LOCUS       sequence-405887-        3156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription vector for production of synthetic mRNA 
            encoding for mouse Myod1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Qabrati X, Kim I, Ghosh A, Bundschuh N, Noe F, Palmer AS, Bar-Nur O
  TITLE     Transgene-free direct conversion of murine fibroblasts into 
            functional muscle stem cells.
  JOURNAL   NPJ Regen Med. 2023 Aug 8;8(1):43. doi: 10.1038/s41536-023-00317-z.
  PUBMED    37553383
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "NPJ Regen 
            Med."; date: "2023-08-8"; volume: "8(1):43. doi"; pages: " 
            10.1038/s41536-023-00317-z"
COMMENT     Sequence Label: pT7[mRNA]-5'UTR:mMyod1[NM_010866.2]: Hba-a1_3'UTR
FEATURES             Location/Qualifiers
     source          1..3156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1166..1183)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1337..1925)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1417..1436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2096..2887,2888..2956))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2719..2738
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2957..3061)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3129..3147
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 taatacgact cactataggg aaataagaga gaaaagaaga gtaagaagaa atataagagc
       61 caccatggag cttctatcgc cgccactccg ggacatagac ttgacaggcc ccgacggctc
      121 tctctgctcc tttgagacag cagacgactt ctatgatgac ccgtgtttcg actcaccaga
      181 cctgcgcttt tttgaggacc tggacccgcg cctggtgcac atgggagccc tcctgaaacc
      241 ggaggagcac gcacacttcc ctactgcggt gcacccaggc ccaggcgctc gtgaggatga
      301 gcatgtgcgc gcgcccagcg ggcaccacca ggcgggtcgc tgcttgctgt gggcctgcaa
      361 ggcgtgcaag cgcaagacca ccaacgctga tcgccgcaag gccgccacca tgcgcgagcg
      421 ccgccgcctg agcaaagtga atgaggcctt cgagacgctc aagcgctgca cgtccagcaa
      481 cccgaaccag cggctaccca aggtggagat cctgcgcaac gccatccgct acatcgaagg
      541 tctgcaggct ctgctgcgcg accaggacgc cgcgccccct ggcgccgctg ccttctacgc
      601 acctggaccg ctgcccccag gccgtggcag cgagcactac agtggcgact cagatgcatc
      661 cagcccgcgc tccaactgct ctgatggcat gatggattac agcggccccc caagcggccc
      721 ccggcggcag aatggctacg acacagccta ctacagtgag gcggcgcgcg agtccaggcc
      781 agggaagagt gcggctgtgt cgagcctcga ctgcctgtcc agcatagtgg agcgcatctc
      841 cacagacagc cccgctgcgc ctgcgctgct tttggcagat gcaccaccag agtcgcctcc
      901 gggtccgcca gagggggcat ccctaagcga cacagaacag ggaacccaga ccccgtctcc
      961 cgacgccgcc cctcagtgtc ctgcaggctc aaaccccaat gcgatttatc aggtgctttg
     1021 agctgccttc tgcggggctt gccttctggc catgcccttc ttctctccct tgcacctgta
     1081 cctcttggtc tttgaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaatgaag agccgtacgg
     1141 gcgcgcctag gcgcgattcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1201 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1261 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1321 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1381 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1441 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1501 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1561 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1621 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1681 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1741 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1801 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1861 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1921 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1981 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2041 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2101 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2161 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2221 ctgcaatgat accgcgagat ccacgctcac cggctccaga tttatcagca ataaaccagc
     2281 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2341 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2401 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2461 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2521 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2581 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2641 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2701 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2761 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2821 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2881 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2941 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3001 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3061 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     3121 cctataaaaa taggcgtatc acgaggccct ttcgtc
//
