LOCUS       sequence-405881-        2768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cry2(1-531)-mCherry-Profilin.WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Mann N, Hill J, Wang K, Hughes RM
  TITLE     OptoProfilin: A Single Component Biosensor of Applied Cellular 
            Stress.
  JOURNAL   Chembiochem. 2024 May 2;25(9):e202400007. doi: 
            10.1002/cbic.202400007. Epub 2024 Mar 28.
  PUBMED    38457348
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem."; date: "2024-05-2"; volume: "25(9):e202400007. doi"; 
            pages: " 10.1002/cbic.202400007. Epub 2024 Mar 28"
COMMENT     Sequence Label: OptoProfilin
FEATURES             Location/Qualifiers
     source          1..2768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1494
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     regulatory      1612..1621
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1618..2325
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1763..1781)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2031..2050
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgaagatgg acaaaaagac tatagtttgg tttagaagag acctaaggat tgaggataat
       61 cctgcattag cagcagctgc tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct
      121 gaagaagaag gacagtttta tcctggaaga gcttcaagat ggtggatgaa acaatcactt
      181 gctcacttat ctcaatcctt gaaggctctt ggatctgacc tcactttaat caaaacccac
      241 aacacgattt cagcgatctt ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt
      301 aaccacctct atgatcctgt ttcgttagtt cgggaccata ccgtaaagga gaagctggtg
      361 gaacgtggga tctctgtgca aagctacaat ggagatctat tgtatgaacc gtgggagata
      421 tactgcgaaa agggcaaacc ttttacgagt ttcaattctt actggaagaa atgcttagat
      481 atgtcgattg aatccgttat gcttcctcct ccttggcggt tgatgccaat aactgcagcg
      541 gctgaagcga tttgggcgtg ttcgattgaa gaactagggc tggagaatga ggccgagaaa
      601 ccgagcaatg cgttgttaac tagagcttgg tctccaggat ggagcaatgc tgataagtta
      661 ctaaatgagt tcatcgagaa gcagttgata gattatgcaa agaacagcaa gaaagttgtt
      721 gggaattcta cttcactact ttctccgtat ctccatttcg gggaaataag cgtcagacac
      781 gttttccagt gtgcccggat gaaacaaatt atatgggcaa gagataagaa cagtgaagga
      841 gaagaaagtg cagatctttt tcttagggga atcggtttaa gagagtattc tcggtatata
      901 tgtttcaact tcccgtttac tcacgagcaa tcgttgttga gtcatcttcg gtttttccct
      961 tgggatgctg atgttgataa gttcaaggcc tggagacaag gcaggaccgg ttatccgttg
     1021 gtggatgccg gaatgagaga gctttgggct accggatgga tgcataacag aataagagtg
     1081 attgtttcaa gctttgctgt gaagtttctt ctccttccat ggaaatgggg aatgaagtat
     1141 ttctgggata cacttttgga tgctgatttg gaatgtgaca tccttggctg gcagtatatc
     1201 tctgggagta tccccgatgg ccacgagctt gatcgcttgg acaatcccgc gttacaaggc
     1261 gccaaatatg acccagaagg tgagtacata aggcaatggc ttcccgagct tgcgagattg
     1321 ccaactgaat ggatccatca tccatgggac gctcctttaa ccgtactcaa agcttctggt
     1381 gtggaactcg gaacaaacta tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta
     1441 ctagctaaag ctatttcaag aacccgtgaa gcacagatca tgatcggagc agcacctgat
     1501 gagattgtag cagatagctt cgaggcctta ggggctaata ccattaaaga acctggtctt
     1561 tgcccatctg tgtcttctaa tgaccaacaa gtagcccggg atccaccggt cgccaccatg
     1621 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     1681 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     1741 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     1801 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     1861 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     1921 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     1981 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     2041 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     2101 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     2161 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     2221 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     2281 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggcgg cagcggcggc
     2341 ggcatggccg ggtggaacgc ctacatcgac agccttatgg cggacgggac ctgtcaggac
     2401 gcggccatcg taggctacaa ggactcgccc tccgtctggg ccgccgtccc cgggaagacc
     2461 ttcgttagca ttacgccagc tgaggttggt gtcctggtag gcaaagaccg gtcaagtttt
     2521 ttcgtcaatg ggctgacact tgggggccag aaatgttctg tgatccggga ctcactgctg
     2581 caagacgggg aatttacaat ggatcttcgt accaagagca ccggaggagc ccccaccttc
     2641 aatgtcactg tcaccatgac tgccaagacg ctagtcctgc tgatgggcaa agaaggtgtc
     2701 cacggtggtt tgatcaacaa gaaatgttat gaaatggcct ctcacctgcg gcgttcccag
     2761 tactaata
//
