LOCUS       sequence-405864-       11380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible human TXNIP with py motif mutations (ppcy,ppty 
            -->paca,pata) in pInducer20 vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11380)
  AUTHORS   Qualls-Histed SJ, Nielsen CP, MacGurn JA
  TITLE     Lysosomal trafficking of the glucose transporter GLUT1 requires 
            sequential regulation by TXNIP and ubiquitin.
  JOURNAL   iScience. 2023 Feb 6;26(3):106150. doi: 10.1016/j.isci.2023.106150. 
            eCollection 2023 Mar 17.
  PUBMED    36890792
REFERENCE   2  (bases 1 to 11380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-02-6"; volume: "26(3):106150. doi"; pages: " 
            10.1016/j.isci.2023.106150. eCollection 2023 Mar 17"
COMMENT     Sequence Label: TXNIP-py
FEATURES             Location/Qualifiers
     source          1..11380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3967..3991)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4044..4070
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4156..5367
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5204..5223
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5426..6043,6044..6049,6050..6085,6086..6088,6089..6124,
                     6125..6127,6128..6163,6164..6172)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6210..6783
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6377..6394)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6604..6623
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6799..7593
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6799..7593
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6853..6872)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7463..7482
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7770..8358
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7823..7843)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8241..8252)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8433..8666
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8785..8803)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8871..8975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8976..9044,9045..9836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9194..9213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10007..10595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10496..10515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10749..10766
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11006..11027
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11042..11047,11048..11065,11066..11072)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11080..11096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11085..11107
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11104..11120
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11104..11120
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11170..11189
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatccggtac
     2761 cgaattcgcg gccgcaatgg tgatgttcaa gaagatcaag tcttttgagg tggtctttaa
     2821 cgaccctgaa aaggtgtacg gcagtggcga gaaggtggct ggccgggtga tagtggaggt
     2881 gtgtgaagtt actcgtgtca aagccgttag gatcctggct tgcggagtgg ctaaagtgct
     2941 ttggatgcag ggatcccagc agtgcaaaca gacttcggag tacctgcgct atgaagacac
     3001 gcttcttctg gaagaccagc caacaggtga gaatgagatg gtgatcatga gacctggaaa
     3061 caaatatgag tacaagttcg gctttgagct tcctcagggg cctctgggaa catccttcaa
     3121 aggaaaatat gggtgtgtag actactgggt gaaggctttt cttgaccgcc cgagccagcc
     3181 aactcaagag acaaagaaaa actttgaagt agtggatctg gtggatgtca atacccctga
     3241 tttaatggca cctgtgtctg ctaaaaaaga aaagaaagtt tcctgcatgt tcattcctga
     3301 tgggcgggtg tctgtctctg ctcgaattga cagaaaagga ttctgtgaag gtgatgagat
     3361 ttccatccat gctgactttg agaatacatg ttcccgaatt gtggtcccca aagctgccat
     3421 tgtggcccgc cacacttacc ttgccaatgg ccagaccaag gtgctgactc agaagttgtc
     3481 atcagtcaga ggcaatcata ttatctcagg gacatgcgca tcatggcgtg gcaagagcct
     3541 tcgggttcag aagatcaggc cttctatcct gggctgcaac atccttcgag ttgaatattc
     3601 cttactgatc tatgttagcg ttcctggatc caagaaggtc atccttgacc tgcccctggt
     3661 aattggcagc agatcaggtc taagcagcag aacatccagc atggccagcc gaaccagctc
     3721 tgagatgagt tgggtagatc tgaacatccc tgatacccca gaagctcctg cctgcgccat
     3781 ggatgtcatt cctgaagatc accgattgga gagcccaacc actcctctgc tagatgacat
     3841 ggatggctct caagacagcc ctatctttat gtatgcccct gagttcaagt tcatgccacc
     3901 agccactgcc actgaggtgg atccctgcat cctcaacaac aatgtgcagt gactcgagat
     3961 atctagaccc agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga
     4021 gtctagaggg cccgcggttc gaatacccat acgacgtccc agactacgct tagtaatgat
     4081 taattaaact agaaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagta
     4141 acccacccaa gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc
     4201 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
     4261 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
     4321 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
     4381 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
     4441 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
     4501 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
     4561 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
     4621 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
     4681 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
     4741 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
     4801 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
     4861 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
     4921 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     4981 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     5041 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     5101 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     5161 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     5221 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     5281 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     5341 tggccgtttt tggctttttt gttagacgaa gcttggtacc gagctcggat ctccaccccg
     5401 taccggtcct gcagtcgaat tcaccatgtc tagactggac aagagcaaag tcataaacgg
     5461 agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca
     5521 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     5581 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     5641 cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc
     5701 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     5761 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     5821 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     5881 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     5941 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     6001 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct
     6061 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
     6121 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa
     6181 ggatccgcgg ccgcactaga ggaattccgc ccctctcccc cccccccccc taacgttact
     6241 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     6301 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     6361 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     6421 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     6481 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     6541 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     6601 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     6661 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     6721 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     6781 taagcttacc ggtccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct
     6841 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     6901 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     6961 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     7021 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     7081 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     7141 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     7201 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     7261 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     7321 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     7381 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     7441 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     7501 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     7561 gccttctatc gccttcttga cgagttcttc tgatgtacaa gtaaagcggc cgcgactcta
     7621 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     7681 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgtttagt ccctcccaat
     7741 tcgatatcaa gcttatcgcg atagatccta atcaacctct ggattacaaa atttgtgaaa
     7801 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7861 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7921 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7981 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     8041 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     8101 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     8161 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     8221 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     8281 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     8341 tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga
     8401 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     8461 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     8521 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8581 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8641 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     8701 ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc
     8761 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     8821 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8881 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8941 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9001 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9061 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9121 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9181 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     9241 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9301 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9361 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9421 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9481 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     9541 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     9601 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9661 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9721 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9781 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9841 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9901 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9961 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10021 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10081 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10141 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10201 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10261 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10321 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10381 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10441 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10501 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10561 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10621 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10681 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    10741 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    10801 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat
    10861 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    10921 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga
    10981 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    11041 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    11101 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc
    11161 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    11221 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    11281 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    11341 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
