LOCUS       sequence-405886-        9826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the expression of luciferase under a synthetic promoter 
            containing three copies of the target sequence for gRNA1 (3xLuc)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB397
FEATURES             Location/Qualifiers
     source          1..9826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1549..3201
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1615..1633)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    3470..3494
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4795..5424
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5853..6926
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6992..7186
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7422..7444)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7530..7670
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7585..7604
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7685..7702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7856..8444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7936..7955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8532..9347)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8532..9347)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9255..9274
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9780..9804
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagtgtttc atgtccaagc gaaaagccgc tctacggaat ggatctacgt tactgcctgc
       61 ataaggagat cggtgtagcc aaggacgaaa gcgaccctag gttctaaccg tcgactttgg
      121 cggaaaggtt tcactcagga agcagacact gattgacacg gtttagcaga acgtttgagg
      181 actaggtcaa attgagtggt ttaatatcgg catgtctggc tttaaaattc agtatagtgc
      241 gctgatcgga gatgaattaa aaacacgagt tcccaaaacc aggcgggctc gccacgtcgg
      301 ctaatcctgg tacattatgt gaacaatgtt ctgaagaaaa tttgtgaaag aaggacgggt
      361 catcgcctac tattagcaac aacggtcggc cacaccttcc attgtcgtgg ccacgctcgg
      421 attacacggc agaggtgctt gtgttccgac aggctagcat attatcctaa ggcgttaccc
      481 caatcgttta ccgtcggatt tgctatagcc cctgaacgct acatgtacga aaccatgtta
      541 tgtatgcact aggtcaacaa taggacatag ccttgtagtt aacacgtagc ccggtcgtat
      601 aagtacagta gacccttcgc cggcatccta tttattaagt tatttctaca gcaaaacgat
      661 catatgcaga tccgcagtgg gcggtagaca cacgtccacc cggctgctct gtaacaggga
      721 ctaaagaggt gatgattatc gtgtgtgccc cgttatggtc gtgttcgatc agagcgccct
      781 tgcgagcagt cgtatgcttt ctcgaattcc gagcggttaa gcgtgacagt cccagcgaac
      841 ccacaaaacg tgatcgcagt ccatgcgatc atacgcaaga aggaaggtcc ccatacaccg
      901 acgcaccagt ttacacgccg tatgcataaa cgagctgcac aaacgagagt gtttgaactg
      961 gacctctagt tcctctacaa agaacacctt gacctgttgc gaagttgcct tgcctagatg
     1021 caatgtcgga cgtatcactt ttgcctcaac gactgctgct ttcgctgtaa cccaagacag
     1081 acaacagtaa ccgccttttg aaggcaagtc ctccgcctgt gactaactgt gccaaatcgt
     1141 cttccaaacc ccttatccaa tttaactcac caaattattg cgatacagac cctaatttca
     1201 catcatatga cactaattgc ctctgccaaa attctgtcct caatgactcc ctcttctctc
     1261 accaaccagt cagttgggtt cacccgggtc ggacctgagt cgaccaagcc ttcttctctc
     1321 accaaccagt cgcgaccgca gaacttacgc tcgagggtcc gtcttctctc accaaccagt
     1381 ctccccggtt atctctccct gtcacctttc acacagagct catgatctgg tttataaagg
     1441 cggcttcatg accctcaatt ccatatagta tcactcccat cacagcattt cgatatcttc
     1501 aaccacttta accttctcca gaggatcatc atctcaagcc cttcataaat ggaagacgcc
     1561 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
     1621 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
     1681 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
     1741 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
     1801 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
     1861 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc
     1921 gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca
     1981 atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
     2041 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag
     2101 tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg
     2161 cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat
     2221 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
     2281 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     2341 atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt
     2401 gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac
     2461 gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg
     2521 gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag
     2581 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     2641 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     2701 aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat
     2761 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     2821 tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac
     2881 aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc
     2941 ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     3001 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     3061 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     3121 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     3181 ggcggaaaga tcgccgtgta atgagcttac ttaacgttac tgaaatcatc aaacagcttg
     3241 acgaatctgg atataagatc gttggtgtcg atgtcagctc cggagttgag acaaatggtg
     3301 ttcaggatct cgataagata cgttcatttg tccaagcagc aaagagtgcc ttctagtgat
     3361 ttaatagctc catgtcaaca agaataaaac gcgtttcggg tttacctctt ccagatacag
     3421 ctcatctgca atgcatcgac cgtctgagac atgcgcttga gacgaagctt gacaggatat
     3481 attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg
     3541 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc
     3601 gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt
     3661 cagtgcagcc gtcatctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg
     3721 ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata
     3781 cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg
     3841 gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg
     3901 cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg
     3961 agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag
     4021 accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg
     4081 gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca
     4141 tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca
     4201 cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc
     4261 tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga
     4321 aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca
     4381 gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga
     4441 ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc
     4501 gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat
     4561 cgcggccggg tacgtgttcg agccgcccgc gcacctctca accgtgcggc tgcatgaaat
     4621 cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga
     4681 aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc
     4741 ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag
     4801 gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta
     4861 gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc
     4921 agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac
     4981 cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac
     5041 ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg
     5101 attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt
     5161 aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg
     5221 atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc
     5281 attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca
     5341 accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct
     5401 gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa
     5461 acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc
     5521 tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca
     5581 agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca
     5641 tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct
     5701 aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg
     5761 tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat
     5821 ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg
     5881 tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg
     5941 cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg
     6001 atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc
     6061 cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc
     6121 gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag
     6181 ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg
     6241 ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg
     6301 aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac
     6361 acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc
     6421 tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg
     6481 ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga
     6541 tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt
     6601 accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt
     6661 tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg
     6721 cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca
     6781 agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt
     6841 tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg
     6901 agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag
     6961 caggggaaaa aggtcgaaaa ggactctttc ctgtggatag cacgtacatt gggaacccaa
     7021 agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc
     7081 ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact
     7141 ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc
     7201 gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg
     7261 ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag
     7321 gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc
     7381 aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     7441 ccggtgacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     7501 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     7561 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     7621 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     7681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7981 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8041 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8281 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8461 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
     8521 attctaggtg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     8581 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     8641 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     8701 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     8761 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
     8821 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     8881 attcgtgatt gcgcctgagc gagtcgaaat acgcgatcgc tgttaaaagg acaattacaa
     8941 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     9001 gaatcaggat attcttctaa tacctggaat gctgttttcc ctgggatcgc agtggtgagt
     9061 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     9121 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     9181 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcggtagat tgtcgcacct
     9241 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     9301 tttaatcgcg gccttgagca agacgtttcc cgttgaatat ggctcataac agaacttatt
     9361 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
     9421 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
     9481 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg
     9541 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
     9601 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
     9661 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
     9721 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
     9781 ggcaggatat attgtggtgt aaacataaca agcttcgtct cagtca
//
