 gtattttaac ctataatgaa aaacgattaa aaaaaataca taaaataatt cgaaaatttt
     2221 tgaatagccc aggttgataa aaattcattt catacgtttt ataacttatg cccctaagta
     2281 ttttttgacc atagtgtttc aattctacat taattttaca gagtagaatg aaacgccacc
     2341 tactcagcca agaggcgaaa aggttagctc gccaagcaga gagggcgcca gtgctcacta
     2401 ctttttataa ttctcaactt ctttttccag actcagttcg tatatataga cctattttca
     2461 atttaacgtc ggggcctcaa ctttgtataa taaagttgaa cgagaaacgt aaaatgatat
     2521 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2581 acaacatatc cagtcactat gggccgcatt aggcacccca ggctttacac tttatgcttc
     2641 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctacgcg
     2701 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2761 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2821 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2881 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2941 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3001 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     3061 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     3121 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     3181 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     3241 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     3301 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     3361 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     3421 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     3481 ggtttattgc tgataaatct ggagccggtg agcgtgggtc ccgcggtatc attgcagcac
     3541 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     3601 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3661 aatggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
     3721 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga
     3781 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
     3841 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
     3901 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
     3961 gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct
     4021 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt atcgatacac
     4081 cgggccgtcg catggttatt ccacttgcgt ctgcgcgctt gctctcagat aaagtctccc
     4141 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
     4201 tggccagtgt gccggtttcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
     4261 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataatgtcag gctcccttat
     4321 acacagccag tctgcacgtc gcatagtgac tggatatgtt gtgttttaca gtattatgta
     4381 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
     4441 cgttcaactt ttctatacaa agttgtccag ctggagcgag accggagcaa tggttaattc
     4501 gagctcgccc ggggatctaa ttcaattaga gactaattca attagagcta attcaattag
     4561 gatccaagct tatcgatttc gaaccctcga ccgccggagt ataaatagag gcgcttcgtc
     4621 tacggagcga caattcaatt caaacaagca aagtgaacac gtcgctaagc gaaagctaag
     4681 caaataaaca agcgcagctg aacaagctaa acaatcggat gaaaattgaa gagggcaagt
     4741 tggtcatctg gattaatgga gataagggct ataatggact ggcagaggtt ggaaaaaaat
     4801 ttgagaagga tacaggcatt aaggtcacag ttgagcatcc agataagttg gaggagaagt
     4861 tcccgcaggt ggctgcgacg ggtgacggac ccgacatcat attctgggct catgaccgtt
     4921 tcggaggata cgcccagtcc ggcttgttgg cggaaattac ccctgataaa gcttttcagg
     4981 acaaactcta ccccttcacc tgggatgctg tccgatacaa cggcaagctc attgcatacc
     5041 ccattgcagt tgaagcactg agtctgattt acaataaaga cctgttgccg aatcctccga
     5101 agacctggga ggagattccc gctctggaca aggagctgaa ggctaagggc aagtccgccc
     5161 tgatgttcaa cctgcaagag ccgtacttta cttggcccct gatcgcagcc gacggcggat
     5221 acgccttcaa atacgagaac ggaaagtacg atataaagga tgtcggagtc gacaacgccg
     5281 gcgctaaagc aggcctgact ttcctggtgg acctcataaa gaacaagcat atgaatgccg
     5341 acaccgatta cagtatagcg gaagccgcgt tcaacaaggg cgagactgcc atgacaataa
     5401 acggaccctg ggcatggtcc aacattgaca cgagcaaggt gaactacggt gtaacggtgt
     5461 tgcctacgtt caagggacag ccgagtaaac cattcgttgg cgttttgtcg gctggaatta
     5521 atgctgctag tcccaataag gagttggcta aggagttctt ggagaactac ttgctgacag
     5581 acgagggctt ggaggcagta aacaaggata agcccctggg tgcggttgcc ctcaagtcct
     5641 atgaagaaga gctcgtaaaa gacccgcgta ttgctgcgac catggagaat gcccagaagg
     5701 gtgagattat gcccaatata ccccagatgt ccgcgttttg gtatgcagtt cgcaccgcgg
     5761 ttataaacgc agcctcggga cggcagaccg tggatgagga cttgaaagac gcccagacac
     5821 gcataaccaa acagtttcgc gaccatatgg ttctgcatga atatgtcaac gcagcaggca
     5881 ttacttaaag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     5941 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     6001 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6061 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaaag
     6121 cttatcgata cgcccatggt ctcaccatca actttgtata caaaagttga acgagaaacg
     6181 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     6241 tactgtaaaa cacaacatat ccagtcacta tgggccgcat taggcacccc aggctttaca
     6301 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
     6361 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     6421 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     6481 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     6541 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     6601 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     6661 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     6721 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     6781 cctaaagggt ttattgagaa tatgtttttc gtgtcagcca atccctgggt gagtttcacc
     6841 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     6901 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     6961 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     7021 tggcagggcg gggcgtaaac gcgtggcaca tatacctgcc gttcactatt atttagtgaa
     7081 atgagatatt atgatatttt ctgaattgtg attaaaaagg caactttatg cccatgcaac
     7141 agaaactata aaaaatacag agaatgaaaa gaaacagata gattttttag ttctttaggc
     7201 ccgtagtctg caaatccttt tatgattttc tatcaaacaa aagaggaaaa tagaccagtt
     7261 gcaatccaaa cgagagtcta atagaatgag gtcgaaaagt aaatcgcgcg ggtttgttac
     7321 tgataaagca ggcaagacct aaaatgtgta aagggcaaag tgtatacttt ggcgtcaccc
     7381 cttacatatt ttaggtcttt ttttattgtg cgtaactaac ttgccatctt caaacaggag
     7441 ggctggaaga agcagaccgc taacacagta cataaaaaag gagacatgaa cgatgaacat
     7501 caaaaagttt gcaaaacaag caacagtatt aacctttact accgcactgc tggcaggagg
     7561 cgcaactcaa gcgtttgcga aagaaacgaa ccaaaagcca tataaggaaa catacggcat
     7621 ttcccatatt acacgccatg atatgctgca aatccctgaa cagcaaaaaa atgaaaaata
     7681 tcaagttcct gaattcgatt cgtccacaat taaaaatatc tcttctgcaa aaggcctgga
     7741 cgtttgggac agctggccat tacaaaacgc tgacggcact gtcgcaaact atcacggcta
     7801 ccacatcgtc tttgcattag ccggagatcc taaaaatgcg gatgacacat cgatttacat
     7861 gttctatcaa aaagtcggcg aaacttctat tgacagctgg aaaaacgctg gccgcgtctt
     7921 taaagacagc gacaaattcg atgcaaatga ttctatccta aaagaccaaa cacaagaatg
     7981 gtcaggttca gccacattta catctgacgg aaaaatccgt ttattctaca ctgatttctc
     8041 cggtaaacat tacggcaaac aaacactgac aactgcacaa gttaacgtat cagcatcaga
     8101 cagctctttg aacatcaacg gtgtagagga ttataaatca atctttgacg gtgacggaaa
     8161 aacgtatcaa aatgtacagc agttcatcga tgaaggcaac tacagctcag gcgacaacca
     8221 tacgctgaga gatcctcact acgtagaaga taaaggccac aaatacttag tatttgaagc
     8281 aaacactgga actgaagatg gctaccaagg cgaagaatct ttatttaaca aagcatacta
     8341 tggcaaaagc acatcattct tccgtcaaga aagtcaaaaa cttctgcaaa gcgataaaaa
     8401 acgcacggct gagttagcaa acggcgctct cggtatgatt gagctaaacg atgattacac
     8461 actgaaaaaa gtgatgaaac cgctgattgc atctaacaca gtaacagatg aaattgaacg
     8521 cgcgaacgtc tttaaaatga acggcaaatg gtacctgttc actgactccc gcggatcaaa
     8581 aatgacgatt gacggcatta cgtctaacga tatttacatg cttggttatg tttctaattc
     8641 tttaactggc ccatacaagc cgctgaacaa aactggcctt gtgttaaaaa tggatcttga
     8701 tcctaacgat gtaaccttta cttactcaca cttcgctgta cctcaagcga aaggaaacaa
     8761 tgtcgtgatt acaagctata tgacaaacag aggattctac gcagacaaac aatcaacgtt
     8821 tgcgccaagc ttcctgctga acatcaaagg caagaaaaca tctgttgtca aagacagcat
     8881 ccttgaacaa ggacaattaa cagttaacaa ataaatgtca ggctccctta tacacagcca
     8941 gtctgcacgt cgcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     9001 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact
     9061 ttttaataca aagttgcctc cggaacataa tggtgcatct agatgcccat tcctgtagac
     9121 ttcttaatta agacgtcaga attctcgagg cggccgcatg tgagtctccc tatagtgagt
     9181 cgtattaatt tcgcgggcgc gtcagcggga gagccgttgt aaggcggcag actttgctca
     9241 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     9301 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt
     9361 cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     9421 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     9481 gcggttttca tggcttgtta tgactgtttt tt
//
