LOCUS       sequence-405863-       11611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3x HA-VPS4A with E228Q dominant negative mutation in Dox-inducible 
            pInducer20 vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Qualls-Histed SJ, Nielsen CP, MacGurn JA
  TITLE     Lysosomal trafficking of the glucose transporter GLUT1 requires 
            sequential regulation by TXNIP and ubiquitin.
  JOURNAL   iScience. 2023 Feb 6;26(3):106150. doi: 10.1016/j.isci.2023.106150. 
            eCollection 2023 Mar 17.
  PUBMED    36890792
REFERENCE   2  (bases 1 to 11611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-02-6"; volume: "26(3):106150. doi"; pages: " 
            10.1016/j.isci.2023.106150. eCollection 2023 Mar 17"
COMMENT     Sequence Label: E228Q Vps4A-HA
FEATURES             Location/Qualifiers
     source          1..11611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2765..2854
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    complement(4198..4222)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4275..4301
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4387..5598
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5435..5454
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5657..6274,6275..6280,6281..6316,6317..6319,6320..6355,
                     6356..6358,6359..6394,6395..6403)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6441..7014
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6608..6625)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6835..6854
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7030..7824
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7030..7824
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7084..7103)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7694..7713
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8001..8589
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8054..8074)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8472..8483)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8664..8897
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9016..9034)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9102..9206
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9207..9275,9276..10067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9425..9444)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10238..10826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10727..10746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10980..10997
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11237..11258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11273..11278,11279..11296,11297..11303)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11311..11327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11316..11338
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11335..11351
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11335..11351
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11401..11420
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatccatgtc
     2761 aacctaccca tacgatgtac cagattacgc cggttaccct tatgatgtac ctgactacgc
     2821 tggaagttac ccttacgatg ttccggatta cgctggttct atgacgactt ccactcttca
     2881 aaaggctatc gaccttgtaa ccaaagctac ggaggaagac aaagccaaga attacgaaga
     2941 ggcccttcga ctttatcaac atgccgtaga atattttctc catgccatca aatacgaagc
     3001 tcatagtgat aaggctaagg aaagtatccg ggcgaagtgt gttcaatacc tcgaccgggc
     3061 ggagaaactt aaggactact tgcggtctaa agaaaaacat ggaaaaaagc ctgtaaaaga
     3121 gaaccaaagt gaaggcaaag ggtctgattc agattccgaa ggggacaatc ccgagaaaaa
     3181 aaaactgcag gagcaactta tgggggccgt cgtgatggaa aagccaaaca tcaggtggaa
     3241 tgatgtggca ggtcttgagg gcgcgaaaga ggccctgaaa gaagcagtaa ttttgccaat
     3301 caagttccca catttgttta ctgggaaaag gacaccgtgg aggggaatat tgttgttcgg
     3361 gccgccaggg acgggcaaat cctacttggc gaaggctgtt gccactgaag ctaataactc
     3421 cacttttttt tcagtttctt cctctgactt gatgagcaag tggctcggag agtcagaaaa
     3481 actcgtcaaa aacttgttcg agcttgcgcg ccagcataag ccgagtatca tattcatcga
     3541 tcaagtagat agtttgtgcg gctctcgcaa cgagaatgag tctgaggccg cacgccgaat
     3601 taagacagaa tttctcgtcc aaatgcaggg cgttggaaac aataacgacg ggacgttggt
     3661 ccttggggcc actaacattc cttgggtact tgatagcgcc attaggagaa gattcgaaaa
     3721 gcggatttac ataccactgc ctgaggaggc agctcgagcc caaatgttta ggctgcacct
     3781 tggcagtact cctcacaacc tgacggatgc gaacatacac gagcttgcta ggaagaccga
     3841 gggctactca ggcgctgata tatctataat tgtaagagat tcactgatgc aacccgtgag
     3901 gaaagttcaa tccgccactc actttaaaaa ggtctgtggt ccctcacgaa ccaacccgtc
     3961 aatgatgata gacgacctcc tgactccttg ctctcccgga gatccagggg ctatggagat
     4021 gacatggatg gatgtgcccg gcgataaact gttggagccc gtcgtatgta tgtccgacat
     4081 gttgcgctcc ttggcaacca caagaccaac cgtcaatgct gacgacctgc tgaaggtgaa
     4141 aaagtttagt gaggacttcg gccaagagtc ttgagcggcc gcactcgaga tatctagacc
     4201 cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg
     4261 gcccgcggtt cgaataccca tacgacgtcc cagactacgc ttagtaatga ttaattaaac
     4321 tagaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagt aacccaccca
     4381 agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga
     4441 gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca
     4501 ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca
     4561 ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag
     4621 gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac
     4681 gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat
     4741 ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc
     4801 tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga
     4861 gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg
     4921 ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg
     4981 ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga
     5041 ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg
     5101 gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc
     5161 ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt
     5221 cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta
     5281 ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc
     5341 ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt
     5401 tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc
     5461 tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt
     5521 gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt
     5581 ttggcttttt tgttagacga agcttggtac cgagctcgga tctccacccc gtaccggtcc
     5641 tgcagtcgaa ttcaccatgt ctagactgga caagagcaaa gtcataaacg gagctctgga
     5701 attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg
     5761 agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct
     5821 gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg
     5881 gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga
     5941 cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa
     6001 tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc
     6061 cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa
     6121 agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga
     6181 gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg
     6241 tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt
     6301 tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc
     6361 tgacgctctt gacgattttg accttgacat gctccccggg taactaagta aggatccgcg
     6421 gccgcactag aggaattccg cccctctccc cccccccccc ctaacgttac tggccgaagc
     6481 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     6541 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     6601 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     6661 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     6721 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     6781 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     6841 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     6901 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     6961 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttac
     7021 cggtccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     7081 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     7141 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     7201 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     7261 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     7321 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     7381 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     7441 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     7501 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
     7561 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     7621 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     7681 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     7741 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     7801 cgccttcttg acgagttctt ctgatgtaca agtaaagcgg ccgcgactct agatcataat
     7861 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     7921 gaacctgaaa cataaaatga atgcaattgt tgttgtttag tccctcccaa ttcgatatca
     7981 agcttatcgc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg
     8041 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     8101 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     8161 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     8221 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     8281 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     8341 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     8401 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     8461 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     8521 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     8581 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca
     8641 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     8701 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8761 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8821 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8881 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     8941 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt
     9001 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     9061 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9121 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     9181 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     9241 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     9301 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     9361 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     9421 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     9481 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9541 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9601 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9661 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9721 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9781 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9841 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9901 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9961 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    10021 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    10081 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    10141 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    10201 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    10261 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    10321 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10381 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10441 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10501 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10561 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10621 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    10681 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10741 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10801 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10861 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10921 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10981 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    11041 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
    11101 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    11161 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
    11221 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    11281 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    11341 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
    11401 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    11461 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    11521 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    11581 tttgttaaat atgtactaca aacttagtag t
//
