LOCUS       sequence-405884-       13964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expression of geneticin resistance, Nluc under PgpdA 
            promoter and luciferase under a synthetic promoter containing three 
            copies of the target sequence for gRNA1 (3xLuc)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 13964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB400
FEATURES             Location/Qualifiers
     source          1..13964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     206..225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     459..476
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    491..631
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(557..576)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     717..739
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      975..1169
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1235..2308)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(2737..3366)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4667..4691
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(4965..6617)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     6533..6551
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             10893..11687
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10947..10966)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11557..11576
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    11992..12016
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12802..13593
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(13681..13964,1..305)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
       61 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      121 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      181 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      241 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      301 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      361 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      421 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      481 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      541 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
      601 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
      661 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtcaccgg
      721 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
      781 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
      841 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
      901 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
      961 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     1021 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     1081 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     1141 ttgggttccc aatgtacgtg ctatccacag gaaagagtcc ttttcgacct ttttcccctg
     1201 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
     1261 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
     1321 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
     1381 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
     1441 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
     1501 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
     1561 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
     1621 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
     1681 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
     1741 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
     1801 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
     1861 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
     1921 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
     1981 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
     2041 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
     2101 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
     2161 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
     2221 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
     2281 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
     2341 attgcagggc cggcaggcaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
     2401 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
     2461 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
     2521 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
     2581 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
     2641 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
     2701 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
     2761 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
     2821 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
     2881 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
     2941 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
     3001 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
     3061 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
     3121 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
     3181 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
     3241 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
     3301 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
     3361 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
     3421 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
     3481 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
     3541 atttcatgca gccgcacggt tgagaggtgc gcgggcggct cgaacacgta cccggccgcg
     3601 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
     3661 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
     3721 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
     3781 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
     3841 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
     3901 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
     3961 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
     4021 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
     4081 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
     4141 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
     4201 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
     4261 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
     4321 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
     4381 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
     4441 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagatgac ggctgcactg
     4501 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc
     4561 gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg
     4621 cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat
     4681 atatcctgtc aggatccggt ctcatgacag cgcatgtctc agacggtcga tgcattgcag
     4741 atgagctgta tctggaagag gtaaacccga aacgcgtttt attcttgttg acatggagct
     4801 attaaatcac tagaaggcac tctttgctgc ttggacaaat gaacgtatct tatcgagatc
     4861 ctgaacacca tttgtctcaa ctccggagct gacatcgaca ccaacgatct tatatccaga
     4921 ttcgtcaagc tgtttgatga tttcagtaac gttaagtaag ctcattacac ggcgatcttt
     4981 ccgcccttct tggcctttat gaggatctct ctgatttttc ttgcgtcgag ttttccggta
     5041 agacctttcg gtacttcgtc cacaaacaca actcctccgc gcaacttttt cgcggttgtt
     5101 acttgactgg cgacgtaatc cacgatctct ttttccgtca tcgtctttcc gtgctccaaa
     5161 acaacaacgg cggcgggaag ttcaccggcg tcatcgtcgg gaagacctgc gacacctgcg
     5221 tcgaagatgt tggggtgttg gagcaagatg gattccaatt cagcgggagc cacctgatag
     5281 cctttgtact taatcagaga cttcaggcgg tcaacgatga agaagtgttc gtcttcgtcc
     5341 cagtaagcta tgtctccaga atgtagccat ccatccttgt caatcaaggc gttggtcgct
     5401 tccggattgt ttacataacc ggacataatc ataggacctc tcacacacag ttcgcctctt
     5461 tgattaacgc ccagcgtttt cccggtatcc agatccacaa ccttcgcttc aaaaaatgga
     5521 acaactttac cgaccgcgcc cggtttatca tccccctcgg gtgtaatcag aatagctgat
     5581 gtagtctcag tgagcccata tccttgcctg atacctggca gatggaacct cttggcaacc
     5641 gcttccccga cttccttaga gaggggagcg ccaccagaag caatttcgtg taaattagat
     5701 aaatcgtatt tgtcaatcag agtgcttttg gcgaagaagg agaatagggt tggcaccagc
     5761 agcgcacttt gaatcttgta atcctgaagg ctcctcagaa acagctcttc ttcaaatcta
     5821 tacattaaga cgactcgaaa tccacatatc aaatatccga gtgtagtaaa cattccaaaa
     5881 ccgtgatgga atggaacaac acttaaaatc gcagtatccg gaatgatttg attgccaaaa
     5941 ataggatctc tggcatgcga gaatctcacg caggcagttc tatgaggcag agcgacacct
     6001 ttaggcagac cagtagatcc agaggagttc atgatcagtg caattgtctt gtccctatcg
     6061 aaggactctg gcacaaaatc gtattcatta aaaccgggag gtagatgaga tgtgacgaac
     6121 gtgtacatcg actgaaatcc ctggtaatcc gttttagaat ccatgataat aattttttgg
     6181 atgattggga gctttttttg cacgttcaaa attttttgca accccttttt ggaaacgaac
     6241 accacggtag gctgcgaaat gcccatactg ttgagcaatt cacgttcatt ataaatgtcg
     6301 ttcgcgggcg caactgcaac tccgataaat aacgcgccca acaccggcat aaagaattga
     6361 agagagtttt cactgcatac gacgattctg tgatttgtat tcagcccata tcgtttcata
     6421 gcttctgcca accgaacgga catttcgaag tactcagcgt aagtgatgtc cacctcgata
     6481 tgtgcatctg taaaagcaat tgttccagga accagggcgt atctcttcat agccttatgc
     6541 agttgctctc cagcggttcc atcttccagc ggatagaatg gcgccgggcc tttctttatg
     6601 tttttggcgt cttccattta tgaagggctt gagatgatga tcctctggag aaggttaaag
     6661 tggttgaaga tatcgaaatg ctgtgatggg agtgatacta tatggaattg agggtcatga
     6721 agccgccttt ataaaccaga tcatgagctc tgtgtgaaag gtgacaggga gagataaccg
     6781 gggagactgg ttggtgagag aagacggacc ctcgagcgta agttctgcgg tcgcgactgg
     6841 ttggtgagag aagaaggctt ggtcgactca ggtccgaccc gggtgaaccc aactgactgg
     6901 ttggtgagag aagagggagt cattgaggac agaattttgg cagaggcaat tagtgtcata
     6961 tgatgtgaaa ttagggtctg tatcgcaata atttggtgag ttaaattgga taaggggttt
     7021 ggaagacgat ttggcacagt tagtcacagg cggaggactt gccttcaaaa ggcggttact
     7081 gttgtctgtc ttgggttaca gcgaaagcag cagtcgttga ggcaaaagtg atacgtccga
     7141 cattgcatct aggcaaggca acttcgcaac aggtcaaggt gttctttgta gaggaactag
     7201 aggtccagtt caaacactct cgtttgtgca gctcgtttat gcatacggcg tgtaaactgg
     7261 tgcgtcggtg tatggggacc ttccttcttg cgtatgatcg catggactgc gatcacgttt
     7321 tgtgggttcg ctgggactgt cacgcttaac cgctcggaat tcgagaaagc atacgactgc
     7381 tcgcaagggc gctctgatcg aacacgacca taacggggca cacacgataa tcatcacctc
     7441 tttagtccct gttacagagc agccgggtgg acgtgtgtct accgcccact gcggatctgc
     7501 atatgatcgt tttgctgtag aaataactta ataaatagga tgccggcgaa gggtctactg
     7561 tacttatacg accgggctac gtgttaacta caaggctatg tcctattgtt gacctagtgc
     7621 atacataaca tggtttcgta catgtagcgt tcaggggcta tagcaaatcc gacggtaaac
     7681 gattggggta acgccttagg ataatatgct agcctgtcgg aacacaagca cctctgccgt
     7741 gtaatccgag cgtggccacg acaatggaag gtgtggccga ccgttgttgc taatagtagg
     7801 cgatgacccg tccttctttc acaaattttc ttcagaacat tgttcacata atgtaccagg
     7861 attagccgac gtggcgagcc cgcctggttt tgggaactcg tgtttttaat tcatctccga
     7921 tcagcgcact atactgaatt ttaaagccag acatgccgat attaaaccac tcaatttgac
     7981 ctagtcctca aacgttctgc taaaccgtgt caatcagtgt ctgcttcctg agtgaaacct
     8041 ttccgccaaa gtcgacggtt agaacctagg gtcgctttcg tccttggcta caccgatctc
     8101 cttatgcagg cagtaacgta gatccattcc gtagagcggc ttttcgcttg gacatgaaac
     8161 actcctgaca gcgaaatgat tgatgaagaa caatggtgga tgaagaacaa agaaggaggg
     8221 agcttttgtt caagatgaac aaagaacaat agtggatgaa gaacaaagtg aaaaaaataa
     8281 aaaaaaatgt atggttaaat aaagagtaaa gttaccattg agactcctga cagcggggtt
     8341 tagccattaa catcgtcgtc aaagttcgat ttctggagtg tgaaaaatta gggtttcgtc
     8401 gataggagat gaaaagtcga aatttcggtg cgagaaatca aaattgatgg aagatcggcg
     8461 gcgaatttga gaggatagtg agaagaagaa gattaagaga tgatttgaat tgggagggaa
     8521 aatcgaatcg ccagtgtttg ttttagggtt tttaatctcc gggaattgaa ttttgggcgt
     8581 cgtaaaactg taaagtgtgt ttatgtagta atctttatct gagatgatac agaagagatt
     8641 ctatcccaga tacggtaaat ttgttttccc ctaggtcggt ggtcaacatt gcatttttaa
     8701 gtcaactttt aatatatttt agtttaattt ctataattta acctttttgg cattatcttt
     8761 aggaaatttt tttgacgtat tttattttac ttattaaaag tgaaaaatct taaacatatt
     8821 aattaatatc ttgcataacc aattgacatt gtaatcatca caattaaatt tgattaaaaa
     8881 tccactttct gcaaacaaac aaagtttttt ttaataaagt ctataacata tcgagtataa
     8941 tagtatcaat atagattttg atgataacaa caatagccac tcctgacgga gagcgtcatc
     9001 atgcaacatg catgtactgt ctgatgtatt aagagtatag gggtcaacaa ctagagtgtc
     9061 tgacaggtat atctagcagt ttgatttgac cattctttta ttaaaacact gtaacccgca
     9121 acgaagaact cctccgcttc aagtcaagaa tgcgcatatg tgcgtaaaat cgacaagccc
     9181 ggcggcattc caccgagcag ggtattgcct ttggcacttt acgtgcgcag aggagcctga
     9241 atgttgagtg gaatgatggt ggaatgatga agcttatgcg agaatacgtt cgcaaaggcg
     9301 ccaaccagta actccgttaa tggtaacacg aaacagcaag gagccatcag ggttgatgag
     9361 gcgctcatcg ataatcttgt ttccattcca caaagtaccg gtcacagtaa tcttcttacc
     9421 gtcgaacaca gcgataccct cgtagggtcg gccgaagtag tcaatcatgt tgggggtgac
     9481 gccatcgatc acaagagtac cgtagtgcag gataacttta aagtgatgat cgtcgactgg
     9541 ataaacgacc ttgaaaattt tctcgatctg tcccatttga tcgccagaga ggccctcgta
     9601 tgggataatg acgtggatgt caatcttaag accgttctct ccgctcaaca caatacgctg
     9661 gataggagta acggaaacgc cgagattctg gaacaaggaa gagactcctc cctgctccag
     9721 cacctggtca aggttatatc cggcggtttg gcgccagtcg ccgacgaaat cttcaagggt
     9781 gaaaaccatt ttaaaggttc ttggatggga agatgaatat actgaagatg ggaaaagaaa
     9841 gagaaaagaa aagagcagct ggtggggaga gcaggaaaat atggcaacaa atgttggact
     9901 gacgcaacga ccttgtcaac cccgccgaca caccgggcgg acagacgggg caaagctgcc
     9961 taccagggac tgagggacct cagcaggtcg agtgcagagc accggatggg tcgactgcca
    10021 gcttgtgttc ccggtctgcg ccgctggcca gctcctgagc ggcctttccg gtttcataca
    10081 ccgggcaaag caggagaggc acgatatttg gacgccctac agatgccgga tgggccaatt
    10141 agggagctta cgcgccgggt actcgctcta cctacttcgg agaaggtact atctcgtgaa
    10201 tcttttacca gatcggaagc aattggactt ctgtacctag gttaatggca tgctatttcg
    10261 ccgacggcta tacacccctg gcttcacatt ctccttcgct tactgccggt gattcgatga
    10321 agctccatat tctccgatga tgcaatagat tcttggtcaa cgaggggcac accagccttt
    10381 ccacttcggg gcggaggggc ggccggtccc ggattaataa tcatccactg cacctcagag
    10441 ccgccagagc tgtctggcgc agtggcgctt attactcagc ccttctctct gcgtccgtcc
    10501 gtctgtccgc atgcgccact ccgtcaggag aactgatatt gaaggagcat tttttgggct
    10561 tggctggagc tagtggaggt caacaatgaa tgcctatttt ggtttagtcg tccaggcggt
    10621 gagcacaaaa tttgtgtcgt ttgacaagat ggttcattta ggcaactggt cagatcagcc
    10681 ccacttgtag cagtagcggc ggcgctcgaa gtgtgactct tattagcaga caggaacgag
    10741 gacattatta tcatctgctg cttggtgcac gataacttgg tgcgtttgtc aagcaaggta
    10801 agtggacgac ccggtcatac cttcttaagt tcgcccttcc tccctttatt tcagattcaa
    10861 tctgacttac ctattctacc caagcatcca aaatgattga acaagatgga ttgcacgcag
    10921 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
    10981 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
    11041 agaccgacct gtccggtgcc ctgaatgaac tccaagacga ggcagcgcgg ctatcgtggc
    11101 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
    11161 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
    11221 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
    11281 cctgcccatt cgaccaccaa gcgaaacacc gcatcgagcg agcacgtact cggatggaag
    11341 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
    11401 tgttcgccag gctcaaggcg cggatgcccg acggcgagga tctcgtcgtg acccacggcg
    11461 acgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
    11521 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
    11581 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
    11641 attcgcagcg catagccttc tatcgccttc ttgacgagtt cttctgagct tcatcattcc
    11701 accatcattc cactcaacat tcaggctcct ctgcgcacgt aaagtgccaa aggcaatacc
    11761 ctgctcggtg gaatgccgcc gggcttgtcg attttacgca catatgcgca ttcttgactt
    11821 gaagcggagg agttcttcgt tgcgggttac agtgttttaa taaaagaatg gtcaaatcaa
    11881 actgctagat atacctgtca gacactctag ttgttgaccc ctatactctt aatacatcag
    11941 acagtacatg catgttgcat gatgacgctc tcctgagacc ggatccgtta tgtttacacc
    12001 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
    12061 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
    12121 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
    12181 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
    12241 gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
    12301 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
    12361 agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga
    12421 aaagaggaag gaaataataa gttctgttcc agccaggaca gaaatgcctc gacttcgctg
    12481 ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg
    12541 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
    12601 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
    12661 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
    12721 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
    12781 cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta
    12841 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
    12901 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
    12961 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
    13021 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
    13081 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
    13141 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
    13201 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
    13261 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
    13321 aactcgccgc ccgactgggc tggtgatgag cgaaatgtag tgcttacgtt gtcccgcatt
    13381 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
    13441 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
    13501 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
    13561 aaaggcgaga tcaccaaggt agtcggcaaa taatcaccta gaatgcatga ccaaaatccc
    13621 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    13681 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    13741 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    13801 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
    13861 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    13921 tgccagtggc gataagtcgt gtcttaccgg gttggactca agac
//
